Papers - HIRAYAMA Hiroki
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Calving prediction with continuous measurement of subcutaneous tissue glucose concentration in pregnant cows Reviewed International journal
Wakatsuki, Takuji Nakamura, Tsukasa Ishii, Ayumi Konishi, Kanta Okubo, Michiko Souma, Kousaku Hirayama, Hiroki
Theriogenology 202 119 - 124 2023
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
To reduce losses of dams and calves due to unfortunate events, such as dystocia and freezing to death, identifying the onset of calving and providing necessary assistance are crucial. Prepartum increase in blood glucose concentration is a known indicator to detect labor in pregnant cows. However, some issues, including the need for frequent blood sampling and stress on cows, must be resolved before establishing a method for anticipating calving using changes in blood glucose concentrations. Herein, instead of measuring the blood glucose concentrations, subcutaneous tissue glucose concentration (tGLU) was measured in peripartum primiparous (n = 6) and multiparous (n = 8) cows at 15 min intervals using a wearable sensor. A transient increase in tGLU was observed in the peripartum period, with peak individual concentrations occurring between 2.8 h before and 3.5 h after calving. tGLU in primiparous cows was significantly higher than that in multiparous cows. To account for individual variations in basal tGLU, the maximum relative increase in the 3-h moving average of tGLU (Max MA) was used to predict calving. Cutoff points for Max MA were established by parity, with receiver operating characteristic analysis predicting calving within 24, 18, 12, and 6 h. Except for one multiparous cow that showed an increase in tGLU just before calving, all cows reached at least two cutoff points and calving was predicted successfully. The time interval between reaching the tGLU cutoff points that predicted calving within 12 h and actual calving was 12.3 ± 5.6 h. In conclusion, this study demonstrated the potential role of tGLU as a predictive indicator of calving in cows. Advancements in machine learning-based prediction algorithms and bovine-optimized sensors will help in increasing the accuracy of calving prediction using tGLU.
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Impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cows Reviewed International journal
INABA Ryo, KAWAHARA-MIKI Ryouka, SHINOZAWA Akihisa, YASUHARA Taichi, FUJII Takashi, KOYAMA Keisuke, MURATA-OKUBO Michiko, SOUMA Kousaku, HIRAYAMA Hiroki
Journal of Reproduction and Development 68 ( 1 ) 30 - 37 2022
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：The Society for Reproduction and Development
<p> Although hormonal induction of parturition in cattle results in the successful delivery of healthy calves, the risk of retained fetal membrane is significantly increased. In a previous study, a combination of the long-acting glucocorticoid, triamcinolone acetonide, with a high dose of betamethasone partially normalized the placentomal gene expression during parturition; however, the incidence of retained fetal membrane remained high. This study further explored placentomal dysfunction and aimed to elucidate the mechanism of retained fetal membrane in parturition-induced cows. In this study, transcriptome analysis revealed that enhanced glucocorticoid exposure normalized the expression of a substantial fraction of genes in the cotyledons. In contrast, a significant reduction in the multiple signaling pathway activities, including interferon signaling, was found in the caruncles during induced parturition. Real-time PCR showed that the expression of interferon-tau in the caruncles, but not interferon-alpha or interferon-gamma, was significantly lower in induced parturition than spontaneous parturition. Interferon-stimulated gene expression was also significantly decreased in the caruncles during induced parturition. These results indicate that interferon signaling could be important for immunological control in placentomes during parturition. Additionally, this suggests that interferon-tau might be a pivotal ligand for interferon receptors in the caruncles. This study revealed that peripheral blood leukocytes in prepartum cows transcribed interferon-tau. Macrophage infiltration in the placentome is known to participate in the detachment of the fetal membrane from the caruncle. Thus, this study raised the possibility that immune cells migrating into the caruncles at parturition may act as a source of ligands that activate interferon signaling.</p>
DOI： 10.1262/jrd.2021-094
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Expression of C-C motif chemokines and their receptors in bovine placentomes at spontaneous and induced parturition. Reviewed International journal
Hirayama, Hiroki Sakumoto, Ryosuke Koyama, Keisuke Yasuhara, Taichi Hasegawa, Taito Inaba, Ryo Fujii, Takashi Naito, Akira Moriyasu, Satoru Kageyama, Soichi
The Journal of reproduction and development 2020
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Mitochondrial DNA variations in Japanese farmed emu populations Reviewed International journal
KOSHIISHI Yuichi, UKITA Misuzu, MURATA-OKUBO Michiko, FUJISAWA Shin-ichiro, SHIMOI Gaku, HIRAYAMA Hiroki, KAMEYAMA Yuichi, SOUMA Kousaku, WADA Kenta
Journal of Veterinary Medical Science 82 ( 6 ) 731 - 734 2020
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
<p>The emu (<i>Dromaius novaehollandiae</i>) is a new poultry. In this study, we investigated the haplotype composition of mitochondrial DNA among emu populations farmed in Japan. We sequenced the D-loop region in 109 individuals, and detected four substitution sites and three haplotypes (Hap-a, -b, and -c). Hap-a was the most frequently observed haplotype in the Japanese populations. Although Hap-c was a rare haplotype in not only Japanese but also Australian populations, it was detected with high frequency in the Japanese farmed population. The AMOVA indicated that 9% of total variance was “among population”. The <i>F</i><sub>ST</sub> value was 0.087 and genetic differentiation was significant (<i>P</i><0.01). These results may contribute to conserving the genetic resources available for the Japanese emu industry.</p>
DOI： 10.1292/jvms.19-0693
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Development and characterization of ten novel microsatellite loci for the emu (Dromaius novaehollandiae) and genetic diversity of Japanese farm populations. Reviewed International journal
Koshiishi, Yuichi Murata-Okubo, Michiko Fujisawa, Shin-Ichiro Shimoi, Gaku Hirayama, Hiroki Kameyama, Yuichi Souma, Kousaku Wada, Kenta
Molecular biology reports 2020
Language：English Publishing type：Research paper (scientific journal)
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Development of 49 novel microsatellite markers from Next-generation sequencing data and a robust method for parentage tests in the emu (Dromaius novaehollandiae). Reviewed International journal
Koshiishi, Yuichi Uchiyama, Hironobu Murata-Okubo, Michiko Tanaka, Keisuke Kameyama, Yuichi Hirayama, Hiroki Wada, Kenta
Gene 2020
Language：English Publishing type：Research paper (scientific journal)
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Expression of C–C motif chemokines and their receptors in bovine placentomes at spontaneous and induced parturition Reviewed International journal
HIRAYAMA Hiroki, SAKUMOTO Ryosuke, KOYAMA Keisuke, YASUHARA Taichi, HASEGAWA Taito, INABA Ryo, FUJII Takashi, NAITO Akira, MORIYASU Satoru, KAGEYAMA Soichi
Journal of Reproduction and Development 66 ( 1 ) 49 - 55 2020
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：The Society for Reproduction and Development
<p> In bovine placentomes, the inflammatory response is considered important for the detachment of the fetal membrane from the caruncle after parturition. Glucocorticoids, a trigger of the onset of parturition, facilitate functional maturation of placentomes via prostaglandin (PG) and estrogen production in cattle. This study investigated how exogeneous glucocorticoids, which exert immunosuppressive effects, affect placental inflammation at parturition. Placentomes were collected immediately after spontaneous or induced parturition. Parturition was conventionally induced using PGF2α or dexamethasone or with a combination of triamcinolone acetonide and high-dose betamethasone (TABET treatment). Polymerase chain reaction (PCR) array analysis indicated that 9/13 C–C motif chemokine ligands (<i>CCLs</i>) were upregulated > two-fold in spontaneous parturition, with <i>CCL2</i> and <i>CCL8</i> being highly expressed. The expressions of <i>CCL2</i>, <i>CCL8,</i> C–C motif chemokine receptor 1 (<i>CCR1</i>), and <i>CCR5</i> in caruncles were significantly higher in spontaneous parturition than in induced parturition. Although the clinical dose of dexamethasone did not influence the expression of these <i>CCL</i>s and <i>CCR</i>s, TABET treatment increased <i>CCR1</i> expression. CCL8, CCR1, CCR2, and CCR5 were localized in the caruncular epithelial cells. CCR2 was also localized in the epithelial cells of the cotyledonary villi. This study is the first report to reveal the disruption in <i>CCL</i> and <i>CCR</i> expression in bovine placentomes at induced parturition. Enhanced glucocorticoid exposure for the induction of parturition may upregulate <i>CCR1</i> expression in placentomes, but the treatment does not adequately promote <i>CCL</i> expression. Additionally, immunohistochemistry suggested that the CCL–CCR system is involved in the functional regulation of maternal and fetal epithelial cells in placentomes at parturition.</p>
DOI： 10.1262/jrd.2019-113
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Potential of preimplantation genomic selection for carcass traits in Japanese Black cattle. Reviewed International journal
Fujii, Takashi Naito, Akira Hirayama, Hiroki Kashima, Masashi Yoshino, Hitomi Hanamure, Takeshi Domon, Yukio Hayakawa, Hiroyuki Watanabe, Toshio Moriyasu, Satoru Kageyama, Soichi
The Journal of reproduction and development 2019
Language：English Publishing type：Research paper (scientific journal)
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Enhanced glucocorticoid exposure facilitates the expression of genes involved in prostaglandin and estrogen syntheses in bovine placentomes at induced parturition. Reviewed International journal
Yasuhara, Taichi Koyama, Keisuke Sakumoto, Ryosuke Fujii, Takashi Naito, Akira Moriyasu, Satoru Kageyama, Soichi Hirayama, Hiroki
Theriogenology 2019
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Effects of genetic background on responses to superovulation in Japanese Black cattle. Reviewed International journal
Hirayama, Hiroki Naito, Akira Fujii, Takashi Sugimoto, Masahito Takedomi, Toshiro Moriyasu, Satoru Sakai, Hitomi Kageyama, Soichi
The Journal of veterinary medical science 2019
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Expression and localization of aquaporins 3 and 7 in bull spermatozoa and their relevance to sperm motility after cryopreservation. Reviewed International journal
Fujii, Takashi Hirayama, Hiroki Fukuda, Shigeo Kageyama, Soichi Naito, Akira Yoshino, Hitomi Moriyasu, Satoru Yamazaki, Takashi Sakamoto, Kozo Hayakawa, Hiroyuki Takahashi, Kenichi Takahashi, Yoshiyuki Sawai, Ken
The Journal of reproduction and development 2018
Language：English Publishing type：Research paper (scientific journal)
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Long-term changes in plasma anti-Müllerian hormone concentration and the relationship with superovulatory response in Japanese Black cattle. Reviewed International journal
Hirayama H, Naito A, Fukuda S, Fujii T, Asada M, Inaba Y, Takedomi T, Kawamata M, Moriyasu S, Kageyama S.
J Reprod Dev 63 95 - 100 2017
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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普及と研究が連携した受胎率向上技術の経済効果試算方法 Reviewed
山岸修一, 藤井貴志, 森安悟, 陰山聡一, 平山博樹, 片桐成二
農業普及研究HOKKAIDO 43 1 - 7 2017
Language：Japanese Publishing type：Research paper (scientific journal)
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Production of calves by the transfer of cryopreserved bovine elongating conceptuses and possible application for preimplantation genomic selection. Reviewed International journal
Fujii, Takashi Hirayama, Hiroki Naito, Akira Kashima, Masashi Sakai, Hitomi Fukuda, Shigeo Yoshino, Hitomi Moriyasu, Satoru Kageyama, Soichi Sugimoto, Yoshikazu Matsuyama, Shuichi Hayakawa, Hiroyuki Kimura, Koji
The Journal of reproduction and development 2017
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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The Necessity of OCT-4 and CDX2 for Early Development and Gene Expression Involved in Differentiation of Inner Cell Mass and Trophectoderm Lineages in Bovine Embryos. Reviewed International journal
Sakurai N, Takahashi K, Emura N, Fujii T, Hirayama H, Kageyama S, Hashizume T, Sawai K.
Cell Reprogram 18 309 - 318 2016
Language：English Publishing type：Research paper (scientific journal)
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Regulation of bovine oviductal NO synthesis by follicular steroids and prostaglandins. Reviewed International journal
Kobayashi Y, Yamamoto Y, Kageyama S, Hirayama H, Kimura K, Okuda K.
Reproduction 151 577 - 587 2016
Language：English Publishing type：Research paper (scientific journal)
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Localization of TGF-β and TGF-β receptor in bovine term placentome and expression differences between spontaneous and induced parturition Reviewed International journal
Hirayama H, Koyama K, Sawai K, Fujii T, Naito A, Fukuda S, Kageyama S
Placenta 36 ( 11 ) 1239 - 1245 2015.11
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Genetic diagnosis of band 3 deficiency using a quenching probe (QProbe)-PCR assay in bovine embryos Reviewed
Kageyama S, Hirayama H, Moriyasu S, Minamihashi A
Jpn J Vet Res 63 45 - 51 2015.02
Language：English Publishing type：Research paper (scientific journal)
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Enhancement of maternal recognition of pregnancy with parthenogenetic embryos in bovine embryo transfer. Reviewed International journal
Hirayama H, Moriyasu S, Kageyama S, Sawai K, Takahashi H, Geshi M, Fujii T, Koyama T, Koyama K, Miyamoto A, Matsui M, Minamihashi A.
Theriogenology 81 ( 8 ) 1108 - 1115 2014.03
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
子宮内でウシ胚が分泌し、母体の妊娠認識を誘導するインターフェロンτの補給源として人為的に卵子を活性化して作製した雌性単為発生胚が有効であることを明らかにした。細胞数が少ない低品質体内受精胚と単為発生胚を同時に受胚牛に移植することにより体内受精胚の受胎率が向上することを示した。
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Changes in the expression patterns of the genes involved in the segregation and function of inner cell mass and trophectoderm lineages during porcine preimplantation development. Reviewed International journal
Fujii T, Sakurai N, Osaki T, Iwagami G, Hirayama H, Minamihashi A, Hashizume T, Sawai K.
J Reprod Dev 59 ( 2 ) 151 - 158 2013.04
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
ブタ体内受精胚の細胞分化に関係する遺伝子の発現量を明らかにした。OCT4などを始めとする内部細胞塊と栄養膜細胞の分化を制御する遺伝子の発現量が、胚盤胞期から伸長期胚への発育に伴い変化することを示した。