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Degree 【 display / non-display 】
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Doctor of Agriculture ( 2001.03 Kyoto University )
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Agriculture Department of Agriculture Professor
2025.03
External Career 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Agriculture Department of Agriculture Professor
2025.04
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National Agriculture and Food Research Organization
2021.04 - 2025.03
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University of Tsukuba Graduate School of Life and Environmental Sciences
2019.04 - 2025.03
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National Agriculture and Food Research Organization
2016.04 - 2021.03
Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Plant genetics and breeding
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Environmental Science/Agriculture Science / Horticulture
Research Interests 【 display / non-display 】
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花き育種
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日持ち性
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病害抵抗性
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ダリア
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カーネーション
Papers 【 display / non-display 】
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Crossing and Selection of Dahlia (Dahlia Cav.) Lines with a Lower Degree of Petal Abscission Reviewed International journal
Takuo Fujimoto, Takashi Onozaki
The Horticulture Journal 94 ( 1 ) 15 - 23 2025.01
Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society for Horticultural Science
Dahlia (Dahlia Cav.) is an important ornamental plant used as cut flowers. Recently, we bred the “Eternity series” cultivars with long vase life to overcome the difficulty of long-distance transport due to their short vase life. However, dahlias, including the Eternity series, are ethylene-sensitive, inducing petal abscission within a short period. Therefore, cultivars with long vase life and low degree of abscission were crossed to examine the possibility of producing lines with both traits. The flower life of offspring after treatment with 10 μL·L−1 ethylene (time to ethylene response) was investigated in open-field cultivation during the summer–autumn of 2021. The degree of abscission was classified into three types according to the time to ethylene response and the occurrence of abscission: abscission within four days (high), abscission after four days (mid), and the absence of abscission during senescence (low). We selected 17 of the 105 lines with mid or low degrees of abscission. The degree of abscission of the selected lines was re-evaluated during greenhouse cultivation in the winter–spring of 2021–2022. Three lines (F182-10, F181-14, and F182-17) that showed no petal abscission in the winter–spring were selected. The drawing resistance force of the petals from lines F182-10 and F182-17 was maintained at 1.0 N even during the senescence stage (six days after harvest), which was related to their low degree of abscission. The degree of abscission of lines F182-10, F181-14, and F182-17 was stable during the re-evaluation in summer–autumn of 2023, and their times to ethylene response were 4.8, 5.8, and 4.6 days, respectively. Moreover, the vase lives of lines F182-10 and F182-17 were 5.8 and 5.6 days, respectively, while that of ‘Port Light Pair Beauty’ was 4.4 days. These results suggested that degree of abscission was inherited from ‘Port Light Pair Beauty’, and the vase life was improved after crossing with a cultivar with a long vase life. This is the first report about breeding dahlia with a low degree of abscission, and it indicates the possibility of producing lines with both long vase life and a low degree of abscission.
DOI： 10.2503/hortj.qh-149
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ONOZAKI Takashi, FUJIMOTO Takuo, AZUMA Mirai
Journal of the NARO Research and Development 19 ( 1 ) 43 - 62 2024.11
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Research paper (bulletin of university, research institution) Publisher：NARO
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Development of new dahlia cultivars ‘Eternity Peach’ and ‘Eternity Shine’ with long vase lives International journal
T. Onozaki, T. Fujimoto, M. Azuma
Acta Horticulturae 1404 777 - 786 2024.09
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (international conference proceedings) Publisher：International Society for Horticultural Science (ISHS)
Improvement of flower vase life is an important breeding target in determining quality and the ability to satisfy consumer preferences. As the vase life of cut dahlia (Dahlia variabilis) flowers is very short, we initiated a research breeding program in 2014 to improve it. We chose 22 commercial cultivars as initial breeding materials, and we repeatedly crossed and selected promising offspring with long vase lives for five generations, from 2014 to 2021. Third-generation line 828-18, which has beautiful gradation flower color with a pink and white center, and line 937-C58 (‘Namahage Cute’ × second-generation line 628-48) with deep pink flowers were judged in 2022 to be suitable as new cultivars. We registered them and released them as ‘Eternity Peach’ and ‘Eternity Shine’ in 2023. These two new cultivars have genetically determined long vase lives of 8.0 to 10.7 days (1.4× to 2.0× that of ‘Kamakura’, a leading white dahlia cultivar in Japan) in distilled water, 9.5 to 11.4 days (1.4× to 1.8× ‘Kamakura’) in GLA solution (10 g L-1 glucose, 0.5 mL L-1 CMIT/MIT (isothiazolinone derivatives), and 50 mg L-1 aluminum sulfate), and 10.1 to 11.6 days (1.4× to 1.6× ‘Kamakura’) in GLA solution + 6-benzylaminopurine spray, under 23°C, 12-h photoperiod, and 70% RH. Cut flowers of ‘Eternity Shine’ grown under high temperatures in July-August and at 28°C with GLA treatment also had a long vase life. Moreover, cut flowers of ‘Eternity Shine’ had high transportability. After long-distance transport, ‘Eternity Shine’ kept its ornamental value over 10 days.
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Classification of ethylene sensitivity in cut flowers of dahlia and selection of ‘Eternity Shine’ with low ethylene sensitivity International journal
T. Fujimoto, T. Onozaki
Acta Horticulturae 1404 163 - 168 2024.09
Authorship：Last author Language：English Publishing type：Research paper (international conference proceedings) Publisher：International Society for Horticultural Science (ISHS)
Dahlia (Dahlia variabilis) is used for cut flowers, which has become popular in recent years. In 2020, we bred three cultivars with a genetically determined long vase life, ‘Eternity Torch’, ‘Eternity Romance’ and ‘Eternity Rouge’ (called the “Eternity series”). On the other hand, the value of cut flowers is lost in short period when petal abscission is induced by ethylene during storage and transportation, which is observed even in the Eternity series. In the present study, we investigated ethylene sensitivity of the dahlia cut flowers using commercial cultivars and lines with long vase life bred in our research group, to classify ethylene sensitivity. The ethylene sensitivity was investigated by response to 10 μL L-1 exogenous ethylene treatment, as the number of days from harvest until one-third of all petals showed wilting, or abscission of ten or more petals. As a result, there were large differences in ethylene sensitivity among cultivars and lines. The ethylene sensitivity of dahlia was classified into three types according to their vase life and occurrence of abscission: abscission within 4 days (High), abscission after 4 days (Middle), without abscission during senescence (Low). Totally 31 cultivars and 55 lines were used for the investigation, and classified 56, 27 and 3 were high, middle and low sensitivities, respectively. Furthermore, investigations were carried out in winter-spring and summer-autumn seasons, and it was found that there was seasonal variation in the ethylene sensitivity of dahlia cut flowers. Thus, lines with low ethylene sensitivity were selected, and ‘Eternity Shine’ was obtained which vase life was 10.0 days with 10 μL L-1 ethylene treatment in 2020 summer-autumn season. These cultivars and lines will be used in the future to clarify the genetic mechanism of ethylene response in dahlias.
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Takashi Onozaki, Takuo Fujimoto, Mirai Azuma
Journal of the NARO Research and Development 17 53 - 69 2024.03
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Research paper (bulletin of university, research institution) Publisher：NARO
Books and Other Publications 【 display / non-display 】
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Sho Ohno, Takashi Onozaki（ Role： Joint author）
Springer NAture 2025.03 （ ISBN:9783031778995 ）
Responsible for pages：313-341 Language：English Book type：Scholarly book
Dahlia (Dahlia variabilis, or D. pinnata) is one of the most popular garden and cut flowers around the world owing to its great variety of flower colors, sizes and shapes. Dahlia is an auto-allo-octoploid plant with 2n = 8x = 64, which enables enormous variation in many gene combinations, and more than 50,000 cultivars have been bred in the last century. This chapter summarizes recent research advances, with a focus on flower color and vase life. The pigments that confer flower color in dahlia are flavonoids: anthocyanins, flavones and 6ʹ-deoxychalcones. Anthocyanins and flavones are common pigments found in many plant species, while 6ʹ-deoxychalcones are found in a limited number of species. The amount and combination of pigments determine flower color. The first section describes the underlying molecular mechanisms of flower color. Findings from the recent publications about pigment biosynthetic genes, variegation and bicolor pattern formation are summarized, and at last, current schematic model for flower color breeding in dahlia is proposed. The second section summarizes breeding programs targeting vase life elongation and the development of the long-vase-life cultivars ‘Eternity Torch’, ‘Eternity Romance’ and ‘Eternity Rouge’. Dahlias are known as short-vase-life cut flowers, so extending their vase life increases their value. The process of development of new cultivars by cross-breeding is described. To improve the vase life of dahlia flowers, cross-bred selections were conducted for five generations and promising offsprings with long vase life were selected, from 2014 to 2021 using 22 commercial dahlia cultivars with large differences in vase life as initial breeding materials.
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ダリア 本邦初 良日持ち性品種エターニティシリーズの開発 最新農業技術 花卉 Vol.15, P.143-155
小野崎 隆
農文協，東京 2023.03 （ ISBN:9784540220593 ）
Responsible for pages：143-155 Language：Japanese Book type：Scholarly book
良日持ち性ダリア品種エターニティシリーズ3品種の開発について、育種の着眼点と育種法、育成経過、特性、全国普及に向けた現況、今後の普及戦略などを解説した。
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Takashi Onozaki, Masafumi Yagi（ Role： Edit）
Springer 2020.12 （ ISBN:9789811582608 ）
Total pages：183 Language：English Book type：Scholarly book
This book summarizes recent advances in carnation genome research for large-scale transcriptome analysis, the draft genome sequence, DNA markers and genome mapping, flower color, mutations, flower opening, vase life, interspecific hybridization, fragrance.
The carnation is one of the most important ornamental flowers in the world, along with the chrysanthemum and the rose. The genus Dianthus is a member of the Caryophyllaceae and includes more than 300 species of annuals and evergreen perennials. Modern carnation cultivars are the product of highly complex hybridization, owing to their long history of breeding.
The carnation genome was first sequenced in ornamentals by a Japanese research team in 2013. The carnation has been genetically improved over the years, and there are various types of flower colors, shapes, patterns, and sizes. In this book, the molecular mechanism of flower color development and the transposable elements responsible for this diversity are studied in detail. In addition, it presents breeding and physiological research for improving flower vase life, one of the most important traits in ornamentals, based on a model of ethylene susceptible flowers. To improve selection efficiency, genomic analysis tools including DNA markers and genetic linkage maps are also highlighted. In closing, the book discusses mutation breeding technologies such as ion-beam irradiation and genetically modified carnations. -
Chapter 15 Dianthus, In: Ornamental Crops, Johan Van Huylenbroeck (ed.),P.349-381. Reviewed
Takashi Onozaki（ Role： Joint author）
Springer Nature 2018.07 （ ISBN:9783319906973 ）
Responsible for pages：349-381 Language：English Book type：Scholarly book
Carnation (Dianthus caryophyllus L.) is one of the world’s main floricultural crops. The genus Dianthus is a member of the Caryophyllaceae and includes more than 300 species of annuals and evergreen perennials. A very complex hybridization lies behind modern carnation cultivars owing to the species’ long history of breeding. In this chapter, the breeding history and transition of preferences for carnation cultivars (standard, spray, and dwarf types) are summarized. The chapter focuses on recent progress in carnation breeding research for flower color, mutations, flower type, disease resistance, vase life, interspecific hybridization, fragrance, and polyploidy. It also highlights the genetics of flower color and pigment composition in white cultivars, progress of breeding for resistance to bacterial wilt and for long vase life, and interspecific hybridization between carnation lines with long vase life and D. superbus var. longicalycinus, a wild species native to Japan, during the last 30 years of work by my research group. It also summarizes genomic analysis of carnation, including large-scale transcriptome sequencing using next-generation sequencing technology. It concludes by describing construction of a genetic linkage map, quantitative trait locus (QTL) analyses, and genome sequencing research.
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第２章 カーネーション 花の品種改良の日本史, P.31-62
小野崎 隆（ Role： Joint author）
悠書館, 東京 2016.06 （ ISBN:9784865820133 ）
Responsible for pages：31-62 Language：Japanese Book type：Scholarly book
日本におけるカーネーションの品種改良の歴史について、貢献度の高かった人物、社会的背景、公的機関、民間種苗会社および生産者による育種等にも触れながら、詳細に取りまとめた。
Misc 【 display / non-display 】
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ダリアエターニティシリーズの新品種‘エターニティムーン’と‘エターニティサンセット’の特徴およびエターニティシリーズ7品種の作型適応性等について
小野崎 隆
日本ダリア会会報 第21号 11 - 14 2025.03
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日持ち性に優れるダリア新品種エターニティシリーズ標準作業手順書 4品種追加拡充版 Reviewed
小野崎 隆
農研機構 標準作業手順書（SOP） 2025.01
Authorship：Lead author
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ダリアエターニティシリーズの新品種‘エターニティピーチ’、‘エターニティシャイン’の特徴および先行3品種の普及状況について
小野崎 隆
日本ダリア会会報 第20号 7 - 9 2024.03
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Breeding for the improvement of flower vase life in dahlia. 10. Breeding and characteristics of new dahlia cultivars ‘Eternity Moon’ and ‘Eternity Sunset’.
小野崎隆, 藤本卓生, 東未来
園芸学研究 別冊 23 ( 2 ) 2024
Industrial Property Rights 【 display / non-display 】
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トルコギキョウ植物を判別する方法、作出する方法、及びトルコギキョウ植物
川勝 恭子, 小野崎 隆, 佐藤 衛, 川勝 泰二, 福田 直子, 川部 眞登
Applicant：農研機構
Application no：特願2022-012657 Date applied：2022.01
Announcement no：特開2022-138114 Date announced：2022.09
Patent/Registration no：特許第7527665号 Date registered：2024.07
Rights holder：農研機構 Country of applicant：Domestic Country of acquisition：Domestic
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萎凋細菌病抵抗性カーネーションの 選抜に用いるオリゴヌクレオチド
小野崎 隆・谷川奈津・池田 広・柴田道夫
Applicant：農研機構
Application no：特願2003-011119 Date applied：2003.01
Announcement no：特開2004-222532 Date announced：2004.08
Patent/Registration no：特許第3855052号 Date registered：2006.09
Rights holder：農研機構 Country of applicant：Domestic Country of acquisition：Domestic
Other research activities 【 display / non-display 】
Honours, Awards and Prizes 【 display / non-display 】
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2020.03 「Breeding for Long Vase Life in Dahlia (Dahlia variabilis) Cut Flowers」
小野崎 隆，東 未来
Award type：Award from Japanese society, conference, symposium, etc.
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園芸学会 平成26年度 年間優秀論文賞
2014.03 「Expression and regulation of senescence-related genes in carnation flowers with low ethylene production during senescence」
棚瀬幸司, 大津佐和子, 佐藤 茂, 小野崎 隆
Award type：Award from Japanese society, conference, symposium, etc.
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園芸学会 平成19年度 年間優秀論文賞
2007.09 「Breeding of carnations (Dianthus caryophyllus L.) for long vase life and rapid decrease in ethylene sensitivity of flowers after anthesis」
小野崎隆，谷川奈津，八木雅史，池田廣，住友克彦，柴田道夫
Award type：Award from Japanese society, conference, symposium, etc.
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園芸学会 奨励賞
2005.04 「カーネーションの萎凋細菌病抵抗性および花持ち性の育種に関する研究」
小野崎 隆
Award type：Award from Japanese society, conference, symposium, etc.
Scientific Research Funds Acquisition Results 【 display / non-display 】
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新たな育種素材を利用した超長命性ダリアの育成と日持ち性向上のメカニズム解明
Grant number：24K08904 2024.04 - 2027.03
日本学術振興会 科学研究費助成事業 基盤研究(C)
小野崎 隆, 藤本卓生, 東 未来
Authorship：Principal investigator
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
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交雑育種によるダリアの日持ち性向上と良日持ちダリア系統の老化抑制機構の解析
Grant number：20K06026 2020.04 - 2024.03
日本学術振興会 科学研究費助成事業 基盤研究(C)
小野崎 隆, 東 未来
Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）
日持ち性による選抜と交配を3回行った第4世代等一次選抜系統86系統の日持ち性を再評価し、20系統を二次選抜した。第4世代選抜系統003-15の冬春期栽培の蒸留水での平均日持ち日数は、従来品種‘かまくら’の6.3日に対し、15.1日と超長命性を示し、高温下（28℃・GLA液）の平均日持ち日数も、系統003-15では11.2日と優れていた。さらに、良日持ち性品種化候補の2系統が得られた。2021年3月30日に良日持ち性系統交雑種子および自然結実種子合計1307粒を播種し、第5世代76個体、第4世代28個体を含む388個体を露地圃場へ定植した。9月10日までに開花した322実生の日持ち性を評価し、第5世代31系統を含む58系統を一次選抜した。第5世代、第4世代の平均日持ち日数は、それぞれ8.0日、6.4日と、第3世代の6.2日から、それぞれ0.2日、1.8日増加した。
良日持ちダリア系統の老化抑制機構を明らかにするため、2021年度に部分長配列を明らかにした4遺伝子（NAC4-4、NAC6-2、NAC7-4、NAC9-8）の発現解析を花のステージ別に行った。良日持ちダリア6系統と従来品種 ‘かまくら’を用い、開花2日前の蕾、開花日から開花後10日までの最外側の花弁をサンプルとし、リアルタイムPCRによる発現解析を行った。その結果、‘かまくら’では3～5日、良日持ちダリア系統はいずれも7～9日でNAC4-4、NAC6-2、NA9-8の発現量が増加した。一方で、NAC7-4は開花日と比較して顕著な変化は見られなかった。以上の結果から、良日持ちダリア系統ではNAC4-4、NAC6-2、NAC9-8等のNAC遺伝子の発現時期が、‘かまくら’よりも遅れることが明らかとなった。NAC遺伝子の発現の遅延が、良日持ちダリア系統の日持ちの良さの一因である可能性が考えられる。 -
Clarification of ethylene-response mechanism using bud mutation in carnation.
Grant number：19K06043 2019.04 - 2022.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
TANASE Koji
Grant amount：\2990000 （ Direct Cost: \2300000 、 Indirect Cost：\690000 ）
In carnations, there are varieties with a reduced response to ethylene, which character is less susceptible to wilting in response to exogenous ethylene. We found a mutant with "normal ethylene response" red petals and "reduced ethylene response" orange petals within a single flower. We extracted RNA from the orange and red petals, respectively, and analyzed them by next-generation sequencing. Approximately 32,500 genes were identified for each petals. We extracted 503 genes whose expression levels differed more than 2-fold between red and orange petals. These genes include anthocyanin synthesis genes as well as transporters and other genes.
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中課題「日持ち性等に優れた性質を持つ新規有望品目の育成」 小野崎 隆
2015.04 - 2020.03
「国産花きの国際競争力強化のための技術開発」 農林水産省 委託プロジェクト研究
中山真義
Grant type：Competitive