Papers - INOUE JUN
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Identification of Food-derived Bioactive Components with Physiological Effects
Inoue Jun
YAKUGAKU ZASSHI 145 ( 1 ) 23 - 28 2025.01
Language：Japanese Publisher：The Pharmaceutical Society of Japan
<p>Food-derived components with physiological effects have been attracting attention in recent years, and studies have comprehensively analyzed these components. In this study, we sought to identify food components with functional properties for the prevention and improvement of metabolic syndrome. We performed a luciferase reporter assay using fatty acid synthase (FAS) and low-density lipoprotein receptor (LDL) receptor gene promoters. Naturally occurring isothiocyanate sulforaphane impaired FAS promoter activity and reduced sterol regulatory element-binding protein (SREBP) target gene expression in human hepatoma Huh-7 cells. Sulforaphane reduced SREBP proteins by promoting the degradation of the SREBP precursor. Furthermore, we screened LDL receptor promoter effectors and observed that extract from sweet cherry peduncles induces LDL receptor gene promoter activity. Several analytical and chemical methods revealed that chrysin 7<i>O</i>-β-D-glucopyranoside in cherry peduncle extract stimulated LDL receptor gene promoter activity. Thus, this comprehensive search for components that alter the expression of genes associated with lipid metabolism led to the discovery of new functions of food components.</p>
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特集 脂質制御因子SREBPの新展開 ブロッコリー由来成分スルフォラファンによるSREBPタンパク質分解制御
井上 順
医学のあゆみ 291 ( 11 ) 1020 - 1023 2024.12
Publisher：医歯薬出版
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Identification of a novel target of sulforaphane: Sulforaphane binds to acyl-protein thioesterase 2 (APT2) and attenuates its palmitoylation. Reviewed International journal
Kodaka M, Kikuchi A, Kawahira K, Kamada H, Katsuta R, Ishigami K, Suzuki T, Yamamoto Y, and Inoue J
534 347 - 352 2024.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier
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HNF4α is required for Tkfc promoter activation by ChREBP. Reviewed
Tsukamoto R, Watanabe K, Kodaka M, Iwase M, Sakiyama H, Inoue Y, Suzuki T, Yamamoto Y, Shimizu M, Sato R, and Inoue J.
88 941 - 947 2024.07
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press
DOI： 10.1093/bbb/zbae067
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KODAKA Manami, INOUE Jun
KAGAKU TO SEIBUTSU 62 ( 3 ) 122 - 128 2024.03
Language：Japanese Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Chrysin 7-O-β-D-glucopyranoside increases hepatic low-density lipoprotein receptor expression through AMP-activated protein kinase activation. Reviewed International journal
Kaname N, Fujimaki T, Horikoshi S, Fujimura K, Kodaka M, Wakamori S, Katsuta R, Ishigami K, Suzuki T, Yamamoto Y, and Inoue J.
FEBS Open Bio 13 1447 - 1458 2023.08
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Effect of extracted rice protein hydrolysate on high-fat diet induced obesity in mice
Matsumoto Yu, Iwasaki Yu, Hosokawa Megumi, Suzuki Tsukasa, Inoue Jun, Shigemura Yasutaka, Takano Katsumi, Yamamoto Yuji
Nippon Shokuhin Kagaku Kogaku Kaishi 70 ( 5 ) 177 - 185 2023.05
Language：Japanese Publisher：Japanese Society for Food Science and Technology
<p>We investigated the effect of extracted rice protein hydrolysate (ERP), a byproduct of low-protein rice, for use as a therapeutic diet for kidney disease patients. The ERP was composed mostly of peptides and free amino acids. Administering ERP inhibited the body mass compared to obese model mice fed a high-fat diet. ERP administration also significantly increased and decreased fecal triglycerides and epididymal adipose tissue weight, respectively. Blood alanine aminotransferase levels decreased, indicating that hepatic inflammation was reduced. Also, a decrease in ceramide synthase 6, which is related to insulin resistance, was observed. Since ERP was composed mostly of peptides and free amino acids, we hypothesize that the observed effects were due to peptides and/or free amino acids. Based on the findings, ERP is a very useful anti-obesity food additive.</p>
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Current status and issues of functional food ingredients
Inoue Jun
Journal for the Integrated Study of Dietary Habits 34 ( 1 ) 13 - 17 2023
Language：Japanese Publisher：THE JAPAN ASSOCIATION FOR THE INTEGRATED STUDY OF DIETARY HABITS
<p> Food functions are categorized into primary functions as nutrients, secondary functions to enjoy food through taste and flavor, and tertiary functions to maintain health. Food functionality is widely recognized by general consumers. In recent years, the metabolism-improving effects of many food-derived ingredients have become clear, and their mechanisms have been vigorously analyzed.</p><p> The author has been conducting exploratory research using an evaluation system to identify food ingredients with anti-metabolic syndrome effects. The evaluation system is an experimental system constructed for easy screening of active ingredients. In this article, we will describe the evaluation system using a reporter assay with luciferase genes for FAS-Luc and LDLR-Luc. Using these evaluation systems, we found that xanthohumol and isoxanthohumol in hops and isothiocyanates, pungent components of cruciferous plants, inhibit SREBP activity, and that piperine, a pungent component of black pepper, and kaempferol, widely found in tea and many vegetables, increase LDLR expression and activity through enhancement of LDLR gene expression.</p>
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Sulforaphane suppresses the activity of sterol regulatory element-binding proteins (SREBPs) by promoting SREBP precursor degradation. Reviewed International journal
Miyata S, Kodaka M, Kikuchi A, Matsunaga Y, Shoji K, Kuan Y-C, Iwase M, Takeda K, Katsuta R, Ishigami K, Matsumoto Y, Suzuki T, Yamamoto Y, *Sato R, and *Inoue J.
Sci Rep 12 8715 2022.05
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Brown Rice Inhibits Development of Nonalcoholic Fatty Liver Disease in Obese Zucker (fa/fa) Rats by Increasing Lipid Oxidation Via Activation of Retinoic Acid Synthesis. Reviewed
Matsumoto Y, Fujita S, Yamagishi A, Shirai T, Maeda Y, Suzuki T, Kobayashi KI, Inoue J, and Yamamoto Y.
J Nutr 151 2705 - 2713 2021.09
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1093/jn/nxab188
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MIG12 is involved in the LXR activation-mediated induction of the polymerization of mammalian acetyl-CoA carboxylase. Reviewed
Izumi A, Hiraguchi H, Kodaka M, Ikeuchi E, Narita J, Kobayashi R, Matsumoto Y, Suzuki T, Yamamoto Y, Sato R, and Inoue J.
Biochem Biophys Res Commun 567 138 - 142 2021.08
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Study of Control Mechanism of Vitamin A Metabolism through its Specific Binding Proteins
Yamamoto Yuji, Matsumoto Yu, Suzuki Tsukasa, Inoue Jun
VITAMINS 95 ( 5-6 ) 257 - 265 2021.06
Language：Japanese Publisher：THE VITAMIN SOCIETY OF JAPAN
Vitamin A (retinol), which is also known as the retinoid, is one of fat-soluble vitamins. Vitamin A is required not only for the visual cycle but also for normal cell development and metabolism. Retinol is oxidatively metabolized to retinal and retinoic acid. Retinal is required for the visual cycle and retinoic acid regulates the expression of specific genes. In each case, the metabolism of vitamin A is regulated by specific proteins which are required for the function expression of vitamin A metabolites. To understand the regulatory mechanism of these vitamin A-mediated physiological actions, therefore, not only the concentration of vitamin A metabolites but also the behavior of specific proteins related to vitamin A such as enzymes, transport proteins, and nuclear receptors should be evaluated. This evaluation makes it possible to understand the complex reactions of vitamin A in vivo from the fluctuations of these proteins.<br>
In this comprehensive paper, we have shown both how these binding proteins regulate the action of vitamin A and a possibility that nutritional status affects retinol metabolism and actions by altering the expression of proteins related to vitamin A metabolism. -
Methionine controls insulin/mammalian target of rapamycin complex 1 activity by modulating tuberous sclerosis complex 2 stability. Reviewed
Gen S, Matsumoto Y, Suzuki T, Inoue J, and Yamamoto Y.
Biochem Biophys Res Commun 541 84 - 89 2021.02
Language：English Publishing type：Research paper (scientific journal)
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AMP-activated protein kinase regulates β-catenin protein synthesis by phosphorylating serine/arginine-rich splicing factor 9. Reviewed
Matsumoto E, Matsumoto Y, Inoue J, Yamamoto Y, and Suzuki T.
Biochem Biophys Res Commun 534 347 - 352 2021.01
Language：English Publishing type：Research paper (scientific journal)
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5．玄米摂取はレチノイン酸生合成を変えて非 アルコール性脂肪肝を改善する
山本 祐司, 松本 雄宇, 鈴木 司, 井上 順
ビタミン 95 ( 5-6 ) 302 2021
Language：Japanese Publisher：公益社団法人 日本ビタミン学会
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TSC2 methylation is required for its stabilization
Yamamoto Yuji, Suzuki Tsukasa, Inoue Jun
The Japanese Journal of SURGICAL METABOLISM and NUTRITION 55 ( 4 ) 57 2021
Language：Japanese Publisher：JAPANESE SOCIETY for SURGICAL METABOLISM and NUTRITION
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Stability of tuberous sclerosis complex 2 is controlled by methylation at R1457 and R1459. Reviewed
Gen S, Matsumoto Y, Kobayashi KI, Suzuki T, Inoue J, and Yamamoto Y
Sci Rep 10 21160 2020.12
Language：English Publishing type：Research paper (scientific journal)
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Niclosamide activates the AMP-activated protein kinase complex 1 containing the β2 subunit independently of AMP. Reviewed
Suzuki T, Kojima M, Matsumoto Y, Kobayashi KI, Inoue J, and Yamamoto Y
Biochem Biophys Res Commun 533 758 - 763 2020.11
Language：English Publishing type：Research paper (scientific journal)
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AMP-activated protein kinase regulates alternative pre-mRNA splicing by phosphorylation of SRSF1. Reviewed
Matsumoto E, Akiyama K, Saito T, Matsumoto Y, Kobayashi KI, Inoue J, Yamamoto Y, and *Suzuki T
Biochem J 477 2237 - 2248 2020.06
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1042/BCJ20190894
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Activation of Peroxisome Proliferator-Activated Receptor gamma/Small Heterodimer Partner Pathway Prevents High Fat Diet-Induced Obesity and Hepatic Steatosis in Sprague-Dawley Rats Fed Soybean Meal. Reviewed
Matsumoto Y, Ishimi Y, Suzuki T, Kobayashi K, Inoue J, and Yamamoto Y
J Nutr Biochem 75 108250. 2020.01
Language：English Publishing type：Research paper (scientific journal)