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YOKOTA Kenji Professor |
From Graduate School 【 display / non-display 】
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Tohoku University Graduate School, Division of Agriculture Doctoral program (second term) Completed
1996.04 - 2000.03
Country：Japan
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Tokyo University of Agriculture Graduate School, Division of Agriculture Master Course Completed
1994.04 - 1996.03
Country：Japan
Studying abroad experiences 【 display / non-display 】
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2012.04 - 2013.03 University of California, Berkeley Visiting scholar
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Applied Bio-Science Department of Applied Biology and Chemistry Research Assistant
2001.04 - 2007.03
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Tokyo University of Agriculture Faculty of Applied Bio-Science Department of Applied Biology and Chemistry Assistant Professor
2007.04 - 2012.09
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Tokyo University of Agriculture Faculty of Applied Bio-Science Department of Applied Biology and Chemistry Associate Professor
2012.10 - 2018.03
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Tokyo University of Agriculture Faculty of Applied Bio-Science Department of Agricultural Chemistry Associate Professor
2018.04 - 2019.03
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Tokyo University of Agriculture Faculty of Applied Bio-Science Department of Agricultural Chemistry Professor
2019.04
External Career 【 display / non-display 】
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東北大学大学院 農学研究科農芸化学専攻 日本学術振興会特別研究員
2000.04 - 2001.03
Country：Japan
Research Areas 【 display / non-display 】
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Life Science / Applied microbiology
Papers 【 display / non-display 】
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TAMAKI Mizuna, CHIKU Tsutomu, SUZUKI Shunya, MISAKI Aya, FUNAKUBO Asuka, MATSUSHIMA Yoshitaka, YOKOTA Kenji, IGIMI Shizunobu, KAJIKAWA Akinobu
Bioscience of Microbiota, Food and Health 44 ( 1 ) 70 - 79 2024.09
Language：English Publisher：BMFH Press
Although the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas system has been extensively developed since its discovery for eukaryotic and prokaryotic genome editing and other genetic manipulations, there are still areas warranting improvement, especially regarding bacteria. In this study, BRD0539, a small-molecule inhibitor of Streptococcus pyogenes Cas9 (SpCas9), was used to suppress the activity of the nuclease during genetic modification of Lacticaseibacillus paracasei, as well as to regulate CRISPR interference (CRISPRi). First, we developed and validated a CRISPR-SpCas9 system targeting the sirA gene of L. paracasei. Then BRD0539 was used for CRISPR-dependent DNA cleavage in vivo. Our results suggested that the inhibitor worked partially in both Escherichia coli and L. paracasei. Next, we designed a CRISPRi system in a L. paracasei strain by inserting an inactive SpCas9 gene into the chromosome and introducing a plasmid encoding for a single guide RNA (sgRNA) targeting the sirA gene. Expression of sirA was successfully inhibited in the recombinant strains, and CRISPRi was abolished in an inhibitor-dependent manner. Our findings may help expand the CRISPR toolbox for research on lactic acid bacteria and other microbes.
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Heat-stable spores of carotenoid-producing Bacillus marisflavi and non-pigmented Bacillus subtilis cooperatively promote growth, quality, and gut microbiota of white-leg shrimp Reviewed International coauthorship International journal
T.T. Nguyen, A.T.P. Bui, N.T.H. Le, H.T. N. Vo, A.H. Nguyen, T.D. Pham, T. Hara, K. Yokota, M. Matsutani, Y. Takatsuka, A.T.V. Nguyen
Beneficial Microbes 2023.11
Language：English Publishing type：Research paper (scientific journal)
We evaluated the benefits of heat-stable carotenoid-producing Bacillus marisflavi SH8 spores individually and in combination with non-pigmented Bacillus subtilis SH23 spores on growth, colour change, nutritional content, innate immunity, and gut microbiota of white-leg shrimp. White-leg shrimp (Litopenaeus vannamei; n = 30 per tank; 2 tanks per group) were provided feed without (control group) or with SH8, SH23, or mixed spores (total, 1×106 cfu/g pellet) for 28 d. The SH8 and SH8-23 combination groups had significantly higher specific growth rates (9.6 and 11.0%), improved red-colour score (4 scores), astaxanthin concentration (1.8- and 2.3-fold), lipid contents (30 and 50%), and superoxidase dismutase activity (8.5 and 12.3%) than that of the control group. Analysis of shrimp’s gut microbiome using 16S rRNA metagenome sequencing revealed increased abundance of four useful species and reduced abundance of four harmful species in the combination group than in the control group. Heat-stable Bacillus spore combination improved growth parameters, nutrient content, red-colour score, live counts, and abundance of useful bacteria in the gut of L. vannamei. This is the first study to show the benefits of combining highly heat-stable pigmented and non-pigmented Bacillus spores and their possible mechanisms in a shrimp model.
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Negative chemotaxis of Ligilactobacillus agilis BKN88 against gut-derived substances Reviewed International journal
Shunya Suzuki, Kenji Yokota, Shizunobu Igimi, Akinobu Kajikawa
Scientific Reports 13 2023.09
Language：English Publishing type：Research paper (scientific journal)
Ligilactobacillus agilis is a motile lactic acid bacterium found in the gastrointestinal tracts of animals. The findings of our previous study suggest that the motility of L. agilis BKN88 enables gut colonization in murine models. However, the chemotactic abilities of motile lactobacilli remain unknown. This study aimed to identify the gut-derived chemoeffectors and their corresponding chemoreceptors in L. agilis BKN88. Chemotaxis assays with chemotactic and non-chemotactic (ΔcheA) L. agilis strains revealed that low pH, organic acids, and bile salts served as repellents. L. agilis BKN88 was more sensitive to bile and acid than the gut-derived non-motile lactobacilli, implying that L. agilis might utilize motility and chemotaxis instead of exhibiting stress tolerance/resistance. L. agilis BKN88 contains five putative chemoreceptor genes (mcp1–mcp5). Chemotaxis assays using a series of chemoreceptor mutants revealed that each of the five chemoreceptors could sense multiple chemoeffectors and that these chemoreceptors were functionally redundant. Mcp2 and Mcp3 sensed all tested chemoeffectors. This study provides further insights into the interactions between chemoreceptors and ligands of motile lactobacilli and the unique ecological and evolutionary features of motile lactobacilli, which may be distinct from those of non-motile lactobacilli.
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エノキタケ黒腐細菌病の生物的防除のためのPseudomonas sp. BM2-6株の効果的な処理方法 Reviewed
横山遼人, 鈴木千尋, 服部雄斗, 富田 駿, 横田健治, キムオッキョン, 岩波 徹, 篠原弘亮
東京農業大学農学集報 68 ( 1 ) 13 - 22 2023.06
Language：Japanese Publishing type：Research paper (scientific journal)
著者ら（2018）はエノキタケ黒腐細菌病（病原：Pseudomonas tolaasii）に対し発病抑制効果のある P. sp. BM2-6 株を見出した。本研究では BM2-6 株の有効な処理方法を検討し，発病抑制機構を考察した。 BM2-6 株細菌懸濁液のエノキタケ（Flammulina velutipes）幼子実体への噴霧処理または菌掻き時処理した ところ，防除価はいずれも高く，約 109 cfu/ml において 99.6，98.4 であった。約 106 cfu/ml において 83.3， 90.3 であった。約 1010 cfu/mL または約 106 cfu/mL の BM2-6 株細菌懸濁液処理および約 105 cfu/mL の P. tolaasii 814 株細菌懸濁液接種におけるエノキタケ菌床または子実体での両菌の各細菌数を調査した。これ らエノキタケに発病は認められず，菌床における BM2-6 株は各処理ともに約 108 cfu/g, P. tolaasii 814 株は それぞれ約 106 cfu/g, 約 107 cfu/g 定着していた。子実体における両菌は各処理ともそれぞれ約 107 cfu/g, 約 108 cfu/g 定着していた。BM2-6 株はトラシン解毒能も持たないことから，液体培地中で両菌を混合培養し， 両細菌数および腐敗の主因であるトラシンⅠの量を測定した。P. tolaasii 814 株の単独培養時，約 1010 cfu/ mL を超えるとトラシンⅠが最大 45.9 µg/mL 検出された。一方，混合培養では，両菌ともに約 1010 cfu/mL まで増殖したがトラシンⅠは非検出であったことから，BM2-6 株の発病抑制機構は P. tolaasii 814 株のトラ シン産生抑制であると判断した。以上，BM2-6 株は菌掻き時処理により本病を効果的に防除でき，P. tolaasii のトラシン産生を抑制することによって発病を抑制した。
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Strategies for Biological Control of Brown Blotch Disease
TOMITA Shun, SHINOHARA Hirosuke, YOKOTA Kenji
KAGAKU TO SEIBUTSU 61 ( 3 ) 116 - 124 2023.03
Language：Japanese Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry
Books and Other Publications 【 display / non-display 】
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太田寛行，森﨑久雄，大塚重人，渡邉健史，鮫島玲子，中川達功，佐伯雄一，礒井俊行，横田健治，豊田剛己，村瀬 潤，浦嶋泰文，藤井一至，原田直樹，西澤智康，浅川 晋，竹腰 恵（ Role： Contributor）
朝倉書店 2018.08 （ ISBN:978-4-254-43571-9 ）
Total pages：208 Responsible for pages：80-87 Language：Japanese Book type：Scholarly book
植物生育促進根圏微生物について解説した。
担当執筆分：8. 有用微生物3―植物生育促進根圏微生物―
A5版 -
里山の自然とくらし 福島県鮫川村
入江 彰昭, 上地 由朗, 内田 均, 竹内 将俊, 舘 博, 安藤 達彦, 穂坂 賢, 高橋 悟, 渡邉 文雄, 川上 昭太郎, 栗田 和弥, 桝田 信彌, 伊藤 健, 入江 満美, 横田 健治（ Role： Joint author , p78-79、2頁）
東京農業大学出版会 2013.03 （ ISBN:978-4-88694 ）
Language：Japanese Book type：Scholarly book
村内の田畑の土壌分析を行い、本地域の土壌の特性について解説した。四六判、P.112
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Sustainable Farming Practices for Environmental Conservation
Edited by Machito Mihara and Akimi Fujimoto（ Role： Joint author , 抽出不可能）
Institute of Environment Rehabilitation and Conservation, Japan 2009.04 （ ISBN:4-916174-08-9 ）
Language：English Book type：Scholarly book
Misc 【 display / non-display 】
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Strategies for Biological Control of Brown Blotch Disease: Microorganisms Protect Edible Mushrooms from Pathogens via a Range of Modes of Action. Invited Reviewed
Shun Tomita, Hirosuke Shinohara, Kenji Yokota
116 - 124 2023.03
Authorship：Last author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Bacillus属細菌を用いた植物病害生物防除における環状リポペプチドの機能 Invited
横田 健治
日本農薬学会誌 47 ( 2 ) 124 - 125 2022.08
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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きのこを褐変化させる毒素tolaasinを解毒する細菌 Invited Reviewed
横田健治・富田駿・篠原弘亮
バイオサイエンスとインダストリー 79 ( 3 ) 214 - 215 2021.05
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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拮抗性Bacillus属細菌を利用した植物病害の生物防除 機能性物質とその防除メカニズムを中心に
横田 健治
土と微生物 66 ( 1 ) 27 - 31 2012.04
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：日本土壌微生物学会
拮抗性Bacillus属細菌を利用した植物病害の生物防除について報告した。
Industrial Property Rights 【 display / non-display 】
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抗菌剤、農薬、微生物による植物病害の防除方法、および新規バチルス - サブティリス
原 富次郎, 高塚 由美子, 杉村 祐哉, 横田 健治
Application no：WO2017150556A1 Date applied：2017.02
Patent/Registration no：WO2017150556A1 Date registered：2017.09 Date issued：2017.09
Country of applicant：Domestic Country of acquisition：Domestic
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分生胞子増殖因子、分生胞子増殖因子を含む培地及び分生胞子増殖因子の製造方法
横田 健治
Applicant：東京農業大学
Application no：特願2008-263233(P2008-263233) Date applied：2008.10
Announcement no：特開2010-88380(P2010-88380A) Date announced：2010.04
Country of applicant：Domestic
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ANTIMICROBIAL AGENT, PESTICIDE, METHOD FOR PREVENTING PLANT DISEASE CAUSED BY MICROORGANISMS, AND NOVEL Bacillus subtilis
Tomijiro Hara, Yumiko Takatsuka, Yuya Sugimura, Kenji Yokota
Application no：15579831
Honours, Awards and Prizes 【 display / non-display 】
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Best Oral Presenter
2022.11 ISSAAS-SEAPPRO Joint International Seminar and Congress 2022
Award type：Award from international society, conference, symposium, etc. Country：Indonesia
Scientific Research Funds Acquisition Results 【 display / non-display 】
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病害土壌診断バイオマーカーの開発に向けたメタゲノム解析
2016.09 - 2019.08
科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
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地域資源活用型小規模持続的農業システムのためのアグロエコロジー研究
2016.04 - 2019.03
科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
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バチルス属菌由来の環状リポペプチドによるシロイヌナズナの病害抵抗性制御機構の解明
2016.04 - 2019.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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黄色ブドウ球菌の血球崩壊毒素の三次元構造解析による膜孔形成機構の解明
2000.04 - 2001.03
科学研究費補助金 特別研究員推奨費
黄色ブドウ球菌が生産する二成分性膜孔形成型毒素タンパク質γヘモリジンの構成分子LukF成分の赤血球細胞認識及び結合領域の解明
Other External Funds 【 display / non-display 】
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Bacillus属が分泌生産する新規抗菌性環状リポペプチドの構造解析と抗菌活性評価
2020.04 - 2021.03
京都大学エネルギー理工学研究所
横田 健治
Authorship：Principal investigator Grant type：Competitive
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Bacillus属が分泌生産する新規抗菌性環状リポペプチドの構造解析と抗菌活性評価
2019.04 - 2020.03
京都大学エネルギー理工学研究所
横田 健治
Authorship：Principal investigator Grant type：Competitive
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インドネシアのマングローブ根圏から分離された各種拮抗性細菌の拮抗作用の解明
2018.11 - 2019.02
民間財団等
横田 健治
Authorship：Principal investigator Grant type：Competitive
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Bacillus属菌の抗菌性リポペプチド生産を誘導する大豆由来の生理活性物質に関する研究
2010.05 - 2011.04
民間財団等 タカノ農芸化学研究助成財団 若手研究者部門
Authorship：Principal investigator Grant type：Competitive
Presentations 【 display / non-display 】
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Microbacterium foliorum NBRC 103072Tによるtolaasin 解毒機構 -Tolaasin 加水分解酵素は菌体の界面 活性剤処理により可溶化する- International conference
富田 駿, 梶川揚申, 五十君靜信, 篠原弘亮, 横田健治
令和2 年度日本植物病理学会大会 2020.03
Event date： 2020.03
Language：English Presentation type：Oral presentation (general)
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Susceptibility to Tolaasins on Tolaasin-Detoxyfying Microbacterium spp. International conference
Shun Tomita, Hirosuke Shinohara, Kenji Yokota
ISSAAS 2019 International Congress & General Meeting 2019 2019.10
Event date： 2019.10
Language：English Presentation type：Oral presentation (general)
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Comparative Study of Surfactin Homologues on Antibacterial Activity International conference
Takafumi Yoshida and Kenji Yokota
ISSAAS 2019 International Congress & General Meeting 2019 2019.10
Event date： 2019.10
Language：English Presentation type：Oral presentation (general)
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Arthrobacter sp. Isolate Enhance Fusarium Soil-Borne Disease International conference
Mana Adachi, Ryota Kataoka, Yusuke Yamamoto, Ryoko Kato, Kenji Yokota
ISSAAS 2019 International Congress & General Meeting 2019 2019.10
Event date： 2019.10
Language：English Presentation type：Oral presentation (general)
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Analysis of the Inhibitory Effect of Bacterial Growth on Cabbage Leaves in Response to Treatment with Lipopeptides International conference
Carla Suzuki Altrão, Yusuke Yanai, Hirosuke Shinohara, Kenji Yokota
ISSAAS 2019 International Congress & General Meeting 2019 2019.10
Event date： 2019.10
Language：English Presentation type：Oral presentation (general)