研究者詳細 - 松島　芳隆

論文 - 松島　芳隆

分割表示 49 件中 1 - 20 件目／全件表示 >>

Application of the SpCas9 inhibitor BRD0539 for CRISPR/Cas9-based genetic tools in Lacticaseibacillus paracasei 査読あり

TAMAKI Mizuna, CHIKU Tsutomu, SUZUKI Shunya, MISAKI Aya, FUNAKUBO Asuka, MATSUSHIMA Yoshitaka, YOKOTA Kenji, IGIMI Shizunobu, KAJIKAWA Akinobu

Bioscience of Microbiota, Food and Health 44 ( 1 ) 70 - 79 2025年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：BMFH Press

Although the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas system has been extensively developed since its discovery for eukaryotic and prokaryotic genome editing and other genetic manipulations, there are still areas warranting improvement, especially regarding bacteria. In this study, BRD0539, a small-molecule inhibitor of Streptococcus pyogenes Cas9 (SpCas9), was used to suppress the activity of the nuclease during genetic modification of Lacticaseibacillus paracasei, as well as to regulate CRISPR interference (CRISPRi). First, we developed and validated a CRISPR-SpCas9 system targeting the sirA gene of L. paracasei. Then BRD0539 was used for CRISPR-dependent DNA cleavage in vivo. Our results suggested that the inhibitor worked partially in both Escherichia coli and L. paracasei. Next, we designed a CRISPRi system in a L. paracasei strain by inserting an inactive SpCas9 gene into the chromosome and introducing a plasmid encoding for a single guide RNA (sgRNA) targeting the sirA gene. Expression of sirA was successfully inhibited in the recombinant strains, and CRISPRi was abolished in an inhibitor-dependent manner. Our findings may help expand the CRISPR toolbox for research on lactic acid bacteria and other microbes.

DOI： 10.12938/bmfh.2024-009

Gram-scale approach for β-costic acid via allylic oxidation of β-selinene 査読あり国際誌

Yoshitaka Matsushima, Kosuke Iwata

Bioscience, Biotechnology, and Biochemistry 88 ( 7 ) 742 - 746 2024年05月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： https://doi.org/10.1093/bbb/zbae052

Gram-scale synthesis of cytoxazone via dihydrooxazine formation through SiO2-promoted intramolecular substitution of a trichloroacetimidate 査読あり国際誌

Yoshitaka Matsushima, Kota Otsuka, Moe Ishikawa, Shu Akita

Tetrahedron Letters 131 154586 2023年10月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： doi.org/10.1016/j.tetlet.2023.154786

Simple synthesis of violaceoid A, a cytotoxic hydroquinone 査読あり国際誌

Yoshitaka Matsushima, Haruka Yoshioka, Naoya Sunada

Tetrahedron Letters 122 154522 2023年04月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.tetlet.2023.154522

Concise synthesis of amino acid component of amicoumacins via dihydrooxazine formation through intramolecular conjugate addition 査読あり国際誌

Yoshitaka Matsushima, Yukako Ogawa, Katsuya Nishi, Kyosuke Nakata

Bioscience, Biotechnology, and Biochemistry 87 ( 2 ) 131 - 137 2023年02月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： doi.org/10.1093/bbb/zbac182

Enantioselective total syntheses of (S)-phosphonothrixin and unexpected cyclic derivative (S)-cyclic phosphonothrixin via enzymatic resolution 査読あり国際誌

Koki Nakamura, Yoshitaka Matsushima

Bioscience, Biotechnology, and Biochemistry 87 ( 2 ) 138 - 147 2023年02月

担当区分：最終著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： doi.org/10.1093/bbb/zbac188

Total synthesis of the methyl glycoside of bradyrhizose via intramolecular pinacol coupling 査読あり国際誌

Yoshitaka Matsushima, Yutaro Ito, Hiroyuki Konno

Tetrahedron Letters 106 154060 2022年08月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.tetlet.2022.154060

Isolation of phenolic acids and tannin acids from Mangifera indica L. kernels as inhibitors of lipid accumulation in 3T3-L1 cells. 査読あり国際誌

Takahiro Fujimaki, Chikako Sato, Rei Yamamoto, Sayo Watanabe, Hikaru Fujita, Hidehiko Kikuno, Masayuki Sue, Yoshitaka Matsushima

Bioscience, Biotechnology and Biochemistry 86 ( 5 ) 665 - 671 2022年04月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1093/bbb/zbac030

Efficient synthesis of zealexin B1, a maize sesquiterpenoid phytoalexin, via Suzuki-Miyaura coupling 査読あり国際共著国際誌

Yoshitaka Matsushima, Kohei Ishii, Alisa Huffaker, Eric A. Schmelz

Tetrahedron Letters 91 153641 2022年01月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.tetlet.2022.153641

Concise and scalable synthesis of (±)-phosphonothrixin 査読あり国際誌

Yoshitaka Matsushima, Koki Nakamura

Results in Chemistry 3 100251 2021年11月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.rechem.2021.100251

新スタンダード栄養・食物シリーズ17「有機化学の基礎」

松島芳隆

化学と生物 59 ( 10 ) 531 - 531 2021年10月

記述言語：日本語出版者・発行元：公益社団法人日本農芸化学会

DOI： 10.1271/kagakutoseibutsu.59.531

強烈なインパクト

松島芳隆

化学と生物 59 ( 8 ) 363 - 363 2021年08月

記述言語：日本語出版者・発行元：公益社団法人日本農芸化学会

DOI： 10.1271/kagakutoseibutsu.59.363

Geospatial Assessments of DNA Adducts in the Human Stomach: A Model of Field Cancerization 査読あり国際誌

Yuji Iwashita, Ippei Ohnishi, Yuto Matsushita, Shunsuke Ohtsuka, Takashi Yamashita, Keisuke Inaba, Atsuko Fukazawa, Hideto Ochiai, Keigo Matsumoto, Nobuhito Kurono, Yoshitaka Matsushima, Hiroki Mori, Shioto Suzuki, Shohachi Suzuki, Fumihiko Tanioka, Haruhiko Sugimura

Cancers 13 3728 2021年07月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.3390/cancers13153728

GABA_A Receptors and Maternally Derived Taurine Regulate the Temporal Specification of Progenitors of Excitatory Glutamatergic Neurons in the Mouse Developing Cortex 査読あり国際誌

Shiro Tochitani, Tomonori Furukawa, Ryo Bando, Shigeaki Kondo, Takashi Ito, Yoshitaka Matsushima, Toshio Kojima, Hideo Matsuzaki, Atsuo Fukuda

Cerebral Cortex 31 4554 - 4574 2021年05月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1093/cercor/bhab106

Concise synthesis of the Taxol side chain and demethoxy-4-epi-cytoxazone via oxazoline formation through intramolecular benzylic substitution of a bis-trichloroacetimidate 査読あり国際誌

Yoshitaka Matsushima, Mina Orita

Tetrahedron Letters 73 153095 2021年05月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.tetlet.2021.153095

Mass spectrometric profiling of DNA adducts in the human stomach associated with damage from environmental factors 査読あり国際誌

Ippei Ohnishi, Yuji Iwashita, Yuto Matsushita, Shunsuke Ohtsuka, Takashi Yamashita, Keisuke Inaba, Atsuko Fukazawa, Hideto Ochiai, Keigo Matsumoto, Nobuhito Kurono, Yoshitaka Matsushima, Hiroki Mori, Shioto Suzuki, Shohachi Suzuki, Fumihiko Tanioka and Haruhiko Sugimura

Genes and Environment 43 2021年04月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1186/s41021-021-00186-2

DNA Adductome Analysis Identifies N‐Nitrosopiperidine Involved in the Etiology of Esophageal Cancer in Cixian, China 査読あり国際誌

Yukari Totsuka, Yingsong Lin, Yutong He, Kousuke Ishino, Haruna Sato, Mamoru Kato, Momoko Nagai, Asmaa Elzawahry, Yasushi Totoki, Hiromi Nakamura, Fumie Hosoda, Tatsuhiro Shibata, Tomonari Matsuda, Yoshitaka Matsushima, Guohui Song, Fanshu Meng, Dongfang Li, Junfeng Liu, Youlin Qiao, Wenqiang Wei, Manami Inoue, Shogo Kikuchi, Hitoshi Nakagama, and Baoen Shan

Chem. Res. Toxicol. 32 1515 - 1527 2019年06月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1021/acs.chemrestox.9b00017

Identification of the novel hcbB operon catalyzing the dechlorination of pentachlorophenol in the Gram-positive bacterium Nocardioides sp. strain PD653 査読あり国際誌

Koji Ito, Kazuhiro Takagi, Yoshitaka Matsushima, Akio Iwasaki, Naoto Tanaka, Yu Kanesaki, Fabrice Martin-Laurent, Shizunobu Igimi

J. Pestic. Sci. 43 ( 2 ) 124 - 131 2018年04月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1584/jpestics.D17-089

Short synthesis of epi-cytoxazone via oxazoline formation through intramolecular benzylic substitution of a bis-trichloroacetimidate 査読あり国際誌

Yoshitaka Matsushima, Moe Ishikawa, Rina Shibasaki, Yuki Nojima

Tetrahedron Letters 59 231 - 233 2018年01月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.tetlet.2017.11.057

A versatile route to 2,4,6-trideoxy-4-aminohexoses: Stereoselective syntheses of D-vicenisamine and its epimers via iodocyclization of carbamate 査読あり国際誌

Yoshitaka Matsushima, Jun Kino

Tetrahedron 73 6831 - 6839 2017年10月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.tet.2017.10.009

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