Details of a Researcher - MATSUSHIMA Yoshitaka

Papers - MATSUSHIMA Yoshitaka

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Application of the SpCas9 inhibitor BRD0539 for CRISPR/Cas9-based genetic tools in Lacticaseibacillus paracasei Reviewed

TAMAKI Mizuna, CHIKU Tsutomu, SUZUKI Shunya, MISAKI Aya, FUNAKUBO Asuka, MATSUSHIMA Yoshitaka, YOKOTA Kenji, IGIMI Shizunobu, KAJIKAWA Akinobu

Bioscience of Microbiota, Food and Health 44 ( 1 ) 70 - 79 2025

Language：English Publishing type：Research paper (scientific journal) Publisher：BMFH Press

Although the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas system has been extensively developed since its discovery for eukaryotic and prokaryotic genome editing and other genetic manipulations, there are still areas warranting improvement, especially regarding bacteria. In this study, BRD0539, a small-molecule inhibitor of Streptococcus pyogenes Cas9 (SpCas9), was used to suppress the activity of the nuclease during genetic modification of Lacticaseibacillus paracasei, as well as to regulate CRISPR interference (CRISPRi). First, we developed and validated a CRISPR-SpCas9 system targeting the sirA gene of L. paracasei. Then BRD0539 was used for CRISPR-dependent DNA cleavage in vivo. Our results suggested that the inhibitor worked partially in both Escherichia coli and L. paracasei. Next, we designed a CRISPRi system in a L. paracasei strain by inserting an inactive SpCas9 gene into the chromosome and introducing a plasmid encoding for a single guide RNA (sgRNA) targeting the sirA gene. Expression of sirA was successfully inhibited in the recombinant strains, and CRISPRi was abolished in an inhibitor-dependent manner. Our findings may help expand the CRISPR toolbox for research on lactic acid bacteria and other microbes.

DOI： 10.12938/bmfh.2024-009

Gram-scale approach for β-costic acid via allylic oxidation of β-selinene Reviewed International journal

Yoshitaka Matsushima, Kosuke Iwata

Bioscience, Biotechnology, and Biochemistry 88 ( 7 ) 742 - 746 2024.05

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： https://doi.org/10.1093/bbb/zbae052

Gram-scale synthesis of cytoxazone via dihydrooxazine formation through SiO2-promoted intramolecular substitution of a trichloroacetimidate Reviewed International journal

Yoshitaka Matsushima, Kota Otsuka, Moe Ishikawa, Shu Akita

Tetrahedron Letters 131 154586 2023.10

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： doi.org/10.1016/j.tetlet.2023.154786

Simple synthesis of violaceoid A, a cytotoxic hydroquinone Reviewed International journal

Yoshitaka Matsushima, Haruka Yoshioka, Naoya Sunada

Tetrahedron Letters 122 154522 2023.04

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.tetlet.2023.154522

Concise synthesis of amino acid component of amicoumacins via dihydrooxazine formation through intramolecular conjugate addition Reviewed International journal

Yoshitaka Matsushima, Yukako Ogawa, Katsuya Nishi, Kyosuke Nakata

Bioscience, Biotechnology, and Biochemistry 87 ( 2 ) 131 - 137 2023.02

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： doi.org/10.1093/bbb/zbac182

Enantioselective total syntheses of (S)-phosphonothrixin and unexpected cyclic derivative (S)-cyclic phosphonothrixin via enzymatic resolution Reviewed International journal

Koki Nakamura, Yoshitaka Matsushima

Bioscience, Biotechnology, and Biochemistry 87 ( 2 ) 138 - 147 2023.02

Authorship：Last author Language：English Publishing type：Research paper (scientific journal)

DOI： doi.org/10.1093/bbb/zbac188

Total synthesis of the methyl glycoside of bradyrhizose via intramolecular pinacol coupling Reviewed International journal

Yoshitaka Matsushima, Yutaro Ito, Hiroyuki Konno

Tetrahedron Letters 106 154060 2022.08

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.tetlet.2022.154060

Isolation of phenolic acids and tannin acids from Mangifera indica L. kernels as inhibitors of lipid accumulation in 3T3-L1 cells. Reviewed International journal

Takahiro Fujimaki, Chikako Sato, Rei Yamamoto, Sayo Watanabe, Hikaru Fujita, Hidehiko Kikuno, Masayuki Sue, Yoshitaka Matsushima

Bioscience, Biotechnology and Biochemistry 86 ( 5 ) 665 - 671 2022.04

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/bbb/zbac030

Efficient synthesis of zealexin B1, a maize sesquiterpenoid phytoalexin, via Suzuki-Miyaura coupling Reviewed International coauthorship International journal

Yoshitaka Matsushima, Kohei Ishii, Alisa Huffaker, Eric A. Schmelz

Tetrahedron Letters 91 153641 2022.01

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.tetlet.2022.153641

Concise and scalable synthesis of (±)-phosphonothrixin Reviewed International journal

Yoshitaka Matsushima, Koki Nakamura

Results in Chemistry 3 100251 2021.11

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.rechem.2021.100251

新スタンダード栄養・食物シリーズ17「有機化学の基礎」

松島芳隆

化学と生物 59 ( 10 ) 531 - 531 2021.10

Language：Japanese Publisher：公益社団法人日本農芸化学会

DOI： 10.1271/kagakutoseibutsu.59.531

強烈なインパクト

松島芳隆

化学と生物 59 ( 8 ) 363 - 363 2021.08

Language：Japanese Publisher：公益社団法人日本農芸化学会

DOI： 10.1271/kagakutoseibutsu.59.363

Geospatial Assessments of DNA Adducts in the Human Stomach: A Model of Field Cancerization Reviewed International journal

Yuji Iwashita, Ippei Ohnishi, Yuto Matsushita, Shunsuke Ohtsuka, Takashi Yamashita, Keisuke Inaba, Atsuko Fukazawa, Hideto Ochiai, Keigo Matsumoto, Nobuhito Kurono, Yoshitaka Matsushima, Hiroki Mori, Shioto Suzuki, Shohachi Suzuki, Fumihiko Tanioka, Haruhiko Sugimura

Cancers 13 3728 2021.07

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.3390/cancers13153728

GABA_A Receptors and Maternally Derived Taurine Regulate the Temporal Specification of Progenitors of Excitatory Glutamatergic Neurons in the Mouse Developing Cortex Reviewed International journal

Shiro Tochitani, Tomonori Furukawa, Ryo Bando, Shigeaki Kondo, Takashi Ito, Yoshitaka Matsushima, Toshio Kojima, Hideo Matsuzaki, Atsuo Fukuda

Cerebral Cortex 31 4554 - 4574 2021.05

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/cercor/bhab106

Concise synthesis of the Taxol side chain and demethoxy-4-epi-cytoxazone via oxazoline formation through intramolecular benzylic substitution of a bis-trichloroacetimidate Reviewed International journal

Yoshitaka Matsushima, Mina Orita

Tetrahedron Letters 73 153095 2021.05

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.tetlet.2021.153095

Mass spectrometric profiling of DNA adducts in the human stomach associated with damage from environmental factors Reviewed International journal

Ippei Ohnishi, Yuji Iwashita, Yuto Matsushita, Shunsuke Ohtsuka, Takashi Yamashita, Keisuke Inaba, Atsuko Fukazawa, Hideto Ochiai, Keigo Matsumoto, Nobuhito Kurono, Yoshitaka Matsushima, Hiroki Mori, Shioto Suzuki, Shohachi Suzuki, Fumihiko Tanioka and Haruhiko Sugimura

Genes and Environment 43 2021.04

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1186/s41021-021-00186-2

DNA Adductome Analysis Identifies N‐Nitrosopiperidine Involved in the Etiology of Esophageal Cancer in Cixian, China Reviewed International journal

Yukari Totsuka, Yingsong Lin, Yutong He, Kousuke Ishino, Haruna Sato, Mamoru Kato, Momoko Nagai, Asmaa Elzawahry, Yasushi Totoki, Hiromi Nakamura, Fumie Hosoda, Tatsuhiro Shibata, Tomonari Matsuda, Yoshitaka Matsushima, Guohui Song, Fanshu Meng, Dongfang Li, Junfeng Liu, Youlin Qiao, Wenqiang Wei, Manami Inoue, Shogo Kikuchi, Hitoshi Nakagama, and Baoen Shan

Chem. Res. Toxicol. 32 1515 - 1527 2019.06

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1021/acs.chemrestox.9b00017

Identification of the novel hcbB operon catalyzing the dechlorination of pentachlorophenol in the Gram-positive bacterium Nocardioides sp. strain PD653 Reviewed International journal

Koji Ito, Kazuhiro Takagi, Yoshitaka Matsushima, Akio Iwasaki, Naoto Tanaka, Yu Kanesaki, Fabrice Martin-Laurent, Shizunobu Igimi

J. Pestic. Sci. 43 ( 2 ) 124 - 131 2018.04

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1584/jpestics.D17-089

Short synthesis of epi-cytoxazone via oxazoline formation through intramolecular benzylic substitution of a bis-trichloroacetimidate Reviewed International journal

Yoshitaka Matsushima, Moe Ishikawa, Rina Shibasaki, Yuki Nojima

Tetrahedron Letters 59 231 - 233 2018.01

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.tetlet.2017.11.057

A versatile route to 2,4,6-trideoxy-4-aminohexoses: Stereoselective syntheses of D-vicenisamine and its epimers via iodocyclization of carbamate Reviewed International journal

Yoshitaka Matsushima, Jun Kino

Tetrahedron 73 6831 - 6839 2017.10

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.tet.2017.10.009

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Papers - MATSUSHIMA Yoshitaka