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客員教授 |
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大塚 蔵嵩 (オオツカ クラタカ) - 客員教授 |
論文 【 表示 ／ 非表示 】
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Connecting the dots in the associations between diet, obesity, cancer, and microRNAs 査読あり
Otsuka K, Nishiyama H, Kuriki D, Kawada N, Ochiya T.
SEMINARS IN CANCER BIOLOGY 2023年08月
担当区分：筆頭著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
The prevalence of obesity has reached pandemic levels worldwide, leading to a lower quality of life and higher health costs. Obesity is a major risk factor for noncommunicable diseases, including cancer, although obesity is one of the major preventable causes of cancer. Lifestyle factors, such as dietary quality and patterns, are also closely related to the onset and development of obesity and cancer. However, the mechanisms underlying the complex association between diet, obesity, and cancer remain unclear. In the past few decades, microRNAs (miRNAs), a class of small non-coding RNAs, have been demonstrated to play critical roles in biological processes such as cell differentiation, proliferation, and metabolism, highlighting their importance in disease development and suppression and as therapeutic targets. miRNA expression levels can be modulated by diet and are involved in cancer and obesity-related diseases. Circulating miRNAs can also mediate cell-to-cell communications. These multiple aspects of miRNAs present challenges in understanding and integrating their mechanism of action. Here, we introduce a general consideration of the associations between diet, obesity, and cancer and review the current knowledge of the molecular functions of miRNA in each context. A comprehensive understanding of the interplay between diet, obesity, and cancer could be valuable for the development of effective preventive and therapeutic strategies in future.
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Metastatic prostate cancer-derived extracellular vesicles facilitate osteoclastogenesis by transferring the CDCP1 protein 査読あり 国際共著
Urabe F, Kosaka N, Yamamoto Y, Ito K, Otsuka K, Soekmadji C, Egawa S, Kimura T, Ochiya T.
JOURNAL OF EXTRACELLULAR VESICLES 2023年03月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Bone metastases are still incurable and result in the development of clinical complications and decreased survival for prostate cancer patients. Recently, a number of studies have shown that extracellular vesicles (EVs) play important roles in tumour progression. Here, we show that EVs from metastatic prostate cancer cells promote osteoclast formation in the presence of receptor activator of NF-kappa B ligand (RANKL). EV characterization followed by functional siRNA screening identified CUB-domain containing protein 1 (CDCP1), a transmembrane protein, as an inducer of osteoclastogenesis. Additionally, CDCP1 expression on plasma-derived EVs was upregulated in bone metastatic prostate cancer patients. Our findings elucidate the effect of EVs from metastatic prostate cancer cells on osteoclast formation, which is promoted by CDCP1 located on EVs. Furthermore, our data suggested that CDCP1 expression on EVs might be useful to detect bone metastasis of prostate cancer.
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Possible connection between diet and microRNA in cancer scenario 査読あり 国際共著
Otsuka K, Ochiya T.
SEMINARS IN CANCER BIOLOGY 2021年08月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Early diagnosis, risk prediction, and prevention are critical to reduce disease burden and increase the quality of life. MicroRNAs (miRNAs), a class of small non-coding RNAs, are related to many diseases including cancers. MiRNA dysregulation has been observed in patients as compared to healthy subjects; moreover, miRNA expression profiles can be used to classify poorly differentiated tumors. Recent studies have suggested that early detection of cancers could be achieved by analyzing serum miRNAs and that miRNA profiling would serve to indicate health status. For disease prevention, prophylactic actions should then be taken during early stages before the appearance of symptoms. Notably, it has been shown that naturally occurring compounds can favorably control tumor-related miRNAs, suggesting that food-derived compounds could modulate the expression levels of miRNAs and help recover and maintain the healthy state prior to disease onset. Here we discuss the possible roles of miRNAs as biomarkers and as preventive tools in disease and present an overview of current knowledge regarding the relationship between diet, health, and miRNAs.
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Temperature-dependent fasciation mutants provide a link between mitochondrial RNA processing and lateral root morphogenesis 査読あり
Otsuka K, Mamiya A, Konishi M, Nozaki M, Kinoshita A, Tamaki H, Arita M, Saito M, Yamamoto K, Hachiya T, Noguchi K, Ueda T, Yagi Y, Kobayashi T, Nakamura T, Sato Y, Hirayama T, Sugiyama M.
ELIFE 2021年01月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
Although mechanisms that activate organogenesis in plants are well established, much less is known about the subsequent fine-tuning of cell proliferation, which is crucial for creating properly structured and sized organs. Here we show, through analysis of temperature-dependent fasciation (TDF) mutants of Arabidopsis, root redifferentiation defective 1 (rrd1), rrd2, and root initiation defective 4 (rid4), that mitochondrial RNA processing is required for limiting cell division during early lateral root (LR) organogenesis. These mutants formed abnormally broadened (i.e. fasciated) LRs under high-temperature conditions due to extra cell division. All TDF proteins localized to mitochondria, where they were found to participate in RNA processing: RRD1 in mRNA deadenylation, and RRD2 and RID4 in mRNA editing. Further analysis suggested that LR fasciation in the TDF mutants is triggered by reactive oxygen species generation caused by defective mitochondrial respiration. Our findings provide novel clues for the physiological significance of mitochondrial activities in plant organogenesis.
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Uncovering temperature-dependent extracellular vesicle secretion in breast cancer 査読あり
Otsuka K, Yamamoto Y, Ochiya T.
JOURNAL OF EXTRACELLULAR VESICLES 2020年12月
担当区分：筆頭著者, 責任著者 記述言語：英語
In all living things, temperature is a key factor to maintain function and survive. Animals and plants need to adapt temperature change with optimizing their behaviour and growth by sensing temperature. Similarly, tumour cells must adapt continuously to fluctuations in external conditions including temperature. To find a better environment, cancer cells promote growth and metastasis, which contributes to tumour malignancy. Pathological studies in breast cancer have implied that temperature is associated with disease progression. However, no clear mechanisms have emerged for how thermal changes affect tumour cells and their gene regulation in tumour development and malignancy. Here we discovered the temperature-dependent extracellular vesicle (EV) secretion in breast cancer. Cancer cell growth and EV secretion increased in a temperature-dependent manner, which indicated that temperatures were associated with poor prognosis in breast cancer patients. We also found that low-density lipoprotein receptor (LDLR), a responsible gene for temperature-dependent EV secretion, was upregulated with the increase in temperature. Consistent with our results, LDLR gene has been characterized and identified as a key factor for malignancy in a wide range of cancers. Our findings shed new light on tumour aggressiveness and therapeutic strategies for breast cancer, especially regarding EV formation and secretion, thus providing a new relationship between cancer and EV biology in the light of temperature.
書籍等出版物 【 表示 ／ 非表示 】
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"食品由来エクソソーム様粒子の産業応用の可能性と課題 ”, 「決定版エクソソーム実験ガイド〜世界に通用するプロトコールで高精度なデータを得る!（実験医学別冊 最強のステップUPシリーズ） 」
Otsuka K.（ 担当： 共著）
羊土社 2020年10月
記述言語：日本語 著書種別：学術書
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"植物の視点から見るエクソソーム”, 「エクソソーム―生命現象の鍵を握る膜小胞―」
（ 担当： 共著）
化学同人 2018年09月
記述言語：日本語 著書種別：学術書
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“植物の視点から考えるマイクロRNA/エクソソーム”「パラダイムシフトをもたらすエクソソーム機能研究最前線」
（ 担当： 共著）
NTS出版 2017年03月
記述言語：日本語 著書種別：学術書
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食品成分がマイクロRNA/エクソソームに与える影響 ―新規機能性食品開発の可能性と展望―”、「月刊バイオインダストリー」
シーエムシー出版 2015年11月
記述言語：日本語 著書種別：学術書