Updated on 2024/03/13


Hiromasa Saitoh




Laboratory Address

Departmant of Molecular Biology, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan

Laboratory Phone number


Contact information

Contact information

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From School 【 display / non-display

  • Teikyo University   Faculty of Science and Engineering   Graduated

    1989.04 - 1993.03

From Graduate School 【 display / non-display

  • Osaka Prefecture University   Graduate School, Division of Agriculture   Doctoral program (second term)   Accomplished credits for doctoral program

    1995.04 - 1998.06

  • Mie University   Graduate School, Division of Bioresources   Doctoral program (first term)   Completed

    1993.04 - 1995.03

Degree 【 display / non-display

  • 博士(農学) ( 1999.03   大阪府立大学 )

  • 修士(生物資源学) ( 1995.03   三重大学 )

Employment Record in Research 【 display / non-display

  • Tokyo University of Agriculture   Faculty of Life Sciences   Department of Molecular Microbiology   Professor


External Career 【 display / non-display

  • (公財)岩手生物工学研究センター   主任研究員

    2010.03 - 2017.03

  • (財)岩手生物工学研究センター   ポスドク研究員

    2005.04 - 2010.02

  • Max Planck Institute for Plant Breeding Research(ケルン、ドイツ)   ポスドク研究員

    2003.01 - 2005.03

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  • (財)岩手生物工学研究センター   日本学術振興会特別研究員

    2002.04 - 2002.12

  • (財)岩手生物工学研究センター   科学技術振興事業団科学技術特別研究員

    2001.01 - 2002.03

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Professional Memberships 【 display / non-display

  • 日本植物病理学会


Research Areas 【 display / non-display

  • Others / Others  / 植物病理学

Research Interests 【 display / non-display

  • 遺伝学

  • 生化学

  • 分子生物学

Papers 【 display / non-display

  • Barley MLA3 recognizes the host-specificity effector Pwl2 from Magnaporthe oryzae Reviewed International coauthorship

    Brabham, H.J., De La Cruz, D.G., Were, V., Shimizu, M., Saitoh, H., et al.

    THE PLANT CELL   36 ( 2 )   447 - 470   2024.01

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Society of Plant Biologists  


    DOI: 10.1093/plcell/koad266

    Other Link: https://academic.oup.com/plcell/article/36/2/447/7308434

  • Disentangling the complex gene interaction networks between rice and the blast fungus identifies a new pathogen effector

    Sugihara Yu, Abe Yoshiko, Takagi Hiroki, Abe Akira, Shimizu Motoki, Ito Kazue, Kanzaki Eiko, Oikawa Kaori, Kourelis Jiorgos, Langner Thorsten, Win Joe, Białas Aleksandra, Lüdke Daniel, Contreras Mauricio P., Chuma Izumi, Saitoh Hiromasa, Kobayashi Michie, Zheng Shuan, Tosa Yukio, Banfield Mark J., Kamoun Sophien, Terauchi Ryohei, Fujisaki Koki

    PLOS Biology   21 ( 1 )   e3001945   2023.01

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    Studies focused solely on single organisms can fail to identify the networks underlying host–pathogen gene-for-gene interactions. Here, we integrate genetic analyses of rice (Oryza sativa, host) and rice blast fungus (Magnaporthe oryzae, pathogen) and uncover a new pathogen recognition specificity of the rice nucleotide-binding domain and leucine-rich repeat protein (NLR) immune receptor Pik, which mediates resistance to M. oryzae expressing the avirulence effector gene AVR-Pik. Rice Piks-1, encoded by an allele of Pik-1, recognizes a previously unidentified effector encoded by the M. oryzae avirulence gene AVR-Mgk1, which is found on a mini-chromosome. AVR-Mgk1 has no sequence similarity to known AVR-Pik effectors and is prone to deletion from the mini-chromosome mediated by repeated Inago2 retrotransposon sequences. AVR-Mgk1 is detected by Piks-1 and by other Pik-1 alleles known to recognize AVR-Pik effectors; recognition is mediated by AVR-Mgk1 binding to the integrated heavy metal-associated (HMA) domain of Piks-1 and other Pik-1 alleles. Our findings highlight how complex gene-for-gene interaction networks can be disentangled by applying forward genetics approaches simultaneously to the host and pathogen. We demonstrate dynamic coevolution between an NLR integrated domain and multiple families of effector proteins.

    DOI: 10.1371/journal.pbio.3001945

  • Rice apoplastic CBM1-interacting protein counters blast pathogen invasion by binding conserved carbohydrate binding module 1 motif of fungal proteins Reviewed

    Takeda, T., Takahashi, M., Shimizu, M., Sugihara, Y., Yamashita, T., Saitoh, H., Fujisaki, K., Ishikawa, K., Utsushi, H., Kanzaki, E., Sakamoto, Y., Abe, A. and Terauchi, R.

    PLOS Pathogens   18 ( 9 )   e1010792   2022.09

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    Language:English   Publishing type:Research paper (scientific journal)  

    When infecting plants, fungal pathogens secrete cell wall-degrading enzymes (CWDEs) that break down cellulose and hemicellulose, the primary components of plant cell walls. Some fungal CWDEs contain a unique domain, named the carbohydrate binding module (CBM), that facilitates their access to polysaccharides. However, little is known about how plants counteract pathogen degradation of their cell walls. Here, we show that the rice cysteine-rich repeat secretion protein OsRMC binds to and inhibits xylanase MoCel10A of the blast fungus pathogen Magnaporthe oryzae, interfering with its access to the rice cell wall and degradation of rice xylan. We found binding of OsRMC to various CBM1-containing enzymes, suggesting that it has a general role in inhibiting the action of CBM1. OsRMC is localized to the apoplast, and its expression is strongly induced in leaves infected with M. oryzae. Remarkably, knockdown and overexpression of OsRMC reduced and enhanced rice defense against M. oryzae, respectively, demonstrating that inhibition of CBM1-containing fungal enzymes by OsRMC is crucial for rice defense. We also identified additional CBM-interacting proteins (CBMIPs) from Arabidopsis thaliana and Setaria italica, indicating that a wide range of plants counteract pathogens through this mechanism.

    DOI: 10.1371/journal.ppat.1010792

  • A genetically linked pair of NLR immune receptors shows contrasting patterns of evolution Reviewed

    Shimizu, M., Hirabuchi, A., Sugihara, Y., Abe, A., Takeda, T., Kobayashi, M., Hiraka, Y., Kanzaki, E., Oikawa, K., Saitoh, H., Langner, T., Banfield, MJ., Kamoun, S. and Terauchi, R.

    Proceedings of the National Academy of Sciences of the United States of America   119 ( 27 )   e2116896119   2022.06

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.2116896119

  • Multiple variants of the fungal effector AVR-Pik bind the HMA domain of the rice protein OsHIPP19, providing a foundation to engineer plant defense Reviewed International journal

    Madiment, JHR., Franceschetti, M., Maqbool, A., Saitoh, H., Jantasuriyarat, C., Kamoun, S., Terauchi, R. and Banfield, MJ.

    Journal of Biological Chemistry   296 ( 100371 )   1 - 13   2021.02

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ASBMB PUBLICATIONS  


    DOI: 10.1016/j.jbc.2021.100371

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Books and Other Publications 【 display / non-display

  • Applied Plant Biotechnology for Improving Resistance to Biotic Stress

    Fujisaki, K., Shimizu, M., Oikawa, K., Takeda, T., Takagi, H., Abe, A., Okuyama, Y., Yoshida, K. and Saitoh, H.( Role: Joint author ,  共同研究につき抽出不可能)

    Academic Press  2019.10  ( ISBN:978-012-81630-5

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    Total pages:355   Responsible for pages:181-188   Language:Japanese   Book type:Scholarly book


  • Advances in the Understanding of Biological Sciences Using Next Generation Sequencing (NGS) Approaches.

    Terauchi, R., …… Saitoh, H., et al. Whole genome sequencing to identify genes and QTL in rice.( Role: Joint author ,  共同研究につき担当分抽出不可能)

    Springer  2015.09 

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    Total pages:241   Responsible for pages:33-41   Language:English   Book type:Scholarly book

    Whole genome sequencing to identify genes and QTL in rice.

  • 感染と防御をめぐる新潮流

    齋藤宏昌ら( Role: Joint author ,  共同研究につき担当分抽出不可能)

    植物感染生理談話会論文集、第50号  2015.08 

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    Total pages:152   Responsible for pages:41-46   Language:Japanese   Book type:Scholarly book


  • Plant-Pathogen Interactions

    Kanzaki, H., Yoshida, K., Saitoh, H., et al.( Role: Joint author ,  共同研究につき担当分抽出不可能)

    Methods Mol. Biol. 1127巻  2014.02 

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    Total pages:306   Responsible for pages:269-275   Language:English   Book type:Scholarly book

    第20章 Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice.

  • 植物−病原微生物の相互作用研究の新展開

    寺内良平・……・齋藤宏昌( Role: Joint author ,  共同研究につき担当分抽出不可能)

    植物感染生理談話会論文集、第47号   2012.08 

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    Total pages:155   Responsible for pages:79-85   Language:Japanese   Book type:Scholarly book


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Industrial Property Rights 【 display / non-display

  • 多糖結合部位認識タンパク質の製造方法,多糖結合部位を有するタンパク質の単離方法,多糖結合部位を有するタンパク質の機能抑制方法,植物病原菌の防除方法


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    Application no:2014-255544  Date applied:2014.12

  • セルロース分解を促進するタンパク質及びその利用とその生産方法


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    Announcement no:2010-172206  Date announced:2010.08

  • 新規な植物細胞死誘導因子NbCD3


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    Patent/Registration no:4583051  Date registered:2010.09  Date issued:2010.09

    N. benthamianaを用いたハイスループット遺伝子発現選抜法により同定された新規細胞死誘導因子MAPKKK(NbCD3)の同定

  • 新規な植物細胞死誘導因子NbCD2


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    Patent/Registration no:4583050  Date registered:2010.09  Date issued:2010.09

    ハイスループットin planta遺伝子発現選抜による植物細胞死および非宿主抵抗性を制御するRNA recognition motif(RRM)に属するタンパク質NbCD2の同定

  • 新規な植物細胞死誘導因子NbCD1


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    Patent/Registration no:4776216  Date registered:2011.07  Date issued:2011.07

    ハイスループットin planta遺伝子発現選抜による植物細胞死および非宿主抵抗性を制御するクラスⅡエチレン反応性エレメント結合因子様タンパク質NbCD1の同定

Scientific Research Funds Acquisition Results 【 display / non-display

  • イネいもち病抵抗性NLRタンパク質による病原菌エフェクター認識の分子機構の解明

    2018.04 - 2021.03

    科学研究費補助金  基盤研究(C)


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    Authorship:Principal investigator 

  • イネーいもち病菌相互作用の分子機構解明

    2016.04 - 2017.03

    科学研究費補助金  基盤研究(S)

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  • 植物ー病原菌相互作用の集団ゲノミックス解析

    2011.04 - 2016.03

    科学研究費補助金  新学術領域研究

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  • いもち病菌感染イネにおけるエフェクターとそのターゲットの相互作用の解明

    2010.04 - 2013.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

Other External Funds 【 display / non-display

  • 北東北地域向け非主食用多用途稲の直播品種及び直播栽培等関連技術の開発

    2010.04 - 2012.03


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    Authorship:Coinvestigator(s)  Grant type:Competitive

Presentations 【 display / non-display

  • RNA-seq of in planta-expressed Magnaporthe oryzae genes identifies MoSVP as a highly expressed genes required for pathogenicity at the initial stage of infection Invited International conference

    Hiromasa Saitoh

    The 6th International Conference on Agricultural and Biological Sciences (ABS 2020)  2020.08  ABS 2020 Conference Organizing Committee

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    Event date: 2020.08

    Language:English   Presentation type:Oral presentation (invited, special)  



  • エフェクター分泌によるいもち病菌の感染および抵抗性誘導機構 Invited


    第3回 植物病理を紡ぐ会  2019.03  “植物病理を紡ぐ会” 世話人一同

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    Event date: 2019.03

    Language:Japanese   Presentation type:Oral presentation (invited, special)  



  • Analysis of rice blast infection and resistance-inducing mechanisms via effectors secreted from Magnaoporthe oryzae. International conference

    Saitoh, H., Kanzaki, H., Fujisaki, K. and Terauchi, R.

    2015 KSM Spring Meeting & KSM-ICWG-GSP Joint Clubroot Symposium  2015.05  KSM Spring Meeting & KSM-ICWG-GSP Joint Clubroot Symposium

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    Event date: 2015.05

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Daejeon Convention Center, Daejeon, Korea  


  • イネいもち病菌AVR-Pik、AVR-Piiの分泌・宿主細胞内移行の解明


    平成26年度日本植物病理学会大会  2014.06  日本植物病理学会

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    Event date: 2014.06

    Language:Japanese   Presentation type:Oral presentation (general)  



  • イネいもち病菌の胞子生産と菌糸のメラニン化に関与するヘキソーストランスポーター様タンパク質遺伝子MoST1


    平成25年度日本植物病理学会東北部会  2013.10  日本植物病理学会東北部会

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    Event date: 2013.10

    Language:Japanese   Presentation type:Oral presentation (general)  



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