Updated on 2023/05/23


IGIMI Sizunobu




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Degree 【 display / non-display

  • 農学博士 ( 1989.03   東京大学 )

Employment Record in Research 【 display / non-display

  • Tokyo University of Agriculture   Nodai Research Institute   Professor


  • Tokyo University of Agriculture   Faculty of Applied Bio-Science   Department of Applied Biology and Chemistry   Professor

    2016.04 - 2018.03

  • Tokyo University of Agriculture   Faculty of Applied Bio-Science   Department of Agricultural Chemistry   Professor

    2018.04 - 2023.03

Papers 【 display / non-display

  • The Importance of the Validated Microbiological Test Method and Direction of Microbial Testing under HACCP Control

    Igimi Shizunobu

    Foods & Food Ingredients Journal of Japan   227 ( 1 )   004 - 009   2022

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    Language:Japanese   Publisher:Editorial Board of Foods & Food Ingredients Journal of Japan  

    The importance of the validated microbiological test method and how to select the test method based on the fitness for purpose will be explained. Based on this, I will show how to select a microbial test method after the transition to process control such as the HACCP (Hazard Analysis and Critical Control Point), and how to utilize microbial test for risk management.

    DOI: 10.34457/ffij.227.1_004

    CiNii Articles

  • Studies on Epidemiology of Microorganisms Related to Human Health Disorders, and Their Control

    Japanese Journal of Food Microbiology   38 ( 2 )   53 - 66   2021.06

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    Language:Japanese   Publisher:Japanese Society of Food Microbiology  

    DOI: 10.5803/jsfm.38.53

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  • Research on the safety of genetically modified foods led to the development of oral mucosal vaccines of Lactic Acid Bacteria.

    Igimi Shizunobu

    Japanese Journal of Lactic Acid Bacteria   32 ( 1 )   7 - 14   2021.03

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    Language:Japanese   Publisher:Japan Society for Lactic Acid Bacteria  

    <p>Research on the safety of genetically modified foods began in 1989 by forming a research group with researchers from private dairy companies. The group contributed to the establishment of the Japan Society for Lactic Acid Bacteria academically and to the development of international guidelines on the safety of genetically modified foods administratively. The group needed a recombinant lactic acid bacterium as a model for safety evaluation, which was later expanded to recombinant vaccine development research.</p><p>Based on the previous studies of vaccination, we are recognized that the administration of a vaccine through the infection route of a pathogen is highly effective for vaccination. According to the concept, oral vaccination is the best strategy to induce both local mucosal and systemic immunity against mucosal infective type pathogens. Among several candidates of vaccine agents, <i>Lactobacillus</i> strains are particularly attractive as the antigen delivery vehicles because of the safeness and the adjuvanticity. we constructed recombinant Lactic Acid Bacteria expressing many kinds of protective antigens. Most successful vaccine has been established oral heat-killed vaccine for human papillomavirus</p><p>(HPV). As a result, high levels of HPV E7 specific cytotoxic T lymphocyte (CTL) were induced from the immunized both mice and human. Currently, human clinical trials are underway using this vaccine.</p>

    DOI: 10.4109/jslab.32.7

  • Construction of an <i>in vitro</i> human M cell model using RANKL

    Ono Tatsuya, Masuda Kazuya, Kajikawa Akinobu, Yokota Kenji, Igimi Shizunobu

    Proceedings for Annual Meeting of The Japanese Pharmacological Society   94 ( 0 )   1-O-G2-4   2021

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    Language:Japanese   Publisher:Japanese Pharmacological Society  

    <p>Intestinal immunity is triggered mainly by M cells existing in the intestinal tract by uptake antigens to gut associated lymphoid tissue (GALT). Recently, it has been attracting attention as a target of oral vaccine. However, primary culture is difficult because the number of M cells in vivo is small. Therefore, an in vitro human M cell model that can evaluate vaccines targeting M cells is required. If an in vitro human M cell model is constructed, we thought that we could contribute to the development of targeted oral vaccines and basic research on the function of human M cells. We constructed an in vitro human M cell model and evaluated the uptake of bacteria using cultured cells. M cell model was constructed by using the co-cultured of C2BBe1 and Raji cells, in which C2BBe1 was cultivated on the cell insert membrane and Raji cells in the well. We attempted to construct a new model by adding GST-RANKL, in which GST tag attached to RANKL(receptor activator of NF-κB ligand), a protein involved in differentiation into M cells. After co-culture, the number of fluorescent beads that was uptake by M cell (a functional evaluation), and the expression level of the M cell marker sialyl Lewis A antigen were compared with the co-culture model. In both evaluations, the new model showed improvement compared to the co-culture model.</p>

    DOI: 10.1254/jpssuppl.94.0_1-o-g2-4

    CiNii Articles

  • Draft Genome Sequences of Non-H 2 S-Producing Strains of Salmonella enterica Serovars Infantis, Enteritidis, Berta, and Kiambu in Japan. Reviewed

    Asakura H, Sakata J, Yamamoto S, Igimi S.

    Microbiol Resour Announc. (2020)   9 ( 30 )   2020.07

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

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