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教授 |
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研究室住所 |
神奈川県厚木市船子1737 東京農大デザイン農学科 |
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外部リンク |
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加藤 浩 (カトウ ヒロシ) - 教授 |
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麦あと晩植栽培に適した早生・良食味の水稲新品種「ほしみのり」の育成
黒木 慎, 山口 誠之, 石井 卓朗, 小林 伸哉, 平林 秀介, 竹内 善信, 後藤 明俊, 春原 嘉弘, 加藤 浩, 佐藤 宏之, 常松 浩史, 安東 郁男, 根本 博, 井辺 時雄, 太田 久稔, 前田 英郎, 出田 収, 平山 正賢, 池ヶ谷 智仁, 津田 直人, 田中 淳一
農研機構研究報告 = Journal of the NARO Research and Development ( 6 ) 21 - 29 2021年03月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：国立研究開発法人 農業・食品産業技術総合研究機構
「ほしみのり」は，良食味，縞葉枯病抵抗性の「関東209 号（さとじまん）」と良質，良食味の「越南171 号」の交雑後代より育成された，早生，良食味品種である．育成地（農研機構谷和原水田圃場（茨城県つくばみらい市））における「ほしみのり」の特徴は以下の通りである．出穂期，成熟期はともに"やや早" に属し，「朝の光」よりやや早い．「朝の光」と比較して，稈長，穂長は長く，穂数は少ない．草型は"穂重型" である．収量は，「朝の光」に対して，晩植・標肥区で19%，早植・標肥区で8% 多い．炊飯米の食味は「コシヒカリ」並の良食味である．玄米の外観品質は"中の上"，高温登熟性は"やや弱" である．いもち病真性抵抗性遺伝子Pia を持つと推定され，圃場抵抗性は，葉いもちが"中"，穂いもちが"やや弱" である．縞葉枯病抵抗性遺伝子Stvb-i を持ち，縞葉枯病には"抵抗性" で，白葉枯病抵抗性は"中"である．穂発芽性は "難" である．以上の特性から，「ほしみのり」は，北関東を中心とした麦あと晩植地帯向けの品種として，普及・活用が期待される．
DOI： 10.24514/00006620
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Feng Li, Akira Komatsu, Miki Ohtake, Heesoo Eun, Akemi Shimizu, Hiroshi Kato
Scientific Reports 10 ( 1 ) 2020年09月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Springer Science and Business Media LLC
<title>Abstract</title>
Loss of seed shattering has been regarded as a key step during crop domestication. Mutagenesis contributes to the development of novel crop cultivars with a desired seed-shattering habit in a relatively short period of time, but also to uncovering the genetic architecture of seed shattering. ‘Minamiyutaka’, a non-shattering <italic>indica</italic> rice cultivar, was developed from the easy-shattering cultivar ‘Moretsu’ by mutation breeding via gamma-ray irradiation. In present study, we observed significant differences in shattering habit, breaking tensile strength, and abscission zone structure between ‘Moretsu’ and ‘Minamiyutaka’. Whole-genome mutation analysis of ‘Minamiyutaka’ newly identified a 13-bp deletion causing defective splicing in exon 3 of the <italic>OsSh1</italic> gene which has previously been referred to as a candidate for controlling seed shattering. Using CRISPR/Cas9 gene editing, we demonstrated that loss-of-function mutation in <italic>OsSh1</italic> causes non-shattering in rice. Furthermore, gene expression analysis suggests that <italic>OsSh1</italic> may function downstream of <italic>qSH1</italic>, a known key gene involved in abscission zone differentiation<italic>.</italic> Nucleotide diversity analysis of <italic>OsSh1</italic> in wild rice accessions and cultivars revealed that <italic>OsSh1</italic> has been under strong selection during rice domestication, and a missense mutation might have contributed to the reduction of seed shattering from the wild progenitors to cultivated rice. -
The Selection of Gamma-Ray Irradiated Higher Yield Rice Mutants by Directed Evolution Method 査読あり
Hiroshi Kato, Feng Li, Akemi Shimizu
Plants 9 ( 8 ) 1004 - 1004 2020年08月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：MDPI AG
We have succeeded in selecting four higher yield mutants from five gamma-ray irradiated high-yielding Japanese rice varieties using a novel approach. A total of 464 M2 plants which had heavier total panicle weights per plant were first selected from 9801 irradiated M2 plants. Their higher yields were confirmed by yield trials conducted for three years with a six to ten-pairwise replicated plot design. FukuhibikiH6 and FukuhibikiH8 were selected from an irradiated high-yielding variety Fukuhibiki and showed 1.2% to 22.5% higher yield than their original significantly. YamadawaraH3 was selected from an irradiated high-yielding variety Yamadawara and its yield advantages were 2.7% to 3.9%. However, there was no difference in the genotypes of the 96 SNP (single nucleotide polymorphism) markers between the higher yield mutants and their respective original varieties. The differences in the measured phenotypical traits between each mutant and its original variety were not constant and the actual differences were marginal. Therefore, the higher yields of the selected mutants were likely to have been caused by physiological traits rather than phenotypical traits. The selection method used in this study is an application of the directed evolution method which has long been commonly used in the substantial improvements of microorganisms and their proteins.
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Yutaka Oono, Hiroyuki Ichida, Ryouhei Morita, Shigeki Nozawa, Katsuya Satoh, Akemi Shimizu, Tomoko Abe, Hiroshi Kato, Yoshihiro Hase
bioRxiv 2020年06月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cold Spring Harbor Laboratory
<title>Abstract</title>Ion beams are physical mutagens used for plant and microbe breeding that cause mutations via a distinct mechanism from those of chemical mutagens or gamma rays. We utilized whole-exome sequencing of rice DNA in order to understand the properties of ion beam-induced mutations in a genome-wide manner. DNA libraries were constructed from selected carbon-ion-beam-induced rice mutants by capturing with a custom probes covering 66.3 M bases of nearly all exons and miRNAs predicted in the genome. A total of 56 mutations, including 24 single nucleotide variations, 23 deletions, and 5 insertions, were detected in five mutant rice lines (two dwarf and three early-heading-date mutants). The mutations were distributed among all 12 chromosomes, and the average mutation frequency in the M1 generation was estimated to be 2.7 × 10-7 per base. Many single base insertions and deletions were associated with homopolymeric repeats, whereas larger deletions up to seven base pairs were observed at polynucleotide repeats in the DNA sequences of the mutation sites. Of the 56 mutations, six were classified as high-impact mutations that caused a frame shift or loss of exons. A gene that was functionally related to the phenotype of the mutant was disrupted by a high-impact mutation in four of the five lines tested, suggesting that whole-exome sequencing of ion-beam-irradiated mutants could facilitate the detection of candidate genes responsible for the mutant phenotypes.
DOI： 10.1101/666677
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A rice gene that confers broad-spectrum resistance to β-triketone herbicides 査読あり
Hideo Maeda, Kazumasa Murata, Nozomi Sakuma, Satomi Takei, Akihiko Yamazaki, Md. Rezaul Karim, Motoshige Kawata, Sakiko Hirose, Makiko Kawagishi-Kobayashi, Yojiro Taniguchi, Satoru Suzuki, Keisuke Sekino, Masahiro Ohshima, Hiroshi Kato, Hitoshi Yoshida, Yuzuru Tozawa
Science 365 ( 6451 ) 393 - 396 2019年07月
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池田浩輔, 清水克哉, 澤村紀子, 石井卓朗, 小林伸哉, 黒木慎, 加藤浩, 春原嘉弘
出願番号：特願31449(品種登録) 出願日：2016年09月
特許番号/登録番号：特許27550 (Japan Plant Variety Registration) 登録日：2019年08月 発行日：2019年08月
出願国：国内 取得国：国内
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池田浩輔, 清水克哉, 澤村紀子, 石井卓朗, 小林伸哉, 黒木慎, 加藤浩, 春原嘉弘
出願番号：特願31450(品種登録) 出願日：2016年09月
特許番号/登録番号：特許27551 (Japan Plant Variety Registration) 登録日：2019年08月 発行日：2019年08月
出願国：国内 取得国：国内
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太田久稔, 山口誠之, 滝田正, 梶亮太, 福嶌陽, 中込弘二, 片岡知守, 遠藤貴司, 横上晴郁, 加藤浩, 市場茂夫, 辻内啓次郎
出願番号：特願25882(品種登録) 出願日：2011年05月
特許番号/登録番号：特許22530(Japan Plant Variety Registration) 登録日：2013年03月 発行日：2013年03月
出願国：国内 取得国：国内
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竹内善信, 安東郁男, 根本博, 加藤浩, 平林秀介, 太田久稔, 石井卓朗, 前田英郎, 久野陽子, 井邊時雄, 佐藤宏之, 出田収, 平山正賢
出願番号：特願23505(品種登録) 出願日：2009年02月
特許番号/登録番号：特許20745(Japan Plant Variety Registration) 登録日：2011年03月 発行日：2011年03月
出願国：国内 取得国：国内
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山口誠之, 滝田正, 東正昭, 片岡知守, 遠藤貴司, 中込弘二, 横上晴郁, 加藤浩, 田村泰章
出願番号：特願22847(品種登録) 出願日：2008年08月
特許番号/登録番号：特許20532(Japan Plant Variety Registration) 登録日：2011年03月 発行日：2011年03月
出願国：国内 取得国：国内