論文 - 黒澤 亮
-
Comparison of nutritional status of domestic and nomadic goats in the suburbs of Djibouti City 査読あり 国際共著
KUROSAWA Akira, SAITO Shuhei, ADEN Dayah, KIMURA Rikako
Journal of Arid Land Studies 32 ( S ) 217 - 220 2022年12月
担当区分:筆頭著者, 責任著者 記述言語:英語 掲載種別:研究論文(国際会議プロシーディングス) 出版者・発行元:The Japanese Association for Arid Land Studies
<p>The survey was conducted to compare the nutritional status of goats owned by nomads in Djibouti with that of goats owned by settled farmers, and to gain insights into the nutritional supply of natural forage plants in Djibouti. The survey was conducted on dairy farmers in Ambuli, near the capital, and nomads grazing around Ali-Sabieh in the second city. Dairy farmers own 2-3 ha of pasture and use shallow wells and 1000 L water storage tanks as water sources. Nomads moving distance is 10 km<sup>2</sup>. In this survey, the survey items for goats were age, body length, chest circumference, body height, body weight, blood glucose level, and blood β-hydroxybutyric acid (BHBA) level. According to the results of this survey, the goats raised by nomads had a small body size and low weight for their age. However, the ratio of waist circumference to body weight was high, and it was inferred that the lumen was well developed for the physique. In addition, there was not much difference in blood glucose levels between nomadic and dairy farm animals, and it was presumed that the developing status of nomadic goats was poor. Furthermore, the BHBA levels of nomadic goats were high, suggesting that the organic acid produced in the lumen efficiently supplied energy. These results indicated that the goats bred by nomads were smaller but their nutritional status was not poorer than that of goats bred by dairy farmers.</p>
-
Educational Material Research on the Color of Crayfish for Conversion to Edible Resources
HIGUCHI SAKI, ONO HONAMI, HONDA HIROAKI, MASKEY SARVESH, KUROSAWA AKIRA, SUZUKI TOSHIHIRO, TAKAHASHI NOBUYUKI, OKAZAWA HIROMU, TAKEDA KOUJI
International Journal of Environmental and Rural Development 14 ( 1 ) 98 - 105 2023年
記述言語:英語 出版者・発行元:Institute of Environmental Rehabilitation and Conservation, Research Center
<p>The crayfish (<i>Procambarus clarkii</i>) is an invasive alien species consumed as a food source worldwide. However, there is an extremely low demand for it as a food resource in Japan, and it is typically targeted for extermination. One reason for the lack of progress in considering crayfish a sustainable food resource is limited education on the issue during secondary education programs. It is well known that astaxanthin is the pigment responsible for the body color of common red crayfish and crustaceans such as shrimp and crabs. At the same time, while there are ample study materials on plant pigments in the science education curriculum in junior high and high schools in Japan, there are no such materials to our knowledge that focus on animal pigments. Therefore, this research sought to develop teaching materials to deepen understanding of color as a food component of crayfish through an experiment involving extracting and separating their pigments. Using pigments extracted from red crayfish exoskeletons, we developed experimental teaching materials to observe astaxanthin by employing thin-layer chromatography and high-performance liquid chromatography. In addition, an experiment was conducted to observe the effect of different foods on crayfish body color. For the experiment, food was developed to make crayfish bodies white (decolorized) and orange. When the decolorized crayfish were fed norbixin or bixin, which are components of the orange annatto pigment, norbixin tended to accumulate as orange in the exoskeleton more than bixin based on the apparent body color change, color extraction from images, and thin-layer chromatography. These findings are expected to be useful for science and nutrition education, supporting the development of the students’ awareness and understanding of crayfish as a food resource.</p>
-
HIRATA Yoshiki, KATSUKURA Yusuke, HENMI Yuka, OZAWA Ren, SHIMAZAKI Sayaka, KUROSAWA Akira, TORII Yasushi, TAKAHASHI Hironori, IWATA Hisataka, KUWAYAMA Takehito, SHIRASUNA Koumei
Journal of Reproduction and Development 67 ( 4 ) 257 - 264 2021年
記述言語:英語 出版者・発行元:The Society for Reproduction and Development
<p> Advanced maternal age is a risk factor for female infertility, and placental dysfunction is considered one of the causes of pregnancy complications. We investigated the effects of advanced maternal aging on pregnancy outcomes and placental senescence. Female pregnant mice were separated into three groups: young (3 months old), middle (8–9 months old), and aged (11–13 months old). Although the body weights of young and middle dams gradually increased during pregnancy, the body weight of aged dams only increased slightly. The placental weight and resorption rate were significantly higher, and live fetal weights were reduced in a maternal age-dependent manner. Although mRNA expression of senescence regulatory factors (p16 and p21) increased in the spleen of aged dams, mRNA expression of p16 did not change and that of p21 was reduced in the placenta of aged dams. Using a cytokine array of proteins extracted from placental tissues, the expression of various types of senescence-associated secretory phenotype (SASP) factors was decreased in aged dams compared with young and middle dams. The aged maternal placenta showed reduced immune cell accumulation compared with the young placenta. Our present results suggest that models using pregnant mice older than 8 months are more suitable for verifying older human pregnancies. These findings suggest that general cellular senescence programs may not be included in the placenta and that placental functions, including SASP production and immune cell accumulation, gradually decrease in a maternal age-dependent manner, resulting in a higher rate of pregnancy complications.</p>
DOI: 10.1262/jrd.2021-034
-
SANO Michiya, SHIMAZAKI Sayaka, KANEKO Yasuaki, KARASAWA Tadayoshi, TAKAHASHI Masafumi, OHKUCHI Akihide, TAKAHASHI Hironori, KUROSAWA Akira, TORII Yasushi, IWATA Hisataka, KUWAYAMA Takehito, SHIRASUNA Koumei
Journal of Reproduction and Development 66 ( 3 ) 241 - 248 2020年
記述言語:英語 出版者・発行元:The Society for Reproduction and Development
<p> Maternal obesity is one of the major risk factors for pregnancy complications and is associated with low-grade chronic systemic inflammation due to higher levels of pro-inflammatory cytokines such as interleukin (IL)-1β. Pregnant women with obesity have abnormal lipid profiles, characterized by higher levels of free fatty acids, especially palmitic acid (PA). Previously, we reported that PA stimulated IL-1β secretion via activation of NLRP3 inflammasome in human placental cells. These observations led us to hypothesize that higher levels of PA induce NLRP3 inflammasome activation and placental inflammation, resulting in pregnancy complications. However, the effects of PA on NLRP3 inflammasome during pregnancy <i>in vivo</i> remain unclear. Therefore, PA solutions were administered intravenously into pregnant mice on day 12 of gestation. Maternal body weight was significantly decreased and absorption rates were significantly higher in PA-injected mice. The administration of PA significantly increased IL-1β protein and the mRNA expression of NLRP3 inflammasome components (<i>NLRP3</i>, <i>ASC</i>, and caspase-1) within the placenta. In murine placental cell culture, PA significantly stimulated IL-1β secretion, and this secretion was suppressed by a specific NLRP3 inhibitor (MCC950). Simultaneously, the number of macrophages/monocytes and neutrophils, together with the mRNA expression of these chemokines increased significantly in the placentas of PA-treated mice. Treatment with PA induced ASC assembling and IL-1β secretion in macrophages, and this PA-induced IL-1β secretion was significantly suppressed in NLRP3-knockdown macrophages. These results indicate that transient higher levels of PA exposure in pregnant mice activates NLRP3 inflammasome and induces placental inflammation, resulting in the incidence of absorption.</p>
DOI: 10.1262/jrd.2020-007
-
AIAを指標としたラット盲腸の食下物滞留の検討と消化試験 査読あり
池田慧,黒澤亮,池田周平,祐森誠司
関東畜産学会報 61 ( 2 ) 27 - 33 2011年04月
記述言語:日本語 掲載種別:研究論文(学術雑誌)
AIAを指標としたラットの盲腸における食下物の滞留と消化試験の予備飼育期間の検討を行った。食下物の滞留については、対照区で60時間後、盲腸切除区で36時間後にAIA排泄が安定するのを確認した。このことから、盲腸に食下物が滞留する時間は最大で24時間と考えられた。また、消化試験については、index法と全糞採取法の結果を比較したが試験法間に差はなく、このことより、AIAを指示物質としたindex法については、予備飼育期間を3日間とすることで実施可能と考えられた。
-
Investigation into the Durability of Washed Rice Straw for Equine Bedding and the Repetitive Use of Washing Water 査読あり
KUROSAWA A,D.ENDO, S.IKEDA, S.SUKEMORI
Animal Science Journal 82 ( 2 ) 334 - 339 2011年03月
担当区分:筆頭著者 記述言語:英語 掲載種別:研究論文(学術雑誌)
The present experiment investigated durability during the repetitive use of washed rice straw, as bedding material, and washing water as a model study. Residual nitrogen levels, elasticity, and water suction rates for washed rice straw-adhered urea were measured. In addition, outflow levels of nitrogen and mineral ions for washing water were measured to determine durability. In this study, it was considered that rice straw and washing water could be used repetitively three times. Furthermore, in order to maintain effluent standards, washing water from the first washing needs to be processed in a septic tank.
その他リンク: http://onlinelibrary.wiley.com/doi/10.1111/j.1740-0929.2010.00822.x/full
-
東京農業大学富士農場における飼養管理方式の変遷に伴う黒毛和種繁殖雌牛の繁殖成績
野口龍生・佐藤光夫・黒澤亮・池田周平・祐森誠司・渡邊忠男
東京農業大学農学集報 2010年06月
記述言語:日本語 掲載種別:研究論文(大学,研究機関等紀要)
本調査は、昭和62年平成18年までの間に農場で繋養してきた黒毛和種繁殖牛延べ400頭における分娩後初回人工授精に数、人工授精回数、受胎率および空胎日数について飼養管理方式の変遷と併せて解析した。その結果、昭和62年から平成13年までは舎飼主体で十分な運動が行えていないと考えられ、平成13年以降の母子分離時期の早期化と放牧面積の拡大が、受胎率向上と空胎日数短縮に良好な影響を及ぼしたと考えられた。
-
ラットの食糞行動によるビタミンB12の出納 招待あり 査読あり
黒澤亮・池田周平・澤田佳奈・山田惠子・福田守道・山田正二・祐森誠司
ビタミン 84 ( 8 ) 47 - 52 2010年02月
記述言語:日本語 掲載種別:研究論文(学術雑誌)
B12とCo含量の異なる飼料を給与した食糞行動許容と阻止ラットにおいて,Co摂取量から合成できるB12量と実際のB12排泄量の関係について検討することを目的として実施した.B12の出納については,いずれの飼料を給与したラットにおいても,排泄量が摂取量を上回り,また,食糞行動許容ラットは,阻止ラットに比べてB12排泄量が多かった.Co摂取量から合成できるB12量と実際のB12排泄量に関しては,市販配合飼料を給与したラットでは,実際に排泄したB12量は,合成可能なB12量よりも少なかったが,精製飼料を給与したラットが実際に排泄したB12量は,摂取したCo量から合成できるB12量よりも多かった.
黒澤亮・池田周平・澤田佳奈・山田惠子・福田守道・山田正二・祐森誠司 -
Seasonal Variations in L-carnitine levels in colostrum and Holstein cows
Sato M.,Kurosawa A.,Watanabe N.,Odo S.,Ikeda S.and Sukemori s.
Journal of Agriculture science, Tokyo University of Agriculture 54,214-217 2009年12月
記述言語:英語 掲載種別:研究論文(大学,研究機関等紀要)
In this study, we observed the seasonal variation in L-carnitine levels in the colostrum and milk Holstein strain cows. Milking for L-carnitine analysis was conducted on days 2, 6 and 30 after calving. In the chronological variations of L-carnitine values after calving, the highest values were recognized in the colostrum and decreased to their lowest levels in milk. The L-carinitine levels in colostrum increased mildly at the start of summer, and on day after calving at the start of autumn, it increased suddenly. The L-carnitine levels in milk remained stable. Difference in these variations in L-carnitine levels during the lactation stage appear to indicate difference in heat stress damage.
Sato M.,Kurosawa A.,Watanabe N.,Odo S.,Ikeda S.and Sukemori s. -
暑熱環境下におけるニワトリへのL-カルニチン給与が繁殖成績に及ぼす影響
佐藤光夫,黒澤亮,渡辺直久,王堂哲,池田周平,祐森誠司
関東畜産学会報 60 ( 1 ) 1 - 7 2009年10月
記述言語:日本語 掲載種別:研究論文(学術雑誌)
暑熱環境下における雌雄のニワトリに対するL-カルニチン給与が繁殖成績に及ぼす影響について検討した。50ppmのカルニチン給与は、暑熱による産卵成績の低下を改善する可能性が示唆された。受精卵の発生率、孵化率については有意の差は認められなかった。また、血漿中カルニチン濃度については個体差が大きく給与の影響は明らかにならなかった。
-
ブロイラーの成長に対する有胞子性乳酸菌およびガラクトオリゴ糖添加効果の検討
黒澤亮・東修平・熊谷秀俊・池田周平・祐森誠司
関東畜産学会 59 ( 2 ) 78 - 82 2009年03月
担当区分:筆頭著者 記述言語:日本語 掲載種別:研究論文(学術雑誌)
本研究は,有胞子性乳酸菌(Bacillus coagulans)(以降乳酸菌)とガラクトオリゴ糖を飼料に添加し,これらがブロイラーの成長に及ぼす影響を検討する目的で行った。ブロイラーの増体量は,乳酸菌区が対照区に比べて有意(P<0.05)に高い値を示した。ガラクトオリゴ糖区は対照区に比べて増体量が高くなる傾向を示したが,乳酸菌+ガラクトオリゴ糖区では,複合的効果は認められなかった。ブロイラーの飼料摂取量は乳酸菌区が最も多く,乳酸菌+ガラクトオリゴ糖区,ガラクトオリゴ糖区,対照区の順となった。したがって,乳酸菌の飼料添加は,飼料摂取量の増加ならびにブロイラーの摂取した栄養素の利用性を向上させた結果,有意な増体を引き起こしたものと考えられた。
黒澤亮・東修平・熊谷秀俊・池田周平・祐森誠司 -
島根県下で有害駆除されたイノシシの背脂肪融点および脂肪酸組成
庵屋敷瑛子・澤田誠吾・山川渉・黒澤亮・鈴木敏郎・池田周平・祐森誠司
関東畜産学会 59 ( 2 ) 71 - 77 2009年03月
記述言語:日本語 掲載種別:研究論文(学術雑誌)
島根県で有害駆除されたイノシシ肉の有効利用の指標として背脂肪の脂肪酸組成と融点の通年変化を検討した。融点は夏季に有意に高い値を示した(P<0.05)。背脂肪の脂肪酸組成割合は内層と外層で同様の変動傾向を示したが、脂肪酸割合の季節変化は異なり、飽和脂肪酸は秋季に多く、春季に少ない傾向を示し、リノール酸は逆の変動傾向を示した。また、オレイン酸は春季から夏季に多く、リノレン酸は秋季から冬季に減少した。駆除個体を特産物として利用するには早秋からの狩猟が好ましいと考えられた。
-
Effect of L-carnitine supplementation on growth performance of beef calves
M.Sato, A.Kuroswa, S.Ikeda, N.Watanbe, S.Odo, and S.Sukemori
Journal of Agriculture science, Tokyo University of Agriculture, 53(4), 317-321, 2009 2009年03月
記述言語:英語 掲載種別:研究論文(学術雑誌)
The present experiment aimed to estimate the effect of dietary supplementation of L-carnitine to calves on their growth performance. Determination of body weight and body measurement (withers height, body length, Chest depth, chest girth and height at hip cross) was conducted every week. The present results suggested that L-carnitine supplemantation to the milk replacer supported the growth performance of calves.
M.Sato, A.Kuroswa, S.Ikeda, N.Watanbe, S.Odo, and S.Sukemori -
ハーブ精油によるハエ忌避効果の検討
佐藤光夫・黒澤亮・池田周平・祐森誠司
日本畜産環境学会 7 ( 1 ) 35 - 40 2008年08月
記述言語:日本語 掲載種別:研究論文(学術雑誌)
本試験は、牛舎内でウシに飛来するハエに対するハーブ精油(ペパーミント、バジル、レモングラス)の忌避効果を検討することを目的に水槽の中での模擬試験と実際の牛舎での試験を実施した。閉鎖系である水槽の試験に比べて忌避効果が低下するまでの時間は短かったが、4000倍希釈したペパーミント精油の噴霧により、ハエ飛来は抑制され牛へのストレスの低減が期待できると考えられた。また、8000倍希釈液の噴霧においても、搾乳作業時間中のハエの飛来を抑制することに有効な手段であると考えられた。
-
Response of plasma free lysine and valine concentration of growing rabbits to dietary changes in both amino acid levels
H. Muramatu, T. Erikawa, M. Takeishi, Y. Takahashi, A. Kurosawa, S. Ikeda, H. Shibui, S. Sukemori
Journal of Pet Animal Nutrition, 11(2): 61-66, 2008 2008年
記述言語:英語 掲載種別:研究論文(学術雑誌)
In order to predict lysine and valine requirements in rabbits using the response of plasma free amino acid concentrations to the amounts of both amino acids in diets. The lysine reqirement at 12% CP was obtained as an intersection between the basal line and increasing line, leading to the value of 0.63%. Because plasma lysine at 16 to 20% CP levels as plasma valine at all CP levels increased lineary without the basal line, reqirements of lysine and valine were not clear within the range of these CP Levels.
H. Muramatu, T. Erikawa, M. Takeishi, Y. Takahashi, A. Kurosawa, S. Ikeda, H. Shibui, S. Sukemori -
Effect of L-Carnitine supplementation to bulls on sperm motility maintenance under high environmental temperatures
M.Sato, A.Kuroswa, S.Ikeda, N.Watanbe, S.Odo, Y.Monji and S.Sukemori
Animal Behaviour and Management, 2008 44 ( 4 ) 245 - 250 2008年
記述言語:英語 掲載種別:研究論文(学術雑誌)
This study aimed to evaluate some effects of L-carnitine supplementation to bull on its sperm motility under high environmental temperature. Present results suggested that the supplementation of L-carnitine recover a course of energy metabolism reduced by the high environmental temperature. L-carnitine supplementation halted the reduction of sperm motility due to heat stress.
M.Sato, A.Kuroswa, S.Ikeda, N.Watanbe, S.Odo, Y.Monji and S.Sukemori -
Effects of cecectomy on digestion and retention time of digesta in rats 査読あり
黒澤亮、池田周平、萩原友子、 祐森誠司、 栗原良雄
Animal Science Journal 78(1)pp.47-54 2007年02月
担当区分:筆頭著者 記述言語:英語 掲載種別:研究論文(学術雑誌)
盲腸の役割について検討する目的で盲腸切除ラットの成長、消化率、消化管滞留時間について経時的に検討した。増体量、消化率、消化管滞留時間に対する盲腸切除の影響は経時的に変化し、盲腸の役割は成長ステージによって変化するものと考えられた。
-
ラットのビタミンB12合成とコバルトの出納に関する検討
黒澤亮、池田周平、栗原良雄、 祐森誠司
関東畜産学会報 57 ( 1 ) 1 - 5 2007年02月
担当区分:筆頭著者 記述言語:日本語 掲載種別:研究論文(学術雑誌)
本試験は、ラットの含水分に多く含まれるビタミンB12について、構成成分であるコバルトを指標に、その出納について検討した。ビタミンB12は微生物定量で測定し、コバルトは原子吸光法で測定した。試験結果において腸管内のビタミンB12合成はコバルトの動態と一致しないことが認められたがその理由については明らかにならなかった。
-
ラットの食糞行動と栄養素摂取の関係 査読あり
祐森誠司、黒澤亮、池田周平、栗原良雄
ビタミン学会誌 80 ( 9 ) 457 - 464 2006年09月
担当区分:筆頭著者 記述言語:日本語 掲載種別:研究論文(学術雑誌)
含水糞は、普通糞に比べてアミノ酸、ミネラル、ビタミンを多く含む。特にVB12は、飼料から摂取する約5倍量が含水糞から供給される。しかし、盲腸切除ラットは、VB12の供給が不十分であるが通常の成長を示すことや、コバルトの摂取量からは合成が不可能な量のVB12が体内で合成されていることについては今後の検討課題である。
-
Investigationon the growth of coprophagy-prevented rats with supplemented vitaminB12
祐森誠司、黒澤亮、池田周平、栗原良雄
Journal of Animal Physiology and Animal Nutrition 90 pp.402-406 2006年08月
担当区分:筆頭著者 記述言語:英語 掲載種別:研究論文(学術雑誌)
ラットは食糞行動の阻止により飼料摂取量が減少し成長が遅延する。食糞行動阻止ラットに対して含水糞と同等のビタミンB12を投与、また飼料中にVB12量を100,1000倍に調整した際の成長について検討した。その結果、VB12の投与は食糞行動阻止による影響を改善しなかった。