Papers - IWATA Hisataka
-
Effects of a gel culture system made of polysaccharides (xanthan gum and locust bean gum) on in vitro bovine oocyte development and gene expression of the granulosa cells. Reviewed
Sugimoto A, Inoue Y, Tanaka K, Sinozawa A, Shirasuna K, Iwata H
Molecular Reproduction and Development 88 ( 7 ) 516 - 524 2021.07
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1002/mrd.23518.
-
Resveratrol enhanced mitochondrial recovery from cryopreservation-induced damages in oocytes and embryos Invited Reviewed
Hisataka Iwata
Reprod Med Biol 20 ( 4 ) 419 - 426 2021.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1002/rmb2.12401.
-
Effect of maternal aging and vitrification on mitochondrial DNA copy number in embryos and spent culture medium. Reviewed
Aoki S, Ito J, Hara S, Shirasuna K, Iwata H.
Reproductive Biology 21 ( 2 ) 100506 2021.06
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
-
Mitochondrial reactive oxygen species regulate mitochondrial biogenesis in porcine embryos Reviewed
Kageyama M, Ito J, Shirasuna K, Kuwayama T, Iwata H
Journal of Reproduction and Development 67 ( 2 ) 141 - 147 2021.04
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
-
凍結に由来するミトコンドリア障害からの胚の回復 Invited Reviewed
伊藤 洵、 岩田 尚孝
Journal of mammalian Ova Research 38 ( 1 ) 25 - 29 2021.04
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
-
Effects of a gel culture system made of polysaccharides on in vitro bovine oocyte development
SUGIMOTO Ayaka, INOUE Yuuki, TANAKA Keisuke, IWATA Hisataka
The Journal of Reproduction and Development Supplement 114 ( 0 ) OR-5 - OR-5 2021
Language：Japanese Publisher：The Society for Reproduction and Development
-
Substrate made of polysaccharides improves bovine oocyte maturation and subsequent embryo development
HARA Shunsuke, INOUE Yuki, TANAKA Keisuke, NOGUCHI Tatsuo, SIRASUNA Koumei, IWATA Hisataka
The Journal of Reproduction and Development Supplement 114 ( 0 ) OR-6 - OR-6 2021
Language：Japanese Publisher：The Society for Reproduction and Development
-
Age associated change of miRNA in bovine follicular fluids and effect of these miRNAs on growth of oocytes derived from early antral follicles.
NAGATA Shuuta, YAMAGUCHI Hitoki, INOUE Yuki, TANAKA Keisuke, SHIRASUNA Koumei, IWATA Hisataka
The Journal of Reproduction and Development Supplement 114 ( 0 ) OR-3 - OR-3 2021
Language：Japanese Publisher：The Society for Reproduction and Development
-
miRNA in bovine oviductal fluid affect embryo development
AOKI Sogo, INOUE Yuki, ISHIDA Daiki, TANAKA Keisuke, NOGUCHI Tatsuo, SHIRASUNA Koumei, IWATA Hisataka
The Journal of Reproduction and Development Supplement 114 ( 0 ) AW-7 - AW-7 2021
Language：Japanese Publisher：The Society for Reproduction and Development
-
Age associated dynamics of mitochondrial gene expression in oocyte, embryo, and granulosa cells determined by RNA-seq
INOUE Yuki, TANAKA Keisuke, IWATA Hisataka
The Journal of Reproduction and Development Supplement 114 ( 0 ) P-6 - P-6 2021
Language：Japanese Publisher：The Society for Reproduction and Development
-
Mitochondrial reactive oxygen species regulate mitochondrial biogenesis in porcine embryos International journal
KAGEYAMA Mio, ITO Jun, SHIRASUNA Koumei, KUWAYAMA Takehito, IWATA Hisataka
Journal of Reproduction and Development 67 ( 2 ) 141 - 147 2021
Language：English Publishing type：Research paper (scientific journal) Publisher：The Society for Reproduction and Development
<p> The number of mitochondria in blastocysts is a potential marker of embryo quality. However, the molecular mechanisms governing the mitochondrial number in embryos are unclear. This study was conducted to investigate the effect of reduced mitochondrial reactive oxygen species (ROS) levels on mitochondrial biogenesis in porcine embryos. Oocytes were collected from gilt ovaries and activated to generate over 4 cell-stage embryos at day 2 after activation. These embryos were cultured in media containing either 0.1 μM MitoTEMPOL (MitoT), 0.5 μM Mitoquinol (MitoQ), or vehicle (ethanol) for 5 days to determine the rate of development to the blastocyst stage. The mitochondrial number in blastocysts was evaluated by real-time polymerase chain reaction (PCR). Five days after activation, the embryos (early morula stage) were subjected to immunostaining to determine the expression levels of NRF2 in the nucleus. In addition, the expression levels of <i>PGC1α</i> and <i>TFAM</i> in the embryos were examined by reverse transcription PCR. One day of incubation with the antioxidants reduced the ROS content in the embryos but did not affect the rate of development to the blastocyst stage. Blastocysts developed in medium containing MitoT had lower mitochondrial DNA copy numbers and ATP content, whereas MitoQ showed similar but insignificantly trends. Treatment of embryos with either MitoT or MitoQ decreased the expression levels of NRF2 in the nucleus and levels of <i>PGC1α</i> and <i>TFAM</i>. These findings indicate that reductions in mitochondrial ROS levels are associated with low mitochondrial biogenesis in embryos.</p>
DOI： 10.1262/jrd.2020-111
-
HIRATA Yoshiki, KATSUKURA Yusuke, HENMI Yuka, OZAWA Ren, SHIMAZAKI Sayaka, KUROSAWA Akira, TORII Yasushi, TAKAHASHI Hironori, IWATA Hisataka, KUWAYAMA Takehito, SHIRASUNA Koumei
Journal of Reproduction and Development 67 ( 4 ) 257 - 264 2021
Language：English Publisher：The Society for Reproduction and Development
<p> Advanced maternal age is a risk factor for female infertility, and placental dysfunction is considered one of the causes of pregnancy complications. We investigated the effects of advanced maternal aging on pregnancy outcomes and placental senescence. Female pregnant mice were separated into three groups: young (3 months old), middle (8–9 months old), and aged (11–13 months old). Although the body weights of young and middle dams gradually increased during pregnancy, the body weight of aged dams only increased slightly. The placental weight and resorption rate were significantly higher, and live fetal weights were reduced in a maternal age-dependent manner. Although mRNA expression of senescence regulatory factors (p16 and p21) increased in the spleen of aged dams, mRNA expression of p16 did not change and that of p21 was reduced in the placenta of aged dams. Using a cytokine array of proteins extracted from placental tissues, the expression of various types of senescence-associated secretory phenotype (SASP) factors was decreased in aged dams compared with young and middle dams. The aged maternal placenta showed reduced immune cell accumulation compared with the young placenta. Our present results suggest that models using pregnant mice older than 8 months are more suitable for verifying older human pregnancies. These findings suggest that general cellular senescence programs may not be included in the placenta and that placental functions, including SASP production and immune cell accumulation, gradually decrease in a maternal age-dependent manner, resulting in a higher rate of pregnancy complications.</p>
DOI： 10.1262/jrd.2021-034
-
Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos. Reviewed
Kobayashi M, Ito J, Shirasuna K, Kuwayama T, Iwata H.
Journal of Reproduction and Development 66 ( 6 ) 539 - 546 2020.12
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
-
ウシの分娩前後における血漿中ミトコンドリア由来細胞外DNA量の変化と血液成分値との関係 Reviewed
野口 龍生, 相澤 拓朗, 岩田 尚孝
日本畜産学会報 91 ( 3 ) 227 - 232 2020.10
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
-
Prediction of major microRNAs in follicular fluid regulating porcine oocyte development Reviewed
Yuki Inoue, Yasuhisa Munakata, Akihisa Shinozawa, Ryoka Kawahara-Miki, Koumei Shirasuna, Hisataka Iwata
Journal of Assisted Reproduction and Genetics 37 ( 10 ) 2569 - 2579 2020.10
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
-
Xanthan gum and locust bean gum substrate improves bovine embryo developmen Reviewed
Hara S, Aoki S, Nagata M, Shirasuna K, Noguchi T, Iwata H.
Reproduction in Domestic Animals. 55 ( 9 ) 1124 - 1131 2020.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1111/rda.13750.
-
Cell free mitochondrial DNA in plasma and physical condition of cows
NOGUCHI Tatsuo, AIZAWA Takuro, IWATA Hisataka
Nihon Chikusan Gakkaiho 91 ( 3 ) 227 - 232 2020.08
Language：Japanese Publisher：Japanese Society of Animal Science
<p>Blood contains cell free DNA (cfDNA), and cell free mitochondrial DNA(cf-mtDNA) is able to be easily detected by real time PCR. The present study investigated the relationship between the amount of cf-mtDNA in plasma and physiological conditions of cows. In addition, relationship between preg-nancy following artificial insemination and concentration of cf-mtDNA in the corresponding plasma. Significantly positive relationship was found between concentration of LDH and cf-mtDNA, and negative relationship was found between concentration of triglyceride and cf-mtDNA. There was no significant difference in the amount of cf-mtDNA between pregnant or non-pregnant cows following artificial insemination.</p>
-
Abundance of cell-free mitochondrial DNA in spent culture medium associated with morphokinetics and blastocyst collapse of expanded blastocysts. Reviewed
Kobayashi M, Kobayashi J, Shirasuna K, Iwata H
Reproductive Medicine and Biology 19 ( 4 ) 404 - 414 2020.08
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1002/rmb2.12344
-
Comparison of Gene Expression and Mitochondria Number Between Bovine Blastocysts Obtained in Vitro and in Vivo Reviewed
Tatsuo Noguchi, Takuro Aizawa, Yasuhisa Munakata, Hisataka Iwata
Journal of Reproduction and Development 66 ( 1 ) 35 - 39 2020.02
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
-
Resveratrol treatment increases mitochondrial biogenesis and improves viability of porcine germinal-vesicle stage vitrified-warmed oocytes Reviewed
Ito J, Shirasuna K, Kuwayama T, Iwata H.
Cryobiology 93 37 - 43 2020
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)