Papers - INOUE JUN
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Identification of Food-derived Bioactive Components with Physiological Effects
Inoue Jun
YAKUGAKU ZASSHI 145 ( 1 ) 23 - 28 2025.01
Language：Japanese Publisher：The Pharmaceutical Society of Japan
<p>Food-derived components with physiological effects have been attracting attention in recent years, and studies have comprehensively analyzed these components. In this study, we sought to identify food components with functional properties for the prevention and improvement of metabolic syndrome. We performed a luciferase reporter assay using fatty acid synthase (FAS) and low-density lipoprotein receptor (LDL) receptor gene promoters. Naturally occurring isothiocyanate sulforaphane impaired FAS promoter activity and reduced sterol regulatory element-binding protein (SREBP) target gene expression in human hepatoma Huh-7 cells. Sulforaphane reduced SREBP proteins by promoting the degradation of the SREBP precursor. Furthermore, we screened LDL receptor promoter effectors and observed that extract from sweet cherry peduncles induces LDL receptor gene promoter activity. Several analytical and chemical methods revealed that chrysin 7<i>O</i>-β-D-glucopyranoside in cherry peduncle extract stimulated LDL receptor gene promoter activity. Thus, this comprehensive search for components that alter the expression of genes associated with lipid metabolism led to the discovery of new functions of food components.</p>
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特集 脂質制御因子SREBPの新展開 ブロッコリー由来成分スルフォラファンによるSREBPタンパク質分解制御
井上 順
医学のあゆみ 291 ( 11 ) 1020 - 1023 2024.12
Publisher：医歯薬出版
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Identification of a novel target of sulforaphane: Sulforaphane binds to acyl-protein thioesterase 2 (APT2) and attenuates its palmitoylation. Reviewed International journal
Kodaka M, Kikuchi A, Kawahira K, Kamada H, Katsuta R, Ishigami K, Suzuki T, Yamamoto Y, and Inoue J
534 347 - 352 2024.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier
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HNF4α is required for Tkfc promoter activation by ChREBP. Reviewed
Tsukamoto R, Watanabe K, Kodaka M, Iwase M, Sakiyama H, Inoue Y, Suzuki T, Yamamoto Y, Shimizu M, Sato R, and Inoue J.
88 941 - 947 2024.07
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press
DOI： 10.1093/bbb/zbae067
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KODAKA Manami, INOUE Jun
KAGAKU TO SEIBUTSU 62 ( 3 ) 122 - 128 2024.03
Language：Japanese Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Chrysin 7-O-β-D-glucopyranoside increases hepatic low-density lipoprotein receptor expression through AMP-activated protein kinase activation. Reviewed International journal
Kaname N, Fujimaki T, Horikoshi S, Fujimura K, Kodaka M, Wakamori S, Katsuta R, Ishigami K, Suzuki T, Yamamoto Y, and Inoue J.
FEBS Open Bio 13 1447 - 1458 2023.08
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Effect of extracted rice protein hydrolysate on high-fat diet induced obesity in mice
Matsumoto Yu, Iwasaki Yu, Hosokawa Megumi, Suzuki Tsukasa, Inoue Jun, Shigemura Yasutaka, Takano Katsumi, Yamamoto Yuji
Nippon Shokuhin Kagaku Kogaku Kaishi 70 ( 5 ) 177 - 185 2023.05
Language：Japanese Publisher：Japanese Society for Food Science and Technology
<p>We investigated the effect of extracted rice protein hydrolysate (ERP), a byproduct of low-protein rice, for use as a therapeutic diet for kidney disease patients. The ERP was composed mostly of peptides and free amino acids. Administering ERP inhibited the body mass compared to obese model mice fed a high-fat diet. ERP administration also significantly increased and decreased fecal triglycerides and epididymal adipose tissue weight, respectively. Blood alanine aminotransferase levels decreased, indicating that hepatic inflammation was reduced. Also, a decrease in ceramide synthase 6, which is related to insulin resistance, was observed. Since ERP was composed mostly of peptides and free amino acids, we hypothesize that the observed effects were due to peptides and/or free amino acids. Based on the findings, ERP is a very useful anti-obesity food additive.</p>
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Current status and issues of functional food ingredients
Inoue Jun
Journal for the Integrated Study of Dietary Habits 34 ( 1 ) 13 - 17 2023
Language：Japanese Publisher：THE JAPAN ASSOCIATION FOR THE INTEGRATED STUDY OF DIETARY HABITS
<p> Food functions are categorized into primary functions as nutrients, secondary functions to enjoy food through taste and flavor, and tertiary functions to maintain health. Food functionality is widely recognized by general consumers. In recent years, the metabolism-improving effects of many food-derived ingredients have become clear, and their mechanisms have been vigorously analyzed.</p><p> The author has been conducting exploratory research using an evaluation system to identify food ingredients with anti-metabolic syndrome effects. The evaluation system is an experimental system constructed for easy screening of active ingredients. In this article, we will describe the evaluation system using a reporter assay with luciferase genes for FAS-Luc and LDLR-Luc. Using these evaluation systems, we found that xanthohumol and isoxanthohumol in hops and isothiocyanates, pungent components of cruciferous plants, inhibit SREBP activity, and that piperine, a pungent component of black pepper, and kaempferol, widely found in tea and many vegetables, increase LDLR expression and activity through enhancement of LDLR gene expression.</p>
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Sulforaphane suppresses the activity of sterol regulatory element-binding proteins (SREBPs) by promoting SREBP precursor degradation. Reviewed International journal
Miyata S, Kodaka M, Kikuchi A, Matsunaga Y, Shoji K, Kuan Y-C, Iwase M, Takeda K, Katsuta R, Ishigami K, Matsumoto Y, Suzuki T, Yamamoto Y, *Sato R, and *Inoue J.
Sci Rep 12 8715 2022.05
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Brown Rice Inhibits Development of Nonalcoholic Fatty Liver Disease in Obese Zucker (fa/fa) Rats by Increasing Lipid Oxidation Via Activation of Retinoic Acid Synthesis. Reviewed
Matsumoto Y, Fujita S, Yamagishi A, Shirai T, Maeda Y, Suzuki T, Kobayashi KI, Inoue J, and Yamamoto Y.
J Nutr 151 2705 - 2713 2021.09
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1093/jn/nxab188
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MIG12 is involved in the LXR activation-mediated induction of the polymerization of mammalian acetyl-CoA carboxylase. Reviewed
Izumi A, Hiraguchi H, Kodaka M, Ikeuchi E, Narita J, Kobayashi R, Matsumoto Y, Suzuki T, Yamamoto Y, Sato R, and Inoue J.
Biochem Biophys Res Commun 567 138 - 142 2021.08
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Study of Control Mechanism of Vitamin A Metabolism through its Specific Binding Proteins
Yamamoto Yuji, Matsumoto Yu, Suzuki Tsukasa, Inoue Jun
VITAMINS 95 ( 5-6 ) 257 - 265 2021.06
Language：Japanese Publisher：THE VITAMIN SOCIETY OF JAPAN
Vitamin A (retinol), which is also known as the retinoid, is one of fat-soluble vitamins. Vitamin A is required not only for the visual cycle but also for normal cell development and metabolism. Retinol is oxidatively metabolized to retinal and retinoic acid. Retinal is required for the visual cycle and retinoic acid regulates the expression of specific genes. In each case, the metabolism of vitamin A is regulated by specific proteins which are required for the function expression of vitamin A metabolites. To understand the regulatory mechanism of these vitamin A-mediated physiological actions, therefore, not only the concentration of vitamin A metabolites but also the behavior of specific proteins related to vitamin A such as enzymes, transport proteins, and nuclear receptors should be evaluated. This evaluation makes it possible to understand the complex reactions of vitamin A in vivo from the fluctuations of these proteins.<br>
In this comprehensive paper, we have shown both how these binding proteins regulate the action of vitamin A and a possibility that nutritional status affects retinol metabolism and actions by altering the expression of proteins related to vitamin A metabolism. -
Methionine controls insulin/mammalian target of rapamycin complex 1 activity by modulating tuberous sclerosis complex 2 stability. Reviewed
Gen S, Matsumoto Y, Suzuki T, Inoue J, and Yamamoto Y.
Biochem Biophys Res Commun 541 84 - 89 2021.02
Language：English Publishing type：Research paper (scientific journal)
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AMP-activated protein kinase regulates β-catenin protein synthesis by phosphorylating serine/arginine-rich splicing factor 9. Reviewed
Matsumoto E, Matsumoto Y, Inoue J, Yamamoto Y, and Suzuki T.
Biochem Biophys Res Commun 534 347 - 352 2021.01
Language：English Publishing type：Research paper (scientific journal)
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5．玄米摂取はレチノイン酸生合成を変えて非 アルコール性脂肪肝を改善する
山本 祐司, 松本 雄宇, 鈴木 司, 井上 順
ビタミン 95 ( 5-6 ) 302 2021
Language：Japanese Publisher：公益社団法人 日本ビタミン学会
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TSC2 methylation is required for its stabilization
Yamamoto Yuji, Suzuki Tsukasa, Inoue Jun
The Japanese Journal of SURGICAL METABOLISM and NUTRITION 55 ( 4 ) 57 2021
Language：Japanese Publisher：JAPANESE SOCIETY for SURGICAL METABOLISM and NUTRITION
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Stability of tuberous sclerosis complex 2 is controlled by methylation at R1457 and R1459. Reviewed
Gen S, Matsumoto Y, Kobayashi KI, Suzuki T, Inoue J, and Yamamoto Y
Sci Rep 10 21160 2020.12
Language：English Publishing type：Research paper (scientific journal)
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Niclosamide activates the AMP-activated protein kinase complex 1 containing the β2 subunit independently of AMP. Reviewed
Suzuki T, Kojima M, Matsumoto Y, Kobayashi KI, Inoue J, and Yamamoto Y
Biochem Biophys Res Commun 533 758 - 763 2020.11
Language：English Publishing type：Research paper (scientific journal)
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AMP-activated protein kinase regulates alternative pre-mRNA splicing by phosphorylation of SRSF1. Reviewed
Matsumoto E, Akiyama K, Saito T, Matsumoto Y, Kobayashi KI, Inoue J, Yamamoto Y, and *Suzuki T
Biochem J 477 2237 - 2248 2020.06
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1042/BCJ20190894
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Activation of Peroxisome Proliferator-Activated Receptor gamma/Small Heterodimer Partner Pathway Prevents High Fat Diet-Induced Obesity and Hepatic Steatosis in Sprague-Dawley Rats Fed Soybean Meal. Reviewed
Matsumoto Y, Ishimi Y, Suzuki T, Kobayashi K, Inoue J, and Yamamoto Y
J Nutr Biochem 75 108250. 2020.01
Language：English Publishing type：Research paper (scientific journal)
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機能性をもつ食品成分の探索と作用メカニズムの解明
井上 順
機能性食品と薬理栄養 13 1 - 6 2020
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Chrysin reduces the activity and protein level of mature forms of sterol regulatory element-binding proteins. Reviewed
Iwase M, Watanabe K, Shimizu M, Suzuki T, Yamamoto Y, Inoue J, and Sato R
Biosci. Biotechnol. Biochem. 83 1740 - 1746 2019.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Isoxanthohumol stimulates ubiquitin-proteasome-dependent degradation of precursor forms of sterol regulatory element-binding proteins. Reviewed
Inoue J, Miyata S, Shimizu M, and Sato R
Biosci. Biotechnol. Biochem. 82 1591 - 1598 2018.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
イソキサントフモールがSREBP前駆体の分解を促進することで、その活性を抑制することを報告した。
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The expression of Transmembrane Protein 100 is regulated by alterations in calcium signaling rather than endoplasmic reticulum stress. Reviewed
Kuboyama A, Sasaki T, Shimizu M, Inoue J, and Sato R
Biosci. Biotechnol. Biochem. 82 1377 - 1383 2018.08
Language：English Publishing type：Research paper (scientific journal)
Transmembrane protein 100の発現がカルシウムシグナルにより制御を受けることについて報告した。
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The exercise-inducible bile acid receptor Tgr5 improves skeletal muscle function in mice. Reviewed
Sasaki T, Kuboyama A, Mita M, Murata S, Shimizu M, Inoue J, Mori K, and Sato R
J. Biol. Chem. 293 10322 - 10332 2018.06
Language：English Publishing type：Research paper (scientific journal)
胆汁酸をリガンドとするGPCRであるTGR5が筋肉の機能を改善することを報告した。
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FGF21 Alleviates Hepatic Endoplasmic Reticulum Stress under Physiological Conditions. Reviewed
Maruyama R, Shimizu M, Hashidume T, Inoue J, Itoh N, and Sato R
J. Nutr. Sci. Vitaminol. (Tokyo). 64 200 - 208 2018.06
Language：English Publishing type：Research paper (scientific journal)
FGF19とFGF21遺伝子発現の栄養ストレスに対する応答について報告した。
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The flavonoid derivative 4'-nitro-6-hydroxyflavone suppresses the activity of HNF4α and stimulates the degradation of HNF4α protein through the activation of AMPK. Reviewed
Inoue, J., Ikeda, S., Kanayama, T., and Sato, R.
Biosci. Biotechnol. Biochem 81 1548 - 1552 2017.08
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
4'ニトロ6ヒドロキシフラバノンがAMPK活性化を介してHNF4αタンパク質の分解を促進することで、その活性を低下させることを報告した。
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Identification of key amino acid residues in the hTGR5-nomilin interaction and construction of its binding model. Reviewed
Sasaki, T., Mita, M., Ikari, N., Kuboyama, A., Hashimoto, S., Kaneko, T., Ishiguro, M., Shimizu, M., Inoue, J., and Sato, R.
PLoS ONE 12 e0179226. 2017.06
Language：English Publishing type：Research paper (scientific journal)
柑橘類に多く含まれるリモノイドに分類されるノミリンと胆汁酸受容体TGR5との結合に関与するTGR5アミノ酸残基について報告した。
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BCL11B gene heterozygosity causes weight loss accompanied by increased energy consumption, but not defective adipogenesis, in mice. Reviewed
Inoue, J., Ihara, Y., Tsukamoto, D., Yasumoto, K., Hashidume, T., Kamimura, K., Hirano, S., Shimizu, M., Kominami, R., and Sato, R.
Biosci. Biotechnol. Biochem. 81 922 - 930 2017.05
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
BCL11B遺伝子のヘテロ欠損マウスがエネルギー消費の上昇を伴う低体重を示すことについて報告した。
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Heat Shock Protein 90 Modulates Lipid Homeostasis by Regulating the Stability and Function of Sterol Regulatory Element-Binding Protein (SREBP) and SREBP Cleavage-Activating Protein. Reviewed
Kuan, Y-C., Hashidume, T., Shibata, T., Uchida, K., Shimizu, M., Inoue, J., and Sato, R.
J. Biol. Chem. 292 3106 - 3028 2017.02
Language：English Publishing type：Research paper (scientific journal)
HSP90がSCAPと結合すること、およびHSP90の抑制はSREBP/SCAP複合体の不安定化させることを報告した。
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Identification of BCL11B as a regulator of adipogenesis. Reviewed
Inoue, J., Ihara, Y., Tsukamoto, D., Yasumoto, K., Hashidume, T., Kamimura, K., Nakai, Y., Hirano, S., Shimizu, M., Kominami, R., and Sato, R.
Sci. Rep. 6 32750 2016.09
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
BCL11BがWnt/βcatenin経路の抑制作用を介して脂肪細胞分化を促進することを報告した。
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Single ingestion of soy β-conglycinin induces increased postprandial circulating FGF21 levels exerting beneficial health effects. Reviewed
Hashidume, T., Kato, A., Tanaka, T., Miyoshi, S., Itoh, N., Nakata, R., Inoue, H., Oikawa, A., Nakai, Y., Shimizu, M., Inoue, J., and Sato, R.
Sci. Rep. 6 28183 2016.06
Language：English Publishing type：Research paper (scientific journal)
大豆タンパク質であるβコングリシニンがFGF21発現を上昇させることを報告した。
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Searching for novel ATF4 target genes in human hepatoma cells by microarray analysis. Reviewed
Maruyama, R., Shimizu, M., Ishijima, T., Nakai, Y., Inoue, J., and Sato, R.
Biosci. Biotechnol. Biochem. 80 1149 - 1154 2016.06
Language：English Publishing type：Research paper (scientific journal)
マイクロアレイを用いてATF4の新規標的遺伝子を探索・同定を行った。
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Allyl isothiocyanate suppresses the proteolytic activation of sterol regulatory element-binding proteins and de novo fatty acid and cholesterol synthesis. Reviewed
Miyata, S., Inoue, J., Shimizu, M., and Sato, R.
Biosci. Biotechnol. Biochem. 80 1006 - 1011 2016.05
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
アリルイソチオシアネートがSREBP活性を抑制すること、および新規脂肪酸・コレステロール合成を抑制することを報告した。
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Fibroblast growth factor 21 induction by activating transcription factor 4 is regulated through three amino acid response elements in its promoter region. Reviewed
Maruyama, R., Shimizu, M., Li, J., Inoue, J., and Sato, R.
Biosci. Biotechnol. Biochem. 80 929 - 934 2016.05
Language：English Publishing type：Research paper (scientific journal)
ATF4がFGF21の発現を制御することを報告した。
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Kaempferol stimulates gene expression of low-density lipoprotein receptor through activation of Sp1 in cultured hepatocytes. Reviewed
Ochiai, A., Miyata, S., Iwase, M., Shimizu, M., Inoue, J., and Sato, R.
Sci. Rep. 6 24940 2016.04
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
血中のLDL-コレステロールレベルの上昇は、動脈硬化性疾患発症のリスクを高めることが知られている。LDL受容体は血中LDLを肝臓に取り込みクリアランスすることから、動脈硬化性疾患発症抑制の有望な標的であると考えられている。LDL受容体遺伝子発現を上昇させる食品由来成分の探索を行い、フラボノイドに分類されるケンフェロール（KPF）を見出した。KPFはERK活性化を介してSp1(Thr-453, Thr-739)のリン酸化を亢進し、それによりSp1のDNA結合能を上昇させることを示した。
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Perilipin2 plays a positive role in adipocytes during lipolysis by escaping proteasomal degradation. Reviewed
Takahashi, Y., Shinoda, A., Kamada, H., Shimizu, M., Inoue, J., and Sato, R.
Sci. Rep. 6 20975 2016.02
Language：English Publishing type：Research paper (scientific journal)
脂肪滴に局在するADRPタンパク質が、は脂肪滴に局在することで安定化することを報告した。
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Identification of the flavonoid luteolin as a repressor of the transcription factor hepatocyte nuclear factor 4α. Reviewed
Li, J., Inoue, J., Choi J.M., Nakamura, S., Yan, Z., Fushinobu, S., Kamada, H., Kato, H., Hashidume, T., Shimizu, M., and Sato, R.
J. Biol. Chem. 290 24021 - 24035 2015.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
肝臓や小腸からのリポタンパク質分泌に関与するMTP遺伝子発現を抑制する食品由来成分を探索し、フラボノイドに分類されるルテオリンを見出した。ルテオリンが転写因子HNF4αに結合しその活性を抑制すること、肥満マウスへのルテオリンの投与は血中VLDLコレステロール量を低下させることを示した。
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Xanthohumol improves diet-induced obesity and fatty liver by suppressing sterol regulatory element-binding protein (SREBP) activation. Reviewed
Miyata, S., Inoue, J., Shimizu, M., and Sato, R.
J. Biol. Chem. 290 20565 - 20579 2015.08
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
脂肪酸合成酵素遺伝子プロモーター活性を抑制する食品由来成分を探索し、ホップの成分であるキサントフモール(XN)を同定した。XNは転写因子SREBP-1の活性化を抑制すること、さらに肥満マウスへのXNの投与は抗肥満・抗脂肪肝作用を示した。SREBPの小胞-ゴルジ輸送に関与するSec23/24とXNが結合し、その結果としてSREBPのゴルジ体への輸送が阻害されることで、SREBP活性化（プロセッシング）が抑制されることを示した。
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Quercetin represses apolipoprotein B expression by inhibiting the transcriptional activity of C/EBPβ. Reviewed
Shimizu, M., Li, J., Inoue, J., and Sato, R.
PLoS ONE 10 e0121784 2015.04
Language：English Publishing type：Research paper (scientific journal)
ケルセチンがC/EBPβ活性を抑制することでApoB遺伝子発現を抑制することを報告した。
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Selective regulation of FGF19 and FGF21 expression by cellular and nutritional stress. Reviewed
Shimizu, M., Morimoto, H., Maruyama, R., Inoue, J., and Sato, R.
J. Nutr. Sci. Vitaminol. (Tokyo). 61 154 - 160 2015.04
Language：English Publishing type：Research paper (scientific journal)
FGF19とFGF21遺伝子発現の栄養ストレスに対する応答について報告した。
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Piperine induces hepatic low-density lipoprotein receptor expression through proteolytic activation of sterol regulatory element-binding proteins. Reviewed
Ochiai, A., Miyata, S., Shimizu, M., Inoue, J., and Sato, R.
PLoS ONE 10 e0139799 2015.01
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
ピペリンがSREBP活性化を介してLDL受容体発現および活性を上昇させることを報告した。
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Role of AMPK and PPARγ1 in exercise-induced lipoprotein lipase in skeletal muscle. Reviewed
Sasaki, T., Nakata, R., Inoue, H., Shimizu, M., Inoue, J., and Sato, R.
Am. J. Physiol. Endocrinol. Metab. 306 E1085 - E1092 2014.05
Language：English Publishing type：Research paper (scientific journal)
運動時に誘導されるリポプロテインリパーゼ遺伝子の発現制御について、AMPKとPPARγの関与について報告した。
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ATF6α stimulates cholesterogenic gene expression and de novo cholesterol synthesis. Reviewed
Maruyama, R., Kamoshida, Y, Shimizu, M., Inoue, J., and Sato, R.
Biosci. Biotechnol. Biochem. 77 1734 - 1738 2013.08
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
ATF6αがコレステロール合成系遺伝子の発現を上昇させ、新規コレステロール合成を亢進することを報告した。
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New insights into the activation of sterol regulatory element-binding proteins by proteolytic processing. Reviewed
Inoue, J. and Sato, R.
Biomol. Concepts 4 417 - 423 2013.08
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
SREBPの活性化機構についての最新の知見について報告した。
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Perilipin-mediated lipid droplet formation in adipocytes promotes sterol regulatory element-binding protein-1 processing and triacylglyceride accumulation. Reviewed
Takahashi, Y., Shinoda, A., Furuya, N., Harada, E., Arimura, N., Ichi, I., Fujiwara, Y., Inoue, J., and Sato, R.
PLoS ONE 8 e64605. 2013.05
Language：English Publishing type：Research paper (scientific journal)
脂肪細胞における脂肪滴形成は、SREBP-1活性化を介して脂肪合成促進、さらなる脂肪滴形成を亢進する正の循環システムを駆動させることを報告した。
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FGF19 (fibroblast growth factor 19) as a novel target gene for activating transcription factor 4 in response to endoplasmic reticulum stress. Reviewed
Shimizu, M., Li, J., Maruyama, R., Inoue, J., and Sato, R.
Biochem. J. 450 221 - 229 2013.02
Language：English Publishing type：Research paper (scientific journal)
小胞体ストレス誘導時にFGF19遺伝子が誘導されること、およびその誘導にATF4αが関与していることについて報告した。
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5-Aminoimidazole-4-carboxamide ribonucleoside stabilizes low density lipoprotein receptor mRNA in hepatocytes via ERK-dependent HuR binding to an AU-rich element. Reviewed
Yashiro, T., Nanmoku, M., Shimizu, M., Inoue, J., and Sato, R.
Atherosclerosis 226 95 - 101 2013.01
Language：English Publishing type：Research paper (scientific journal)
AICARがERK活性化に依存したHuRのLDL受容体mRNAへの結合を促進し、LDL受容体mRNAを安定化させることを報告した。
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4′-Hydroxyflavanone suppresses activation of sterol regulatory element-binding proteins and de novo lipid synthesis. Reviewed
Miyata, S., Inoue, J., Shimizu, M., and Sato, R.
FEBS Lett. 586 1778 - 1782 2012.06
Language：English Publishing type：Research paper (scientific journal)
4'ヒドロキシフラバノンがSREBPの活性を抑制すること、新規脂質合成を抑制することを報告した。
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Resveratrol increases the expression and activity of the low density lipoprotein receptor in hepatocytes by the proteolytic activation of the sterol regulatory element-binding proteins. Reviewed
Yashiro, T., Nanmoku, M., Shimizu, M., Inoue, J., and Sato, R.
Atherosclerosis 220 369 - 374 2012.02
Language：English Publishing type：Research paper (scientific journal)
レスベラトロールがSREBPの活性化を介してLDL受容体発現および活性を亢進することを報告した。
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Consumption of soy protein isolate reduces hepatic SREBP-1c and lipogenic gene expression in wild-type mice, but not in FXR-deficient mice. Reviewed
Hashidume, T., Sasaki, T., Inoue, J., and Sato, R.
Biosci. Biotechnol. Biochem. 75 1702 - 1707 2011.09
Language：English Publishing type：Research paper (scientific journal)
大豆タンパク質の摂取は肝臓におけるSREBP-1c発現を抑制するが、FXR欠損マウスではその抑制が起こらないことを報告した。
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Glutamine stimulates the gene expression and processing of sterol regulatory element-binding proteins, thereby increasing the expression of their target genes. Reviewed
Inoue, J., Ito, Y., Shimada, S., Satoh, S., Sasaki, T., Hashidume, T., Kamoshida, Y., Shimizu, M., and Sato, R.
FEBS J. 278 2739 - 2750 2011.08
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
培養細胞を用いて、培地中のグルタミンが転写因子SREBPの活性化を促進することを示した。この活性化は脂肪酸合成やコレステロール合成系遺伝子の発現を上昇させ、細胞増殖に必要な脂質成分の供給に関与している可能性を示した。
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Anti-obesity and anti-hyperglycemic effects of the dietary citrus limonoid nomilin in mice fed a high-fat diet. Reviewed
Ono, E., Inoue, J., Hashidume, T., Shimizu, M., and Sato, R.
Biochem. Biophys. Res. Commun. 410 677 - 681 2011.07
Language：English Publishing type：Research paper (scientific journal)
柑橘類に多く含まれるリモノイドに分類されるノミリンが胆汁酸受容体TGR5を活性化することを報告した。
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Stabilization of small heterodimer partner mRNA by grape seed procyanidins extract in cultured hepatocytes. Reviewed
Inoue, J., Tanaka, M., Nanmoku, M., Yashiro, T., and Sato, R.
Mol. Nutr. Food Res. 55 1052 - 1058 2011.07
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
ブドウ種子に含まれるGSPEが核内受容体SHPの発現を亢進することを示した。さらに、その発現上昇には、転写レベルでの発現上昇ではなく、SHP mRNAの安定化が関与することを示した。
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Identification of MIG12 as a mediator for stimulation of lipogenesis by LXR activation. Reviewed
Inoue, J., Yamasaki, K., Ikeuchi, E., Satoh, S., Fujiwara, Y., Nishimaki-Mogami, T., Shimizu, M., and Sato, R.
Mol. Endocrinol. 25 995 - 1005 2011.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
脂肪酸代謝は多くの転写因子による制御を受けているが、核内受容体LXRはその中でも中心的な役割を担っていることが知られている。本論文ではLXRの新規標的遺伝子としてMIG12を同定した。MIG12はLXR活性化時の新規脂肪酸合成の亢進に関与していることを示した。
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Chenodeoxycholic acid stabilization of LDL receptor mRNA depends on 3'-untranslated region and AU-rich element-binding protein. Reviewed
Yashiro, T., Yokoi, Y., Shimizu, M., Inoue, J., and Sato, R.
Biochem. Biophys. Res. Commun. 409 155 - 159 2011.06
Language：English Publishing type：Research paper (scientific journal)
胆汁酸であるCDCAによるLDL受容体mRNAの安定化には、3'UTRに存在するAUリッチ領域とそこに結合する因子が関与することを報告した。
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The gene expression of the myocardial lipid droplet protein is highly regulated by PPARgamma in adipocytes differentiated from MEFs or SVCs. Reviewed
Takahashi, Y., Shinoda, A., Inoue, J., and Sato, R.
Biochem. Biophys. Res. Commun. 399 209 - 214 2010.08
Language：English Publishing type：Research paper (scientific journal)
脂肪滴局在タンパク質であるMLDPの発現がMEFsやSVCsの脂肪細胞分化過程においてPPARγによって制御を受けることを報告した。
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Quercetin enhances VDR activity, leading to stimulation of its target gene expression in Caco-2 cells. Reviewed
Inoue, J., Choi, J.M., Yoshidomi, T., Yashiro, T., and Sato, R.
J. Nutr. Sci. Vitaminol. (Tokyo) 56 326 - 330 2010.01
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
ケルセチンがVDRを活性化することを報告した。
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The sterol-sensing ER membrane protein TRC8 hampers ER-to-Golgi transport of SREBP-2/SCAP and reduces SREBP-2 cleavage. Reviewed
Ozawa, N., Mori, K., and Sato, R., Inoue, J.
J. Biol. Chem. 284 28995 - 29004 2009.01
Language：English Publishing type：Research paper (scientific journal)
TRC8がSREBP-2/SCAP複合体のゴルジ体への輸送を阻害することでSREBP-2活性を低下させることを報告した。
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ApoC-III gene expression is sharply increased during adipogenesis and is augmented by retinoid X receptor (RXR) agonists. Reviewed
Takahashi, Y., Inoue, J., Kagechika, H., and Sato, R.
FEBS Lett. 583 493 - 497 2009.01
Language：English Publishing type：Research paper (scientific journal)
ApoCIII遺伝子発現が脂肪細胞分化過程で上昇すること、およびその上昇がRXR活性化剤の処理で増強されることを報告した。
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PPARalpha gene expression is up-regulated by LXR and PXR activators in the small intestine. Reviewed
Inoue, J., Satoh, S., Kita, M., Nakahara, M., Hachimura, S., Miyata, M., Nishimaki-Mogami T., and Sato, R.
Biochem. Biophys. Res. Commun. 371 675 - 678 2008.07
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
小腸においてPPARTαの発現がLXRおよびPXRによって制御されることを報告した。
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Growth factor-induced phosphorylation of SREBPs inhibits sumoylation, thereby stimulating the expression of their target genes, LDL uptake and lipid synthesis. Reviewed
Arito, M., Horiba, T., Hachimura, S., Inoue, J., and Sato, R.
J. Biol. Chem. 283 15224 - 15231 2008.05
Language：English Publishing type：Research paper (scientific journal)
増殖因子によるSREBPのリン酸化はSUMO化と拮抗し、SREBPを活性化することを報告した。
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Interaction between sterol regulatory element-binding proteins and liver receptor homolog-1 reciprocally suppresses their transcriptional activities. Reviewed
Inoue, J.
J. Biol. Chem. 282 10290 - 10298 2007.04
Language：English Publishing type：Research paper (scientific journal)
LRH-1とSREBPが結合すること、さらにその結合はそれぞれの転写活性を抑制することを報告した。
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Expression of TCRαβ partly rescues developmental arrest and apoptosis of αβ T cells in Bcl11b(-/-) mice. Reviewed
Inoue, J., Kanefuji, T., Okazuka, K., Watanabe, H., Mishima, Y., and Kominami, R.
J. Immunol. 176 5871 - 5879 2006.05
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
Bcl11bは我々が発見したがん抑制遺伝子である。Bcl11b欠損マウスとTCRαβ-Tgマウスを交配することにより、Bcl11b欠損による胸腺T細胞の分化抑制が一部レスキューされることを示した。Bcl11b欠損マウスにおけるT細胞分化抑制の一部がT cell receptor(TCR)のV(D)J遺伝子再構成不全に起因することを示した。
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Involvement of V(D)J recombinase in generation of intragenic deletions of Rit1/Bcl11b tumor suppressor gene in γ-ray-induced thymic lymphomas and in normal thymus of the mouse. Reviewed
Sakata, J., Inoue, J., Ohi, H., Kosugi-Okano, H., Mishima, Y., Hatakeyama, K., Niwa, O., and Kominami, R.
Carcinogenesis 25 1069 - 1075 2004.06
Language：English Publishing type：Research paper (scientific journal)
ガンマ線照射や正常胸腺におけるBCL11B遺伝子の欠損にはV(D)Jリコンビナーゼ活性が関与することを報告した。
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p53 prevents maturation of T cell development to the immature CD4(-)CD8(+) stage in Bcl11b(-/-) mice. Reviewed
Okazuka, K., Wakabayashi, Y., Kashihara, M., and Inoue, J., Sato, T., Yokoyama, M., Aizawa, S., Aizawa, Y., Mishima Y., and Kominami, R.
Biochem. Biophys. Res. Commun. 328 545 - 549 2004.03
Language：English Publishing type：Research paper (scientific journal)
p53がBCL11B欠損マウスにおけるT細胞の分化に関与することを報告した。
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Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo. Reviewed
Arlotta, P., Molyneaux, B.J., Chen, J., Inoue, J., Kominami, R., and Macklis, J.D.
Neuron 45 207 - 221 2004.01
Language：English Publishing type：Research paper (scientific journal)
BCL11Bが皮質脊髄運動ニューロンの発達に関与することを報告した。
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Sterol regulatory element-binding protein-2 interacts with hepatocyte nuclear factor-4 to enhance sterol isomerase gene expression in hepatocytes. Reviewed
Misawa, K., Horiba, T., Arimura, N., Hirano, Y., Inoue, J., Emoto, N., Shimano, H., Shimizu, M., and Sato, R.
J. Biol. Chem. 278 36176 - 36182 2003.09
Language：English Publishing type：Research paper (scientific journal)
SREBP-2とHNF4αが協調的に作用することでステロールイソメラーゼ遺伝子の発現が促進されることを報告した。
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Bcl11b is required for differentiation and survival of αβ T lymphocytes. Reviewed
Wakabayashi, Y., Watanabe, H., Inoue, J., Takeda, N., Sakata, J., Mishima, Y., Hitomi, J., Yamamoto, T., Utsuyama, M., Niwa, O., Aizawa, S., and Kominami, R.
Nat. Immunol. 4 533 - 539 2003.06
Language：English Publishing type：Research paper (scientific journal)
Bcl11b欠損によって、胸腺T細胞分化が初期段階で停止することを示し、Bcl11bが胸腺T細胞の分化に必須な因子であることを明らかにした。Bcl11b欠損マウスでは、T cell receptor(TCR)のV(D)J遺伝子再構成がうまくいかず、その結果として分化が抑制される可能性を示した。
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Homozygous deletions and point mutations of the Rit1/Bcl11b gene in γ-ray induced mouse thymic lymphomas. Reviewed
Wakabayashi, Y., Inoue, J., Takahashi, Y., Matsuki, A., Kosugi-Okano, H., Shinbo, T., Mishima, Y., Niwa, O., and Kominami, R.
Biochem. Biophys. Res. Commun. 301 598 - 603 2003.02
Language：English Publishing type：Research paper (scientific journal)
ガンマ線誘導性の胸腺リンパ腫を対象としたポジショナルクローニングによりBCL11Bを新規がん抑制遺伝子として同定した。
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Proteolytic activation of SREBPs during adipocyte differentiation. Reviewed
Inoue, J., Kumagai, H., Terada, T., Maeda, M., Shimizu, M., and Sato, R.
Biochem. Biophys. Res. Commun. 283 1157 - 1161 2001.05
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
脂肪細胞分化時にSREBP-1がプロセッシングレベルで活性化されることを間接的に証明した。SREBPのプロセッシングによる活性化は、細胞内のコレステロールレベルにより制御されることが報告されていたが、本論文は脂肪細胞分化時におけるSREBPプロセッシングの、新たな制御機構の存在を示した。
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Transcriptional regulation of the ATP citrate-lyase gene by sterol regulatory element-binding proteins. Reviewed
Sato, R., Okamoto, A., Inoue, J., Miyamoto, W., Sakai, Y., Emoto, N., Shimano, H., and Maeda, M.
J. Biol. Chem. 275 12497 - 12502 2000.04
Language：English Publishing type：Research paper (scientific journal)
ACLがSREBPの新規な標的遺伝子であることを示した。ACL遺伝子プロモーター領域の解析を行い、その制御機構について報告した。
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脂質代謝の制御を担う転写因子SREBPに関する研究
井上順
2000.03
Language：Japanese Publishing type：Doctoral thesis
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Sterol regulatory element-binding protein negatively regulates microsomal triglyceride transfer protein gene transcription. Reviewed
Sato, R., Miyamoto, W., Inoue, J., Terada, T., Imanaka, T., and Maeda, M.
J. Biol. Chem. 274 24714 - 24720 1999.08
Language：English Publishing type：Research paper (scientific journal)
MTP遺伝子がSREBPによって負に制御を受けることを報告した。
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A novel splicing isoform of mouse sterol regulatory element-binding protein-1 (SREBP-1). Reviewed
Inoue, J., and Sato, R.
Biosci. Biotechnol. Biochem. 3 243 - 245 1999.01
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
SREBP-1の新規スプライシングアイソフォームについて報告した。
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Multiple DNA elements for sterol regulatory element-binding protein and NF-Y are responsible for sterol-regulated transcription of the genes for human 3-hydroxy-3-methylglutaryl coenzyme A synthase and squalene synthase. Reviewed
Inoue, J., Sato, R., and Maeda, M.
J. Biochem. (Tokyo) 123 1191 - 1198 1998.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
HNG-CoA合成酵素とスクアレン合成酵素遺伝子の発現がSREBPとNF-Yによって協調的な制御されることを報告した。
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Sterol-dependent transcription regulation of sterol regulatory element-binding protein-2. Reviewed
Sato, R., Inoue, J., Kawabe, Y., Kodama, T., Takano, T., and Maeda, M.
J. Biol. Chem. 271 26461 - 26464 1996.10
Language：English Publishing type：Research paper (scientific journal)
転写因子SREBP-2が自身のプロモーター領域に結合し、その発現を亢進することを示した。本論文は、SREBPの活性制御が細胞内コレステロールレベルに応じたプロセッシングのみではなく、自身の転写を活性化するセルフレギュレーションによっても行われていることを示した。