論文 - 庫本 高志
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The rat Downunder (<i>Du</i>) coat color mutation is associated with eye anomalies and embryonic lethality and maps to a 3.9-Mb region on chromosome 3
HIEU Hoang Trung, TANAKA Miyuu, KUWAMURA Mitsuru, MASHIMO Tomoji, SERIKAWA Tadao, KURAMOTO Takashi
Experimental Animals advpub ( 0 ) 2022年
記述言語：英語 出版者・発行元：Japanese Association for Laboratory Animal Science
<p>Rodent coat color genes have been studied as a bioresource to understand developmental and cellular processes. The Downunder rat is a fancy variety with a marking on its belly that runs from the neck to the breech and appears to mirror the dorsal hooded marking. Here, we established a congenic strain carrying the Downunder (<i>Du</i>) gene in an F344 genetic background. In addition to the ventral marking, <i>Du</i>/+ rats exhibit anophthalmia or microphthalmia with incomplete penetrance. <i>Du</i>/<i>Du</i> embryos die in the early stages of organogenesis. Genetic linkage analysis mapped the <i>Du</i> gene to rat chromosome 3 and haplotype mapping with congenic rats localized the <i>Du</i> locus to a 3.9-Mb region. The <i>Du</i> locus includes two functional genes, glycosyltransferase-like domain-containing 1 (<i>Gtdc1</i>) and zinc finger E-box binding homeobox 2 (<i>Zeb2</i>). Although we found no functional variation within any of <i>Zeb2</i>’s exons or intron-exon boundaries, <i>Zeb2</i> mRNA levels were significantly lower in <i>Du</i>/+ rats compared with wild-type rats. It is known that melanocyte-specific <i>Zeb2</i> deletion results in the congenital loss of hair pigmentation in mice. Taken together, our results indicate that the <i>Du</i> mutation exerts pleiotropic effects on hair pigmentation, eye morphology, and development. Moreover, the <i>Zeb2</i> gene is a strong candidate for the <i>Du</i> mutation.</p>
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Positional cloning of rat mutant genes reveals new functions of these genes
KURAMOTO Takashi
Experimental Animals advpub ( 0 ) 2022年
記述言語：英語 出版者・発行元：Japanese Association for Laboratory Animal Science
<p>The laboratory rat (<i>Rattus norvegicus</i>) is a key model organism for biomedical research. Rats can be subjected to strict genetic and environmental controls. The rat’s large body size is suitable for both surgical operations and repeated measurements of physiological parameters. These advantages have led to the development of numerous rat models for genetic diseases. Forward genetics is a proven approach for identifying the causative genes of these disease models but requires genome resources including genetic markers and genome sequences. Over the last few decades, rat genome resources have been developed and deposited in bioresource centers, which have enabled us to perform positional cloning in rats. To date, more than 100 disease-related genes have been identified by positional cloning. Since some disease models are more accessible in rats than mice, the identification of causative genes in these models has sometimes led to the discovery of novel functions of genes. As before, various mutant rats are also expected to be discovered and developed as disease models in the future. Thus, the forward genetics continues to be an important approach to find genes involved in disease phenotypes in rats. In this review, I provide an overview the development of rat genome resources and describe examples of positional cloning in rats in which novel gene functions have been identified.</p>
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Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats 査読あり 国際誌
Hoang Trung H, Yoshihara T, Nakao A, Hayashida K, Hirata Y, Shirasuna K, Kuwamura M, Nakagawa Y, Kaneko T, Mori Y, Asano M, Kuramoto T
Sci Rep 11 2039 2021年02月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Genetic polymorphism of bovine beta-casein gene in Japanese dairy farm herds 査読あり
Yamada A, Sugimura M, Kuramoto T
Anim Sci J 92 ( 1 ) e13644 2021年
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1111/asj.13644.
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PHF24 is expressed in the inhibitory interneurons in rats 査読あり
Numakura Y, Uemura R, Tanaka M, Izawa T, Yamate J, Kuramoto T, Kaneko T, Mashimo T, Yamamoto T, Serikawa T, Kuwamura M
Exp Anim 70 ( 1 ) 137 - 143 2021年
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Involvement of NMDA receptors in tremor expression in Aspa/Hcn1 double-knockout rats 査読あり 国際誌
Nishitani A, Nagayoshi H, Takenaka S, Asano M, Shimizu S, Ohno Y, Kuramoto T
Exp Anim 64 ( 9 ) 388 - 394 2020年
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本実験動物学会
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Long terminal repeat insertion in Kit causes unilateral renal agenesis in rats 査読あり 国際誌
Ara S, Miyazaki A, Hoang Trung H, Yokoe M, Nakagwa Y, Kaneko T, Kuramoto T
Tranlat Regulat Sci 2 ( 1 ) 30 - 35 2020年
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Muscle weakness and impaired motor coordination in Hcn1-deficient rats 査読あり 国際誌
Nishitani A, Yoshihara T, Tanaka M, Kuwamura M, Asano M, Tsubota Y, Kuramoto T
Exp Anim 64 ( 9 ) 388 - 394 2020年
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本実験動物学会
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Increased seizure sensitivity defects and cognitive impairment in PHD finger protein (Phf24)-null rats 査読あり 国際誌
Serikawa T, Kunisawa N, Shimizu S, Kato M, Iha A, Kinboshi M, Nishikawa H, Shirakawa Y, Voigt B, Nakanishi S, Kuramoto T, Kaneko T, Yamamoto T, Mashimo T, Sasa M, Ohno Y
Behav Brain Res 269 111922 2019年09月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Downregulation of aspartoacylase during the progression of myelin breakdown in the dmy mutant rat with mitochondrial magnesium channel MRS2 defect 査読あり 国際誌
Kuwamura M, Tanimura S, Hasegawa Y, Hoshiai R, Moriyama Y, Tanaka M, Takenaka S, Nagayoshi H, Izawa T, Yamate J, Kuramoto T, Serikawa T
Brain Res 1718 169 - 175 2019年09月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Anti-seizure effect and neuronal activity change in the genetic-epileptic model rat with acute and chronic vagus nerve stimulation. 査読あり 国際誌
Katagiri M, Iida K, Ishihara K, Nair D, Harada K, Kagawa K, Seyama G, Hashizume A, Kuramoto T, Hanaya R, Arita K, Kurisu K
Epilepsy Res 155 106159 2019年09月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effects of exposure to mild hyperbaric oxygen on DSS-induced colonic inflammation and diarrhea in rats 査読あり 国際誌
Takemura A, Egawa T, Tanaka T, Kuramoto T, Hayashi T, Ishihara A
J Inflammation 12 293 - 299 2019年
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.2147/JIR.S220586
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Loss of HCN1 subunits causes absence epilepsy in rats 査読あり 国際誌
Nishitani A, Kunisawa N, Sugimura T, Sato K, Yoshida Y, Suzuki T, Sakuma T, Yamamoto T, Asano M, Saito Y, Ohno Y, Kuramoto T
Brain Res 1706 209 - 217 2018年05月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Inhibitory effects of pentoxifylline on inflammation-related tumorigenesis in rat colon 査読あり 国際誌
Shirakami Y, Kochi T, Kubota M, Sakai H, Ibuka T, Yoshimi K, Kuramoto T, Tanaka T, Shimizu M, Seishima M
Oncotarget 9 ( 74 ) 33972 - 33981 2018年
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Rat polyomavirus 2 infection in a colony of X-linked severe combined immunodeficiency rats in Japan 査読あり 国際誌
Tanaka M, Kuramochi M, Nakanishi S, Kuwamura M, Kuramoto T
J Vet Med Sci 80 ( 9 ) 1400 - 1406 2018年
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1292/jvms.18-0107
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Genetic profiling of two phenotypically distinct outbred rats derived from a colony of the Zucker fatty rats maintained at Tokyo Medical University 査読あり
Nakanishi S, Kuramoto T, Kashiwazaki N, Yokoi N
Exp Anim 66 91 - 98 2017年05月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Zuckerファッティラットの遺伝プロファイルを明らかにした。
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A deletion in the intergenic region upstream of Ednrb causes head spot in the rat strain KFRS4/Kyo 査読あり
Yoshihara M, Sato T, Saito D, Ohara O, Kuramoto T, Suyama M
BMC Genet 18 29 - 29 2017年03月
記述言語：英語 掲載種別：研究論文（学術雑誌）
頭部白斑変異の原因遺伝子がエンドセリン受容体Bの欠失であることをしめした。
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Rat genome and model resources
Shimoyama M, Smith JR, Bryda E, Kuramoto T, Saba L, Dwinell M
ILAR J 58 ( 1 ) 42 - 58 2017年
記述言語：英語 掲載種別：研究論文（学術雑誌）
ラットリソースの現状を報告した。
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A deletion in the intergenic region upstream of Ednrb causes head spot in the rat strain KFRS4/Kyo
Yoshihara M, Sato T, Saito D, Ohara O, Kuramoto T, Suyama M
BMC Genetics 18 29 - 29 2017年
記述言語：英語 掲載種別：研究論文（学術雑誌）
ラット頭部白斑変異の原因が、エンドセリン受容体B遺伝子のエンハンサー領域の欠失であることを示した。
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Tremor dominant Kyoto (Trdk) rats carry a missense mutation in the gene encoding the SK2 subunit of small-conductance Ca2+-activated K+ channel
Kuramoto T, Yokoe M, Kunisawa N, Ohashi K, Miyake T, Higuchi Y, Yoshimi K, MashimoT, Tanaka M, Kuwamura M, Kaneko S, Shimizu S, Serikawa T, Ohno Y
Brain Research 1676 38 - 45 2017年
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
振戦を示す変異ラットの原因遺伝子が、KCNN2遺伝子であることを示した。