Papers - IWANAMI Toru
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Distribution and infection rate of satsuma dwarf virus in Mie Prefecture, Japan
Nakajima Kaori, Ichinokiyama Hiromichi, Tomimura Kenta, Susaki Noritaka, Iwanami Toru
Annual Report of The Kansai Plant Protection Society 66 ( 0 ) 46 - 54 2024.06
Language：English Publisher：The Kansai Plant Protection Society
<p>Strains of satsuma dwarf virus (SDV) occurring in Mie Prefecture were examined, and at the same time, the disease symptoms on the rind of citrus mosaic virus (CiMV) sub-strain were also examined. SDV-free trifoliate orange (<i>Poncirus trifoliata</i> (L.) Raf.) rootstocks were planted in plots where the SDV strain, the CiMV sub-strain, and the navel orange infectious mottling virus (NIMV) strain occur. The relationship between the SDV strain and the infection rate was determined. The CiMV sub-strain occurred most frequently in Mie Prefecture, followed by the SDV strain. An isolate of the NIMV strain was detected in two orchards in Mie Prefecture. This is the second occurrence of the NIMV strain recorded in a field outside of the original field in Wakayama Prefecture, following an occurrence in Ehime Prefecture. This is also the first report of mixed CiMV sub-strain and NIMV strain infection in citrus. The results indicate that the CiMV sub-strain is widely distributed outside Wakayama Prefecture. Moreover, isolates of the CiMV sub-strain, which hardly induce mosaic symptoms in the fruit rind, were widely distributed. Trifoliate orange seedlings were planted near infected citrus trees growing on trifoliate orange rootstocks. SDV infection occurred within one year of planting. Whether the difference in infection rate was caused by differences in viral strains or by environmental factors in the field remains unknown.</p>
DOI： 10.4165/kapps.66.46
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Citrus Viroids and Minor Citrus Viruses in Japan
IWANAMI Toru
Japan Agricultural Research Quarterly: JARQ 57 ( 3 ) 195 - 204 2023.07
Language：English Publisher：Japan International Research Center for Agricultural Sciences
<p>Citrus species grown in Japan are affected by several viruses and viroids, including satsuma dwarf virus, citrus vein enation virus, citrus exocortis viroid, and other citrus viroids. Recently, a comprehensive review was made on the occurrence, history, and research of the following three major citrus viruses in Japan: satsuma dwarf virus, citrus tatter leaf virus (apple stem grooving virus), and citrus tristeza virus (Iwanami 2022). As a sequel, this study reviews the occurrence, history, and research development of citrus viroids as well as minor citrus viruses in and around Japan.</p>
DOI： 10.6090/jarq.57.195
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IWANAMI Toru
Japan Agricultural Research Quarterly: JARQ 57 ( 2 ) 99 - 110 2023.04
Language：English Publisher：Japan International Research Center for Agricultural Sciences
<p>Citrus species grown in Japan are affected by three major viruses: satsuma dwarf virus (SDV), citrus tatter leaf virus (CTLV, apple stem grooving virus), and citrus tristeza virus (CTV). SDV has spread to other countries, such as China, Korea, and Turkey, apparently through unchecked budwoods and nursery trees; it is natural to assume that some of these contaminated plants harbored CTLV and CTV as well. Thus, citrus viruses in Japan are not only a domestic problem, they are also transboundary threats to agricultural production. This article reviews the occurrence and history of SDV, CTLV, and CTV in Japan as well as recent research developments in Japan and other countries.</p>
DOI： 10.6090/jarq.57.99
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Occurrence and Control of Citrus Greening (Huanglongbing) in Japan
IWANAMI Toru
Japan Agricultural Research Quarterly: JARQ 56 ( 2 ) 105 - 120 2022.04
Language：English Publisher：Japan International Research Center for Agricultural Sciences
<p>Citrus greening (huanglongbing) is established in majority of the subtropical and tropical citrus-producing areas of the world. In Japan, the disease causes damage to citrus grown on the subtropical islands and threatens the major citrus production areas on the main islands of Kyushu, Shikoku, and Honshu. Since the initial identification of the disease on a small island near Taiwan, wide-ranging programs and collaboration among scientists, plant quarantine officials, and administrators to combat the disease have been undertaken. This paper reviews the history of the disease and development of countermeasures in Japan. The scientific insight and crucial technical tools that promote control practices are summarized. Implications for the control of the disease in other countries and future research directions are also discussed.</p>
DOI： 10.6090/jarq.56.105
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Plant viruses and viroids in Japan Reviewed
Shin-ichi Fuji, Tomofumi Mochizuki, Mitsuru Okuda, Shinya Tsuda, Satoshi Kagiwada, Ken-Taro Sekine, Masashi Ugaki, Keiko T. Natsuaki, Masamichi Isogai, Tetsuo Maoka, Minoru Takeshita, Nobuyuki Yoshikawa, Kazuyuki Mise, Takahide Sasaya, Hideki Kondo, Kenji Kubota, Yasuyuki Yamaji, Toru Iwanami, Kazusato Ohshima, Kappei Kobayashi, Tatsuji Hataya, Teruo Sano & Nobuhiro Suzuki
Journal of General Plant Pathology 88 105 - 127 2022
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Occurrence and Control of Citrus Greening (Huanglongbing) in Japan Reviewed
Toru Iwanami
Japan Agricultural Research Quarterly 56 ( 2 ) 105 - 120 2022
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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A new method for early detection of latent infection by ‘Candidatus Liberibacter asiaticus’ in citrus trees Reviewed
Kazuki Fujiwara, Kenta Tomimura, Toru Iwanami, Takashi Fujikawa
F1000Research 10 250 2021
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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NAGAOKA-NAKAZONO Eiko, UECHI Nami, KATOH Hiroshi, FUJIKAWA Takashi, MIYATA Shin-ichi, FURUKAWA Tsutomu, ASAKAWA Tomonori, SASAKI Makoto, KUWAHARA Riho, KIM Ok-Kyung, SHINOHARA Hirosuke, IWANAMI Toru
Japan Agricultural Research Quarterly: JARQ 54 ( 4 ) 307 - 316 2020.10
Language：English Publisher：Japan International Research Center for Agricultural Sciences
<p>Citrus tristeza virus (CTV) commonly infects yuzu (<i>Citrus junos</i>) in most parts of the citrus-growing areas in Japan. This study examined the occurrence of CTV in the northernmost commercial citrusproducing areas of Japan. Colonies of the brown citrus aphid (<i>Toxoptera citricidus</i>) were detected within the areas investigated. However, 23 of the 41 trees that were examined by RT-PCR and both or either subject to stem-pitting observation and immunostrip testing were free of CTV. Several CTVfree trees were apparently more than 60 years old. A field trial revealed that all eight uninoculated seedlings tested negative for CTV at 52 months after transplanting. These results suggest that CTVfree yuzu production is possible in the Rikuzen-Takata and Ofunato areas of Japan.</p>
DOI： 10.6090/jarq.54.307
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Limited occurrence of tristeza in yuzu and possible tristeza-free cultivation in the northernmost commercial yuzu-producing areas in Japan Reviewed
Nagaoka-Nakazono, E., Uechi, N., Kato, H., Fujikawa, T., Miyata, S., Furukawa, T., Asakawa, T., Sasaki, M., Kuwahara, R., Kim, O-K., Shinohara, H. and Iwanami, T.
Japan Agricultural Research Quarterly 54 307 - 316 2020
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Complete Genome Sequence of Rehmannia Mosaic Virus Infecting Rehmannia glutinosa in Japan Reviewed
Naoki Komiyama, Kohei Sakuta, Masayuki Mikage, Hirosuke Shinohara, Toru Iwanami, Hiromitsu Negishi, Ok-Kyung Kim
Microbiology Resource Announcements 10 2020
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Management of Huanglongbing (HLB) by an intensive vector and disease control in the surroundings of the orchard in addition to planting HLB-free trees in Okinawa, Japan
Nami Uechi, Hiroshi Katoh, Toru Iwanami, Takashi Fujikawa, Shin-ichi Miyata, Keiji Yasuda, Tsuyoshi Ooishi, Shinji Kawano, Takumi Uesato
Japan Agricultural Research Quarterly 53 ( 2 ) 103 - 108 2019
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Emergence of apple bacterial quick decline caused by Dickeya dadantii in Japan Reviewed
Takashi Fujikawa, Nobuyoshi Ota, Makoto Sasaki, Taiki Nakamura & Toru Iwanami
Journal of General Plant Pathology 85 314 - 319 2019
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Alterations of Candidatus Liberibacter asiaticus-Associated Microbiota Decrease Survival of Ca. L. asiaticus in in vitro Assays Reviewed
Fujiwara Kazuki, Iwanami Toru, Fujikawa Takashi
Frontiers in Microbiology 9 2018
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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ウンシュウミカンにおける茎頂接ぎ木による3種ウイロイドの効率的無毒化法 Reviewed
中嶋香織・市ノ木山浩道・長岡（中園）栄子・岩波 徹
園芸学研究 16 ( 3 ) 339 - 344 2017.07
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
断続熱処理と比較的実施容易な0.2mmの大きさの茎頂を切り出す茎頂接ぎ木方により、ウンシュウミカンから重要な3種のウイロイドを除去する技術を開発した。また、同法を用いて、有望新品種「みえ紀南3号」の無毒化に成功した。
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No seed transmission of Plum pox virus in Japanese aprico Reviewed
Toru Iwanami, Kenta Tomimura
Japan Agricultural Research Quarterly 51 ( 3 ) 217 - 220 2017.07
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
世界中で甚大な被害を出している核果類の重要ウイルスであるプラムポックスウイルス（ウメ輪紋ウイルス）は日本のウメでは種子伝染せず、感染地域からのウメ果実の出荷は植物防疫上問題が無いことを明らかにした。
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Nucleotide Sequence of Japanese Isolates of Citrus Vein Enation Reviewed
Eiko Nakazono-Nagaoka,Takashi Fujikawa,Toru Iwanami
Archives of Virology 162 ( ) 879 - 883 2017.03
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
日本のカンキツに広く分布するカンキツベインエネーションウイルスの全ゲノムの塩基配列を明らかにし、スペインで報告されているウイルスと極めて相同性が高いが、日本株に特異的な変異も数カ所存在することを明らかにした。コレスポンディングオーサー
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Effective molecular detection of Diaphorina citri Kuwayama (Hemiptera: Liviidae) in bulk insect samples from sticky traps Reviewed
Fujiwara, K., Uechi, N., Shimizu, Y., Inoue, H., Yamaguchi, T., Iwanami, T., and Fujikawa, T.
Journal of Applied Entomology 141 ( ) 61 - 66 2016.03
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
粘着トラップについた残骸から、ミカンキジラミのDNAを検出し、発生の有無を確認する方法を開発した。これにより、カンキツグリーニング病の媒介虫であるミカンキジラミのモニタリングが非常に容易にできるようになった。
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カンキツベインエネーションウイルスによるユズのベインエネーション症状 Reviewed
長岡（中薗）栄子・藤川貴史・上地奈美・岩波 徹
日本植物病理学会報 81 ( 4 ) 341 - 345 2015.11
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
世界的にもその発生生態が不明な点が多いカンキツベインエネーションウイルスが我が国のユズに感染し、葉にひだ葉症状を起こすことを証明した。コレスポンディングオーサー
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Changes in variable number of tandem repeats in ‘Candidatus Liberibacter asiaticus’ through insect transmission Reviewed
Hiroshi Katoh, Hiromitsu Inoue and Toru Iwanami
Plos One 10 ( 9 ) e0138699 2015.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
世界中で甚大な被害をもたらしているカンキツグリーニング病の病原細菌のゲノムの単純反復配列を解析し、ミカンキジラミによって媒介されることで、単純反復配列が大きく変化することを世界で初めて明らかにした。これにより、新規発生地域などで、媒介虫によって拡散したのか、或いは人為的に取り木などで拡散したのかを推定することが可能となった。
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イムノクロマト診断キットによる温州萎縮ウイルスの周年検定 Reviewed
加藤光弘・影山智津子・石井香奈子・上西啓資・藤田絢香・草野成夫・野口真弓・岩波 徹
日本植物病理学会報 80 222 - 228 2014.11
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
温州萎縮病を引き起こす温州萎縮ウイルス(SDV)の被害防止のためには、検定を行い健全な苗木を流通させることが重要であるが、SDVクロマトによる検定に新梢，花蕾，果皮，冬季の切り枝から発生させた新梢や花蕾を用いることでSDVの周年検出が可能となり、健全苗木が流通できる可能性がより高くなった。