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准教授 |
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松谷 峰之介 (マツタニ ミネノスケ) Minenosuke Matsutani 准教授 |
学内職務経歴 【 表示 ／ 非表示 】
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東京農業大学 生物資源ゲノム解析センター 准教授
2023年04月 - 2024年03月
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東京農業大学 生物産業学部 食香粧化学科 准教授
2024年04月 - 現在
論文 【 表示 ／ 非表示 】
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Glucose controls manganese homeostasis through transcription factors regulating known and newly-identified manganese transporter genes in Bacillus subtilis. 国際誌
Mitsuo Ogura, Minenosuke Matsutani, Kei Asai, Michio Suzuki
The Journal of biological chemistry 105069 - 105069 2023年07月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Mn2+ is an essential nutrient whose concentration is tightly controlled in bacteria. In B. subtilis, the Mn2+-activated transcription factor MntR controls Mn2+ transporter genes. However, factors regulating intracellular Mn2+ concentration are incompletely understood. Here, we found that glucose addition induces an increase in intracellular Mn2+ concentration. We determined this upshift was mediated by glucose induction (GI) of the major Mn2+ importer gene mntH by the transcription factor AhrC, which is known to be involved in arginine metabolism and to be indirectly induced by glucose. In addition, we identified novel AhrC-regulated genes encoding the Mn2+ importer YcsG and the ABC-type exporter YknUV. We found the expression of these genes was also regulated by glucose and contributes to the GI of Mn2+ concentrations. ycsG expression is regulated by MntR as well. Furthermore, we analyzed the interaction of AhrC and MntR with the promoter driving ycsG expression and examined the Mn2+-dependent induction of this promoter to identify the transcription factors responsible for the Mn2+ induction. RNA-Seq revealed that disruption of ahrC and mntR affected the expression of 502 and 478 genes, respectively (FDR, <0.001, log2[FC], 2). The AhrC- and/or MntR-dependent expression of twenty promoters was confirmed by LacZ analysis, and AhrC or MntR binding to some of these promoters was observed via electromobility shift assay. The finding that glucose promotes an increase in intracellular Mn2+ levels without changes in extracellular Mn2+ concentrations is reasonable for the bacterium, as intracellular Mn2+ is required for enzymes and pathways mediating glucose metabolism.
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GENETIC VARIATION OF LEPTOTROMBIDIUM (ACARI: TROMBICULIDAE) MITES CARRYING ORIENTIA TSUTSUGAMUSHI, THE BACTERIAL PATHOGEN CAUSING SCRUB TYPHUS. 国際誌
Motohiko Ogawa, Nobuhiro Takada, Shinichi Noda, Mamoru Takahashi, Minenosuke Matsutani, Daisuke Kageyama, Hideki Ebihara
The Journal of parasitology 109 ( 4 ) 340 - 348 2023年07月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Leptotrombidium (Acari: Trombiculidae) mites are carriers of Orientia tsutsugamushi, the bacterial pathogen causing scrub typhus in humans. Classification of Leptotrombidium is vital because limited mite species carry O. tsutsugamushi. Generally, Leptotrombidium at the larval stage (approximately 0.2 mm in size) are used for morphological identification. However, morphological identification is often challenging because it requires considerable skills and taxonomic expertise. In this study, we found that the full-length sequences of the mitochondrial cytochrome c oxidase subunit 1 gene varied among the significant Leptotrombidium. On the basis of these, we modified the canonical deoxyribonucleic acid (DNA) barcoding method for animals by redesigning the primer set to be suitable for Leptotrombidium. Polymerase chain reaction with the redesigned primer set drastically increased the detection sensitivity, especially against Leptotrombidium scutellare (approximately 17% increase), one of the significant mites carrying O. tsutsugamushi. Phylogenetic analysis showed that the samples morphologically classified as L. scutellare and Leptotrombidium pallidum were further split into 3 and 2 distinct subclusters respectively. The mean genetic distance (p-distance) between L. scutellare and L. pallidum was 0.2147, whereas the mean distances within each species were 0.052 and 0.044, respectively. Within L. scutellare, the mean genetic distances between the 3 subclusters were 0.1626-0.1732, whereas the distances within each subcluster were 0.003-0.017. Within L. pallidum, the mean genetic distance between the 2 subclusters was 0.1029, whereas the distances within each subcluster were 0.010-0.013. The DNA barcoding uncovered a broad genetic diversity of Leptotrombidium, especially of L. scutellare and L. pallidum, the notable species carrying O. tsutsugamushi. We conclude that the DNA barcoding using our primers enables precise and detailed classification of Leptotrombidium and implies the existence of a subgenotype in Leptotrombidium that had not been found by morphological identification.
DOI： 10.1645/22-97
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Stepwise metabolic engineering of Corynebacterium glutamicum for the production of phenylalanine.
Naoya Kataoka, Minenosuke Matsutani, Kazunobu Matsushita, Toshiharu Yakushi
The Journal of general and applied microbiology 69 ( 1 ) 11 - 23 2023年06月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Corynebacterium glutamicum was metabolically engineered to produce phenylalanine, a valuable aromatic amino acid that can be used as a raw material in the food and pharmaceutical industries. First, a starting phenylalanine-producer was constructed by overexpressing tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and phenylalanine- and tyrosine-insensitive bifunctional enzyme chorismate mutase prephenate dehydratase from Escherichia coli, followed by the inactivation of enzymes responsible for the formation of dihydroxyacetone and the consumption of shikimate pathway-related compounds. Second, redirection of the carbon flow from tyrosine to phenylalanine was attempted by deleting of the tyrA gene encoding prephenate dehydrogenase, which catalyzes the committed step for tyrosine biosynthesis from prephenate. However, suppressor mutants were generated, and two mutants were isolated and examined for phenylalanine production and genome sequencing. The suppressor mutant harboring an amino acid exchange (L180R) on RNase J, which was experimentally proven to lead to a loss of function of the enzyme, showed significantly enhanced production of phenylalanine. Finally, modifications of phosphoenolpyruvate-pyruvate metabolism were investigated, revealing that the inactivation of either phosphoenolpyruvate carboxylase or pyruvate carboxylase, which are enzymes of the anaplerotic pathway, is an effective means for improving phenylalanine production. The resultant strain, harboring a phosphoenolpyruvate carboxylase deficiency, synthesized 50.7 mM phenylalanine from 444 mM glucose. These results not only provided new insights into the practical mutations in constructing a phenylalanine-producing C. glutamicum but also demonstrated the creation of a potential strain for the biosynthesis of phenylalanine-derived compounds represented by plant secondary metabolites.
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Symbiosis of Carpenter Bees with Uncharacterized Lactic Acid Bacteria Showing NAD Auxotrophy. 国際誌
Shinji Kawasaki, Kaori Ozawa, Tatsunori Mori, Arisa Yamamoto, Midoriko Ito, Moriya Ohkuma, Mitsuo Sakamoto, Minenosuke Matsutani
Microbiology spectrum e0078223 2023年06月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Eusocial bees (such as honey bees and bumble bees) harbor core gut microbiomes that are transmitted through social interaction between nestmates. Carpenter bees are not eusocial; however, recent microbiome analyses found that Xylocopa species harbor distinctive core gut microbiomes. In this study, we analyzed the gut microbiomes of three Xylocopa species in Japan between 2016 and 2021 by V1 to V2 region-based 16S rDNA amplicon sequencing, and 14 candidate novel species were detected based on the full-length 16S rRNA gene sequences. All Xylocopa species harbor core gut microbiomes consisting of primarily lactic acid bacteria (LAB) that were phylogenetically distant from known species. Although they were difficult to cultivate, two LAB species from two different Xylocopa species were isolated by supplementing bacterial culture supernatants. Both genomes exhibited an average LAB genome size with a large set of genes for carbohydrate utilization but lacked genes to synthesize an essential coenzyme NAD, which is unique among known insect symbionts. Our findings of phylogenetically distinct core LAB of NAD auxotrophy reflected the evolution of Xylocopa-restricted bacteria retention and maintenance through vertical transmission of microbes during solitary life. We propose five candidate novel species belonging to the families Lactobacillaceae and Bifidobacteriaceae, including a novel genus, and their potential functions in carbohydrate utilization. IMPORTANCE Recent investigations found unique microbiomes in carpenter bees, but the description of individual microbes, including isolation and genomics, remains largely unknown. Here, we found that the Japanese Xylocopa species also harbor core gut microbiomes. Although most of them were difficult to isolate a pure colony, we successfully isolated several strains. We performed whole-genome sequencing of the isolated candidate novel species and found that the two Lactobacillaceae strains belonging to the Xylocopa-specific novel LAB clade lack the genes for synthesizing NAD, a coenzyme central to metabolism in all living organisms. Here, we propose a novel genus for the two LAB species based on very low 16S rRNA gene sequence similarities and genotypic characters.
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Identification of Capsular Polysaccharide Synthesis Loci Determining Bacteriophage Susceptibility in Tetragenococcus halophilus. 国際誌
Takura Wakinaka, Minenosuke Matsutani, Jun Watanabe, Yoshinobu Mogi, Masafumi Tokuoka, Akihiro Ohnishi
Microbiology spectrum 11 ( 3 ) e0038523 2023年06月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Bacteriophages infecting Tetragenococcus halophilus, a halophilic lactic acid bacterium, have been a major industrial concern due to their detrimental effects on the quality of food products. Previously characterized tetragenococcal phages displayed narrow host ranges, but there is little information on these mechanisms. Here, we revealed the host's determinant factors for phage susceptibility using two virulent phages, phiYA5_2 and phiYG2_4, that infect T. halophilus YA5 and YG2, respectively. Phage-resistant derivatives were obtained from these host strains, and mutations were found at the capsular polysaccharide (CPS) synthesis (cps) loci. Quantification analysis verified that capsular polysaccharide production by the cps derivatives from YG2 was impaired. Transmission electron microscopy observation confirmed the presence of filamentous structures outside the cell walls of YG2 and their absence in the cps derivatives of YG2. Phage adsorption assays revealed that phiYG2_4 adsorbed to YG2 but not its cps derivatives, which suggests that the capsular polysaccharide of YG2 is the specific receptor for phiYG2_4. Interestingly, phiYA5_2 adsorbed and infected cps derivatives of YG2, although neither adsorption to nor infection of the parental strain YG2 by phiYA5_2 was observed. The plaque-surrounding halos formed by phiYA5_2 implied the presence of the virion-associated depolymerase that degrades the capsular polysaccharide of YA5. These results indicated that the capsular polysaccharide is a physical barrier rather than a binding receptor for phiYA5_2 and that phiYA5_2 specifically overcomes the capsular polysaccharide of YA5. Thus, it is suggested that tetragenococcal phages utilize CPSs as binding receptors and/or degrade CPSs to approach host cells. IMPORTANCE T. halophilus is a halophilic lactic acid bacterium that contributes to the fermentation processes for various salted foods. Bacteriophage infections of T. halophilus have been a major industrial problem causing fermentation failures. Here, we identified the cps loci in T. halophilus as genetic determinants of phage susceptibility. The structural diversity of the capsular polysaccharide is responsible for the narrow host ranges of tetragenococcal phages. The information provided here could facilitate future studies on tetragenococcal phages and the development of efficient methods to prevent bacteriophage infections.
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グルコノバクター属酢酸菌CHM43株の2-ケトグルコン酸代謝に関する研究
落合将伍, 片岡尚也, 片岡尚也, 松谷峰之介, 松下一信, 松下一信, 薬師寿治, 薬師寿治
日本農芸化学会中四国支部講演会講演要旨集(Web) 61st 2022年
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サブユニット欠失変異体を用いたグルコノバクター属酢酸菌の膜結合型アルコール脱水素酵素の解析
谷岡和也, PITIWITTAYAKUL Nittaya, 松下一信, 薬師寿治, 片岡尚也, 松谷峰之介
日本農芸化学会中四国支部講演会講演要旨集(Web) 61st 2022年
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高温性プロピオン酸酸化細菌が有する2つのフラボタンパク質の機能的異種発現への取組み
塩田悠介, 松谷峰之介, 山田守, 山田守, 高坂智之, 高坂智之
日本農芸化学会中四国支部講演会講演要旨集(Web) 58th 2021年
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高塩濃度水産発酵物から分離された希少放線菌Amycolatopsis sp.TUA-HKG02Y株の微生物学的特徴
篠田佳那, 松谷峰之介, 長田隆弘, 荒川賢治, 石川森夫, 鈴木敏弘
日本放線菌学会大会講演要旨集 35th (CD-ROM) 2021年
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酢酸菌におけるセルロース生産に関わる遺伝子の欠失と復帰変異
松谷峰之介, THEERAGOOL Gunjana, 薬師寿治, 薬師寿治, 松下一信, 松下一信, 石川森夫
日本生物工学会大会講演要旨集 73rd 2021年
科研費（文科省・学振）獲得実績 【 表示 ／ 非表示 】
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ゲノム情報を利用した共生的プロピオン酸代謝系酵素の成熟化遺伝子の探索と機能解析
研究課題/領域番号：21K05343 2021年04月 - 2024年03月
日本学術振興会 科学研究費助成事業 基盤研究(C) 基盤研究(C)
高坂 智之, 松谷 峰之介
配分額：4030000円 （ 直接経費：3100000円 、 間接経費：930000円 ）