Updated on 2022/08/05

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MIURA Daiki

Assistant Professor

Title

Assistant Professor

Degree 【 display / non-display

  • 博士(農学) ( 2004.03   東京農工大学 )

Employment Record in Research 【 display / non-display

  • Tokyo University of Agriculture   Faculty of Applied Bio-Science   Department of Bio-Science   Research Assistant

    2006.04 - 2007.03

  • Tokyo University of Agriculture   Faculty of Applied Bio-Science   Department of Bio-Science   Assistant Professor

    2007.04 - 2017.03

  • Tokyo University of Agriculture   Faculty of Life Sciences   Department of Bioscience   Assistant Professor

    2017.04

Professional Memberships 【 display / non-display

  • 日本栄養・食糧学会

    2002.04

  • 日本農芸化学会

    2001.04

Qualification and License 【 display / non-display

  • Hazardous Material Handler (second kind)

Papers 【 display / non-display

  • Autism-associated ANK2 regulates embryonic neurodevelopment Reviewed

    Shotaro Kawano, Masayuki Baba, Hotaka Fukushima, Daiki Miura, Hitoshi Hashimoto, Takanobu Nakazawa

    Biochem Biophys Res Commun   605   45 - 50   2022.05

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1016/j.bbrc.2022.03.058

  • PI3K regulates BMAL1/CLOCK-mediated circadian transcription from the Dbp promoter. Reviewed

    Morishita Y, Miura D, Kida S.

    Bioscience, Biotechnology, and Biochemistry   29   1 - 10   2016.03

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    Language:English   Publishing type:Research paper (scientific journal)  

    Dbp遺伝子プロモーターにおけるBMAL1/CLOCKの仲介するサーカディアン転写活性化に対するPI3Kの制御メカニズム解析

  • Roles of CREB in the regulation of FMRP by group I metabotropic glutamate receptors in cingulate cortex Reviewed

    Hansen Wang, Yoshikazu Morishita, Daiki Miura, Jose R Naranjo, Satoshi Kida, and Min Zhuo

    Molecular Brain   5 ( 27 )   e27 - e27   2012.08

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    Language:English   Publishing type:Research paper (scientific journal)  

    ドミナントアクティブなCREB変異体を過剰発現する成体マウスのACCニューロンにおいてmGluRを活性化した場合、FMRP遺伝子発現量が増加した。一方、シナプス可塑性に関係するDREAM遺伝子については、カルシウムイオン非感受性変異体を過剰発現するマウスにおいて、FMRP遺伝子発現量に変化は観察されなかった。したがって、転写調節因子CREBは、mGluRsを介してFMRP遺伝子の転写を制御することが考えられた。

    DOI: 10.1186/1756-6606-5-27

  • Effect of apple polyphenol extract on hepatoma proliferation and invasion in culture and on tumor growth, metastasis, and abnormal lipoprotein profiles in hepatoma-bearing rats. Reviewed

    Daiki Miura, Yutaka Miura, Kazumi Yagasaki

    Bioscience, Biotechnology, and Biochemistry   71 ( 11 )   2743 - 2750   2007.11

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    in vitroとin vivoにおいて、ラット腹水肝癌細胞(AH109A)の増殖と浸潤に対するりんごポリフェノール抽出成分(APE)の抑制効果について解析を行った。その結果、APEを経口投与したラット血清は、AH109Aの増殖と浸潤を抑制した。また担癌モデル動物に対するAPE摂取の効果を解析した結果、癌の増殖・転移に対して抑制傾向を示すことが示唆された。さらに、血清中の(VLDL+LDL)-Chの有意な低下が観察された。

  • Hydroxymatairesinol and its mammalian metabolite enterolactone reduce the growth and metastasis of subcutaneous AH109A hepatomas in rats Reviewed

    Daiki Miura, Niina M. Saarinen, Yutaka Miura, Risto Santti and Kazumi Yagasaki

    Nutrition and cancer   58 ( 1 )   49 - 59   2007.08

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    癌細胞の浸潤促進には、活性酸素種が関与していることが考えられる。そこで、化学構造から抗酸化作用が予想されるリグナン成分(HMR)およびその代謝産物(ENL)の肝癌細胞浸潤抑制機構の解析を行った。その結果、HMRとENLは肝癌細胞内の過酸化レベルを低下させることを明らかにした。また、担癌モデル動物に対するHMRとENL摂取の効果を解析した結果、癌の増殖・転移に対して、抑制傾向を示すことが示唆され、さらに脂質代謝異常の改善効果も観察された。

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Presentations 【 display / non-display

  • Effects of environmental enrichment on mouse brain function and its underlying molecular mechanisms

    2022.03 

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    Event date: 2022.03

    Language:Japanese   Presentation type:Poster presentation  

  • Functional analysis of an autism spectrum disorder-related gene product ANK2 during fetal brain development International conference

    2022.03 

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    Event date: 2022.03

    Language:Japanese   Presentation type:Oral presentation (general)  

  • PI3K regulates BMAL1/CLOCK-dependent circadian transcription rhythms International conference

    Yoshikazu MORISHITA, Daiki MIURA, Satoshi KIDA

    The 38th Annual Meeting of the Molecular Biology Society of Japan  2015.12 

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    Event date: 2015.12

    Language:English   Presentation type:Poster presentation  

    Venue:神戸  

    Circadian transcription rhythms mediated by BMAL1/CLOCK plays essential roles in behavioral and physiological circadian rhythms. Abundant studies have shown that BMAL1/CLOCK-mediated transcription rhythms are generated through positive and negative feedback loops. However, roles and functions of intracellular signal transduction pathways for regulation of BMAL1/CLOCK-mediated circadian transcription rhythms are still unclear. To understand this, we have tried to identify intracellular signal transduction pathways that play regulatory roles in BMAL1/CLOCK-mediated transcription. We found that pharmacological inhibition of PI3K dose-dependently impaired circadian expression pattern of Dbp mRNA, a BMAL1/CLOCK-target genes. Knockdown of PI3K catalytic subunit p110β, but not p110α, significantly decreased Dbp mRNA levels. Moreover, the pharmacological inhibition of PI3K blocked the promoter activity of Dbp gene. ChIP analysis showed that the inhibition of PI3K decreased in the recruitment of BMAL1/CLOCK to E-box of Dbp gene. We also found that the inhibition of PI3K blocked hetero-dimerization of BMAL1 with CLOCK. These results suggest that PI3K regulates binding of BMAL1/CLOCK to E-box elements by targeting dimerization of BMAL1 and CLOCK.

  • PI3K regulates BMAL1/CLOCK-mediated circadian transcription rhythms International conference

    Yoshikazu MORISHITA, Daiki MIURA, Satoshi KIDA

    Biological Oscillators: Design, Mechanism, Function, EMBO | EMBL 2015 Symposia  2015.11 

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    Event date: 2015.11

    Language:English   Presentation type:Poster presentation  

    Venue:EMBL Heidelberg, Germany  

  • PI3K によるBMAL1/CLOCKを介する時計遺伝子群の発現制御

    森下良一、三浦大樹、喜田聡

    題38回日本神経科学大会  2015.07 

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    Event date: 2015.07

    Language:Japanese   Presentation type:Poster presentation  

    Venue:神戸  

    Circadian transcription rhythms mediated by BMAL1/CLOCK play essential roles in behavioral and physiological circadian rhythms. Abundant studies have shown that BMAL1/CLOCK-mediated transcription rhythms are generated through positive and negative feedback loops. However, roles and functions of intracellular signal transduction pathways for regulation of BMAL1/CLOCK-mediated circadian transcription rhythms are still unclear. To understand this, we have tried to identify intracellular signal transduction pathways that play regulatory roles in BMAL1/CLOCK-mediated transcription. We examined effects of various selective inhibitors of intracellular signal transduction pathways on BMAL1/CLOCK-mediated circadian transcription rhythms in NIH3T3 cells. Cells were treated with 50% horse serum for 2hrs (serum shock) to induce circadian transcription rhythms, and then examined expression rhythms of BMAL1/CLOCK-target genes. We found that pharmacological inhibition of phosphoinositide 3-kinase (PI3K) significantly modulated circadian oscillation of mRNA levels for several BMAL1/CLOCK-targeted clock genes. Importantly, the inhibition of PI3K strongly decreased expression levels of Dbp mRNA. Consistently, luciferase reporter assay revealed that the inhibition of PI3K also decreased the activity of Dbp promoter. Moreover, knockdown of PI3K catalytic subunit p110β, but not p110α, using shRNA significantly decreased expression levels of Dbp mRNA. We have further examined mechanisms by which how BMAL1/CLOCK-mediated transcription was modulated by PI3K. Our results using ChIP assay suggested that the inhibition of PI3K blocks binding of BMAL1/CLOCK to E-box sequence. Taken together, we showed that PI3K plays modulatory roles in BMAL1/CLOCK-mediated transcription rhythms.

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