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Naoto Tanaka Professor |
From Graduate School 【 display / non-display 】
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Tokyo University of Agriculture Doctoral program (second term) Completed
1998.04 - 2001.03
Country：Japan
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Tokyo University of Agriculture Doctoral program (first term) Completed
1996.04 - 1998.03
Country：Japan
Degree 【 display / non-display 】
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博士（農芸化学） ( 2001.03 東京農業大学 )
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修士（農芸化学） ( 1998.03 東京農業大学 )
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Applied Bio-Science Department of Applied Biology and Chemistry
2001.04 - 2001.06
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Tokyo University of Agriculture Faculty of Applied Bio-Science Culture Collection Center Lecturer
2007.04 - 2010.09
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Tokyo University of Agriculture Graduate School of Agriculture Department of Environmental Symbiotic Studies Lecturer
2009.04 - 2010.09
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Tokyo University of Agriculture Graduate School of Agriculture Department of Environmental Symbiotic Studies Associate Professor
2010.10 - 2013.03
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Tokyo University of Agriculture Faculty of Applied Bio-Science Associate Professor
2010.10 - 2015.03
Papers 【 display / non-display 】
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Analyses of hyphal diversity in Trichosporonales yeasts based on fluorescent microscopic observations Reviewed International journal
Keita Aoki, Moriya Ohkuma, Takashi Sugita, Yuuki Kobayashi, Naoto Tanaka, Masako Takashima
Microbiology Spectrum 2025.02
Language：English Publishing type：Research paper (scientific journal)
In dimorphic yeasts, hyphal growth is primarily associated with infection and mycosis progression, with Trichosporon asahii causing deep-seated mycosis and summer-type hypersensitivity pneumonitis. Magnesium accelerates hyphal growth in T. asahii, leading to multi-septation, vacuolar expansion, and decreased lipid droplet size. However, the commonality of these phenotypes has not been studied in Trichosporonales yeasts. Therefore, to explore whether similar magnesium-induced phenotypes occur across Trichosporonales yeasts, we examined hyphal growth, multi-septation, vacuolar extension, and lipid droplet size and number in 30 species. Cell length increased with magnesium treatment in 13 yeasts: 5 Trichosporon (T. asahii, Trichosporon aquatile, Trichosporon asteroides, Trichosporon coremiiforme, and Trichosporon ovoides), three Apiotrichum (Apiotrichum brassicae, Apiotrichum montevideense, and Apiotrichum veenhuisii), three Cutaneotrichosporon (Cutaneotrichosporon cavernicola, Cutaneotrichosporon cutaneum, and Cutaneotrichosporon dermatis), Pascua guehoae, and Takashimella koratensis. C. dermatis also underwent pseudo-hyphal growth. Multi-septation increased in seven dimorphic yeasts, including five Trichosporon spp., Trichosporon faecale, and C. dermatis. The vacuolar area was significantly extended in T. asahii, T. aquatile, T. ovoides, and C. dermatis. Lipid droplet size increased only in Trichosporon inkin; however, it decreased in T. asahii, T. coremiiforme, and T. faecale. Additionally, lipid droplet number was preferentially altered in Apiotrichum and Cutaneotrichosporon. These results suggested that magnesium-induced multi-septation and vacuolar area expansion phenotypically distinguish Trichosporon hyphae from Apiotrichum and Cutaneotrichosporon hyphae and distinguish C. dermatis pseudo-hyphae from Cutaneotrichosporon spp. Moreover, differences in lipid droplet size can discriminate species within Trichosporon. Our results suggest that phenotypic alteration via magnesium treatment can contribute to the characterization of Trichosporonales yeasts. These findings provide insights into fungal pathogenesis and may support new treatment strategies.IMPORTANCEMagnesium sulfate considerably affects hyphal growth and cellular organization in Trichosporon asahii. To examine the commonality of this phenotype in Trichosporonales, we treated 30 Trichosporonales yeasts with magnesium sulfate and observed genus-level phenotypic alterations. Using cell length measurement, lipid droplet staining, septum staining, and vacuole staining, considerable hyphal diversity was observed among Trichosporonales. Notably, differences in the multi-septation phenotype and vacuolar size distinguished Trichosporon hyphae from Apiotrichum and Cutaneotrichosporon hyphae and distinguished Cutaneotrichosporon dermatis from other Cutaneotrichosporon spp. Moreover, differences in lipid droplet phenotype divided Trichosporon hyphae into two groups. Our study revealed the relationship between hyphal morphology and phylogenetic systematics in Trichosporonales.
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Antimicrobial activity, genome analysis, and gene cluster encoding the plantaricin production of Lactiplantibacillus plantarum ZBK1-5 Reviewed International coauthorship International journal
N. Choovet, T. Jirakanjanasit, P. Subphon, S. Thepsombat, E. Kingkaew, N. Tanaka, Y. Shiwa, S. Tanasupawat, S. Phuengjayaem
ScienceAsia 50 ( 1 ) 1 - 12 2024.05
Language：English Publishing type：Research paper (scientific journal) Publisher：Science Society of Thailand
This study aimed to explore bacteriocin-producing lactic acid bacteria (LAB), identify the genes influencing bacteriocin synthesis, and assess their probiotic potential via safety evaluations both in vitro and in silico. The strain ZBK1-5, isolated from pickled ginger, was identified as Lactiplantibacillus plantarum. The bacteriocin ZBK1-5 exhibited the highest antimicrobial activity with 6,400 AU/ml at 18 h, predominantly effective against Gram-positive bacteria and stable within pH 2?9. Significantly, the genome analysis confirmed the existence of bacteriocin synthesis genes, specifically plantaricin KJ, EF, A, and N. Simultaneously, the safety evaluations showed its low risk of antibiotic resistance gene transfer, thereby emphasizing the safety profile of the strain ZBK1-5. The genetic components associated with the ability of L. plantarum ZBK1-5 to survive and adapt in gastrointestinal conditions provide additional evidence (16 genes related to acid stress and 9 genes associated with bile resistance), supporting its potential as a probiotic candidate. These findings were validated through in vitro digestion conditions, where the viability of the cells was observed to be 68.26, 66.62, and 60.91% during transit through the oral, gastrointestinal, and small intestinal phases, respectively. Additionally, the strain ZBK1-5 showed a 90.39% adhesion rate in the Caco-2 cell line. The unique characteristics of plantaricin produced by L. plantarum ZBK1-5 exhibit potent antibacterial activity, rapid time to production compared to other known plantaricins, and strain safety for application and upscaling. This research study offers significant scientific insights into L. plantarum ZBK1-5, a plantaricin producer, emphasizing its promising potential for future applications.
DOI： 10.2306/scienceasia1513-1874.2024.046
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SY7-1 蛍光顕微鏡観察によって明らかになるトリコスポロン酵母の性状 Reviewed International journal
青木 敬太, 大熊 盛也, 杉田 隆, 小林 裕樹, 田中 尚人, 高島 昌子
日本医真菌学会総会プログラム・抄録集 65.Suppl1 ( 0 ) 86 2024
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：日本医真菌学会
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Chromosome-level genome assemblies of Cutaneotrichosporon spp. (Trichosporonales, Basidiomycota) reveal imbalanced evolution between nucleotide sequences and chromosome synteny Reviewed International journal
Yuuki Kobayashi, Ayane Kayamori, Keita Aoki, Yuh Shiwa, Minenosuke Matsutani, Nobuyuki Fujita, Takashi Sugita, Wataru Iwasaki, Naoto Tanaka & Masako Takashima
BMC Genomics 24 609 2023.10
Language：English Publishing type：Research paper (scientific journal)
Since DNA information was first used in taxonomy, barcode sequences such as the internal transcribed spacer (ITS) region have greatly aided fungal identification; however, a barcode sequence alone is often insufficient. Thus, multi-gene- or whole-genome-based methods were developed. We previously isolated Basidiomycota yeasts classified in the Trichosporonales. Some strains were described as Cutaneotrichosporon cavernicola and C. spelunceum, whereas strain HIS471 remained unidentified. We analysed the genomes of these strains to elucidate their taxonomic relationship and genetic diversity.
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Characterization and genomic analysis of Fererhizobium litorale gen. nov., sp. nov., isolated from the sandy sediments of the Sea of Japan seashore. Reviewed International coauthorship International journal
Lyudmila Romanenko, Nadezhda Otstavnykh, Naoto Tanaka, Valeriya Kurilenko, Vasily Svetashev, Liudmila Tekutyeva, Valery Mikhailov, Marina Isaeva
Microorganisms 11 2385 2023.09
Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI journals
Books and Other Publications 【 display / non-display 】
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田中尚人（ Role： Joint author , 東京農業大学の乳酸菌コレクション）
(株)エヌ・ティー・エス 2023.09 （ ISBN:978-4-86043-858-6 ）
Language：Japanese Book type：General book, introductory book for general audience
生物資源として、生きた研究材料として重要性が高まる中、研究の発展に寄与する微生物資源の利活用について解説している。また、微生物メタゲノム情報の解析や環境サンプルの直接研究により明らかになりつつ微生物の多様性・生態について記載している。
Misc 【 display / non-display 】
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公益財団法人発酵研究所 学会・研究部会助成 駒形和男先生追悼公開シンポジウム 「新たな時代を拓く微生物分類学：現状と展望」
田中 尚人, 飯野 隆夫, 石田 達也, 伊藤 隆, 乙黒 美彩, 河地 正伸, 鈴木 誠, 高島 昌子
日本微生物資源学会誌 39 ( 1 ) 41 - 45 2023
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Meeting report Publisher：日本微生物資源学会
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乳酸菌を分離するための基本 Invited Reviewed
田中尚人
日本乳酸菌学会誌 30 ( 1 ) 3 - 7 2019.03
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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ユーザーの求める機能性乳酸菌株のオンライン選抜システムの開発
田中尚人，宮崎智，鈴木智典，冨田理，梶川揚申，内野昌孝，五十君靜信，岡田早苗
日本微生物資源学会誌 33 ( 1 ) 31 - 37 2017.06
Authorship：Lead author Language：English Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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デルブレッキー菌は変な乳酸菌
田中尚人
生物工学会会誌 95 ( 4 ) 207 2017.04
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)
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これからのハラール研究
阿部尚樹、内野昌孝、野口治子、田中尚人、岡大貴
食品機械装置 2016.05
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
Past of Cooperative Research 【 display / non-display 】
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発酵食品由来微生物の分類学的研究
2014.09 - 2017.03
独立行政法人製品評価技術基盤機構 国内共同研究 Cooperative Research within Japan
国内外発酵食品由来乳酸菌の系統分布の解析および新種提唱
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新規機能性乳酸菌資源開発の共同事業
2011.08 - 2014.03
独立行政法人製品評価技術基盤機構 国内共同研究 Cooperative Research within Japan
モンゴルの乳酸菌の分離及び系統解析による系統的分布と応用利用のための性状解析
Presentations 【 display / non-display 】
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Comparative genomics for reclassification of Stenotrophomonas maltophilia reference strains and biovar II group International conference
2022.03
Event date： 2022.03
Language：Japanese Presentation type：Oral presentation (general)
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Characterization of biofilm formation and constituent bacterial species by addition of siderophores to environmental samples International conference
2022.03
Event date： 2022.03
Language：Japanese Presentation type：Oral presentation (general)
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Analysis of mating-type loci of Cutaneotrichosporon cavernicola strains by comparative genomics International conference
2022.03
Event date： 2022.03
Language：Japanese Presentation type：Oral presentation (general)
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Effects of Lactobacillus paracasei K71 on gut microbiota composition in ob/ob mice International conference
2019.03
Event date： 2019.03
Language：Japanese Presentation type：Oral presentation (general)
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Effects of biodegradable plastic on river microbiome International conference
2019.03
Event date： 2019.03
Language：Japanese Presentation type：Oral presentation (general)
Committee Memberships 【 display / non-display 】
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日本微生物資源学会 理事（会計）
2014.09
Committee type：Academic society
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日本微生物資源学会 理事（編集副委員長）
2009.04 - 2014.08
Committee type：Academic society
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日本微生物系統分類研究会 理事（会計）
2008.01 - 2015.03