|
Title |
Professor |
|
External Link |
|
|
|
SAGANE Yoshimasa Professor |
Studying abroad experiences 【 display / non-display 】
-
2003.04 - 2011.09 Sars International Centre for Marine Molecular Biology 博士研究員
Employment Record in Research 【 display / non-display 】
-
Tokyo University of Agriculture Faculty of Bio-Industry -
2001.04 - 2003.03
-
Tokyo University of Agriculture Faculty of Bio-Industry Department of Food and Cosmetic Science Associate Professor
2011.09 - 2014.03
-
Tokyo University of Agriculture Faculty of Bio-Industry Department of Food and Cosmetic Science Professor
2014.04 - 2018.03
-
Tokyo University of Agriculture Faculty of Bio-Industry Department of Food, Aroma and Cosmetic Chemistry Professor
2018.04
External Career 【 display / non-display 】
-
Sars International Centre for Marine Molecular Biology 研究員
2003.04 - 2011.09
Country：Norway
Professional Memberships 【 display / non-display 】
-
日本分子生物学会
2018
-
食香粧研究会
2012
-
日本細菌学会
2012
-
日本生化学会
2012
Research Areas 【 display / non-display 】
-
Life Science / Bacteriology
-
Life Science / Structural biochemistry
Research Interests 【 display / non-display 】
-
Alternative protein
-
Protein chemistery
-
Clostridium botulinum
-
Botulinum toxin complex
-
Molecular Biology
Papers 【 display / non-display 】
-
ボツリヌス毒素の構造と機能解析から導かれる応用可能性 Invited
相根義昌、宮下慎一郎
グリーンテクノ情報 19 ( 4 ) 15 - 19 2024.03
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Research paper (bulletin of university, research institution) Publisher：NPO法人グリーンテクノバンク
-
Characterization of Serotype CD Mosaic Botulinum Neurotoxin in Comparison with Serotype C and A Reviewed International coauthorship International journal
Shin-Ichiro Miyashita , Shura Karatsu , Mako Fujiishi , I Husn Huang , Yuki Nagashima , Tamaki Morobishi , Keita Hosoya , Tsuyoshi Hata , Min Dong , Yoshimasa Sagane
Toxins 15 ( 2 ) 123 2023.02
Authorship：Last author Language：English Publishing type：Research paper (scientific journal)
Botulinum neurotoxin (BoNT), produced by Clostridium botulinum, cleaves proteins involved in neurotransmitter release, thereby triggering flaccid paralyses, which are responsible for botulism. BoNT is classified into seven serotypes (BoNT/A-G); BoNT/A and BoNT/B are used as medical therapeutics and anti-wrinkle reagents. In this study, we investigated the efficacy of BoNT/CD, a mosaic toxin of BoNT/C and BoNT/D, to assess its potential as a therapeutic alternative for BoNT/A. In a cultured neuron assay, BoNT/CD cleaved syntaxin and SNAP-25 with higher efficacy than BoNT/C and BoNT/A. Intramuscularly administrated BoNT/CD induced dose-dependent muscle paralysis, and the paralysis lasted ~21 days in a mouse digit abduction score assay (BoNT/A-induced paralysis lasted ~30 days). BoNT/C failed to induce local paralysis without systemic toxicity. Multiple alignment analyses of the amino acid sequences of the receptor binding domain (HC) of eight BoNT/CDs and two BoNT/Ds showed sequence clustering in five groups. Comparing BoNT/CD strain 003-9 (BoNT/CD003-9) and strain 6813 (BoNT/CD6813) showed that both BoNT/CDs displayed similar efficacies in cultured neurons, but BoNT/CD003-9 displayed higher efficacy in a mouse model than BoNT/CD6813. These findings suggest that BoNT/CD may be a potential alternative for patients who do not respond to existing BoNT-based therapeutics.
-
Variants of hemagglutinin-33 in the serotype C botulinum progenitor toxin complex boost its oral toxicity Reviewed
Karatsu Shura, Miyashita Sin-Ichiro, Hata Tsuyoshi, Huang I Hsun, Hosoya Keita, Sagane Yoshimasa
49 ( 1 ) 19 - 28 2023.01
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
-
Development of “Yezo Venison Sausage” with improved texture and bloody taste quality
Nakazawa Yozo, Morino Tatsuya, Miyashita Shin-ichiro, Minami Kazuhiro, Sagane Yoshimasa
Nippon Shokuhin Kagaku Kogaku Kaishi advpub ( 0 ) 2023
Language：Japanese Publisher：Japanese Society for Food Science and Technology
<p>We developed “Yezo Venison Sausage” using the characteristics of Yezo sika deer meat to overcome disadvantages associated with the unique smell and tough texture of deer meat to turn Yezo sika deer, a Hokkaido-specific biological resource, into a beneficial resource. “Yezo Venison Sausage” is produced by the addition of 20% minced pork fat, 20% crushed ice, 2.4% salt, 2% sugar, 0.15% color developer, 0.1% ascorbic acid, 0.2% polyphosphate, 0.4% baking soda, 0.6% coarse ground black pepper, 0.2% all spice, and 0.1% sage to minced Yezo sika deer thigh. The ingredients are mixed by hand and then filled into casings made of sheep intestine. After drying at 60°C for 30 min, the sausage is smoked at 60°C for 30 min and then cooked at a central temperature of 70°C for 1 min. The newly developed “Yezo Venison Sausage” shows greatly improved texture and bloody taste quality. Further, the high protein, low fat, and high iron contents, all of which are distinctive characteristics of deer meat, along with the distinctive redness of the meat confer an excellent balance between the sensory and nutritional characteristics of the sausage. </p>
-
Species-specific gene duplication in Clostridia produces variations of cholesterol-dependent cytolysin with different cytotoxicity Reviewed International journal
Keita Hosoya, Shin-Ichiro Miyashita, Shura Karatsu, I-Hsun Huang, Yoshimasa Sagane
Biochemical and Biophysical Research Communications 637 ( 11 ) 240 - 246 2022.12
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
Books and Other Publications 【 display / non-display 】
-
生食のはなし
川本 伸一(編集代表)／朝倉 宏・稲津 康弘・畑江 敬子・山﨑 浩司(編)（ Role： Contributor , 危害要因コラム③ボツリヌス）
朝倉書店 2023.04 （ ISBN:978-4-254-43130-8 ）
Total pages：160 Responsible for pages：105-106 Language：Japanese Book type：Textbook, survey, introduction
肉や魚などを加熱せずに食べる「生食」の文化や注意点をわかりやすく解説。調理現場や家庭で活用しやすいよう食材別に章立てし，実際の食中毒事例をまじえつつ危険性や対策を紹介。
-
フレーバー・クリエーション
ジョン・ライト（著） 藤森 嶺、和智 進一、寺本 明子、相根 義昌（訳）（ Role： Joint translator）
講談社 2014.05
Language：Japanese Book type：Scholarly book
-
Botulinum Toxin Complex: A Delivery Vehicle of Botulinum Neurotoxin Traveling Digestive Tract
Sagane, Y., Inui, K., Miyashita S, -I., Miyata, K., Suzuki, T., Niwa, K., Watanabe, T.（ Role： Joint author）
InTech 2012.08
Language：English Book type：Scholarly book
-
Structure and function of the Clostridium botulinum serotypes C and D toxin complex. In: Current Topics in Food Science and Technology.
Watanabe, T., Sagane, Y., Mutoh, S., Suzuki, T., Hasegawa, K., Kouguchi, H., and Ohyama, T. (Nagai, T., Suzuki, N., and Nagashima, T. eds) （ Role： Joint author）
Research Signpost 2005
Language：English Book type：Scholarly book
ボツリヌスCおよびD型毒素の構造と機能に関する研究成果をまとめた
-
初歩からのバイオ実験
大山徹、渡部俊弘 編著 石井正治、鎌口有秀、川崎信治、川本尋義、相根義昌、高野克己、中川智行、新村洋一、西澤信、桃木芳枝、山川隆、横田博、横浜道成 共著（ Role： Joint author）
三共出版 2002.05
Language：Japanese Book type：Textbook, survey, introduction
分子生物学研究に必要な基礎実験のプロトコールを図入りでまとめた解説書
Industrial Property Rights 【 display / non-display 】
-
血管内皮機能を向上する作用を有する剤又は食品
南 和広, 樫村 修生, 丹羽 光一, 相根 義昌, 渡部 俊弘, 前崎 祐二, 清柳 典子
Application no：特願2017-113985 Date applied：2017.06
Announcement no：特開2018-203695 Date announced：2018.12
Country of applicant：Domestic
-
アセチルコリンエステラーゼ活性を有するタンパク質、その精製方法および遺伝子
桃木 芳枝, 相根 義昌
Application no：特願2003-154226 Date applied：2003.05
Announcement no：特開2004-350616 Date announced：2004.12
Country of applicant：Domestic
Scientific Research Funds Acquisition Results 【 display / non-display 】
-
経口毒性が強化された変異型ボツリヌス毒素複合体の機能解析
2022.04 - 2025.03
科学研究費補助金 基盤研究(C)
-
新規に見出されたボツリヌス毒素複合体の細胞認識における「機能シフト機構」の解明
2018.04 - 2021.03
科学研究費補助金 基盤研究(C)
相根義昌
Authorship：Principal investigator
-
ボツリヌス神経毒素結合タンパク質NTNHAの体内侵入に寄与する生体分子の解明
2015.04 - 2018.03
科学研究費補助金 基盤研究(C)
相根義昌
Authorship：Principal investigator
-
ボツリヌス神経毒素結合タンパク質NTNHAの細胞層透過メカニズム
2013.04 - 2015.03
科学研究費補助金 若手研究(B)
相根義昌
Authorship：Principal investigator
Presentations 【 display / non-display 】
-
長芋パウダーはアイスクリームの安定化剤になるか？ International conference
中澤洋三、村田雪乃、宮下慎一郎、南和広、相根義昌
日本食品保蔵科学会大会講演要旨集 2022.06
Event date： 2022.06
Language：Japanese Presentation type：Oral presentation (general)
-
抗体医薬を用いた感染制御戦略 ボツリヌス神経毒素を利用した神経細胞への抗体送達によるボツリヌス中毒の治療 Invited International conference
宮下 慎一郎, Zhang Jie, Zhang Sicai, 相根 義昌, Shoemaker Charles, Dong Min
日本細菌学雑誌 2022.02
Event date： 2022.02
Language：English Presentation type：Oral presentation (general)
-
ボツリヌス毒素複合体の上皮細胞への侵入経路の解明 International conference
HUANG I Hsun , 宮下慎一郎 , 唐津修羅 , 細谷圭汰 , 長島有希 , 諸菱環 , 相根義昌
日本分子生物学会年会プログラム・要旨集(Web) 2021
Event date： 2021
Language：Japanese Presentation type：Oral presentation (general)
-
日本酒の美味しさ評価における人の官能評価と各種センサー解析との相関性について~同一蔵元製品への応用例~ International conference
南雲和佳子, 相根義昌, 池濱清治, 吉田浩一, 遠藤明仁, 山崎雅夫, 渡部俊弘, 佐藤広顕
日本食品保蔵科学会大会講演要旨集 2021
Event date： 2021
Language：English Presentation type：Oral presentation (general)
-
新品種やまのいも「きたねばり」の褐変要因酵素の性状解析 International conference
吉川宗寿, 田縁勝洋, 平井剛, 宮下慎一郎, 南和広, 相根義昌, 中澤洋三
日本食品保蔵科学会大会講演要旨集 2021
Event date： 2021
Language：English Presentation type：Oral presentation (general)
Teaching Experience 【 display / non-display 】
-
生物化学実験
-
栄養学
-
生物化学（二）
-
生物化学（一）
-
微生物学
Committee Memberships 【 display / non-display 】
-
日本細菌学会 北海道支部幹事
2012
Committee type：Academic society