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SASAKI Yasuyuki Professor |
From Graduate School 【 display / non-display 】
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University of Tsukuba Graduate School, Division of Agriculture Doctor Course Completed
2001.04 - 2004.03
Country：Japan
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Life Sciences Department of Bioscience Professor
2024.04
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Tokyo University of Agriculture Faculty of Applied Bio-Science Department of Bio-Science Assistant Professor
2007.04 - 2017.03
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Tokyo University of Agriculture Faculty of Life Sciences Department of Bioscience Assistant Professor
2017.04 - 2017.09
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Tokyo University of Agriculture Faculty of Life Sciences Department of Bioscience Associate Professor
2017.10 - 2024.03
Papers 【 display / non-display 】
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Fenoxycarb, a carbamate insect growth regulator, inhibits brassinosteroid action Reviewed
Ito Shinsaku, Kawada Kojiro, Saeki Yasumasa, Nakano Takeshi, Sasaki Yasuyuki, Yajima Shunsuke
Journal of Pesticide Science advpub ( 0 ) 2023
Language：English Publishing type：Research paper (scientific journal) Publisher：Pesticide Science Society of Japan
<p>Brassinosteroids (BRs) are steroid hormones that regulate plant growth, development, and stress resistance. In this study, we evaluated the effect of agrochemicals on dark-induced hypocotyl elongation, which is regulated by BRs, to identify novel chemicals that regulate BR action. We found that the juvenile hormone agonist fenoxycarb inhibited dark-induced hypocotyl elongation in <i>Arabidopsis</i>. Treatment with the same class of juvenile hormone agonist, pyriproxyfen, did not affect hypocotyl elongation. Co-treatment with fenoxycarb and BR partly canceled the fenoxycarb-induced hypocotyl suppression. In addition, gene expression analysis revealed that fenoxycarb altered the BR-responsive gene expression. These results indicate that fenoxycarb is a BR action inhibitor.</p>
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Role of DevR phosphorylation in nitric oxide homeostasis and signaling of Streptomyces coelicolor A3(2) M145 Reviewed International journal
Sota Honm, Shinsaku Ito, Shunsuke Yajima, Yasuyuki Sasaki
FEMS Microbiol Lett . 2023
Authorship：Corresponding author Language：English
Our previous studies revealed that a two-component system (TCS), DevS, and DevR, regulate both nitric oxide (NO) signaling and NO homeostasis in the actinobacterium Streptomyces coelicolor A3(2) M145, suggesting a reasonable system for NO-dependent metabolism. In this study, sequence alignment of DevR and DevR homologs found Asp66 (D66) and Thr196 (T196) as predicted phosphorylation sites of DevR. Phos-tag gel electrophoretic mobility shift assay suggested that D66 and T196 are involved in the phosphorylation of DevR. The respective point mutations of D66 and T196 significantly decreased the transcriptional activity of DevR, which affected nitrite production and aerial mycelium formation. These results suggested that both D66 and T196 of DevR are important for the regulation of NO homeostasis and signaling in S. coelicolor A3(2) M145.
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Synthesis of Carlactone Derivatives to Develop a Novel Inhibitor of Strigolactone Biosynthesis Reviewed International journal
Kojiro Kawada, Tatsuo Saito, Satoshi Onoda, Takuma Inayama, Ikuo Takahashi, Yoshiya Seto, Takahito Nomura, Yasuyuki Sasaki, Tadao Asami, Shunsuke Yajima, Shinsaku Ito
ACS Omega 8 ( 15 ) 2023
Strigolactones (SLs), phytohormones that inhibit shoot branching in plants, promote the germination of root-parasitic plants, such as Striga spp. and Orobanche spp., which drastically reduces the crop yield. Therefore, reducing SL production via chemical treatment may increase the crop yield. To design specific inhibitors, it is valid to utilize the substrate structure of the target proteins as lead compounds. In this study, we focused on Os900, a rice enzyme that oxidizes the SL precursor carlactone (CL) to 4-deoxyorobanchol (4DO), and synthesized 10 CL derivatives. The effects of the synthesized CL derivatives on SL biosynthesis were evaluated by the Os900 enzyme assay in vitro and by measuring 4DO levels in rice root exudates. We identified some CL derivatives that inhibited SL biosynthesis in vitro and in vivo.
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Insect growth regulators with hydrazide moiety inhibit strigolactone biosynthesis in rice Reviewed
47 ( 1 ) 2022
Language：English Publishing type：Research paper (scientific journal)
本研究では、SLの生合成を阻害する新規リード化合物を同定するために、SLの生合成に対する農薬の影響を評価した。その結果、クロマフェノジドとメトキシフェノジドがSL生合成の新規リード阻害剤であることを示唆した。
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Canonical strigolactones are not the major determinant of tillering but important rhizospheric signals in rice Reviewed
Shinsaku Ito, Justine Braguy, Jian You Wang , Akiyoshi Yoda, Valentina Fiorilli, Ikuo Takahashi, Muhammad Jamil, Abrar Felemban, Sho Miyazaki, Teresa Mazzarella, Guan-Ting Erica Chen, Akihisa Shinozawa, Aparna Balakrishna, Lamis Berqdar, Chakravarty Rajan, Shawkat Ali, Imran Haider, Yasuyuki Sasaki, Shunsuke Yajima, Kohki Akiyama, Luisa Lanfranco, Matias D Zurbriggen, Takahito Nomura, Tadao Asami, Salim Al-Babili
Sci Adv. 8 ( 44 ) 2022
Language：English Publishing type：Research paper (scientific journal)
Strigolactones (SLs) are a plant hormone inhibiting shoot branching/tillering and a rhizospheric, chemical signal that triggers seed germination of the noxious root parasitic plant Striga and mediates symbiosis with beneficial arbuscular mycorrhizal fungi. Identifying specific roles of canonical and noncanonical SLs, the two SL subfamilies, is important for developing Striga-resistant cereals and for engineering plant architecture. Here, we report that rice mutants lacking canonical SLs do not show the shoot phenotypes known for SL-deficient plants, exhibiting only a delay in establishing arbuscular mycorrhizal symbiosis, but release exudates with a significantly decreased Striga seed-germinating activity. Blocking the biosynthesis of canonical SLs by TIS108, a specific enzyme inhibitor, significantly lowered Striga infestation without affecting rice growth. These results indicate that canonical SLs are not the determinant of shoot architecture and pave the way for increasing crop resistance by gene editing or chemical treatment.
Misc 【 display / non-display 】
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微生物を用いた廃水からの窒素除去システム
佐々木康幸、高谷直樹、祥雲弘文
生物工学会誌 83 ( 4 ) 202 - 202 2005.04
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：社団法人 日本生物工学会
現在問題となっている工業、農業排水に含まれる多量の硝酸対窒素が引き起こす、温室効果ガスの大量放出による地球温暖化への影響を問題提起した。その解決法として、独自に単離した微生物を用いた廃水処理システムによる解決法を提案した。
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Nitric oxide synthesis by the Actinomycete S. antibioticus
Yasuyuki Sasaki, Naoki Takaya, Akira Nakamura, Hirohumi, Shoun
NITRIC OXIIDE: Biology and Chemistry ６ ( ４ ) ４５９ - ４６０ 2002.06
Authorship：Lead author Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Elsevier Science
放線菌Streptomyces antibioticusが有機体窒素から亜硝酸塩を培地に蓄積することを見出した。同培養条件において一酸化窒素酸化酵素flavohemoglobinと硝酸塩還元酵素を生産していることから、一酸化窒素合成酵素の由来の一酸化窒素生産能が示唆され、放線菌内で一酸化窒素、硝酸、そして亜硝酸を生産し、エネルギーを得ていることが示唆された。
Presentations 【 display / non-display 】
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The Streptomyces nitrogen oxide cycle and its regulation International conference
Yasuyuki Sasaki
13th International symposium on the genetics of industrial microorganisms 2016.10
Event date： 2016.10
Language：English Presentation type：Oral presentation (invited, special)
Venue：中華人民共和国 武漢
放線菌Streptomyces coelicolorに見出した窒素酸化物生産経路の生理的意義、及び制御機構について発表した。
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Streptomyces coelicolor A3(2)における窒素酸化物によって制御される遺伝子群の解析
小川翔太、草間慎一郎、杉浦遼、田瀬有紀、伊藤晋作、矢嶋俊介、祥雲弘文、佐々木康幸
日本農芸化学会 2014.03
Event date： 2014.03
Language：English Presentation type：Oral presentation (general)
Venue：東京
放線菌内で生産された一酸化窒素により制御される遺伝子群について解析した。DevSRに成分制御系は一酸化窒素をセンシングすることにより、いくつかの遺伝子の発現を制御していた。これらの遺伝子の破壊株は顕著に抗生物質生産に影響を与えた。
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Streptomyces coelicolor A3(2)における窒素酸化物受容タンパク質の解析
草間慎一郎、杉浦遼、田瀬有紀、矢嶋俊介、伊藤晋作、祥雲弘文、佐々木康幸
日本農芸化学会 2014.03
Event date： 2014.03
Language：Japanese Presentation type：Oral presentation (general)
Venue：東京
放線菌の生産する一酸化窒素受容体として二成分制御系DevSRを同定した。またこの受容体の制御する複数の遺伝子を同定した。 -
Streptomyces coelicolor A3(2)におけるSoxRの機能解析
田瀬有紀、小林卓矢、杉浦遼、草間慎一郎、矢嶋俊介、伊藤晋作、祥雲弘文、佐々木康幸
日本農芸化学会 2014.03
Event date： 2014.03
Language：English Presentation type：Oral presentation (general)
放線菌の生産する一酸化窒素の受容体の一つとして、SoxRを同定した。SoxR遺伝子の破壊によって本菌の形態に異常が顕著に表れ、一酸化窒素意義のひとつを示唆した。
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Streptomyces coelicolor A3(2)における生産物調整因子の解明
信太啓介、小口悠、棚橋月子、矢嶋俊介、伊藤晋作、祥雲弘文、佐々木康幸
日本農芸化学会 2014.03
Event date： 2014.03
Language：English Presentation type：Oral presentation (general)
Venue：東京
放線菌が生産する一酸化窒素はホルモンとして働いており、その生体内の濃度調節機構について解析した。生体内一酸化窒素の濃度は金体外に一旦放出される亜硝酸濃度に依存していた。この亜硝酸は亜硝酸還元酵素によりアンモニアに変換されることで調整される。