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特命准教授 |
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東京都世田谷区桜丘１−１−１８号館５階 |
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- 特命准教授 |
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Nodai Research Institute 特命准教授
2022.04
External Career 【 display / non-display 】
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Tokyo University of Agriculture Laboratory of Yeast Systematics, Nodai Research Institute Specially Appointed Associate Professor Ph.D.
2022.04
Country：Japan
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Tokyo University of Agriculture PhD researcher
2021.04
Country：Japan
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National Institute of Genetics Microbial Physiology, Department of Gene Function and Phenomics Assistant Professor
2019.01 - 2021.03
Country：Japan
Professional Memberships 【 display / non-display 】
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日本医真菌学会
2022.06
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酵母遺伝学フォーラム
2010.07
Research Areas 【 display / non-display 】
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Life Science / Cell biology / Biodiversity using basidiomycetous yeast
Papers 【 display / non-display 】
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Whole-genome based phylogeny and comparative genomics of Sporidiobolales and related taxa of Basidiomycetes Reviewed
Yuuki Kobayashi, Naoto Tanaka, Minenosuke Matsutani, Yuuna Kurokawa, Keita Aoki, Moriya Ohkuma, Ri-ichiroh Manabe, Masako Takashima
IMA Fungus 16 ( e141626 ) 2025.05
Language：English Publishing type：Research paper (scientific journal)
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Analyses of hyphal diversity in Trichosporonales yeasts based on fluorescent microscopic observations. Reviewed International journal
Keita Aoki, Moriya Ohkuma, Takashi Sugita, Yuuki Kobayashi, Naoto Tanaka, Masako Takashima
Microbiology spectrum e0321024 2025.02
Language：English Publishing type：Research paper (scientific journal)
In dimorphic yeasts, hyphal growth is primarily associated with infection and mycosis progression, with Trichosporon asahii causing deep-seated mycosis and summer-type hypersensitivity pneumonitis. Magnesium accelerates hyphal growth in T. asahii, leading to multi-septation, vacuolar expansion, and decreased lipid droplet size. However, the commonality of these phenotypes has not been studied in Trichosporonales yeasts. Therefore, to explore whether similar magnesium-induced phenotypes occur across Trichosporonales yeasts, we examined hyphal growth, multi-septation, vacuolar extension, and lipid droplet size and number in 30 species. Cell length increased with magnesium treatment in 13 yeasts: 5 Trichosporon (T. asahii, Trichosporon aquatile, Trichosporon asteroides, Trichosporon coremiiforme, and Trichosporon ovoides), three Apiotrichum (Apiotrichum brassicae, Apiotrichum montevideense, and Apiotrichum veenhuisii), three Cutaneotrichosporon (Cutaneotrichosporon cavernicola, Cutaneotrichosporon cutaneum, and Cutaneotrichosporon dermatis), Pascua guehoae, and Takashimella koratensis. C. dermatis also underwent pseudo-hyphal growth. Multi-septation increased in seven dimorphic yeasts, including five Trichosporon spp., Trichosporon faecale, and C. dermatis. The vacuolar area was significantly extended in T. asahii, T. aquatile, T. ovoides, and C. dermatis. Lipid droplet size increased only in Trichosporon inkin; however, it decreased in T. asahii, T. coremiiforme, and T. faecale. Additionally, lipid droplet number was preferentially altered in Apiotrichum and Cutaneotrichosporon. These results suggested that magnesium-induced multi-septation and vacuolar area expansion phenotypically distinguish Trichosporon hyphae from Apiotrichum and Cutaneotrichosporon hyphae and distinguish C. dermatis pseudo-hyphae from Cutaneotrichosporon spp. Moreover, differences in lipid droplet size can discriminate species within Trichosporon. Our results suggest that phenotypic alteration via magnesium treatment can contribute to the characterization of Trichosporonales yeasts. These findings provide insights into fungal pathogenesis and may support new treatment strategies.IMPORTANCEMagnesium sulfate considerably affects hyphal growth and cellular organization in Trichosporon asahii. To examine the commonality of this phenotype in Trichosporonales, we treated 30 Trichosporonales yeasts with magnesium sulfate and observed genus-level phenotypic alterations. Using cell length measurement, lipid droplet staining, septum staining, and vacuole staining, considerable hyphal diversity was observed among Trichosporonales. Notably, differences in the multi-septation phenotype and vacuolar size distinguished Trichosporon hyphae from Apiotrichum and Cutaneotrichosporon hyphae and distinguished Cutaneotrichosporon dermatis from other Cutaneotrichosporon spp. Moreover, differences in lipid droplet phenotype divided Trichosporon hyphae into two groups. Our study revealed the relationship between hyphal morphology and phylogenetic systematics in Trichosporonales.
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Yuuki Kobayashi, Ayane Kayamori, Keita Aoki, Yuh Shiwa, Minenosuke Matsutani, Nobuyuki Fujita, Takashi Sugita, Wataru Iwasaki, Naoto Tanaka, Masako Takashima
BMC Genomics 24 ( 1 ) 2023.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
Abstract
Background
Since DNA information was first used in taxonomy, barcode sequences such as the internal transcribed spacer (ITS) region have greatly aided fungal identification; however, a barcode sequence alone is often insufficient. Thus, multi-gene- or whole-genome-based methods were developed. We previously isolated Basidiomycota yeasts classified in the Trichosporonales. Some strains were described as Cutaneotrichosporon cavernicola and C. spelunceum, whereas strain HIS471 remained unidentified. We analysed the genomes of these strains to elucidate their taxonomic relationship and genetic diversity.
Results
The long-read-based assembly resulted in chromosome-level draft genomes consisting of seven chromosomes and one mitochondrial genome. The genome of strain HIS471 has more than ten chromosome inversions or translocations compared to the type strain of C. cavernicola despite sharing identical ITS barcode sequences and displaying an average nucleotide identity (ANI) above 93%. Also, the chromosome synteny between C. cavernicola and the related species, C. spelunceum, showed significant rearrangements, whereas the ITS sequence identity exceeds 98.6% and the ANI is approximately 82%. Our results indicate that the relative evolutionary rates of barcode sequences, whole-genome nucleotide sequences, and chromosome synteny in Cutaneotrichosporon significantly differ from those in the model yeast Saccharomyces.
Conclusions
Our results revealed that the relative evolutionary rates of nucleotide sequences and chromosome synteny are different among fungal clades, likely because different clades have diverse mutation/repair rates and distinct selection pressures on their genomic sequences and syntenic structures. Because diverse syntenic structures can be a barrier to meiotic recombination and may lead to speciation, the non-linear relationships between nucleotide and synteny diversification indicate that sequence-level distances at the barcode or whole-genome level are not sufficient for delineating species boundaries.DOI： 10.1186/s12864-023-09718-2
Other Link： https://link.springer.com/article/10.1186/s12864-023-09718-2/fulltext.html
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Hyphal Growth in <i>Trichosporon asahii</i> Is Accelerated by the Addition of Magnesium Reviewed
Keita Aoki, Kosuke Yamamoto, Moriya Ohkuma, Takashi Sugita, Naoto Tanaka, Masako Takashima
Microbiology Spectrum 11 ( 3 ) 2023.06
Language：English Publishing type：Research paper (scientific journal) Publisher：American Society for Microbiology
Understanding the mechanism underlying fungal dimorphism is crucial to discern its invasion into human cells. Invasion is caused by the hyphal form rather than the yeast form; therefore, it is important to understand the mechanism of transition from the yeast to hyphal form.
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A genome-informed higher rank classification of the biotechnologically important fungal subphylum Saccharomycotina Reviewed International coauthorship
M. Groenewald, C.T. Hittinger, K. Bensch, D.A. Opulente, X.-X. Shen, Y. Li, C. Liu, A.L. LaBella, X. Zhou, S. Limtong, S. Jindamorakot, P. Gonçalves, V. Robert, K.H. Wolfe, C.A. Rosa, T. Boekhout, N. Čadež, G. Péter, J.P. Sampaio, M.-A. Lachance, A.M. Yurkov, H.-M. Daniel, M. Takashima, K. Boundy-Mills, D. Libkind, K. Aoki, T. Sugita, A. Rokas
Studies in Mycology 105 1 - 22 2023.06
Language：English Publishing type：Research paper (scientific journal)
Books and Other Publications 【 display / non-display 】
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国際菌学会（IMC12）および国際酵母学会（ICY16）で開催されたThe Yeasts, A Taxonomic Studyの更新版発行に関するミーティング
青木敬太, 小林裕樹, 高島昌子（ Role： Joint author , 40 (2): 1-4）
日本微生物資源学会誌 2024
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微生物資源の整備と利活用の戦略
青木敬太, 小林裕樹, 高島昌子（ Role： Contributor , 第４章第２節 真菌の命名と系統分類に関する課題）
エヌ・ティー・エス 2023.09 （ ISBN:9784860438586 ）
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染色体サイクル ゲノム恒常性維持、継承とダイナイクス内の概論「染色体の分配とM期制御」
青木敬太、仁木宏典（ Role： Joint author）
共立出版 2009
Language：Japanese Book type：Scholarly book
Misc 【 display / non-display 】
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Analyses on genomic evolution and speciation of Basidiomycota yeasts based on whole-genome information
小林裕樹, 栢森綺音, 青木敬太, 志波優, 松谷峰之介, 藤田信之, 杉田隆, 岩崎渉, 大熊盛也, 田中尚人, 高島昌子
日本分子生物学会年会プログラム・要旨集(Web) 46th 2023
Scientific Research Funds Acquisition Results 【 display / non-display 】
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新規モデル生物を用いた核膜動態の研究
2013.04 - 2014.03
科学研究費補助金 上原記念生命科学財団研究奨励金
青木敬太
Other External Funds 【 display / non-display 】
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新規モデル生物を用いた核膜動態の研究
2013.04 - 2014.03
財団
青木敬太
Grant type：Competitive
Presentations 【 display / non-display 】
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担子菌酵母Trichosporon asahiiのニ形性に関与する遺伝子機能の研究
青木敬太, 松谷峰之介, 大熊盛也, 杉田隆, 小林裕樹, 田中尚人, 高島昌子
第47回日本分子生物学会年会 2024.11
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Dimorphic transition between yeast, hypha, and arthroconidia morphologies by Trichosporon asahii Invited
Keita Aoki, Minenosuke Matsutani, Moriya Ohkuma, Takashi Sugita, Yuuki Kobayashi, Naoto Tanaka, Masako Takashima
The 8th Congress of Asia-Pacific Society for Medical Mycology (APSMM 2024) 2024.11
Event date： 2024.11
Language：English Presentation type：Oral presentation (invited, special)
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蛍光顕微鏡観察によって明らかになるトリコスポロン酵母の性状 Invited
青木敬太, 大熊盛也, 杉田隆, 小林裕樹, 田中尚人, 高島昌子
第68回日本医真菌学会総会・学術集会 2024.11
Event date： 2024.11
Language：Japanese Presentation type：Oral presentation (invited, special)
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Transition from hyphae to arthroconidia requires a nucleolar protein NOP14 ortholog in Trichosporon asahii
Keita Aoki, Minenosuke Matsutani, Moriya Ohkuma, Takashi Sugita, Yuuki Kobayashi, Naoto Tanaka, Masako Takashima
16th International Congress on Yeasts 2024.10
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蛍光顕微鏡観察に基づいたトリコスポロン酵母の種間多様性解析
青木敬太, 大熊盛也, 杉田隆, 小林裕樹, 田中尚人, 高島昌子
日本微生物資源学会第30回 2024.07
Teaching Experience 【 display / non-display 】
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分子細胞生物学（染色体分配）
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酵母ゲノム生物学の基盤と応用
Committee Memberships 【 display / non-display 】
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生物遺伝資源に関する大腸菌小委員会およびNBRP原核生物運営委員会（オブザーバー）
2011.08 - 2021.03