Updated on 2023/07/06



Associate Professor


Associate Professor

Laboratory Address


External Link

Degree 【 display / non-display

  • 博士(農学) ( 2020.10   東京農業大学 )

  • 学士(獣医学) ( 1992.03   酪農学園大学 )

Employment Record in Research 【 display / non-display

  • 東京農業大学   農学部富士農場   准教授


Professional Memberships 【 display / non-display

  • 日本胚移植技術研究会


  • 薬事・食品衛生審議会専門委員会

    2015.01 - 2017.01

Research Areas 【 display / non-display

  • Life Science / Veterinary medical science

  • Life Science / Animal production science

Research Interests 【 display / non-display

  • 受胎性向上

  • 家畜衛生

  • 生産性向上

  • ウシ胚移植

Papers 【 display / non-display

  • Cell free mitochondrial DNA in plasma and physical condition of cows Reviewed

    NOGUCHI Tatsuo, AIZAWA Takuro, IWATA Hisataka

    Nihon Chikusan Gakkaiho   91 ( 3 )   227 - 232   2020.08

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    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japanese Society of Animal Science  

    <p>Blood contains cell free DNA (cfDNA), and cell free mitochondrial DNA(cf-mtDNA) is able to be easily detected by real time PCR. The present study investigated the relationship between the amount of cf-mtDNA in plasma and physiological conditions of cows. In addition, relationship between preg-nancy following artificial insemination and concentration of cf-mtDNA in the corresponding plasma. Significantly positive relationship was found between concentration of LDH and cf-mtDNA, and negative relationship was found between concentration of triglyceride and cf-mtDNA. There was no significant difference in the amount of cf-mtDNA between pregnant or non-pregnant cows following artificial insemination.</p>

    DOI: 10.2508/chikusan.91.227

    CiNii Articles

  • Comparison of gene expression and mitochondria number between bovine blastocysts obtained <i>in vitro</i> and <i>in vivo</i> Reviewed

    NOGUCHI Tatsuo, AIZAWA Takuro, MUNAKATA Yasuhisa, IWATA Hisataka

    Journal of Reproduction and Development   66 ( 1 )   35 - 39   2020

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Society for Reproduction and Development  

    <p> Embryo transfer uses embryos developed <i>in vivo</i> or <i>in vitro</i> for cattle production, however there is a difference in the quality of the embryos obtained by the two methods. This study addresses the differences in gene expression between blastocysts developed <i>in vitro</i> and <i>in vivo</i>. <i>In vivo</i> blastocysts were flushed from the uteri of super-ovulated cows and blastocysts developed <i>in vitro</i> were derived from <i>in vitro</i> matured and fertilized embryos. The same batch of frozen bull sperm was used for insemination and <i>in vitro</i> fertilization. Blastocysts were then subjected to RNA sequencing. Differentially expressed genes upregulated in <i>in vitro</i> blastocysts were annotated to focal adhesion, extracellular matrix (ECM)-receptor interaction, and PI3K-Akt signaling and the genes that were upregulated in <i>in vivo</i> blastocysts were annotated to oxidation-reduction processes, mitochondrion organization, and mitochondrial translation. Although the total cell number of the two types of blastocysts was similar, the mitochondrial quantity (determined by mitochondrial DNA copy numbers and expression levels of TOMM20), and ATP content in the blastocysts were lower in <i>in vivo</i> blastocysts compared with those developed <i>in vitro</i>. In conclusion, RNAseq revealed differential molecular backgrounds between <i>in vitro</i> and <i>in vivo</i> developed blastocysts and mitochondrial number and function are responsible for these differences.</p>

    DOI: 10.1262/jrd.2019-100


    CiNii Articles

  • Predicting of molecules mediating an interaction between bovine embryos and uterine epithelial cells Reviewed

    NOGUCHI Tatsuo, HAYASHI Takeshi, INOUE Yuki, HARA Shunsuke, SHIRASUNA Koumei, IWATA Hisataka

    Journal of Reproduction and Development   68 ( 5 )   318 - 323   2022.10

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Society for Reproduction and Development  

    <p> Embryo-maternal reproductive tract interactions are pivotal for successful pregnancy. The present study predicted the molecules modulating embryo-uterine communication by comparing two sets of differentially expressed genes (DEGs): DEGs in uterine epithelial cells (UECs) collected from the uterus with and without blastocysts and DEGs between blastocysts developed <i>in vivo</i> and <i>in vitro</i>. Cows were subjected to super ovulation (SOV), followed by insemination or non-insemination at estrus (SOV + AI and SOV cows). Seven days after estrus, the uterus was flushed to collect UECs, and the presence of blastocysts in the uterus was confirmed. UECs were subjected to RNA-Sequencing (RNA-Seq) to identify DEGs. Publicly available RNA-Seq data of <i>in vivo</i> and <i>in vitro</i> developed bovine blastocysts were used to determine DEGs. Then, using ingenuity pathway analysis, activated- and inhibited-upstream regulators (USRs) for UECs in blastocysts were compared with those for blastocysts developed <i>in vivo</i>. RNA-Seq of UECs revealed that the DEGs were associated with immune response and cell adhesion pathways. The activated and inhibited USRs of UECs derived from SOV+ AI cows overlapped with the activated and inhibited USRs of blastocysts developed <i>in vivo</i>. Overlapping activated USRs include leukemia inhibitory factor, interleukin 6, fibroblast growth factor-2, transforming growth factor beta-1, and epidermal growth factor. In conclusion, the present study predicted the molecules that potentially mediate communication between the developing embryo and the uterus <i>in vivo</i> and prepare the uterus for pregnancy.</p>

    DOI: 10.1262/jrd.2022-046


  • Molecular analysis of bovine leukemia virus in early epidemic phase in Japan using archived formalin fixed paraffin embedded histopathological specimens Reviewed

    NISHIKAKU Kohei, NOGUCHI Tatsuo, MURAKAMI Satoshi, TORII Yasushi, KOBAYASHI Tomoko

    Journal of Veterinary Medical Science   84 ( 3 )   350 - 357   2022

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOCIETY OF VETERINARY SCIENCE  

    <p>Bovine leukemia virus (BLV) is an important pathogen associated with enzootic bovine leukosis. In this study, we performed PCR and sequencing analysis to characterize BLVgp51 sequences from formalin-fixed paraffin-embedded (FFPE) specimens made from 1974 to 2000 and successfully obtained BLV proviral genome sequences from 94% of the analyzed samples. Furthermore, from these samples, we reconstructed eight full-length and nearly full-length BLVgp51 sequences. These sequences were classified as BLV genotype 1, implying that genotype1 has already been circulating in Japan since the 1970s. In our results, the proviral DNA was detected in the 1970s, 1980s, and 1990s in the same manner, indicating that the detection of BLV proviral genome depends on storage conditions rather than storage period. The sequences obtained in this study provide direct insights into BLV sequences before 2000, which serves as a good calibrator for inferring ancient BLV diversity.</p>

    DOI: 10.1292/jvms.21-0570


    CiNii Articles

  • 褐毛和種におけるBoLA-DRB3遺伝子の多様性解析 Reviewed

    西角光平, 稲永敏明, 西明仁, 米澤隆弘, 野口龍生, 鳥居恭司, 今川和彦, 小林朋子

    獣医疫学雑誌   26 ( 2 )   2022

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

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Books and Other Publications 【 display / non-display

  • 家畜人工授精師講習会テキスト(家畜体内受精卵・家畜体外受精卵移植編)別冊

    野口龍生、轟木淳一、濱野晴三、中原仁、黒沢功、森安悟、上田淳一、木村博久( Role: Joint author ,  P27~32、P46~48、P70~71)

    社団法人家畜改良事業団、社団法人日本家畜人工授精師協会  2010.03 

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  • 東北地域 新しい技術シリ-ズ 平成14年度

    野口龍生 他( Role: Joint author ,  p63)

    東北農業研究センター  2003.10 

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Misc 【 display / non-display

  • 成長促進目的での抗菌剤は本当に必要なのか? Invited

    野口 龍生

    臨床獣医   2020.07

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

  • 農家における牛の発情発見指標評価


    家畜衛生年報 (電子冊子)   2008.04

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (other)   Publisher:平成19年度岩手県  


  • 牛の過剰排卵処理における施灸効果


    会報 通刊24号    38 - 39   2008.01

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (other)   Publisher:東日本家畜受精卵移植技術研究会  


  • 乳牛の効率的な産次間採胚に向けた基礎調査


    年報 (電子冊子)   2007.04

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (other)   Publisher:平成18年度岩手県家畜衛生  


  • 乳牛の効率的な産次間採胚に向けた基礎調査


    会報 通刊23号    22 - 23   2006.01

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (other)   Publisher:東日本家畜受精卵移植技術研究会  


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Past of Commissioned Research 【 display / non-display

  • 過酸化物還元能力を有する乳酸菌の豚への投与効果に関する研究

    2011.10 - 2012.09

    大洋香料株式会社  一般受託研究  The General Consignment Study

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Presentations 【 display / non-display

  • 乳酸菌投与が子豚期の発育ならびに肥育成績に及ぼす影響


    第95回日本養豚学会大会  2011.10  日本養豚学会

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    Event date: 2011.10

    Language:Japanese   Presentation type:Oral presentation (general)  



  • Differences of developmental ability and methylation pattern between the NT embryos from bovine ivf ES cells and nt ES cells.


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    Event date: 2011.09

    Language:Japanese   Presentation type:Poster presentation  

  • 黒毛和種繁殖雌牛におけるアスタキサンチンの吸収について International conference


    第7回アスタキサンチン研究会  2011.09  アスタキサンチン研究会

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    Event date: 2011.09

    Language:Japanese   Presentation type:Oral presentation (general)  



  • Palatability of pellets made from green ta leaf by-products as feedstuff for pigs International conference


    The 4th congress of Asian pig veterinary society (in Tsukuba)  2009.10 

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    Event date: 2009.10

    Language:English   Presentation type:Oral presentation (general)  


  • 寒冷下哺乳子牛のエネルギー補給 International conference


    日本畜産学会第111回大(於 琉球大学)  2009.09 

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    Event date: 2009.09

    Language:Japanese   Presentation type:Oral presentation (general)  


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Teaching Experience 【 display / non-display

  • 農業専門実習

  • 家畜品種論

  • 家畜論(牛)

  • 畜産実習概論

  • 人工授精

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Committee Memberships 【 display / non-display

  • 日本胚移植技術研究会   常任理事  


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    Committee type:Academic society

  • 日本獣医学会   評議委員  


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    Committee type:Academic society

  • 薬事・食品衛生審議会専門委員会   動物用一般医薬品調査会員  

    2015.01 - 2017.01   

Social Activities 【 display / non-display

  • パキスタン国ハイバル・パフトゥンハー州畜産開発を通じた生計向上プロジェクト

    Role(s): Consultant

    JICA  2022.02

  • 静岡県畜産技術研究所研究アドバイザー

    Role(s): Consultant

    2012.06 - 2013.03

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    Type:Research consultation


Attractiveness of Research 【 display / non-display

  • よく学び、よく遊ぶ、Vitality溢れる方をお待ちしています