論文 - 相根 義昌
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Data on the weights, specific gravities and chemical compositions of potato (Solanum tuberosum) tubers for food processing from different areas of Hokkaido, Japan. 査読あり
Sato H, Koizumi R, Nakazawa Y, Yamazaki M, Itoyama R, Ichisawa M, Negichi J, Sakuma R, Furusho T, Sagane Y, Takano K
Data in brief 11 601 - 605 2017年04月
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Flow-mediated vasodilation response to ingestion of Omega-3-rich Sacha-inchi oil: A noninvasive evaluation of a functional food for human vascular. 招待あり 査読あり
Minami, K., Kashimura, O., Maezaki, Y., Kiyoyanagi, N., Niwa, K., Sagane, Y., Watanabe, T.
Food Preservation Science 43 163 - 170 2017年04月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Building-block architecture of botulinum toxin complex: Conformational changes provide insights into the hemagglutination ability of the complex. 査読あり
Suzuki T, Sagane Y, Matsumoto T, Hasegawa K, Yamano A, Niwa K, Watanabe T
Biochemistry and biophysics reports 9 67 - 71 2017年03月
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Data on volatile compounds in fermented materials used for salmon fish sauce production. 査読あり
Nakano, M., Sagane, Y., Koizumi, R., Nakazawa, Y., Yamazaki, M., Watanabe, T., Takano, K., Sato, H. (2017).
Data Brief 16 1006 - 1014 2017年02月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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ナノカプセルを処方したエミューオイル含有保湿剤「エミュランス EE セラム」の安全性検討 査読あり
伊藤 実, 南 和広, 高野 圭司, 西谷 美雪, 相根 義昌, 渡部 俊弘, 丹羽 光一
皮膚の科学 16 88 - 92 2017年01月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Tomonori Suzuki, Thomas Nagano, Koichi Niwa, Shingo Mutoh, Masataka Uchino, Motohiro Tomizawa, Yoshimasa Sagane, Toshihiro Watanabe
Microbiological Research 193 20 - 29 2016年12月
掲載種別：研究論文（学術雑誌）
© 2016 Elsevier GmbHBotulinolysin (BLY) is a toxin produced by Clostridium botulinum that belongs to a group of thiol-activated hemolysins. In this study, a protein exhibiting hemolytic activity was purified from the culture supernatant of C. botulinum serotype D strain 4947. The purified protein displayed a single band by sodium dodecyl sulfate polyacrylamide gel electrophoresis with a molecular mass of 55 kDa, and its N-terminal and internal amino acid sequences exhibited high similarity to a group of thiol-activated hemolysins produced by gram-positive bacteria. Thus, the purified protein was identified as the BLY. Using the nucleotide sequences of previously cloned genes for hemolysins, two types of genes encoding BLY-like proteins were cloned unexpectedly. Molecular modeling analysis indicated that the products of both genes displayed very similar structures, despite the low sequence similarity. In silico screening revealed a specific duplication of the hemolysin gene restricted to serotypes C and D of C. botulinum and their related species among thiol-activated hemolysin-producing bacteria. Our findings provide important insights into the genetic characteristics of pathogenic bacteria.
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Data on melanin production in B16F1 melanoma cells in the presence of emu oil. 査読あり
Ito M, Minami K, Sagane Y, Watanabe T, Niwa K
Data in brief 9 1056 - 1059 2016年11月
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Construction of "Toxin Complex" in a Mutant Serotype C Strain of Clostridium botulinum Harboring a Defective Neurotoxin Gene. 査読あり
Suzuki T, Nagano T, Niwa K, Uchino M, Tomizawa M, Sagane Y, Watanabe T
Current microbiology 74 ( 1 ) 49 - 54 2016年10月
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Data describing inhibitory profiles of sugars against hemagglutination by the botulinum toxin complex of Clostridium botulinum serotypes C and D. 査読あり
Sagane Y, Ito M, Miyata K, Suzuki T, Niwa K, Oguri S, Watanabe T
Data in brief 9 413 - 416 2016年09月
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Conformational divergence in the HA-33/HA-17 trimer of serotype C and D botulinum toxin complex. 査読あり
Sagane Y, Hayashi S, Akiyama T, Matsumoto T, Hasegawa K, Yamano A, Suzuki T, Niwa K, Watanabe T, Yajima S
Biochemical and biophysical research communications 476 ( 4 ) 280 - 285 2016年08月
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"Non-Toxic" Proteins of the Botulinum Toxin Complex Exert In-vivo Toxicity. 査読あり
Miyashita S, Sagane Y, Suzuki T, Matsumoto T, Niwa K, Watanabe T
Scientific reports 6 31043 2016年08月
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Hemagglutinin gene shuffling among Clostridium botulinum serotypes C and D yields distinct sugar recognition of the botulinum toxin complex. 査読あり
Miyata K, Suzuki T, Hayashi S, Miyashita S, Ohyama T, Niwa K, Watanabe T, Sagane Y
Pathogens and disease 73 ( 7 ) 2015年10月
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Host-cell specificity and transcytosis of nontoxic nonhemagglutinin protein of botulinum neurotoxin serotype D. 査読あり
Miyashita S, Niwa K, Watanabe T, Sagane Y
FEMS microbiology letters 357 ( 2 ) 115 - 122 2014年08月
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Identification of the interaction region between hemagglutinin components of the botulinum toxin complex. 査読あり
Suzuki T, Miyashita S, Hayashi S, Miyata K, Inui K, Kondo Y, Miyazaki S, Ohyama T, Niwa K, Watanabe T, Sagane Y
International journal of biological macromolecules 65 284 - 288 2014年04月
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Crystallization and preliminary X-ray analysis of a novel haemagglutinin component of the toxin complex of serotype C Clostridium botulinum. 査読あり
Hayashi S, Akiyama T, Sagane Y, Miyashita S, Watanabe T, Yajima S, Niwa K
Acta crystallographica. Section F, Structural biology communications 70 ( Pt 3 ) 370 - 373 2014年03月
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Purification, crystallization and preliminary X-ray analysis of an HA17-HA70 (HA2-HA3) complex from Clostridium botulinum type C progenitor toxin. 査読あり
Iwasa C, Tonozuka T, Shinoda M, Sagane Y, Niwa K, Watanabe T, Yoshida H, Kamitori S, Takao T, Oguma K, Nishikawa A
Acta crystallographica. Section F, Structural biology communications 70 ( Pt 1 ) 64 - 67 2014年01月
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Botulinum Toxin Complex Increases Paracellular Permeability in Intestinal Epithelial Cells via Activation of p38 Mitogen-Activated Protein Kinase 査読あり
Miyashita Shin-ichiro, Sagane Yoshimasa, Inui Ken, Hayashi Shintaro, Miyata Keita, Suzuki Tomonori, Ohyama Tohru, Watanabe Toshihiro, Niwa Koichi
JOURNAL OF VETERINARY MEDICAL SCIENCE 75 ( 12 ) 1637 - 1642 2013年12月
掲載種別：研究論文（学術雑誌）
DOI： 10.1292/jvms.13-0164
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Transport of the botulinum neurotoxin-associating protein, nontoxic nonhemagglutinin, across the rat small intestinal epithelial cell monolayer. 査読あり
Miyashita S, Sagane Y, Niwa K, Watanabe T
FEMS microbiology letters 346 73 - 80 2013年09月
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Sugar-induced conformational change found in the HA-33/HA-17 trimer of the botulinum toxin complex. 査読あり
Sagane Y, Hayashi S, Matsumoto T, Miyashita S, Inui K, Miyata K, Yajima S, Suzuki T, Hasegawa K, Yamano A, Nishikawa A, Ohyama T, Watanabe T, Niwa K
Biochemical and biophysical research communications 438 483 - 487 2013年08月
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Random Phage Display-Based Screening of Peptides that Bind to Botulinum Neurotoxin Binding Protein, Nontoxic Nonhemagglutinin. 査読あり
Sagane Y, Miyata K, Kurihara S, Yoneyama T, Inui K, Miyashita SI, Hayashi S, Suzuki T, Niwa K, Watanabe T
Current microbiology 67 ( 2 ) 188 - 192 2013年03月