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特命准教授 |
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東京都世田谷区桜丘１−１−１８号館５階 |
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青木 敬太 (アオキ ケイタ) - 特命准教授 |
学外略歴 【 表示 ／ 非表示 】
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東京農業大学 総合研究所 酵母多様性生物学・分類学研究室 特命准教授 博士（生命科学）
2022年04月 - 現在
国名：日本国
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東京農業大学 農生命科学研究所 酵母多様性生物学・分類学研究室 博士研究員
2021年04月 - 現在
国名：日本国
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国立遺伝学研究所 遺伝形質研究系 微生物機能 助教
2019年01月 - 2021年03月
国名：日本国
所属学協会 【 表示 ／ 非表示 】
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日本医真菌学会
2022年06月 - 現在
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酵母遺伝学フォーラム
2010年07月 - 現在
論文 【 表示 ／ 非表示 】
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Whole-genome based phylogeny and comparative genomics of Sporidiobolales and related taxa of Basidiomycetes 査読あり
Yuuki Kobayashi, Naoto Tanaka, Minenosuke Matsutani, Yuuna Kurokawa, Keita Aoki, Moriya Ohkuma, Ri-ichiroh Manabe, Masako Takashima
IMA Fungus 16 ( e141626 ) 2025年05月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Analyses of hyphal diversity in Trichosporonales yeasts based on fluorescent microscopic observations. 査読あり 国際誌
Keita Aoki, Moriya Ohkuma, Takashi Sugita, Yuuki Kobayashi, Naoto Tanaka, Masako Takashima
Microbiology spectrum e0321024 2025年02月
記述言語：英語 掲載種別：研究論文（学術雑誌）
In dimorphic yeasts, hyphal growth is primarily associated with infection and mycosis progression, with Trichosporon asahii causing deep-seated mycosis and summer-type hypersensitivity pneumonitis. Magnesium accelerates hyphal growth in T. asahii, leading to multi-septation, vacuolar expansion, and decreased lipid droplet size. However, the commonality of these phenotypes has not been studied in Trichosporonales yeasts. Therefore, to explore whether similar magnesium-induced phenotypes occur across Trichosporonales yeasts, we examined hyphal growth, multi-septation, vacuolar extension, and lipid droplet size and number in 30 species. Cell length increased with magnesium treatment in 13 yeasts: 5 Trichosporon (T. asahii, Trichosporon aquatile, Trichosporon asteroides, Trichosporon coremiiforme, and Trichosporon ovoides), three Apiotrichum (Apiotrichum brassicae, Apiotrichum montevideense, and Apiotrichum veenhuisii), three Cutaneotrichosporon (Cutaneotrichosporon cavernicola, Cutaneotrichosporon cutaneum, and Cutaneotrichosporon dermatis), Pascua guehoae, and Takashimella koratensis. C. dermatis also underwent pseudo-hyphal growth. Multi-septation increased in seven dimorphic yeasts, including five Trichosporon spp., Trichosporon faecale, and C. dermatis. The vacuolar area was significantly extended in T. asahii, T. aquatile, T. ovoides, and C. dermatis. Lipid droplet size increased only in Trichosporon inkin; however, it decreased in T. asahii, T. coremiiforme, and T. faecale. Additionally, lipid droplet number was preferentially altered in Apiotrichum and Cutaneotrichosporon. These results suggested that magnesium-induced multi-septation and vacuolar area expansion phenotypically distinguish Trichosporon hyphae from Apiotrichum and Cutaneotrichosporon hyphae and distinguish C. dermatis pseudo-hyphae from Cutaneotrichosporon spp. Moreover, differences in lipid droplet size can discriminate species within Trichosporon. Our results suggest that phenotypic alteration via magnesium treatment can contribute to the characterization of Trichosporonales yeasts. These findings provide insights into fungal pathogenesis and may support new treatment strategies.IMPORTANCEMagnesium sulfate considerably affects hyphal growth and cellular organization in Trichosporon asahii. To examine the commonality of this phenotype in Trichosporonales, we treated 30 Trichosporonales yeasts with magnesium sulfate and observed genus-level phenotypic alterations. Using cell length measurement, lipid droplet staining, septum staining, and vacuole staining, considerable hyphal diversity was observed among Trichosporonales. Notably, differences in the multi-septation phenotype and vacuolar size distinguished Trichosporon hyphae from Apiotrichum and Cutaneotrichosporon hyphae and distinguished Cutaneotrichosporon dermatis from other Cutaneotrichosporon spp. Moreover, differences in lipid droplet phenotype divided Trichosporon hyphae into two groups. Our study revealed the relationship between hyphal morphology and phylogenetic systematics in Trichosporonales.
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Yuuki Kobayashi, Ayane Kayamori, Keita Aoki, Yuh Shiwa, Minenosuke Matsutani, Nobuyuki Fujita, Takashi Sugita, Wataru Iwasaki, Naoto Tanaka, Masako Takashima
BMC Genomics 24 ( 1 ) 2023年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Springer Science and Business Media LLC
Abstract
Background
Since DNA information was first used in taxonomy, barcode sequences such as the internal transcribed spacer (ITS) region have greatly aided fungal identification; however, a barcode sequence alone is often insufficient. Thus, multi-gene- or whole-genome-based methods were developed. We previously isolated Basidiomycota yeasts classified in the Trichosporonales. Some strains were described as Cutaneotrichosporon cavernicola and C. spelunceum, whereas strain HIS471 remained unidentified. We analysed the genomes of these strains to elucidate their taxonomic relationship and genetic diversity.
Results
The long-read-based assembly resulted in chromosome-level draft genomes consisting of seven chromosomes and one mitochondrial genome. The genome of strain HIS471 has more than ten chromosome inversions or translocations compared to the type strain of C. cavernicola despite sharing identical ITS barcode sequences and displaying an average nucleotide identity (ANI) above 93%. Also, the chromosome synteny between C. cavernicola and the related species, C. spelunceum, showed significant rearrangements, whereas the ITS sequence identity exceeds 98.6% and the ANI is approximately 82%. Our results indicate that the relative evolutionary rates of barcode sequences, whole-genome nucleotide sequences, and chromosome synteny in Cutaneotrichosporon significantly differ from those in the model yeast Saccharomyces.
Conclusions
Our results revealed that the relative evolutionary rates of nucleotide sequences and chromosome synteny are different among fungal clades, likely because different clades have diverse mutation/repair rates and distinct selection pressures on their genomic sequences and syntenic structures. Because diverse syntenic structures can be a barrier to meiotic recombination and may lead to speciation, the non-linear relationships between nucleotide and synteny diversification indicate that sequence-level distances at the barcode or whole-genome level are not sufficient for delineating species boundaries.DOI： 10.1186/s12864-023-09718-2
その他リンク： https://link.springer.com/article/10.1186/s12864-023-09718-2/fulltext.html
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Hyphal Growth in <i>Trichosporon asahii</i> Is Accelerated by the Addition of Magnesium 査読あり
Keita Aoki, Kosuke Yamamoto, Moriya Ohkuma, Takashi Sugita, Naoto Tanaka, Masako Takashima
Microbiology Spectrum 11 ( 3 ) 2023年06月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：American Society for Microbiology
Understanding the mechanism underlying fungal dimorphism is crucial to discern its invasion into human cells. Invasion is caused by the hyphal form rather than the yeast form; therefore, it is important to understand the mechanism of transition from the yeast to hyphal form.
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A genome-informed higher rank classification of the biotechnologically important fungal subphylum Saccharomycotina 査読あり 国際共著
M. Groenewald, C.T. Hittinger, K. Bensch, D.A. Opulente, X.-X. Shen, Y. Li, C. Liu, A.L. LaBella, X. Zhou, S. Limtong, S. Jindamorakot, P. Gonçalves, V. Robert, K.H. Wolfe, C.A. Rosa, T. Boekhout, N. Čadež, G. Péter, J.P. Sampaio, M.-A. Lachance, A.M. Yurkov, H.-M. Daniel, M. Takashima, K. Boundy-Mills, D. Libkind, K. Aoki, T. Sugita, A. Rokas
Studies in Mycology 105 1 - 22 2023年06月
書籍等出版物 【 表示 ／ 非表示 】
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国際菌学会（IMC12）および国際酵母学会（ICY16）で開催されたThe Yeasts, A Taxonomic Studyの更新版発行に関するミーティング
青木敬太, 小林裕樹, 高島昌子（ 担当： 共著 , 範囲： 40 (2): 1-4）
日本微生物資源学会誌 2024年
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微生物資源の整備と利活用の戦略
青木敬太, 小林裕樹, 高島昌子（ 担当： 分担執筆 , 範囲： 第４章第２節 真菌の命名と系統分類に関する課題）
エヌ・ティー・エス 2023年09月 （ ISBN:9784860438586 ）
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染色体サイクル ゲノム恒常性維持、継承とダイナイクス内の概論「染色体の分配とM期制御」
青木敬太、仁木宏典（ 担当： 共著）
共立出版 2009年
記述言語：日本語 著書種別：学術書
MISC 【 表示 ／ 非表示 】
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全ゲノム情報に基づいた担子菌酵母の変異と種分化の解析
小林裕樹, 栢森綺音, 青木敬太, 志波優, 松谷峰之介, 藤田信之, 杉田隆, 岩崎渉, 大熊盛也, 田中尚人, 高島昌子
日本分子生物学会年会プログラム・要旨集(Web) 46th 2023年
科研費（文科省・学振）獲得実績 【 表示 ／ 非表示 】
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新規モデル生物を用いた核膜動態の研究
2013年04月 - 2014年03月
科学研究費補助金 上原記念生命科学財団研究奨励金
青木敬太
講演・口頭発表等 【 表示 ／ 非表示 】
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担子菌酵母Trichosporon asahiiのニ形性に関与する遺伝子機能の研究
青木敬太, 松谷峰之介, 大熊盛也, 杉田隆, 小林裕樹, 田中尚人, 高島昌子
第47回日本分子生物学会年会 2024年11月
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Dimorphic transition between yeast, hypha, and arthroconidia morphologies by Trichosporon asahii 招待あり
Keita Aoki, Minenosuke Matsutani, Moriya Ohkuma, Takashi Sugita, Yuuki Kobayashi, Naoto Tanaka, Masako Takashima
The 8th Congress of Asia-Pacific Society for Medical Mycology (APSMM 2024) 2024年11月
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蛍光顕微鏡観察によって明らかになるトリコスポロン酵母の性状 招待あり
青木敬太, 大熊盛也, 杉田隆, 小林裕樹, 田中尚人, 高島昌子
第68回日本医真菌学会総会・学術集会 2024年11月
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Transition from hyphae to arthroconidia requires a nucleolar protein NOP14 ortholog in Trichosporon asahii
Keita Aoki, Minenosuke Matsutani, Moriya Ohkuma, Takashi Sugita, Yuuki Kobayashi, Naoto Tanaka, Masako Takashima
16th International Congress on Yeasts 2024年10月
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蛍光顕微鏡観察に基づいたトリコスポロン酵母の種間多様性解析
青木敬太, 大熊盛也, 杉田隆, 小林裕樹, 田中尚人, 高島昌子
日本微生物資源学会第30回 2024年07月