Papers - SHIRASUNA Koumei
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Elevated S100A9 in preeclampsia induces soluble endoglin and IL-1b secretion and hypertension via the NLRP3 inflammasome. Reviewed
Ozeki A, Oogaki Y, Henmi Y, Karasawa T, Takahashi M, Takahashi H, Ohkuchi A, Shirasuna K
J Hypertens 40 84 - 93 2022
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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β-hydroxybutyrate suppresses NLRP3 inflammasome-mediated placental inflammation and lipopolysaccharide-induced fetal absorption. Reviewed
Hirata Y, Shimazaki S, Suzuki S, Henmi Y, Komiyama H, Kuwayama T, Iwata H, Karasawa T, Takahashi M, Takahashi H, Shirasuna K
J Reproductive Immunology 148 103433 2022
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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NLRP3 inflammasome is involved in testicular inflammation induced by lipopolysaccharide in mice. Reviewed
Sano M, Komiyama H, Shinoda R, Ozawa R, Watanabe H, Karasawa T, Takahashi M, Torii Y, Iwata H, Kuwayama T, Shirasuna K
American Journal of Reproductive Immunology 87 e13527 2022
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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IL1B triggers inflammatory cytokine production in bovine oviduct epithelial cells and induces neutrophil accumulation via CCL2. Reviewed
Nakamura Y, Aihara R, Iwata H, Kuwayama T, Shirasuna K
American Journal of Reproductive Immunology 85 ( 5 ) e13365 2021
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Metabolomic profiles of plasma and uterine luminal fluids from healthy and repeat breeder Holstein cows. Reviewed
Funeshima N, Miura R, Katoh T, Yaginuma H, Kitou T, Yoshimura I, Konda K, Hamano S, Shirasuna K
BMC Veterinary Research 17 ( 54 ) 2021
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Advanced maternal age induces fetal growth restriction with a decrease in placental inflammatory cytokines and immune cell accumulation in mice. Reviewed
Hirata Y, Katsukura Y, Henmi Y, Ozawa R, Shimazaki S, Kurosawa A, Karasawa T, Takahashi M, Ohkuchi A, Takahashi H, Kurosawa A, Torii Y, Takahashi H, Iwata H, Kuwayama T, Shirasuna K
J Reprod Dev 67 257 - 264 2021
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Uncaria tomentosa extract (AC-11) improves pregnancy hypertension together with suppression of sFlt-1 and sEng. Reviewed
Oogaki Y, Ozawa R, Seshima K, Shinoda R, Torii Y, Takahashi H, Iwata H, Kuwayama T, Shirasuna K
Pregnancy Hypertens 26 127 - 132 2021
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Role of the NLRP3 inflammasome in Preeclampsia Reviewed
Shirasuna K, Karasawa T, Takahashi M
Frontiers in Endocrinology 2020
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Palmitic adic activates NLRP3 inflammasome and induces placental inflammation during pregnancy in mice Reviewed
Sano M, Shimazaki S, Kaneko Y, Karasawa T, Takahashi M, Ohkuchi A, Takahashi H, Kurosawa A, Torii Y, Iwata H, Kuwayama T, Shirasuna K*
Journal of Reproduction and Development 2020
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Adverse reproductive effects of S100A9 on bovine sperm and early embryonic development in vitro Reviewed
Funeshima N, Tanikawa N, Yaginuma H, Watanabe H, Iwata H, Kuwayama T, Hamano S, Shirasuna K*
PLoS One 2020
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Preeclamptic patient-derived circulating cell-free DNA activates the production of inflammatory cytokines via toll-like receptor 9 signaling in the human placenta Reviewed
Ozeki A, Tani K, Takahashi H, Suzuki H, Nagayama S, Hirashima C, Takahashi H, Iwata H, Kuwayama T, Ohkuchi A, Shirasuna K*
Journal of Hypertension 37 2452 - 2460 2019
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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The transfer of parthenogenetic embryos following artificial insemination in cows can enhance pregnancy recognition via the secretion of interferon tau Reviewed
Funeshima N, Noguchi T, Onizawa Y, Yaginuma H, Miyamura M, Tsuchiya H, Iwata H, Kuwayama T, Hamano S, Shirasuna K*
Journal of Reproduction and Development 65 443 - 450 2019
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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S100A8, which increases with age, induces cellular senescence-like changes in bovine oviduct epithelial cells Reviewed
Nakamura Y, Iwata H, Kuwayama T, Shirasuna K*
American Journal of Reproductive Immunology 2019
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Olive leaf extract (OleaVita) suppresses inflammatory cytokine production and NLRP3 inflammasomes in human placenta Reviewed
Kaneko Y, Sano M, Seno K, Oogaki Y, Takahashi H, Ohkuchi A, Yokozawa M, Yamauchi K, Iwata H, Kuwayama T, Shirasuna K*
Neutrients 2019
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Improvement of fertility in repeat breeder dairy cattle by embryo transfer following artificial insemination: possibility of interferon tau replenishment effect Reviewed
Yaginuma H, Funeshima N, Tanikawa N, Miyamura M, Tsuchiya H, Noguchi T, Iwata H, Kuwayama T, Shirasuna K*, Hamano S*
Journal of Reproduction and Development 65 223 - 229 2019
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Age-related changes in the bovine corpus luteum function and progesterone secretion Reviewed
Hori K, Matsuyama S, Nakamura S, Iwata H, Kuwayama T, Miyamoto A, Shirasuna K*
Reproduction in Domestic Animals 54 23 - 30 2019
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Exogenous nanoparticles and endogenous crystalline molecules as danger signals for the NLRP3 inflammasomes. Reviewed
Shirasuna K, Karasawa T, Takahashi M
Journal of Cellular Physiology In press 2018.11
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Aggregation of human trophoblast cells into three-dimensional culture system enhances anti-inflammatory characteristics through cytoskeleton regulation. Reviewed
Seno K, Munakata Y, Sano M, Kawahara-Miki R, Takahashi H, Ohkuchi A, Iwata H, Kuwayama T, Shirasuna K
Int J Mol Sci 19 E2322 2018.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Oxygen concentration modulates cellular senescence and autophagy in human trophoblast cells. Reviewed
Seno K, Tanikawa N, Takahashi H, Ohkuchi A, Suzuki H, Matsubara S, Iwata H, Kuwayama T, Shirasuna K
Am J Reprod Immunol In press 2018
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Markedly higher sFlt-1/PlGF ratio in a woman with acute fatty liver of pregnancy compared with HELLP syndrome. Reviewed
Suzuki H, Nagayama S, Hirashima C, Takahashi K, Ogoyama M, Nagayama M, Shirasuna K, Matsubara S, Ohkuchi A
J Obstet Gynaecol Res In press 2018
Language：English Publishing type：Research paper (scientific journal)
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Short-term heat stress induces mitochondrial degradation and biogenesis and enhances mitochondrial quality in porcine oocytes. Reviewed
Itami N, Shirasuna K, Kuwayama T, Iwata H
J Therm Biol 74 256 - 263 2018
Language：English Publishing type：Research paper (scientific journal)
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Palmitic acid induces ceramide accumulation, mitochondrial protein hyper-acetylation and micochondrial dysfunction in porcine oocytes Reviewed
Itami N, Shirasuna K, Kuwayama T, Iwata H
Biol Reprod 98 644 - 653 2018
Language：English Publishing type：Research paper (scientific journal)
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Galectin-1 as a novel risk factor for both gestational hypertention and preeclampsia, specifically its expression at a low level in the second trimester and high level after onset. Reviewed
Hirashima C, Ohkuchi A, Nagayama S, Suzuki H, Takahashi K, Ogoyama M, Takahashi H, Shirasuna K, Matsubara S
Hypertens Res 41 45 - 52 2018
Language：English Publishing type：Research paper (scientific journal)
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Advanced glycation end products and lipopolysaccharide stimulate interleukin-6 secretion via RAGE/TLR4-NF-kB-ROS pathways and resveratrol attenuates these inflammatory responses in mouse macrophages. Reviewed
Ohtsu A, Shibutani Y, Seno K, Iwata H, Kuwayama T, Shirasuna K.
Experimental and Therapeutic Medicine 14 4364 - 4370 2017
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Palmitic acid stimulates interleukin-8 via the TLR4/NF-kB/ROS pathway and induces mitochondrial dysfunction in bovine oviduct epithelial cells. Reviewed
Ohtsu A, Tanaka H, Seno K, Iwata H, Kuwayama T, Shirasuna K.
Am J Reprod Immunol 2017
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Advanced glycation end products regulate interleukin-1β production in human placenta. Reviewed
Seno K, Sase S, Ozeki A, Takahashi H, Ohkuchi A, Suzuki H, Matsubara S, Iwata H, Kuwayama T, Shirasuna K
J Reprod Dev 63 401 - 408 2017
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Effect of aging on the female reproductive function. Invited Reviewed
Shirasuna K, Iwata H.
Contraception and Reproductive Medicine 2 ( 23 ) 2017
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Interferon tau regulates cytokine production and cellular function in human trophoblast cell line. Reviewed
Tanikawa N, Seno K, Kawahara-Miki R, Kimura K, Matsuyama S, Iwata H, Kuwayama T, Shirasuna K
J Interferon & Cytokine Research 37 456 - 466 2017
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Age-associated mRNA expression changes in bovine endometrial cells in vitro. Reviewed
Tanikawa N, Ohtsu A, Kawahara-Miki R, Kimura K, Matsuyama S, Iwata H, Kuwayama T, Shirasuna K
Reprod Biol Endocrinol 15 ( 63 ) 2017
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Maternal aging affects oocyte resilience to carbonyl cyanide-m-chlorophenylhydrazone-induced mitochondrial dysfunction in cows. Reviewed
Kansaku K, Takeo S, Itami N, Kin A, Shirasuna K, Kuwayama T, Iwata H
PLoS One 12 e0188099 2017
Language：English Publishing type：Research paper (scientific journal)
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Independent risk factors for a small placenta and a small-for-gestational-age infant at 35-41 weeks of gestation: An association with circulating angiogenesis-related factor levels at 19-31 weeks of gestation. Reviewed
Hirashima C, Ohkuchi A, Takahashi K, Suzuki H, Shirasuna K, Matsubara S
J Obestet Gynaecol Res 43 1285 - 1292 2017
Language：English Publishing type：Research paper (scientific journal)
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Promotion of glucose utilization by insulin enhances granulosa cell proliferation and developmental competence of porcine oocyte growth in vitro. Reviewed
Itami N, Munakata Y, Shirasuna K, Kuwayama T, Iwata H
Zygote 25 65 - 74 2017
Language：English Publishing type：Research paper (scientific journal)
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AGEs and HMGB1 increase inflammatory cytokine production from human placental cells, resulting in an enhancement of monocyte migration. Reviewed
Shirasuna K, Seno K, Ohtsu A, Shiratsuki S, Ohkuchi A, Suzuki H, Matsubara S, Nagayama S, Iwata H, Kuwayama T.
Am J Reprod Immunol 2016 ( 75 ) 557 - 568 2016
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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NLRP3 deficiency reduces macrophage interleukin-10 production and enhances the susceptibility to doxorubicin-induced cardiotoxicity. Reviewed
Kobayashi M, Usui F, Karasawa T, Kawashima A, Kimura H, Mizushina Y Shirasuna K, Mizukami H, Kasahara T, Hasebe N, Takahashi M.
Scientific Reports 6 2648 2016
Language：English Publishing type：Research paper (scientific journal)
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Age-dependent changes in inflammation and extracellular matrix in bovine oviduct epithelial cells during post-ovulatory phase. Reviewed
Tanaka H, Ohtsu A, Shiratsuki S, Kawahara-Miki R, Iwata H, Kuwayama T, Shirasuna K.
Mol Reprod Dev 2016 ( 83 ) 815 - 826 2016
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Palmitic acid induces interleukin-1β secretion via NLRP3 inflammasomes and inflammatory responses through ROS production in human placental cells. Reviewed
Shirasuna K, Takano H, Seno K, Ohtsu A, Karasawa T, Takahashi M, Ohkuchi A, Suzuki H, Matsubara S, Iwata H, Kuwayama T.
J Reprod Immunol 2016 ( 116 ) 104 - 112 2016
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Low oxygen level increases proliferation and metabolic changes in bovine granulosa cells. Reviewed
Shiratsuki S, Hara T, Munakata K, Shirasuna K, Kuwayama T, Iwata H
Mol Cell Endocrinol 437 75 - 85 2016
Language：English Publishing type：Research paper (scientific journal)
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Nanosilica-induced placental inflammation and pregnancy complications: Different roles of the inflammasome components NLRP3 and ASC Reviewed
Shirasuna K, Usui F, Karasawa T, Kimura H, Kawashima A, Mizukami H, Ohkuchi A, Nishimura S, Sagara J, Noda T, Ozawa K, Taniguchi S, Takahashi M.
Nanotoxicology 2015 ( 9 ) 554 - 567 2015.09
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Moderate hypoxia down-regulates interleukin-6 and TLR4 expression in human Sw.71 placental cells Reviewed
Shirasuna K, Shimamura N, Seno K, Ohtsu A, Shiratsuki S, Ohkuchi A, Suzuki H, Matsubara S, Nagayama S, Iwata H, Kuwayama T
Cell Physiol Biochem 2015 ( 36 ) 2149 - 2160 2015
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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NLRP3 deficiency improves angiotensin II-induced hypertension but not fetal growth restriction during pregnancy. Reviewed
Shirasuna K, Karasawa T, Usui F, Kobayashi M, Komada T, Kimura H, Kawashima A, Ohkuchi A, Taniguchi S, Takahashi M.
Endocrinology 2015 ( 156 ) 4281 - 4292 2015
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Possible role of IFNT on the bovine corpus luteum and neutrophils during the early pregnancy Reviewed
Shirasuna K, Matsumoto H, Matsuyama S, Kimura K, Bollwein H, Miyamoto A.
Reproduction 2015 ( 150 ) 217 - 225 2015
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Immunoproteasome subunit LPM7 deficiency improves obesity and metabolic disorders Reviewed
29) Kimura H, K Usui F, arasawa T, Kawashima A, Shirasuna K, Inoue Y, Komada T, Kobayashi M, Mizushina Y, Kasahara T, Suzuki K, Iwasaki Y, Yada T, Caturegli P, Takahashi M
Sci Rep 5 15883 2015
Language：English Publishing type：Research paper (scientific journal)
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Interferon-tau attenuates uptake of nanoparticles and secretion of interleukin-1b in macrophages Reviewed
Hara K, Shirasuna K, Usui F, Karasawa T, Mizushina Y, Kimura H, Kawashima A, Ohkuchi A, Matsuyama S, Kimura K, Takahashi M.
PLOS ONE 2014 ( 9 ) e113974 2014.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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The effect of single nucleotide polymorphisms in the tumor necrosis factor-a gene on reproductive performance and immune function in dairy cattle. Reviewed
Kawasaki Y, Aoki Y, Magata F, Miyamoto A, Kawashima C, Hojo T, Okuda K, Shirasuna K, Shimizu T.
Journal of Reproduction and Development 2014 ( 60 ) 173 - 178 2014
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Downregulation of lymphatic vessel formation factors in PGF2α -induced luteolysis in the cow.
Nitta A, Shirasuna K*, Nibuno S, Bollwein H, Shimizu T, Miyamoto A
Journal of Reproduction and Development 59(3)IF：1.755 2013.01
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
PGF誘導性黄体退行中の黄体内ではリンパ管新生が急激に抑制されること、その抑制にはTNFが関与していることを示しました。
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Emerging roles of immune cells in luteal angiogenesis.
Shirasuna K,Shimizu T,Motozumi M, Miyamoto A
Reproduction Fertility and Development 25(2)IF：2.583 2013.01
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
ウシ黄体血管新生における免疫細胞の新しい役割の概念をまとめました。
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Treatment of estradiol during early postpartum enhances the recovery of ovarian activity in dairy cows.
Yamaguchi Y, Shimoda T, Shirasuna K, Kawashima C, Miyamoto A
Journal of the Japan Veterinary Medical Association 64(11) 2012.11
Language：English Publishing type：Research paper (scientific journal)
分娩後の無発情牛にエストロジェン製剤を筋肉内投与することで、投与後の発情回帰および排卵を誘導することが可能になりました。
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Effects of lipopolysaccharide (LPS) and peptidoglycan (PGN) on estradiol production in bovine granulosa cells from small and large follicles.
Shimizu T, Miyauchi K, Shirasuna K, Bollwein H, Magata F, Murayama C, Miyamoto A
Toxicology in Vitro 26(7)IF：2.650 2012.10
Language：English Publishing type：Research paper (scientific journal)
ウシ顆粒層細胞を用い、リポポリサッカライドやペプチドグリカンなどの細菌由来成分がステロイド産生に及ぼす影響を検証しました。
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Escherichia. coli lipopolysaccharide administration transiently suppresses luteal structure and function in dioestrous cows.
Herzog K, Struve K, Kastelic JP, Piechotta M, Ulbrich SE, Pfarrer C, Shirasuna K, Shimizu T, Miyamoto A, Bollwein H
Reproduction 144(4)IF：3.555 2012.10
Language：English Publishing type：Research paper (scientific journal)
発情周期中のウシに大腸菌由来リポポリサッカライドを投与すると、黄体内で一過性にプロジェステロン産生の減少が起き、アポトーシスが誘導されることを示しました。
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Expression of prostaglandin F2α (PGF2α) receptor and its isoforms in the bovine corpus luteum during the estrous cycle and PGF2α -induced luteolysis.
Shirasuna K, Akabane Y, Beindorff N, Nagai K, Sasaki M, Shimizu T, Bollwein H, Meidan R, Miyamoto A
Domestic Animal Endocrinology 43(3)IF：2.377 2012.10
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
発情周期中および黄体退行中において、黄体内でPGFの受容体アイソフォームが存在することを示しました。また、siRNAを用いた実験で、PGF受容体アイソフォームごとにその機能が異なることを示しました。
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Critical role of caspase-1 in vascular inflammation and development of atherosclerosis in Western diet-fed apolipoprotein E-deficient mice.
Usui F, Shirasuna K, Kimura H, Tatsumi K, Kawashima A, Karasawa T, Hida S, Sagara J, Taniguchi S, Takahashi M
Biochemical and Biophysical Research Communications 425(2)IF：2.406 2012.08
Language：English Publishing type：Research paper (scientific journal)
動脈硬化が誘導される肥満マウスモデルを用い、炎症性サイトカインであるIL-1b産生に必須であるCaspase-1が動脈硬化の発生および血管炎症の誘導に重要であることを示しました。
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Vascular and immune regulation of corpus luteum development, maintenance, and regression in the cow.
Shirasuna K, Nitta A, Sineenard J, Shimizu T, Bollwein H, Miyamoto A
Domestic Animal Endocrinology 43(2)IF：2.377 2012.08
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
ウシ黄体機能調節における血管調節因子および免疫細胞の役割についてまとめました。
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Possible action of vasohibin-1 as an inhibitor in the regulation of vascularization of the bovine corpus luteum.
Shirasuna K*, Kobayashi A, Nitta A, Nibuno S, Sasahara K, Shimizu T, Bollwein H, Miyamoto A
Reproduction 143(4)IF：3.555 2012.04
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
黄体形成に必須な血管新生において、適切な血管新生機構を制御するためのVasohibin-1が黄体内に存在することを示しました。またVasohibin-1は黄体血管新生を調節するだけではなく、リンパ管新生も制御することを見出しました。
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Gene expression in the persistent corpus luteum of postpartum dairy cows: distinct profiles from the corpora lutea of the estrous cycle and pregnancy.
Magata F, Shirasuna K, Struve K, Herzog K, Shimizu T, Bollwein H, Miyamoto A
Journal of Reproduction and Development 58(4)IF：1.755 2012.04
Language：English Publishing type：Research paper (scientific journal)
黄体退行が起きず、遺残してしまった黄体の機能性変化を検証しました。遺残黄体は通常黄体および妊娠黄体と比較して免疫応答性因子やサイトカインの発現が高く、黄体内の免疫機能異常が関与している可能性を提示しました。
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Upregulation of interferon-stimulated genes and interleukin-10 in peripheral blood immune cells during early pregnancy in dairy cows.
Shirasuna K, Matsumoto H, Kobayashi E, Nitta A, Haneda S, Matsui M, Kawashima C, Kida K, Shimizu T, Miyamoto A
Journal of Reproduction and Development 58(1)IF：1.755 2012.03
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
妊娠牛の末梢血免疫細胞では妊娠初期からインターフェロン刺激因子やインターロイキン10の遺伝子発現が増加することから、着床前の胚が母体へ妊娠シグナルを発信している可能性を提案しました。
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Effects of interleukin-8 on estradiol and progesterone production by bovine granulosa cells from large follicles and progesterone production by luteinizing granulosa cells in culture.
Shimizu T, Kaji A, Murayama C, Magata F, Shirasuna K,Wakamiya K, Okuda K, Miyamoto A
Cytokine 57(1)IF：2.518 2012.01
Language：English Publishing type：Research paper (scientific journal)
ウシ大卵胞由来の顆粒層細胞において、IL-8がステロイド産生機能に及ぼす影響をin vitroで検証しました。
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Rapid accumulation of polymorphonuclear neutrophils in the corpus luteum during prostaglandin F2α -induced luteolysis in the cow.
Shirasuna K*,Jiemtaweeboon S, Raddatz S, Nitta A,Schuberth HJ, Bollwein H, Shimizu T, Miyamoto A
PLoS ONE 7(1)IF：3.730 2012.01
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
黄体退行中ではPGFが好中球を5分で動員させる現象をin vivoで見出し、これはPGFが黄体血管内皮細胞の白血球接着分子のトランスロケーションを誘導し、好中球が黄体組織に迅速に入り込む現象であることを示しました。
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Possible involvement of IFNT in lymphangiogenesis in the corpus luteum during the maternal recognition period in the cow.
Nitta A, Shirasuna K*,Haneda S, Matsui M, Shimizu T, Matsuyama S, Kimura K, Schuberth HJ, Miyamoto A
Reproduction 142(6)IF：3.555 2011.12
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
黄体内にリンパ管が存在することを、遺伝子および免疫組織化学手法を用いて初めて示しました。黄体内リンパ管は黄体が妊娠黄体に移行する過程においてリンパ管の再構築が起きることを発見しました。3次元リンパ管内皮細胞培養モデルを構築し、胚から分泌されるインターフェロンτがリンパ管新生を直接刺激することを示し、妊娠成立のために黄体内の免疫応答機構が特異的に変化する新しい可能性を提示しました。
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Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow.
Jiemtaweeboon S, Shirasuna K*, Nitta A, Kobayashi A, Schuberth HJ, Shimizu T, Miyamoto A
Reproductive Biology and Endocrinology 9(79)IF：2.144 2011.06
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
排卵直後の黄体内には、免疫細胞の一種である好中球が集積し、好中球の走化性物質であるインターロイキン8（IL8）の遺伝子と産生レベルが高いことが分かりました。形成期黄体はIL8を分泌することで好中球を時期特異的に集積させることを証明し、好中球およびIL8が黄体由来血管内皮細胞の脈管形成を刺激することを示しました。
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Relationships between the first ovulation postpartum and polymorphism in genes relating to function of immunity, metabolism and reproduction in high-producing dairy cows.
Shirasuna K, Kawashima C, Murayama C, Aoki Y, Masuda Y, Kida K, Matsui M, Shimizu T, Miyamoto A
Journal of Reproduction and Development 57(1)IF：1.755 2011.02
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
乳牛の分娩後早期の初回排卵の有無と、様々な因子における遺伝的多型との関連性を検証しました。炎症性サイトカインであるTNFの遺伝的多型が初回排卵の有無と関連することを見出し、その後の繁殖性や乳量などの表現型にも影響を及ぼすことを示しました。
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Regulation of corpus luteum development and maintenance: specific roles of angiogenesis and action of prostaglandin F2α.
Miyamoto A, Shirasuna K, Shimizu T, Bollwein H, Schams D.
Journal of reproduction and fertility Supplement 67IF：3.555 2010.09
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
ウシ黄体形成と妊娠黄体移行における血管新生因子とPGF作用についてまとめました。
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Prostaglandin F2α differentially affects mRNA expression relating to angiogenesis, vasoactivation and prostaglandins in the early and mid corpus luteum in the cow.
Shirasuna K, Sasahara K, Matsui M, Shimizu T, Miyamoto
Journal of Reproduction and Development 56(4)IF：1.755 2010.08
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
黄体退行因子PGFは、黄体期中期では黄体退行を誘導することが可能ですが、黄体期初期では不可能です。この要因を検証し、PGFの作用が黄体期時期依存的に異なること、特に黄体期中期ではPGFは血管新生因子を抑制しますが、黄体期初期では逆にそれらを刺激するため、黄体退行を引き起こすことが不可能である可能性を示しました。
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Vascular changes in the corpus luteum during early pregnancy in the cow.
Beindorff N, Nagai K, Shirasuna K, Herzog K, Hoeffmann K, Sasaki M, Bollwein H, Miyamoto A
Journal of Reproduction and Development 56(2)IF：1.755 2010.04
Language：English Publishing type：Research paper (scientific journal)
ウシ妊娠初期黄体における血管構造の変化を検証しました。妊娠黄体移行期から黄体の血管構造が変化し、余分な毛細血管が減少する一方で大型の収縮性血管が増加することで、安定した黄体構造をとることが分かりました。
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Prostaglandin F2α represses IGF-I-stimulated IRS1/phosphatidylinositol-3-kinase/AKT signaling in the corpus luteum: role of ERK and P70 ribosomal S6 kinase.
Arvisais E, Hou X, Wyatt T, Shirasuna K, Bollwein H, Miyamoto A, Hansen TR, Rueda BR, DavisJS
Molecular Endocrinology 24(3)IF：4.746 2010.03
Language：English Publishing type：Research paper (scientific journal)
黄体退行を誘導するPGFの黄体内でのシグナルカスケードを検証しました。PGFは黄体細胞におけるIGF-1の下流シグナルを抑制し、黄体退行を誘導する可能性を提示しました。
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Nitric oxide and luteal blood flow in the luteolytic cascade in the cow.
Shirasuna K
Journal of Reproduction and Development 56(1)IF：1.755 2010.02
Language：English Publishing type：Research paper (scientific journal)
ウシ黄体退行直後に起きる黄体血流域増加と一酸化窒素についてまとめました。
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Distribution of arteriolovenous vessels, capillaries and eNOS expression in the bovine corpus luteum during the estrous cycle: a possible implication of different sensitivity by the luteal phase to PGF2α in the increase of luteal blood flow.
Shirasuna K, Asahi T, Sasaki M, Shimizu T, Miyamoto A
Journal of Reproduction and Development 56(1)IF：1.755 2010.02
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
黄体機能を調節する大型血管や毛細血管と内皮型一酸化窒素合成酵素の局在および発現変動を検証しました。黄体内ではこれらが不均一に局在し、この不均一性が退行時の黄体血流域増加現象に関連する可能性を示しました。
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Local regulation of the corpus luteum development and regression in the cow: Impact of angiogenic and vasoactive factors.
Miyamoto A, Shirasuna K, Sasahara K
Domestic Animal Endocrinology 37(3)IF：2.377 2009.10
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
ウシ黄体形成および黄体退行における血管新生因子や血管作動性因子の役割についてまとめました。
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Luteolysis in the cow: a novel concept of vasoactive molecules.
Miyamoto A, Shirasuna K
Animal Reproduction Science 6(1)IF：1.897 2009.01
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
ウシ黄体退行において血管拡張・収縮因子が黄体血流を調節する概念についてまとめました。