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真田 篤史 (サナダ アツシ) SANADA Atsushi 教授 |
学内職務経歴 【 表示 ／ 非表示 】
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東京農業大学 国際食料情報学部 国際農業開発学科 教授
2025年04月 - 現在
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東京農業大学 国際食料情報学部 国際農業開発学科 助教
2009年04月 - 2021年03月
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東京農業大学 国際食料情報学部 国際農業開発学科 准教授
2021年04月 - 2025年03月
論文 【 表示 ／ 非表示 】
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Atsushi Sanada and Shinsuke Agehara
plants 12 2023年01月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
Tryptophan is a precursor of indole-3-acetic acid (IAA), which is the major auxin involved in the regulation of lateral root formation. In this study, we used a scanner-based rhizotron system to examine root growth and morphological responses of soybean (Glycine max, ‘Golden Harvest’) seedlings to exogenous tryptophan. Seeds were sown directly in the rhizotron filled with field soil. Tryptophan was applied at 1.9 and 3.8 mg plant−1 by soil drenching or foliar spray. Canopy and root projected area were monitored by analyzing canopy and rhizotron images using ImageJ software. Seedlings were sampled at the first trifoliate stage, 18 days after sowing (DAS), and root morphology was determined by analyzing washed root images using WinRHIZO software. According to contrast analysis, when all tryptophan treatments were pooled, tryptophan application increased canopy and root projected area by 13% to 14% compared with the control at 18 DAS. Tryptophan application also increased root dry matter accumulation by 26%, root:shoot ratio by 24%, and secondary root number by 13%. Tryptophan applied by soil drenching also increased root length and surface area of fine roots (<0.2 mm diameter) by 25% and 21%, respectively, whereas it slightly inhibited primary root elongation. The efficacy of tryptophan soil drenching in stimulating root formation became greater with increasing the application rate. These results suggest that exogenous tryptophan induces auxin-like activities in root development. Soil drenching of tryptophan appears to be an effective strategy in improving the establishment of soybean. Importantly, this strategy is easily implementable by commercial growers with no negative side effect.
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根箱とImageJを用いた迅速かつ安価なダイズ （Glycine max） の根および地上部の生育評価手法の確立 査読あり 国際共著
揚原 晋輔, 真田 篤史
根の研究 29 ( 1 ) 5 - 19 2020年03月
担当区分：最終著者, 責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：根研究学会
<p>著者らは，根箱のスキャン画像解析に基づいた，非破壊によるダイズ （<i>Glycine max</i>） の根の解析手法を確立した．使用した根箱は，木枠とアクリル板で構成された．本研究の主な目的は，初期生育時における野菜苗の根および地上部の発達を，根箱と，フリーソフトである画像解析ソフトImageJを用いて，迅速かつ安価に評価する手法を確立することであった．ダイズは，根箱に播種後，根とキャノピー画像を経時的に調査し，18日目に抜き取った．根の投影面積は，ImageJと2つの市販ソフト （栽培中の画像はWinRHIZO Tron，抜き取り後の画像はWinRHIZO） で測定された．栽培中にImageJとWinRHIZO Tronで測定した根の投影面積は，単回帰分析の結果，決定係数が0.566～0.751の間を示し，有意な相関関係が認められた．また，ImageJと卓上葉面積計で測定した葉面積は，決定係数が0.678であった．したがって，ImageJの測定値は，栽培中の根の投影面積と葉面積の発達を相対評価する指標として利用しうると考えられた．さらに，WinRHIZO Tronは1つの画像解析に約60分要することがあり，ImageJ （1つの画像解析に対し2～3分） と比べて測定項目は多岐にわたるが多大な時間と労力を要した．抜き取り後にImageJとWinRHIZOで測定した根の投影面積は，単回帰分析の結果，高い決定係数 （0.919） を示し，ImageJでも抜き取り後の根の評価が可能であることを示した．したがって，本研究で用いた根箱とImageJによる画像解析は，ダイズの根および地上部の発達を非破壊的に迅速かつ安価に評価し得ることが示された．</p>
DOI： 10.3117/rootres.29.5
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Enhancing salinity tolerance in tomatoes at the reproductive stage by increasing pollen viability 査読あり 国際誌
Nasratullah Habibi, Mohammad Yosuf Fakoor, Shah Mahomoud Faqiri, Zarir Sharaf, Mohammad Sadiq Hotak, Nelofar Danishyar, Mohammad Mustafa Haris, Khuwaja Safiullah Osmani, Takashi Shinohara, Naoki Terada, Atsushi Sanada, and Kaihei Koshio
Bionatura 8 ( １ ) 2023年03月
記述言語：英語 掲載種別：研究論文（学術雑誌）
This study was conducted to mitigate the adverse effects of sodium chloride stress on the reproductive parameters of tomatoes. This experiment was conducted in the greenhouse of the laboratory of tropical horticultural science, department of International agricultural development, Tokyo University of Agriculture. The design was a factorial CRD (completely randomized design) with five sodium chloride (0 mM, 50 mM, 100 mM, 150 mM, and 200 mM) treatments and four primings (0 MPa, 0.4 MPa, 0.8 MPa, and 1.2 MPa) treatments. Micro-Tom seeds were soaked in polyethylene glycol (PEG6000). Salinity was applied through irrigation water when the first flower bloomed. Reproductive-related parameters such as the number of flowers per plant, pollen viability, pollen germination, pollen tube length, number of fruits per plant, fruits size and yield per plant were measured. It was observed that salinity affected the tomato plants severely during the flowering stage, and many flowers did not bear fruit due to the decrease in pollen viability. In addition, electrolyte leakage increased under salt stress, while priming decreased this parameter. Priming improved the number of flowers, pollen viability, and fruits per plant. The best priming treatments were 0.8 MPa and 1.2 MPa for promoting and enhancing tolerance in the reproductive stage.
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Naoki Terada, Kalara Dissanayake, Chiharu Okada, Atsushi Sanada and Kaihei Koshio
horticulturae ９ ( 367 ) 2023年03月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Fertilization is essential for the optimal growth and development of crops; however, the amount of fertilizer can cause positive or negative effects depending on its rate. In addition, the culti-vation system plays a significant role in determining vegetative growth and fruit quality. Therefore, the objectives of this study were to examine the Micro-Tom response to different fertilization rates (first experiment), and to assess the effect of three different cultivation systems on its growth, yield, and fruit quality (second experiment). The fertilization rates used were (A) no fertilizer application control, (B) 0.026 g L−1, (C) 0.052 g L−1, (D) 0.13 g L−1, (E) 0.26 g L−1 standard, (F) 0.52 g L−1, and (G) 1.3 g L−1, and the cultivation systems were conventional, organic, and hydroponic. The results of the first experiment showed that plant growth and yield of Micro-Tom were highly influenced by the fertilization rate and Micro-Tom recorded the highest yield at 0.52 g L−1. In terms of the second experiment, the hydroponically grown Micro-Tom tended to accumulate amino acid, while organic and conventional systems showed more accumulation of sugars and organic acid; the highest yield was recorded in the hydroponic system. The yield obtained in the hydroponic systems was more than double that of the soil cultivation methods. The findings of this study can contribute to promoting the organic and hydroponic cultivation of tomatoes.
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Influence of the Hypobaric Method in Physicochemical Fruit Quality Traits of Yellow and Purple Passion Fruit Stored in Cold Temperature 査読あり
Viera William, Shinohara Takashi, Sanada Atsushi, Terada Naoki, Koshio Kaihei
The Horticulture Journal advpub ( 0 ) 2023年
記述言語：英語 出版者・発行元：The Japanese Society for Horticultural Science
<p>Passion fruit (<i>Passiflora edulis</i>) is a tropical fruit that can be consumed fresh or processed. It is a typical respiratory climacteric fruit which is highly perishable because of the loss of water that causes dehydration and thus shrinkage, affecting the fruit appearance; in addition, some quality traits such as fruit weight, firmness and vitamin C content can also be influenced. Therefore, this fruit has a short shelf life. Storage in low temperatures has been assessed to extent the passion fruit shelf life remaining its fruit quality traits; however, information about the application of the hypobaric method in this fruit is null. The objective of this research was to evaluate the physical and chemical characteristics of the yellow and purple passion fruit under hypobaric storage conditions in low temperature. Results showed the hypobaric method had a positive effect in decreasing fruit weight loss, declining the loss of firmness, and reducing the degradation of vitamin C during the storage period. It decreased the evolution of ethylene which is positive to delay fruit senescence, and the production of CO<sub>2</sub>. Finally, it was the only method which avoids the shrinkage completely in the purple passion fruits (‘Gulupa’, ‘Summer Queen’, and ‘Ruby Star’) and showed minor shrinkage in ‘POR1’ (yellow passion fruit). This method is considered as a promising technique to improve fruit storage.</p>
DOI： 10.2503/hortj.qh-081
書籍等出版物 【 表示 ／ 非表示 】
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沙漠学事典
日本沙漠学会編（ 担当： 共著）
丸善出版 2020年08月
総ページ数：534 担当ページ：302-303 記述言語：日本語 著書種別：事典・辞書
乾燥地域で栽培される作物の特徴について解説した。
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国際農業開発入門-環境と調和した食料増産をめざして-
（ 担当： 共著）
筑波書房 2017年04月
総ページ数：293 担当ページ：85-101 記述言語：日本語 著書種別：教科書・概説・概論
途上国の農業の現場で利用されている組織培養の事例について説明し、組織培養が果たす役割について解説した。