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Professor |
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Laboratory Address |
Sakuragaoka 1-1-1, Setagaya, Tokyo, Japan |
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Laboratory Phone number |
+81-(0)3-5477-2463 |
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Laboratory Fax number |
+81-(0)3-5477-2463 |
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External Link |
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SHINOHARA Takashi Professor |
From School 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Agriculture Graduated
- 2000.03
Country：Japan
From Graduate School 【 display / non-display 】
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Kasetsart大学 熱帯農業研究科 Doctor Course Completed
2003.11 - 2006.10
Country：Thailand
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Chiba University Master Course Completed
2000.04 - 2002.03
Country：Japan
Degree 【 display / non-display 】
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Ph.D. (Tropical Agriculture) ( 2006.11 Kasetsart University )
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Faculty of International Agriculture and Food Studies Department of International Food and Agricultural Science Professor
2023.04
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Tokyo University of Agriculture Faculty of International Agriculture and Food Studies Department of International Agricultural Development
2007.04 - 2008.03
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Tokyo University of Agriculture Junior College Department of Bioproduction Technology Assistant Professor
2008.04 - 2010.03
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Tokyo University of Agriculture Junior College Department of Bioproduction Technology Assistant Professor
2010.04 - 2014.03
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Tokyo University of Agriculture Junior College Department of Bioproduction Technology Associate Professor
2014.04 - 2018.03
Professional Memberships 【 display / non-display 】
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International Seed Testing Association (ISTA)
2008.09
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The Japanese Society for Tropical Agriculture
2008.08
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The Japanese Society for Horticultural Science
2011.01
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The Japanese Association for Arid Land Studies
2009.07
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International Society for Southeast Asian Agricultural Sciences
2007.06
Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Horticultural science / Seed Science Fruit Science
Research Interests 【 display / non-display 】
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horticultural Science
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Fruit Science
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Seed Science
Papers 【 display / non-display 】
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Influence of the Hypobaric Method in Physicochemical Fruit Quality Traits of Yellow and Purple Passion Fruit Stored in Cold Temperature Reviewed International coauthorship International journal
Viera William, Shinohara Takashi, Sanada Atsushi, Terada Naoki, Koshio Kaihei
The Horticulture Journal advpub ( 0 ) 2023
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：The Japanese Society for Horticultural Science
<p>Passion fruit (<i>Passiflora edulis</i>) is a tropical fruit that can be consumed fresh or processed. It is a typical respiratory climacteric fruit which is highly perishable because of the loss of water that causes dehydration and thus shrinkage, affecting the fruit appearance; in addition, some quality traits such as fruit weight, firmness and vitamin C content can also be influenced. Therefore, this fruit has a short shelf life. Storage in low temperatures has been assessed to extent the passion fruit shelf life remaining its fruit quality traits; however, information about the application of the hypobaric method in this fruit is null. The objective of this research was to evaluate the physical and chemical characteristics of the yellow and purple passion fruit under hypobaric storage conditions in low temperature. Results showed the hypobaric method had a positive effect in decreasing fruit weight loss, declining the loss of firmness, and reducing the degradation of vitamin C during the storage period. It decreased the evolution of ethylene which is positive to delay fruit senescence, and the production of CO<sub>2</sub>. Finally, it was the only method which avoids the shrinkage completely in the purple passion fruits (‘Gulupa’, ‘Summer Queen’, and ‘Ruby Star’) and showed minor shrinkage in ‘POR1’ (yellow passion fruit). This method is considered as a promising technique to improve fruit storage.</p>
DOI： 10.2503/hortj.qh-081
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Development of Simple Sequence Repeat (SSR) and Morphological Markers to Identify Jaboticaba Cultivars Reviewed International journal
SHINOHARA Takashi, HONDA Shunsuke, YOSHIDA Saki, OHO Kiyotaka, IGARASHI Daizo, WAKUI Kenji, OHTAKE Rumi, TANAKA Keisuke
Tropical Agriculture and Development 65 ( 2 ) 68 - 74 2021
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society for Tropical Agriculture
<p>Jaboticaba, belonging to Myrtaceae family, has been a minor tropical fruit tree in Japan and three cultivars, ‘Sabará’, ‘Açu’ and ‘Miúda’, have been distributed. The cultivars are clearly distinguished by their bearing habits and traits of fruits. As Jaboticaba has polyembryony, it is possible to propagate the cultivars in large amounts if techniques selecting seedlings that generated from apomictic embryos exist. This research aims to develop simple sequence repeat (SSR) and morphological markers. Over 50,000 SSRs in the genome extracted from ‘Sabará’ were cataloged using the microsatellite capture sequencing technique, and one SSR primer set was developed for the identification of the three cultivars. Each unique fragment for ‘Açu’ and ‘Miúda’ in the PCR products may be non-specific fragment such as due to ascribed by different genotypes or homologues in flanking SSR region, but then the primer set indicated high robustness because of profiling consistently within and among cultivars. The three jaboticaba cultivars can be also classified by morphological traits of the leaf. ‘Sabará’ has significantly larger leaflets, which have uneven surface and come out from the rachis downwards, than other two cultivars. The size of leaflet is not different significantly between ‘Açu’ and ‘Miúda’, but their leaflet color at young stage differs clearly. The former is green, and the latter cultivar is red. These morphological traits would be influenced by ambient environments but these are practical to classify the three jaboticaba cultivars at the juvenile phase.</p>
DOI： 10.11248/jsta.65.68
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Estimating irrigation water amounts for tomato cultivation using observed meteorological data in Djibouti Reviewed
WATANABE Fumio, SUZUKI Shinji, MOHAMED Tabarek Ismael, JITSUNO Masataka, OHSHIMA Hiroyuki, SANADA Atsushi, SHINOHARA Takashi
Journal of Arid Land Studies 32 ( 3 ) 81 - 81 2022.12
Language：English Publisher：The Japanese Association for Arid Land Studies
<p>Irrigation is essential for crop cultivation in dry and semi-arid areas with limited rainfall and water resources. Efficient and effective use of water resources is required but saving irrigation water is important. The study area is in Ali Faren farm located in west of Djibouti which lies in the Horn of Africa. Djibouti is a very dry region with annual rainfall of about 150 mm and an average annual temperature of 28.6°C. Due to such meteorological conditions, water resources depend mostly on groundwater, and this makes water extremely scare. Therefore, water saving in the agricultural field is indispensable.</p><p>This study aims at effective and efficient use of irrigation water in Djibouti. The appropriate irrigation water for tomato cultivation were estimated using CROPWAT provided by FAO and observed meteorological data at Ali Faren farm in Djibouti. The observed meteorological data include air temperature, relative humidity, wind speed and sunshine hour. The conditions required for calculating the amount of irrigation water were medium loam soil texture based on the laboratory results of soil particle size test, and the tomato cultivation period from October to March according to the local farmers.</p><p>The result showed that the average monthly reference evapotranspiration was 5.7 mm/day annually. Reference Evapotranspiration was maximum in July with 7.9 mm/day and minimum in December with 3.9 mm/day. It was observed that the effective rainfall from October to March was 55.2 mm which corresponds to the local tomato cultivation period. Additionally, the required water amount for tomato crops varied between 2.9 to 4.2 mm/day.</p><p>Finally, the appropriate irrigation area of this farm will be determined based on the measured amount of groundwater discharge at an existing well and the estimated irrigation water amount.</p>
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Pulp Mineral Content of Passion Fruit Germplasm Grown in Equador and Its Relationship with Fruit Qualiy Traits Reviewed
697 1 - 19 2022.03
Language：English Publishing type：Research paper (scientific journal)
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Phytochemical Composition and Antioxidant Activity of Passiflora spp. Germplasm Grown in Ecuador Reviewed
328 1 - 19 2022.01
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
Books and Other Publications 【 display / non-display 】
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熱帯作物学 3.3 花菜
篠原 卓（ Role： Joint author）
朝倉書店 2022.04
Language：Japanese Book type：Scholarly book
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園芸生産学 5.4 マメ類
篠原 卓（ Role： Joint author）
朝倉書店 2021.10
Language：Japanese Book type：Scholarly book
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小池 安比古, 高畑 健, 藤澤 弘幸, 河合 義隆 , 望岡 亮介 , 佐藤 明彦, 浅田 真一, 峯 洋子, 篠原 卓, 岡部 繭子, 元木 悟, 森 志郎, 窪田 聡（ Role： Contributor）
朝倉書店 2021 （ ISBN:9784254405514 ）
Language：Japanese Book type：Textbook, survey, introduction
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沙漠学事典 5章 砂漠の資源と経済活動、商品作物と付加価値化
篠原 卓（ Role： Joint author）
丸善出版 2020.07
Language：Japanese Book type：Scholarly book
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Genotyping Chapter 2 Microsatellite Capture Sequencing
（ Role： Joint author）
2017.11
Language：English Book type：Scholarly book
Misc 【 display / non-display 】
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Seed Vigour as a New Seed Quality Index and Its Utility
16 ( 1 ) 1 - 9 2009.07
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Scientific Research Funds Acquisition Results 【 display / non-display 】
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トランスクリプトーム解析によるパッションフルーツ成熟前落果発生機構解明
2021.06 - 2024.03
科学研究費補助金 基盤研究(C)
篠原 卓
Authorship：Principal investigator
パッションフルーツ（PF）は適熟となり生理的落果した果実を収穫する亜熱帯果樹である．近年，高品質な果実生産を目的とした国内栽培面積は増加しているが，酸度が高いまま落果する未成熟落果（PmFD）が課題となっている．これまでに果実からのエチレン放出量が非常に微量な時期にPmFDが発生すること，離層部のセルラーゼ活性は果実のエチレン放出量に応答しないことを報告してきた．本研究は，PFのPmFDの発生機構を明らかにするために，PF離層部におけるにPmFDに関わる細胞壁分解酵素をトランスクリプトーム解析により明らかにする試みである．
Other External Funds 【 display / non-display 】
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Development of rapid tests to predict germination and vigour and their potential for automation using image analysis
2019.09 - 2021.02
International Seed Testing Association
Dr.Sylvie Ducournau: GEVES, France. and Prof. Alison Powell: University of Aberdeen, UK, Dr Birte Boelt, Aarhus University, Denmark:
Authorship：Coinvestigator(s) Grant type：Competitive
There are demands for rapid methods of predicting germination and vigour. Published research has revealed that early radicle emergence (RE) counts and measurements of electrical conductivity (EC) predict normal germination (NG) and vigour of two Brassica
species. This project will 1) assess the potential of both tests to predict germination and vigour of commercial seed lots of five other Brassica species, and 2) examine the application of two systems of image analysis, RGB digital imaging (GEVES system) and multispectral analysis (Videometer), to assess radicle emergence. Seed lots covering a range of NG, above and below the commercially acceptable level, will be
used to identify 1) lots with unacceptable NG 2) differences in germination between lots with acceptable NG. Work on vigour will focus on lots with high acceptable NG; initial vigour assessments will be made using the Controlled Deterioration test and the
rate of, and final, emergence in field or glasshouse trials. Automated germination progress curves, produced using the GEVES digital imaging system will select times for RE counts that predict NG. RE counts using multispectral imaging will reveal if the
two image analysis systems give reproducible data and predict NG. The potential for measurements of the EC of seed soak water, taken at different times during 24 hours soaking to predict NG will also be examined. Both the EC and the selected RE counts will be compared to assessments of vigour to determine if they can be used as vigour tests. Members of the Germination and Vigour Committees will take part in
comparative tests on two of the species using the selected RE and EC assessments. These will use both manual assessments and image analysis systems to test their ability to assess NG and vigour and to provide data on repeatability and reproducibility for test validation.
Presentations 【 display / non-display 】
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エンドウ(Pisum sativum L.)の種子活力試験の有用性実証に関する研究
篠原卓、馬淵琴音、五十嵐大造
農業生産技術管理学会誌 26（別1）：29-30 於: 東京農業大学 農学部 厚木キャンパス） 2019.10
Event date： 2019.10
Language：English Presentation type：Oral presentation (general)
日本で市販されるエンドウ6品種各5ロット、計30ロットの種子を使用し、発芽率と種子活力試験（EC試験）を行った。また、これら30ロットの種子を10月、2月および3月に圃場に播種し、出芽率を計測した。EC試験の結果と出芽率と相関関係は有意で、発芽率よりも高い相関係数を示した。EC試験の結果と出芽率の相関関係は、圃場条件が劣悪な2月播種でも高かった。また、発芽率＞85％のロットのみを対象とした解析でも、EC試験の結果は圃場出芽率と高い相関を示した。活力試験としてのEC試験の有用性が実証された。
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The Use of Seed Vigour Test in Japan [日本における種子活力試験の利用状況] Invited International conference
Takashi Shinohara
Seed Vigour Seminer, International Seed Testing Association (ISTA) Annual Meeting 2018 （於: Sapporo） 2018.06
Event date： 2018.06
Language：English Presentation type：Oral presentation (invited, special)
我が国の農業の概要を説明し、比較的新しい品質指標である種子活力（Seed Vigour）が利用されている場面を研究成果とともに紹介した。また、今後、この品質指標が利用できると期待される場面について提案した。
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SSRマーカーによるジャボチカバの系統識別
篠原卓、本多俊介、吉田沙樹、五十嵐大造、田中啓介
熱帯農業研究10（別2）:71-72 （於: 香川大学 農学部） 2017.10
Event date： 2017.10
Language：English Presentation type：Oral presentation (general)
Microsatellite capture sequencing法により検出したSSR配列情報をもとにジャボチカバ３系統を識別するSSRマーカ―を開発した。９セットのプライマーのうち６セットでDNA増幅がみられ、実際に系統間に多型が検出されたのは３セットであった。そのうち１セット（Sonicは3つの系統を識別可能で最も識別能力の高いマーカーであると考えられた。
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主枝更新型せん定で整枝した赤系大粒ブドウ’クイーンニーナ’における結果枝への環状はく皮処理時期の違いが果粒肥大および着色に及ぼす影響 International conference
篠原卓、明井つかさ、吉田沙樹、五十嵐大造、石川一憲
熱帯農業研究10（別1）:27-28 （於: 日本大学 生物資源科学部 藤沢キャンパス） 2017.03
Event date： 2017.03
Language：English Presentation type：Oral presentation (general)
高温条件下での主枝更新型せん定で栽培したブドウ’クイーンニーナ’を用いて環状はく皮処理時期の違いが着色及び果実品質に及ぼす影響を明らかにしようとした。高温下での成熟により着色は低下したが,クイーンニーナには環状はく皮15日後処理で果粒サイズや果汁の糖度及び酸度に違いはないが,着色向上の効果があることが明らかになった.
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葉の形態形質の違いによるジャボチカバの系統識別および耐寒性の系統間差異に関する研究
篠原卓、藤崎絢帆、本多俊介、出沼大輔、吉田沙樹、五十嵐大造
熱帯農業研究10（別1）:29-30 （於: 日本大学 生物資源科学部 藤沢キャンパス） 2017.03
Event date： 2017.03
Language：English Presentation type：Oral presentation (general)
数年にわたる形態観察で,いくつかの形質が系統間で異なることを明らかにしてきた。これらの形態形質の違いを組み合わせることでジャボチカバの系統識別できるか検証した。大葉系統は葉の形態形質の違いにより他の2 系統との判別することは可能である。しかし、葉のサイズや葉色など環境条件で変化する量的形質であるため、小葉系統と中葉系統の識別を葉の形態形質の違いによって行うのは困難な場合があった。
Committee Memberships 【 display / non-display 】
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A Member of Seed Vigour Committee
2019.05
Committee type：Academic society
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日本熱帯農業学会 学会賞受賞候補者選考委員会 幹事
2014.04
Committee type：Academic society
Basic stance of industry-university cooperation 【 display / non-display 】
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問題解決型の研究を信条としております。積極的に取り組みたいと思います。