Papers - SHINOHARA Takashi
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Influence of the Hypobaric Method in Physicochemical Fruit Quality Traits of Yellow and Purple Passion Fruit Stored in Cold Temperature Reviewed International coauthorship International journal
Viera William, Shinohara Takashi, Sanada Atsushi, Terada Naoki, Koshio Kaihei
The Horticulture Journal advpub ( 0 ) 2023
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：The Japanese Society for Horticultural Science
<p>Passion fruit (<i>Passiflora edulis</i>) is a tropical fruit that can be consumed fresh or processed. It is a typical respiratory climacteric fruit which is highly perishable because of the loss of water that causes dehydration and thus shrinkage, affecting the fruit appearance; in addition, some quality traits such as fruit weight, firmness and vitamin C content can also be influenced. Therefore, this fruit has a short shelf life. Storage in low temperatures has been assessed to extent the passion fruit shelf life remaining its fruit quality traits; however, information about the application of the hypobaric method in this fruit is null. The objective of this research was to evaluate the physical and chemical characteristics of the yellow and purple passion fruit under hypobaric storage conditions in low temperature. Results showed the hypobaric method had a positive effect in decreasing fruit weight loss, declining the loss of firmness, and reducing the degradation of vitamin C during the storage period. It decreased the evolution of ethylene which is positive to delay fruit senescence, and the production of CO<sub>2</sub>. Finally, it was the only method which avoids the shrinkage completely in the purple passion fruits (‘Gulupa’, ‘Summer Queen’, and ‘Ruby Star’) and showed minor shrinkage in ‘POR1’ (yellow passion fruit). This method is considered as a promising technique to improve fruit storage.</p>
DOI： 10.2503/hortj.qh-081
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Development of Simple Sequence Repeat (SSR) and Morphological Markers to Identify Jaboticaba Cultivars Reviewed International journal
SHINOHARA Takashi, HONDA Shunsuke, YOSHIDA Saki, OHO Kiyotaka, IGARASHI Daizo, WAKUI Kenji, OHTAKE Rumi, TANAKA Keisuke
Tropical Agriculture and Development 65 ( 2 ) 68 - 74 2021
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society for Tropical Agriculture
<p>Jaboticaba, belonging to Myrtaceae family, has been a minor tropical fruit tree in Japan and three cultivars, ‘Sabará’, ‘Açu’ and ‘Miúda’, have been distributed. The cultivars are clearly distinguished by their bearing habits and traits of fruits. As Jaboticaba has polyembryony, it is possible to propagate the cultivars in large amounts if techniques selecting seedlings that generated from apomictic embryos exist. This research aims to develop simple sequence repeat (SSR) and morphological markers. Over 50,000 SSRs in the genome extracted from ‘Sabará’ were cataloged using the microsatellite capture sequencing technique, and one SSR primer set was developed for the identification of the three cultivars. Each unique fragment for ‘Açu’ and ‘Miúda’ in the PCR products may be non-specific fragment such as due to ascribed by different genotypes or homologues in flanking SSR region, but then the primer set indicated high robustness because of profiling consistently within and among cultivars. The three jaboticaba cultivars can be also classified by morphological traits of the leaf. ‘Sabará’ has significantly larger leaflets, which have uneven surface and come out from the rachis downwards, than other two cultivars. The size of leaflet is not different significantly between ‘Açu’ and ‘Miúda’, but their leaflet color at young stage differs clearly. The former is green, and the latter cultivar is red. These morphological traits would be influenced by ambient environments but these are practical to classify the three jaboticaba cultivars at the juvenile phase.</p>
DOI： 10.11248/jsta.65.68
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Estimating irrigation water amounts for tomato cultivation using observed meteorological data in Djibouti Reviewed
WATANABE Fumio, SUZUKI Shinji, MOHAMED Tabarek Ismael, JITSUNO Masataka, OHSHIMA Hiroyuki, SANADA Atsushi, SHINOHARA Takashi
Journal of Arid Land Studies 32 ( 3 ) 81 - 81 2022.12
Language：English Publisher：The Japanese Association for Arid Land Studies
<p>Irrigation is essential for crop cultivation in dry and semi-arid areas with limited rainfall and water resources. Efficient and effective use of water resources is required but saving irrigation water is important. The study area is in Ali Faren farm located in west of Djibouti which lies in the Horn of Africa. Djibouti is a very dry region with annual rainfall of about 150 mm and an average annual temperature of 28.6°C. Due to such meteorological conditions, water resources depend mostly on groundwater, and this makes water extremely scare. Therefore, water saving in the agricultural field is indispensable.</p><p>This study aims at effective and efficient use of irrigation water in Djibouti. The appropriate irrigation water for tomato cultivation were estimated using CROPWAT provided by FAO and observed meteorological data at Ali Faren farm in Djibouti. The observed meteorological data include air temperature, relative humidity, wind speed and sunshine hour. The conditions required for calculating the amount of irrigation water were medium loam soil texture based on the laboratory results of soil particle size test, and the tomato cultivation period from October to March according to the local farmers.</p><p>The result showed that the average monthly reference evapotranspiration was 5.7 mm/day annually. Reference Evapotranspiration was maximum in July with 7.9 mm/day and minimum in December with 3.9 mm/day. It was observed that the effective rainfall from October to March was 55.2 mm which corresponds to the local tomato cultivation period. Additionally, the required water amount for tomato crops varied between 2.9 to 4.2 mm/day.</p><p>Finally, the appropriate irrigation area of this farm will be determined based on the measured amount of groundwater discharge at an existing well and the estimated irrigation water amount.</p>
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Pulp Mineral Content of Passion Fruit Germplasm Grown in Equador and Its Relationship with Fruit Qualiy Traits Reviewed
697 1 - 19 2022.03
Language：English Publishing type：Research paper (scientific journal)
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Phytochemical Composition and Antioxidant Activity of Passiflora spp. Germplasm Grown in Ecuador Reviewed
328 1 - 19 2022.01
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Elucidation of Physiochemical Changes in Fruit Development of 'Sabara' Jaboticaba (Plinia cauliflora (Mart.) Kausel) Reviewed
印刷中 2022.01
Language：English Publishing type：Research paper (scientific journal)
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Effects of Temperature on Pollen Germination of 'Sabara' Jaboticaba (Plinia cauliflora (Mart.) Kausel) in vitro Reviewed
印刷中 2022.01
Language：English Publishing type：Research paper (scientific journal)
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Early Counts of Radicle Emergence, Counted Matually and by image analysis, Can Reveal Different in the Production of Normal Seedlings and the Vigour of Seed Lots of Cauliflower Reviewed
49 ( 3 ) 219 - 235 2021.12
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Development of Simple Sequence Repeat (SSR) and Morphological Markers to Identify Jaboticaba Cultivars Reviewed
65 ( 2 ) 68 - 74 2020.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Growth, Quality and Capsaicin Concentration of Hot Pepper (Capsicum annuum) under Drought Conditions Reviewed
26 ( 1 ) 100 - 110 2020.06
Language：English Publishing type：Research paper (scientific journal)
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The effects of a coating treatment of weight loss, juice acidity, shrinkage and ethylene evolution of premature passion fruit: a preliminary experiment Reviewed International journal
Takashi Shinohara, Kiyotaka Oho, Daizou Igarashi, Kaihei Koshio
Tropical Agriculture and Development 63 ( 4 ) 219 - 222 2019.12
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Ethylene evolution and premature drop in passion fruit Reviewed
Takashi Shinohara, Mizuki Usui, Ayaka Sabanai, Atsushi Sanada, Daizou Igarashi, Kaihei Koshio
Tropical Agriculture and Development 63 ( 3 ) 105 - 112 2019.09
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Effective DNA fragmentation technique for simple sequence repeat detection with a microsatelite-enriched library and high-throughput sequencing Reviewed
Keisuke Tanaka, Rumi Ohtake, Saki Yoshida and Takashi Shinohara
BioTechniques 62 180 - 182 2017.04
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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花穂整形方法を異にするブドウ’藤稔’の摘心と環状剥皮が果粒肥大、品質に及ぼす影響 Reviewed
石川一憲、馬場正、藤沢弘幸、篠原卓、関達哉、山口正己
農作業研究 51 ( 3 ) 101 - 107 2016.09
Language：Japanese Publishing type：Research paper (scientific journal)
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Effect of Accumulated Minimum Temperature on Sugar and Organic Acid Content in Passionfruit 〔最低気温の積算温度がパッションフルーツ果汁の糖および有機酸含量に及ぼす影響〕 Reviewed
Shinohara T. Usui M. Higa Y. Igarashi D. and Inoue T.
Journal of International Society for Southeast Asian Agricultural Sciences 19 ( 2 ) 1 - 7 2013.12
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
パッションフルーツ（品種サマークイーン）の果実発育期間の長さは、同期間の平均最低気温と高い相関があることを明らかにした。そのため、果実発育期間中の最低気温の積算温度を指標として果汁の糖および酸の変化を初めて示した。その結果、果実発育期間中の最低気温の積算温度が1350℃日に達すると、果汁の全糖含量が15g/100mLで酸度が3g/100mL未満となり生食に向く果実となることが明らかとなった。
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鮫川村のダイズ生産安定に関する研究-鮫川村で生産されたダイズの種子活力調査- Invited
篠原卓、井上綾、桑田悠斐、五十嵐大造、井上知昭
食農と環境 12 53 - 57 2013.09
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Relationship between Accumulated Temperature during Fruit Development and the Total Organic Acid Content in 'Summer Queen' Passion Fruit (Passiflora edulis x P. edulis f. flavicarpa) [パッションフルーツ品種サマークイーンにおける果実発育期間中の積算温度と果汁の有機酸含量の関係] Reviewed
Shinohara T. Higa Y. Usui M. Igarashi D. and Inoue T.
Tropical Agriculture and Development 57 ( 2 ) 74 - 76 2013.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
パッションフルーツ（品種サマークイーン）夏実と秋実の収穫時の果汁酸度と果実発育期間中の積算温度の関係を検討し、両者には高い負の相関があることを明らかにした。夏実と秋実では、収穫時の果汁酸度が3g/100mLとなるために、10℃以上の積算温度がそれぞれ1204℃日と939℃日必要であった。パッションフルーツは自然落果した果実を収穫するが、果実発育期間中の積算温度を測定することで非破壊でも果汁酸度を推定することができると考えられた。また、夏実生産において果汁酸度が高いまま落果する早期落果を多く観察した。
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Genic Male Sterility in Brassica rapa spp. rapa cv. 77B 〔カブ品種77Bにおける核遺伝子型雄性不稔〕
Wakui K. Shinohara T. Fujihara E. Komatsu K. Igarashi D. Fujigaki J.
東京農業大学集報 57 ( 4 ) 287 - 292 2013.03
Language：English Publishing type：Research paper (bulletin of university, research institution)
カブ品種77Bでみいだされた雄性不稔について、花の形態および遺伝様式を調査した。雄性不稔個体は可稔個体と比較して雄蕊が短く、花弁が小さかった。雄性不稔個体を７７Bの可稔個体および異なる２品種と交配し、その後代の分離比を調査した結果、本雄性不稔性は劣性の一遺伝子座によって支配されていることが示唆された。雄性不稔への細胞質による影響はみられなかった。また、雄性不稔個体における四分子期あるいはより早い時期における四分子形成の崩壊が示唆された。さらに、蕾のアミノ酸解析から雄性不稔個体と比べ可稔個体でプロリン含量の著しい増加が示された。
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Fluoride in Life Water and Health Survey of Farmers in Arid and Semi-arid Basin in Ethiopia
Journal of Japanese Health and Medicine Association 21 ( 2 ) 95 - 100 2012.07
Language：Japanese Publishing type：Research paper (scientific journal)
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Application Example of a Small Solar Pumping System in the Djiboutian Wadi Agriculture 〔ジプチ共和国のワジ農業における小型ソーラーポンピングシステムの実践例〕 Reviewed
Tajima K. Suzuki S. Shinohara T. Sanada A. Watanabe F.
Journal of Arid Land Studies 22 ( 1 ) 337 - 340 2012.06
Language：English Publishing type：Research paper (scientific journal)
ジプチ共和国でオアシス農業を実践する篤農家圃場にソーラーポンピングシステム（680W）を設置し、性能確認調査を行った。最大水量は95L/分であった。一日に使用される水量は90ｍ3/日であり、そのうち39ｍ3/日はソーラーポンピングシステムで賄っていた。
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Effects of Seed Vigour on Field Emergence and Early Seedling Growth of Maize under Arid Tropical Conditions 〔熱帯乾燥地における種子活力の違いがトウモロコシの出芽および初期生育に及ぼす影響〕 Reviewed
Shinohara T. Nakasone K. Tajima K. Watanabe F. Shiokura T. Takahashi H.
Tropical Agriculture and Development 53 ( 4 ) 112 - 117 2009.12
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
ジプチ共和国でオアシス農業を実践する篤農家圃場において、種子活力の違い、ストーンマルチ（SM）処理および浸種（PS）処理を組み合わせて行ったトウモロコシの出芽試験の結果である。種子活力の違いは、出芽速度、最終的な出芽率および初期生育に有意に影響した。SM処理とPS処理は、それぞれ単独でも出芽速度を高めたが、この傾向は種子活力の高いロットで低いロットよりも大きかった。
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Effect of Ca removal from Nutrient Solution and Diurnal Change in Vapor Pressure Deficit on the Fruit Cracking and Ethylene Production of Tomatoes in Hydroponic Culture Reviewed
6 67 - 71 2009.06
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
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Pea (Pisum sativum L.) seed vigour is associated with temperature during reproductive growth ［エンドウマメの種子活力は生殖生長期間中の温度条件と関係している］ Reviewed
Shinohara T. Hampton J G. Hill M J. Juntakool S. Sukprakarn S. Sagwansupyakorn C.
Japanese Society for Agricultural Technology Management 14 ( 3 ) 148 - 155 2008.03
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
ニュージーランドの5つの主要種子生産地域において過去4シーズンに生産されたエンドウマメ種子を収集し、Hollow Heart（HH）発生率と電気伝導度（EC）を測定した。HH発生率とECには、年次間差及び地域間差がみられ、それぞれ2月（晩夏）の気温条件と有意な相関関係が存在した。得られた単回帰直線を用い、過去12シーズンの気象データから、各採種地域で生産される種子活力を予測し、高品質種子を生産する地域を提案した。
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Effects of Soil Water Deficiency on Seed Germinability and Vigour during Seed Maturation in Peanut ［結実期間中の土壌水分欠乏がラッカセイの発芽と活力に及ぼす影響］ Reviewed
Shinohara T. Isoda A.
Journal of International Society for Southeast Asian Agricultural Sciences 13(1) 13 ( 1 ) 26 - 32 2007.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
地下結実性を示すラッカセイの結実期間に、土壌水分条件を乾燥した条件で採種した種子は、湿潤に保ち採種した種子に比べて発芽率は低下しなかったが、活力が有意に低下した。また、結実期間中の土壌乾燥は、幼芽が変色した種子を多く発生させ、こうした種子は、発芽後に葉茎部が発達しない異常苗となった。これらのことから、ラッカセイの採種栽培において、結実期間中の土壌は湿潤に保つことが有効だと提案した。
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The location of deterioration within the garden pea (Pisum sativum) cotyledons is associated with the timing of exposure to high temperature. ［エンドウマメ子葉に発生する劣化細胞の位置は、高温にさらされる種子成熟時期と関係する］
Hampton J.G. and Hill M.J.、篠原 卓
New Zealand Journal of Crop and Horticultural Science 34(4) 2006.12
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
高温に被爆する種子成熟時期が異なると、劣化細胞の発生位置は変化するか検討したものである。種子成熟期を５ステージに分け、高温処理（25/30℃：4日間）を行い、採種した種子のHollow heart（HH）発生率、電気伝導度（EC） などを測定した。劣化細胞の位置はテトラゾリウム溶液染色法で行った。種子成熟初期の高温は、子葉向軸側に劣化細胞を発生させ、これがHHとして発現した。種子の生理成熟以降の高温は、子葉背軸側に劣化細胞を発生させ、ECを増加させた。
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The Effects of High Temperature during Seed Development and Maturation on Hollow Heart in Garden Pea (Pisum sativum L.) ［エンドウマメ種子の成熟および登熟期間中の高温がhollow heart発生に及ぼす影響］
Takashi SHINOHARA
Kasetsart University 2006.10
Language：English Publishing type：Thesis (other)
エンドウマメの種子活力を低下させる生理障害であるHollow Heart（HH）を発生させる高温の程度、高温に対して感受性の高い種子成熟期の特定、および発生のメカニズムの解明を行った。その結果、HHは、種子の含水率が70-80％の時期の25℃以上の高温で発生すること、その反応には品種間差があることを明らかにした。子葉向軸側の細胞が、高温によりデンプン蓄積不足を起こすことで発生すると考えられる細胞劣化がHHを誘起すると考えられた。（博士論文）
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Effects of the Field Environment before and after Seed Physiological Maturity on Hollow Heart Occurrence in Garden Pea (Pisum sativum) ［エンドウマメ種子の生理成熟以前および以降の圃場環境要因がhollow heart発生率に及ぼす影響］ Reviewed
Shinohara T. Hampton J G. Hill M J.
New Zealand Journal of Crop and Horticultural Science 34 ( 3 ) 247 - 255 2006.03
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
Hollow heart（エンドウマメ種子の生理障害で圃場出芽を低下させる一要因である；以下HH）を発生させる高温程度と感受性の高い種子成熟時期を特定するために行った圃場試験（３播種期ｘ３品種）である。HH発生率は、種子含水率（SMC）が70-80％である時期の25oC以上の積算温度と正の相関があった。この高温への反応は、品種間で異なった。また、１品種のみSMCが15-25％である時期の高温に反応してHH発生率を増加させた。
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「ラッカセイにおける採種栽培の違いが種子の発芽とその後の生育に及ぼす影響」
篠原 卓
千葉大学 2002.03
Language：Japanese Publishing type：Thesis (other)
ラッカセイを供試し、結実期の土壌乾燥条件は、採取した種子の発芽と活力にどの様な影響を及ぼすのかを、２つの圃場試験により検討した。活力検定は、低温発芽試験、陽イオンおよび糖類の溶出量測定、圃場出芽試験で行った。結実期の土壌の乾燥は、採種した種子の発芽率、特に種子活力を有意に低下させること、幼芽が変色した種子を有意に多く発生させた。このことから、ラッカセイ栽培の盛んな関東地方は、結実期である夏期に、土壌を湿潤に保つことが有効であると提唱した。
（修士論文）