Papers - HIRONOBU Shiwachi
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Replacement of Water Yam (Dioscorea alata L.) Indigenous Root Endophytes and Rhizosphere Bacterial Communities via Inoculation with a Synthetic Bacterial Community of Dominant Nitrogen-Fixing Bacteria Reviewed
Sumetee Liswadiratanakul, Kosuke Yamamoto, Minenosuke Mastutani, Vatanee Wattanadatsaree, Shunta Kihara, Yuh Shiwa and Hironobu Shiwachi
Frontiers in Microbiology. 2023.02
Authorship：Last author Language：English Publishing type：Research paper (scientific journal)
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Biomass production and nutrient use efficiency in white Guinea yam (Dioscorea rotundata Poir) genotypes grown under contrasting soil mineral nutrient availability Reviewed International coauthorship International journal
Ryo Matsumoto, Asrat Asfaw, Haruki Ishikawa, Kanako Takada, Hironobu Shiwachi, Robert Asiedu
Frontiers in Plant Science. 2022.10
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：https://www.frontiersin.org
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Bacterial community of water yam (Dioscorea alata L.) cv. A-19 Reviewed International journal
Kihara, Shunta, Yamamoto Kosuke, Hisatomi Atsushi, Shiwa Yuh, Chu Chia-Cheng, Takada Kanako, Ouyabe Michel, Pachakkil Babil, Kikuno Hidehiko, Tanaka Naoto, Shiwachi Hironobu
Microbes and Environments 37 ( 2 ) ME21062 2022.03
Language：English Publishing type：Research paper (scientific journal)
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Growth of Water Yam (Dioscorea alata L.) as Affected by Bacterial Inoculation under Growth Chamber Conditions. Reviewed
Liswadiratanakul Sumetee, Michel Ouyabe, Hideki Hinomizu, Kanako Takada, Hidehiko Kikuno, Babil Pachakkil and Hironobu Shiwachi
Tropical Agriculture and Development 65 ( 1 ) 41 - 48 2021.03
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Estimation of the amount of nitrogen fixation in white yam by using <sup>15</sup>N dilution technique
Takada Kanako, Matsumoto Ryo, Ishikawa Haruki, Pachakkil Babil, Kikuno Hidehiko, Shiwachi Hironobu
Abstracts of Meeting of the CSSJ 249 ( 0 ) 60 - 60 2020.03
Language：Japanese Publisher：CROP SCIENCE SOCIETY OF JAPAN
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Infection Route of Endophytic Nitrogen-fixing Bacteria in Water Yam (Dioscorea alata L.) Reviewed
Hironobu Shiwachi, Natsumi Miwa, Kanako Takada, Michel Ouyabe, Babil Pachakkil, Hidehiko Kikuno, and Naoto Tanaka
Tropical Agriculture and Development 64 ( 1 ) 1 - 6 2020.03
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.11248/jsta.64.1
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Response of upland rice (Oryza sativa L.) inoculated with non-native plant growth-promoting bacteria Reviewed
Michel Ouyabe, Irie Kenji, Tanaka Naoto, Hidehiko Kikuno, Babil Pachakkil and Hironobu Shiwachi
Agronomy 10 2020
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Michel Ouyabe, Naoto Tanaka, Yuh Shiwa, Nobuyuki Fujita, Hidehiko Kikuno, Pachakkil Babil and Hironobu Shiwachi
International Journal of Systematic and Evolutionary Microbiology 70 ( 9 ) 5054 - 5062 2020
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Microbiology Society
<jats:p>This study investigated endophytic nitrogen-fixing bacteria isolated from two species of yam (water yam, <jats:italic>Dioscorea alata</jats:italic> L.; lesser yam, <jats:italic>Dioscorea esculenta</jats:italic> L.) grown in nutrient-poor alkaline soil conditions on Miyako Island, Okinawa, Japan. Two bacterial strains of the genus <jats:italic>
<jats:named-content content-type="genus">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1279" xlink:type="simple">Rhizobium</jats:ext-link>
</jats:named-content>
</jats:italic>, S-93<jats:sup>T</jats:sup> and S-62, were isolated. The phylogenetic tree, based on the almost-complete 16S rRNA gene sequences (1476 bp for each strain), placed them in a distinct clade, with <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13566" xlink:type="simple">Rhizobium miluonense</jats:ext-link>
</jats:named-content>
</jats:italic> CCBAU 41251<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1287" xlink:type="simple">Rhizobium hainanense</jats:ext-link>
</jats:named-content>
</jats:italic> I66<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13567" xlink:type="simple">Rhizobium multihospitium</jats:ext-link>
</jats:named-content>
</jats:italic> HAMBI 2975<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.24765" xlink:type="simple">Rhizobium freirei</jats:ext-link>
</jats:named-content>
</jats:italic> PRF 81<jats:sup>T</jats:sup> and <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1306" xlink:type="simple">Rhizobium tropici</jats:ext-link>
</jats:named-content>
</jats:italic> CIAT 899<jats:sup>T</jats:sup> being their closest species. Their bacterial fatty acid profile, with major components of C<jats:sub>19 : 0</jats:sub> cyclo <jats:italic>ω</jats:italic>8<jats:italic>c</jats:italic> and summed feature 8, as well as other phenotypic characteristics and DNA G+C content (59.65 mol%) indicated that the novel strains belong to the genus <jats:italic>
<jats:named-content content-type="genus">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1279" xlink:type="simple">Rhizobium</jats:ext-link>
</jats:named-content>
</jats:italic>. Pairwise average nucleotide identity analyses separated the novel strains from their most closely related species with similarity values of 90.5, 88.9, 88.5, 84.5 and 84.4 % for <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13567" xlink:type="simple">R. multihospitium</jats:ext-link>
</jats:named-content>
</jats:italic> HAMBI 2975<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1306" xlink:type="simple">R. tropici</jats:ext-link>
</jats:named-content>
</jats:italic> CIAT 899<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1287" xlink:type="simple">R. hainanense</jats:ext-link>
</jats:named-content>
</jats:italic> CCBAU 57015<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13566" xlink:type="simple">R. miluonense</jats:ext-link>
</jats:named-content>
</jats:italic> HAMBI 2971<jats:sup>T</jats:sup> and <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.24765" xlink:type="simple">R. freirei</jats:ext-link>
</jats:named-content>
</jats:italic> PRF 81<jats:sup>T</jats:sup>, respectively; digital DNA–DNA hybridization values were in the range of 26–42 %. Considering the phenotypic characteristics as well as the genomic data, it is suggested that strains S-93<jats:sup>T</jats:sup> and S-62 represent a new species, for which the name <jats:italic>Rhizobium dioscoreae</jats:italic> is proposed. The type strain is S-93<jats:sup>T</jats:sup> (=NRIC 0988<jats:sup>T</jats:sup>=NBRC 114257<jats:sup>T</jats:sup>=DSM 110498<jats:sup>T</jats:sup>).</jats:p> -
Isolation of Nitrogen-Fixing Bacteria from Water Yam (Dioscorea alata L.) Reviewed
Kanako TAKADA, Naoto TANAKA, Hidehiko KIKUNO, Pachakkil BABIL, Michio ONJO, Byoung-Jae PARK, and Hironobu SHIWACHI
Tropical Agriculture and Development. 63 63 ( 4 ) 198 - 203 2019.12
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Contribution of Biological Nitrogen Fixation in Lesser Yam (Dioscorea esculenta L.) Associated with Endophytic Diazotrophic Bacteria Reviewed
Michel OUYABE, Hidehiko KIKUNO, Naoto TANAKA, Pachakkil BABIL, Hironobu SHIWACH
Tropical Agriculture and Development 63 ( 3 ) 131 - 139 2019.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Endophytic Nitrogen-Fixing Bacteria of Water Yam (Dioscorea alata L.) in Relation with Fertilization Practices Reviewed
Michel OUYABE, Hidehiko KIKUNO, Naoto TANAKA, Pachakkil BABIL, Hironobu SHIWACH
Tropical Agriculture and Development 63 ( 3 ) 122 - 130 2019.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Isolation and identification of nitrogen-fixing bacteria associated with Dioscorea alata L. and Dioscorea esculenta L Reviewed
Michel OUYABE, Hidehiko KIKUNO, Naoto TANAKA, Pachakkil BABIL, Hironobu SHIWACH
Microbial Resources and Systematics 35 ( 1 ) 3 - 11 2019.06
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Bulbil dormancy and formation in water yam (Dioscorea alata L.) Reviewed
Atsushi Sanada, Chuwen Cheng, Hidehiko Kikuno and Hironobu Shiwachi
Tropical Agriculture and Development 62 ( 3 ) 109 - 114 2018.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Analysis of the source of nitrogen during water yam (Dioscorea alata L.) growth using δ15N observations Reviewed
Kanako Takada, Hidehiko Kikuno, Pachakkil Babil and Hironobu Shiwachi
Tropical Agriculture and Development 62 ( 3 ) 124 - 131 2018.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Intra –Specific Genetic Diversity in Dioscorea alata accessions collected from Myanmar, Papua New Guinea and Japan. Reviewed
Favour OLU-OLUSEGUN, Pachakkil BABIL, Hidehiko KIKUNO, Kenji IRIE, Hidekazu TOYOHARA, and Hironobu SHIWACHI
Journal of International Society for Southeast Asian Agricultural Sciences 24 ( 1 ) 107 - 117 2018.06
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Water yam (Dioscorea alata L.) is able to grow in low fertile soil conditions Reviewed
Kanako TAKADA, Hidehiko KIKUNO, Pachakkil BABIL, Kenji IRIE and Hironobu SHIWACHI
Tropical Agriculture and Development 61 ( 1 ) 8 - 14 2017.03
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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The nitrogen-fixing endophytes bacterium associated with the lesser yam (Dioscorea esculenta L.) Reviewed
Abdul Qayom Rezaei, Hidehiko Kikuno, Pachakkil Babil, Naoto Tanaka, Byoung-Jae Parak, Michio Onjo and Hironobu Shiwachi
Tropical Agriculture and Development 61 ( 1 ) 40 - 47 2017.03
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Phylogenetic Relationship of Indonesian Water Yam (Dioscorea alata L.) Cultivars Based on DNA Marker Using ITS-rDNA Analysis Reviewed
Purnomo, Budi Setiadi Daryono and Hironobu Shiwachi
Journal of Agricultural Science 9 ( 2 ) 154 - 161 2017.01
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Somatic polyploidization and characterization of induced polyploids of Dioscorea rotundata and Dioscorea cayenensis Reviewed
Pachakkil Babil1, Midori Iino, Yukiko Kashihara, Ryo Matsumoto, Hidehiko Kikuno,Antonio Lopez-Montes and Hironobu Shiwachi
African Journal of Biotechnology 15 ( 38 ) 2098 - 2105 2016.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Effect of Nitrogen Fertilizer on Growth of the Lesser Yam (Dioscorea esculenta L.) Reviewed
Abdul Qayom Rezaei, Hidehiko Kikuno, Kaoru Sugiyama, Pachakkil Babil, Byoung-Jae Parak, Michio Onjo and Hironobu Shiwachi
Tropical Agriculture and Development 60 ( 3 ) 185 - 190 2016.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
The effect of fertilization on lesser yam (Dioscorea esculenta L.) growth is not clearly understood. Increased lesser
yam production in Okinawa prefecture, Japan, is desired due to increasing demand for use in commercial health supplements.
However, fertilization guidelines for production are not yet available in Japan. This study aimed to reveal the effects of nitrogen application on growth of lesser yam in Okinawa from April 2014 to January 2015. Growth with urea application (N treatment)and without urea (control) were compared. N treatment had a positive effect on growth and tuber yield in soil with poor organic matter. Yellowish leaves were observed in control plants from 120 days after planting (DAP), but plants grew until 240 DAP. It was possible to grow lesser yams under poor fertile soil conditions. The δ15N values of the N treated plants suggested that nitrogen was absorbed from the urea; however, the source of nitrogen in the control plants was not identified. The quantity of nitrogen required for a stable yield of lesser yam is thought to be less than that required for water yam (D. alata).