Updated on 2022/11/05

写真a

YOKOTA Kenji

Professor

Title

Professor

External Link

From Graduate School 【 display / non-display

  • Tohoku University   Graduate School, Division of Agriculture   Doctoral program (second term)   Completed

    1996.04 - 2000.03

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    Country:Japan

  • Tokyo University of Agriculture   Graduate School, Division of Agriculture   Master Course   Completed

    1994.04 - 1996.03

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    Country:Japan

Studying abroad experiences 【 display / non-display

  • 2012.04 - 2013.03   University of California, Berkeley   Visiting scholar

Degree 【 display / non-display

  • 博士(農学) ( 2000.03   東北大学 )

Employment Record in Research 【 display / non-display

  • Tokyo University of Agriculture   Faculty of Applied Bio-Science   Department of Applied Biology and Chemistry   Research Assistant

    2001.04 - 2007.03

  • Tokyo University of Agriculture   Faculty of Applied Bio-Science   Department of Applied Biology and Chemistry   Assistant Professor

    2007.04 - 2012.09

  • Tokyo University of Agriculture   Faculty of Applied Bio-Science   Department of Applied Biology and Chemistry   Associate Professor

    2012.10 - 2018.03

  • Tokyo University of Agriculture   Faculty of Applied Bio-Science   Department of Agricultural Chemistry   Associate Professor

    2018.04 - 2019.03

  • Tokyo University of Agriculture   Faculty of Applied Bio-Science   Department of Agricultural Chemistry   Professor

    2019.04

External Career 【 display / non-display

  • 東北大学大学院   農学研究科農芸化学専攻   日本学術振興会特別研究員

    2000.04 - 2001.03

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    Country:Japan

Research Areas 【 display / non-display

  • Life Science / Applied microbiology

Papers 【 display / non-display

  • <i>Bacillus</i>属細菌を用いた植物病害生物防除における環状リポペプチドの機能

    横田 健治

    日本農薬学会誌   47 ( 2 )   124 - 125   2022.08

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    Language:Japanese   Publisher:日本農薬学会  

    DOI: 10.1584/jpestics.w22-11

  • Insights on suppression of bacterial leaf spot by Bacillus cyclic lipopeptides via induced resistance in Arabidopsis thaliana Reviewed

    Carla Suzuki Altrão, Mana Kaneko, Shigeaki Shiina, Akinobu Kajikawa, Hirosuke Shinohara, Kenji Yokota

    Journal of General Plant Pathology   2022.03

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    We evaluated disease suppression by surfactin and iturin A against Pseudomonas syringae pv. maculicola MAFF 302783 in Arabidopsis thaliana and its mutants to gain insight into resistance pathways induced. Root treatments revealed that salicylic acid signaling via the isochorismate pathway was the major signaling pathway elicited by both cyclic lipopeptides. Disease suppression was negated after treatment with higher levels of surfactin or iturin A, and this negation of disease suppression was not associated with an antagonistic effect by induction of jasmonate signaling pathway in host plants.

    DOI: https://doi.org/10.1007/s10327-022-01062-9

  • Comparative study of disease suppression on various host plants by Bacillus cyclic lipopeptides Reviewed

    Carla Suzuki Altrão, Takuya Kawashima, Minami Ohbu, Shuhei Matsuura, Misaki Higuchi, Yusuke Yanai, Yuki Hase, Hirosuke Shinohara, Kenji Yokota

    Agricultural Sciences   13   2022.01

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    In biological controls using Bacillus spp., cyclic lipopeptides play a role as elicitors to induce disease resistance on various host plants. However, it is still unclear the specificity between cyclic lipopeptides and host plants to induce disease resistance. In this study, we aimed to clarify the specificity to induce disease resistance among cyclic lipopeptides on various host plants. Our data clearly showed both cyclic lipopeptides conferred disease suppression on most of host plants, but at different range of cyclic lipopeptide concen-tration. Our findings contribute to understanding the complex on the specificity of cy-clic lipopeptide derived induced disease resistance.

    DOI: https://doi.org/10.4236/as.2022.131001

  • Draft Genome Sequence of the Polychlorinated Biphenyl Degrader Comamonas testosteroni Strain YAZ2 Isolated from a Natural Landscape in the Tohoku Region of Japan Reviewed International journal

    Tomijiro Hara, Yumiko Takatsuka, Yuh Shiwa, Kenji Yokota

    Microbiology Resource Announcements   11 ( 1 )   e00806-21   2022.01

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    Language:English   Publishing type:Research paper (scientific journal)  

    We report a draft genome sequence of Comamonas testosteroni strain YAZ2, a polychlorinated biphenyl (PCB) degrader that was isolated from a PCB-unpolluted
    environment. The assembled genome contains a single 5.4-Mb chromosome and an 87-kb plasmid. The bph gene cluster, which is involved in PCB degradation, was
    found on the chromosome.

    DOI: https://doi.org/10.1128/mra.00806-21

  • Insights into detoxification of tolaasins, the toxins behind mushroom bacterial blotch, by Microbacterium foliorum NBRC 103072T Reviewed International journal

    Shun Tomita, Akinobu Kajikawa, Shizunobu Igimi, Hirosuke Shinohara, Kenji Yokota

    PhytoFrontiers   2021.03

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Tolaasins are lipodepsipeptides secreted by Pseudomonas tolaasii, the causal agent of brown blotch disease of mushrooms, and are the toxins that cause the brown spots. We previously reported that Microbacterium foliorum NBRC 103072T is an effective tolaasin-detoxifying bacterium. In this study, we aimed to characterize the tolaasin-detoxification process of M. foliorum NBRC 103072T. The tolaasin-detoxification by M. foliorum NBRC 103072T was carried out by hydrolyzation of tolaasins at two specific sites in the peptide moiety of tolaasins by its cells, and the resulting fragments were released from bacterial cells. The tolaasin-hydrolyzing activity can be extracted by neutral detergent solution from M. foliorum NBRC 103072T cells. Moreover, tolaasin-adsorption to the bacterial cells occurred prior to hydrolyzation of tolaasins, which might contribute to the effective tolaasin-detoxification by M. foliorum NBRC 103072T. It is notable that the tolaasin-degradation process by M. foliorum NBRC 103072T is carried out by hydrolyzation at specific sites in the peptide moiety of lipopeptide by bacterial cells as a novel biological degradation process of cyclic lipopeptides.

    DOI: https://doi.org/10.1094/PHYTOFR-10-20-0024-R

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Books and Other Publications 【 display / non-display

  • 土壌微生物学

    太田寛行,森﨑久雄,大塚重人,渡邉健史,鮫島玲子,中川達功,佐伯雄一,礒井俊行,横田健治,豊田剛己,村瀬 潤,浦嶋泰文,藤井一至,原田直樹,西澤智康,浅川 晋,竹腰 恵( Role: Contributor)

    朝倉書店  2018.08  ( ISBN:978-4-254-43571-9

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    Total pages:208   Responsible for pages:80-87   Language:Japanese   Book type:Scholarly book

    植物生育促進根圏微生物について解説した。
    担当執筆分:8. 有用微生物3―植物生育促進根圏微生物―
    A5版

    Other Link: https://www.amazon.co.jp/%E5%9C%9F%E5%A3%8C%E5%BE%AE%E7%94%9F%E7%89%A9%E5%AD%A6-%E5%AE%9F%E8%B7%B5%E5%9C%9F%E5%A3%8C%E5%AD%A6%E3%82%B7%E3%83%AA%E3%83%BC%E3%82%BA-%E8%B1%8A%E7%94%B0-%E5%89%9B%E5%B7%B1/dp/4254435711/ref=sr_1_1?ie=UTF8&qid=1535429658&sr=8-1&keywords=%E5%9C%9F%E5%A3%8C%E5%BE%AE%E7%94%9F%E7%89%A9%E5%AD%A6

  • 里山の自然とくらし 福島県鮫川村

    入江 彰昭, 上地 由朗, 内田 均, 竹内 将俊, 舘 博, 安藤 達彦, 穂坂 賢, 高橋 悟, 渡邉 文雄, 川上 昭太郎, 栗田 和弥, 桝田 信彌, 伊藤 健, 入江 満美, 横田 健治( Role: Joint author ,  p78-79、2頁)

    東京農業大学出版会  2013.03  ( ISBN:978-4-88694

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    Language:Japanese   Book type:Scholarly book

    村内の田畑の土壌分析を行い、本地域の土壌の特性について解説した。四六判、P.112

  • Sustainable Farming Practices for Environmental Conservation

    Edited by Machito Mihara and Akimi Fujimoto( Role: Joint author ,  抽出不可能)

    Institute of Environment Rehabilitation and Conservation, Japan  2009.04  ( ISBN:4-916174-08-9

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    Language:English   Book type:Scholarly book

Misc 【 display / non-display

  • Bacillus属細菌を用いた植物病害生物防除における環状リポペプチドの機能 Invited

    横田 健治

    日本農薬学会誌   47 ( 2 )   124 - 125   2022.08

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: https://doi.org/10.1584/jpestics.W22-11

  • きのこを褐変化させる毒素tolaasinを解毒する細菌 Invited Reviewed

    横田健治・富田駿・篠原弘亮

    バイオサイエンスとインダストリー   79 ( 3 )   214 - 215   2021.05

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 拮抗性Bacillus属細菌を利用した植物病害の生物防除 機能性物質とその防除メカニズムを中心に

    横田 健治

    土と微生物   66 ( 1 )   27 - 31   2012.04

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (international conference proceedings)   Publisher:日本土壌微生物学会  

    拮抗性Bacillus属細菌を利用した植物病害の生物防除について報告した。

    DOI: https://doi.org/10.18946/jssm.66.1_27

Industrial Property Rights 【 display / non-display

  • 抗菌剤、農薬、微生物による植物病害の防除方法、および新規バチルス - サブティリス

    原 富次郎, 高塚 由美子, 杉村 祐哉, 横田 健治

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    Application no:WO2017150556A1  Date applied:2017.02

    Patent/Registration no:WO2017150556A1  Date registered:2017.09  Date issued:2017.09

    Country of applicant:Domestic   Country of acquisition:Domestic

  • 分生胞子増殖因子、分生胞子増殖因子を含む培地及び分生胞子増殖因子の製造方法

    横田 健治

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    Applicant:東京農業大学

    Application no:特願2008-263233(P2008-263233)  Date applied:2008.10

    Announcement no:特開2010-88380(P2010-88380A)  Date announced:2010.04

    Country of applicant:Domestic  

Honours, Awards and Prizes 【 display / non-display

  • Best Oral Presenter

    2022.11   ISSAAS-SEAPPRO Joint International Seminar and Congress 2022  

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    Award type:Award from international society, conference, symposium, etc.  Country:Indonesia

Scientific Research Funds Acquisition Results 【 display / non-display

  • 病害土壌診断バイオマーカーの開発に向けたメタゲノム解析

    2016.09 - 2019.08

    科学研究費補助金  基盤研究(C)

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    Authorship:Coinvestigator(s) 

  • 地域資源活用型小規模持続的農業システムのためのアグロエコロジー研究

    2016.04 - 2019.03

    科学研究費補助金  基盤研究(C)

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    Authorship:Coinvestigator(s) 

  • バチルス属菌由来の環状リポペプチドによるシロイヌナズナの病害抵抗性制御機構の解明

    2016.04 - 2019.03

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

  • 黄色ブドウ球菌の血球崩壊毒素の三次元構造解析による膜孔形成機構の解明

    2000.04 - 2001.03

    科学研究費補助金  特別研究員推奨費

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    黄色ブドウ球菌が生産する二成分性膜孔形成型毒素タンパク質γヘモリジンの構成分子LukF成分の赤血球細胞認識及び結合領域の解明

Other External Funds 【 display / non-display

  • Bacillus属が分泌生産する新規抗菌性環状リポペプチドの構造解析と抗菌活性評価

    2020.04 - 2021.03

    京都大学エネルギー理工学研究所 

    横田 健治

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    Authorship:Principal investigator  Grant type:Competitive

  • Bacillus属が分泌生産する新規抗菌性環状リポペプチドの構造解析と抗菌活性評価

    2019.04 - 2020.03

    京都大学エネルギー理工学研究所 

    横田 健治

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    Authorship:Principal investigator  Grant type:Competitive

  • インドネシアのマングローブ根圏から分離された各種拮抗性細菌の拮抗作用の解明

    2018.11 - 2019.02

    民間財団等 

    横田 健治

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    Authorship:Principal investigator  Grant type:Competitive

  • Bacillus属菌の抗菌性リポペプチド生産を誘導する大豆由来の生理活性物質に関する研究

    2010.05 - 2011.04

    民間財団等  タカノ農芸化学研究助成財団 若手研究者部門 

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    Authorship:Principal investigator  Grant type:Competitive

Presentations 【 display / non-display

  • Microbacterium foliorum NBRC 103072Tによるtolaasin 解毒機構 -Tolaasin 加水分解酵素は菌体の界面 活性剤処理により可溶化する- International conference

    富田 駿, 梶川揚申, 五十君靜信, 篠原弘亮, 横田健治

    令和2 年度日本植物病理学会大会  2020.03 

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    Event date: 2020.03

    Language:English   Presentation type:Oral presentation (general)  

  • Comparative Study of Surfactin Homologues on Antibacterial Activity International conference

    Takafumi Yoshida and Kenji Yokota

    ISSAAS 2019 International Congress & General Meeting 2019  2019.10 

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    Event date: 2019.10

    Language:English   Presentation type:Oral presentation (general)  

  • Arthrobacter sp. Isolate Enhance Fusarium Soil-Borne Disease International conference

    Mana Adachi, Ryota Kataoka, Yusuke Yamamoto, Ryoko Kato, Kenji Yokota

    ISSAAS 2019 International Congress & General Meeting 2019  2019.10 

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    Event date: 2019.10

    Language:English   Presentation type:Oral presentation (general)  

  • Analysis of the Inhibitory Effect of Bacterial Growth on Cabbage Leaves in Response to Treatment with Lipopeptides International conference

    Carla Suzuki Altrão, Yusuke Yanai, Hirosuke Shinohara, Kenji Yokota

    ISSAAS 2019 International Congress & General Meeting 2019  2019.10 

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    Event date: 2019.10

    Language:English   Presentation type:Oral presentation (general)  

  • Susceptibility to Tolaasins on Tolaasin-Detoxyfying Microbacterium spp. International conference

    Shun Tomita, Hirosuke Shinohara, Kenji Yokota

    ISSAAS 2019 International Congress & General Meeting 2019  2019.10 

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    Event date: 2019.10

    Language:English   Presentation type:Oral presentation (general)  

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