Papers - WATANABE Satoru
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Effective plasmid delivery to a plasmid-free Bacillus natto strain by a conjugational transfer system Reviewed International journal
Itaya M, Sato M, Watanabe S, Kataoka M.
J Biochem. 172 ( 5 ) 313 - 319 2022.10
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1093/jb/mvac069.
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Development of a stable semi-continuous lipid production system of an oleaginous Chlamydomonas sp. mutant using multi-omics profiling Reviewed International journal
Oyama T, Kato Y, Hidese R, Matsuda M, Matsutani M, Watanabe S, Kondo A, Hasunuma T.
Biotechnol Biofuels Bioprod . 15 ( 1 ) 95 2022.09
Language：English Publishing type：Research paper (scientific journal)
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Novel heat shock response mechanism mediated by the initiation nucleotide of transcription Reviewed
Sato A, Takamatsu M, Kobayashi S, Ogawa M, Shiwa Y, Watanabe S, Chibazakura T, Yoshikawa H.
J Gen Appl Microbiol. 68 ( 2 ) 95 - 108 2022.09
Language：English Publisher：Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
<p>Among SigA-dependent promoters in <i>Bacillus subtilis</i>, we compared the nucleotide sequences of heat shock responding and non-responding promoters. Chimeric promoter experiments revealed that the heat shock response could be ascribed to the initiation nucleotide (iNTP) of the transcription. Our <i>in vivo</i> reporter assay results indicated that a full response was achieved using GTP, a reduced response was observed using ATP, and no additional expression was observed using UTP or CTP.
We then investigated the <i>in vitro</i> transcription assay in more detail. Enhanced transcription that was dependent upon the iNTP was observed when heat treatment was administered during the pre-initiation period. We next analyzed the efficiency of open complex formation using potassium permanganate footprinting, and our results revealed an increase in the ratio of open complex formation at elevated temperatures. Based on this, we suggest that the overall intensification of transcription at high temperatures was derived from the high efficiency of open complex formation together with the high affinity of RNA polymerase (RNAP) for the initiation nucleotide GTP.
To determine if this mechanism observed in <i>B. subtilis </i>RNAP is common among bacterial species, we performed similar experiments using <i>Escherichia coli</i> RNAP. Our results indicated that <i>E. coli </i>RNAP also exhibited both temperature- and iNTP-dependent enhancement of transcription. Although the temperature ranges and the ratios of enhancement are somewhat different, the overall heat shock response mechanism mediated by the iNTP of transcription appears to be conserved among bacterial RNAP.</p> -
Conserved Two-Component Hik2-Rre1 Signalling is Activated Under Temperature Upshift and Plastoquinone-Reducing Conditions in the Cyanobacterium Synechococcus elongatus PCC 7942. Reviewed
Bairagi N, Watanabe S, Nimura-Matsune K, Tanaka K, Tsurumaki T, Nakanishi S, Tanaka K.
Plant Cell Physiol. 2021.11
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1093/pcp/pcab158.
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Biosynthesis system of Synechan, a sulfated exopolysaccharide, in the model cyanobacterium Synechocystis sp. PCC 6803 Reviewed
Maeda K, Okuda Y, Enomoto G, Watanabe S, Ikeuchi M
eLife 2021.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae Reviewed
Takahashi S, Okubo R, Kanesaki Y, Zhou B, Takaya K, Watanabe S, Tanaka K, Imamura S
Plants 10 ( 5 ) 2021.05
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Visualization of the seasonal shift of a variety of airborne pollens in western Tokyo
Uetake J, Tobo Y, Kobayashi S, Tanaka K, Watanabe S, DeMott PJ, Kreidenweis SM.
Sci Total Environ. 20 ( 788 ) 147623 2021.05
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Evolution of Ribosomal Protein S14 Demonstrated by the Reconstruction of Chimeric Ribosomes in Bacillus subtilis. Reviewed
Akanuma G, Kawamura F, Watanabe S, Watanabe M, Okawa F, Natori Y, Nanamiya H, Asai K, Chibazakura T, Yoshikawa H, Soma A, Hishida T, Kato-Yamada Y.
J Bacteriol. 203 ( 10 ) e00599 2021.04
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1128/JB.00599-20.
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mRNA localization, reaction centre biogenesis and thylakoid membrane targeting in cyanobacteria. Reviewed
Mahbub M, Hemm L, Yang Y, Kaur R, Carmen H, Engl C, Huokko T, Riediger M, Watanabe S, Liu LN, Wilde A, Hess WR, Mullineaux CW.
Nat Plants 6 ( 9 ) 1179 - 1191 2020.09
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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The CRP-family transcriptional regulator DevH regulates expression of heterocyst-specific genes at the later stage of differentiation in the cyanobacterium Anabaena sp. strain PCC 7120.
Kurio Y, Koike Y, Kanesaki Y, Watanabe S, Ehira S.
Mol Microbiol. 114 ( 4 ) 553 - 562 2020.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1111/mmi.14558.
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Kashimoto Tomonori, Miyake Keita, Sato Mayuko, Maeda Kaisei, Matsumoto Chikahiro, Ikeuchi Masahiko, Toyooka Kiminori, Watanabe Satoru, Kanesaki Yu, Narikawa Rei
The Journal of General and Applied Microbiology 66 ( 2 ) 106 - 115 2020.06
Language：English Publisher：Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
<p>The cyanobacterium <i>Acaryochloris marina</i> MBIC 11017 (<i>A. marina</i> 11017) possesses chlorophyll <i>d</i> (Chl. <i>d</i>) peaking at 698 nm as photosystem reaction center pigments, instead of chlorophyll <i>a</i> (Chl. <i>a</i>) peaking at 665 nm. About 95% of the total chlorophylls is Chl. <i>d</i> in <i>A. marina</i> 11017. In addition, <i>A. marina</i> 11017 possesses phycobilisome (PBS) supercomplex to harvest orange light and to transfer the absorbing energy to the photosystems. In this context, <i>A. marina</i> 11017 utilizes both far-red and orange light as the photosynthetic energy source. In the present study, we incubated <i>A. marina</i> 11017 cells under monochromatic orange and far-red light conditions and performed transcriptional and morphological studies by RNA-seq analysis and electron microscopy. Cellular absorption spectra, transcriptomic profiles, and microscopic observations demonstrated that PBS was highly accumulated under an orange light condition relative to a far-red light condition. Notably, transcription of one <i>cpcBA</i> operon encoding the phycobiliprotein of the phycocyanin was up-regulated under the orange light condition, but another operon was constitutively expressed under both conditions, indicating functional diversification of these two operons for light harvesting. Taking the other observations into consideration, we could illustrate the photoacclimation processes of <i>A. marina</i> 11017 in response to orange and far-red light conditions in detail.</p>
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Proteomic analysis of haem-binding protein from Arabidopsis thaliana and Cyanidioschyzon merolae. Reviewed
Shimizu T, Yasuda R, Mukai Y, Tanoue R, Shimada T, Imamura S, Tanaka K, Watanabe S, Masuda T.
Philos Trans R Soc Lond B Biol Sci. 375 ( 1801 ) 20190488 2020.05
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Regulation of the groESL1 transcription by the HrcA repressor and a novel transcription factor Orf7.5 in the cyanobacterium Synechococcus elongatus PCC7942. Reviewed
Saito M, Watanabe S, Nimura-Matsune K, Yoshikawa H, Nakamoto H.
J Gen Appl Microbiol. 66 ( 2 ) 85 - 92 2020.04
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Acclimation process of the chlorophyll d-bearing cyanobacterium Acaryochloris marina to an orange light environment revealed by transcriptomic analysis and electron microscopic observation. Reviewed
Kashimoto T, Miyake K, Sato M, Maeda K, Matsumoto C, Ikeuchi M, Toyooka K, Watanabe S, Kanesaki Y, Narikawa R.
J Gen Appl Microbiol. 66 ( 2 ) 80 - 84 2020.03
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Specific binding of DnaA to the DnaA box motif in the cyanobacterium Synechococcus elongatus PCC 7942. Reviewed
Watanabe S, Saito S, Suezaki Y, Seguchi T, Ohbayashi R.
J Gen Appl Microbiol. 66 ( 2 ) 80 - 84 2020.02
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Novel (p)ppGpp 0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. Reviewed
Osaka N, Kanesaki Y, Watanabe M, Watanabe S, Chibazakura T, Takada H, Yoshikawa H, Asai K.
Mol Microbiol. 113 ( 6 ) 1155 - 1169 2020.02
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1111/mmi.14484.
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The retrograde signaling protein GUN1 regulates tetrapyrrole biosynthesis. Reviewed
Shimizu T, Kacprzak SM, Mochizuki N, Nagatani A, Watanabe S, Shimada T, Tanaka K, Hayashi Y, Arai M, Leister D, Okamoto H, Terry MJ, Masuda T
Proc Natl Acad Sci U S A. 116 ( 49 ) 24900 - 24906 2019.12
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Stable mutants of restriction-deficient/modification-proficient Bacillus subtilis 168: hub strains for giant DNA engineering Reviewed
Itaya M, Sato M, Watanabe S, Yoshikawa H, Tomita M, Sato R.
Journal of Biochemistry 166 ( 3 ) 231 - 236 2019.09
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1093/jb/mvz031.
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Cytosine N4-Methylation via M.Ssp6803II Is Involved in the Regulation of Transcription, Fine-Tuning of DNA Replication and DNA Repair in the Cyanobacterium Synechocystis sp. PCC 6803
Katrin Gärtner, Stephan Klähn, Satoru Watanabe, Stefan Mikkat, Ingeborg Scholz, Wolfgang R. Hess and Martin Hagemann
frontiers in Microbiology 00 ( 00 ) 00 - 00 2019.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Complete Genome Sequence of Enterococcus faecium QU50, a Thermophilic Lactic Acid Bacterium Capable of Metabolizing Xylose. Reviewed
Abe K, Kanesaki Y, Abdel-Rahman MA, Watanabe S, Zendo T, Chibazakura T, Shimizu-Kadota M, Sonomoto K, Yoshikawa H.
Microbiol Research Announcement 23 ( 8 ) e00413 - e00419 2019.05
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)