Title |
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Contact information |
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External Link |
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Mamoru TOTSUKA Professor |
From School 【 display / non-display 】
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The University of Tokyo Faculty of Agriculture Graduated
1984.04 - 1988.03
From Graduate School 【 display / non-display 】
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The University of Tokyo Graduate School, Division of Agricultural Science Doctor Course Unfinished Course
1990.04 - 1990.05
Country:Japan
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The University of Tokyo Graduate School of Agriculture Master Course Completed
1988.04 - 1990.03
Degree 【 display / non-display 】
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博士(農学) ( 1998.02 東京大学 )
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農学修士 ( 1990.03 東京大学 )
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Life Sciences Department of Molecular Microbiology Professor
2022.04
External Career 【 display / non-display 】
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Nippon Veterinary and Life Science University Faculty of Applied Life Science Professor
2017.10 - 2022.03
Country:Japan
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The University of Tokyo Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences Associate Professor
2006.04 - 2017.09
Country:Japan
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Ministry of Education, Culture, Sports, Science and Technology (MEXT) Research Promotion Bureau Senior Scientific Research Specialist
2009.08 - 2011.07
Country:Japan
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The University of Tokyo Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences Associate member associate professor
1998.06 - 2006.03
Country:Japan
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The University of Tokyo Department of Agricultural Chemistry, Faculty of Agiculture Research Assistant
1990.06 - 1998.05
Country:Japan
Professional Memberships 【 display / non-display 】
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日本農芸化学会
1988.12
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腸内細菌学会
2006.04
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日本食品免疫学会
2004.04
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日本動物細胞工学会
1990.04
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日本免疫学会
1992.04
Research Areas 【 display / non-display 】
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Life Science / Food sciences
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Life Science / Immunology
Research Interests 【 display / non-display 】
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Probiotics
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Intestinal Microbiota
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Gut Immunity
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Food Immunology
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Intestinal Epithelial Cell
Papers 【 display / non-display 】
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Michio Endo, Tadashi Yoshida, Keisatoi Ishii, Taku Iwamoto, Mamoru Totsuka, Makoto Hattori
Bioscience, Biotechnology, and Biochemistry 2022.12
Publishing type:Research paper (scientific journal) Publisher:Oxford University Press (OUP)
ABSTRACT
To reduce the immunogenicity of β-lactoglobulin (BLG), we prepared recombinant BLG which has both site-specific glycosylation and single amino acid substitution (D28N/P126A), and expressed it in the methylotrophic yeast Pichia pastoris by fusion of the cDNA to the sequence coding for the α-factor signal peptide from Saccharomyces cerevisiae. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) analysis indicated that the D28N/P126A was conjugated with a ∼4 kDa high-mannose chain. D28N/P126A retained ∼61% of the retinol-binding activity of BLG. Structural analyses by circular dichroism (CD) spectra, intrinsic fluorescence, and Enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies indicated that the surface structure of BLG was slightly changed by using protein engineering techniques, but D28N/P126A was covered by high-mannose chains and substituted amino acid without substantial disruption of native conformation. Antibody responses to the D28N/P126A considerably reduced in C57BL/6 mice. We conclude that inducing both site-specific glycosylation and single amino acid substitution simultaneously is an effective method to reduce the immunogenicity of BLG.DOI: 10.1093/bbb/zbac210
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Reduced immunogenicity of β-lactoglobulin by single amino acid substitution Reviewed
Tadashi Yoshida, Chisato Kume, Asako Sachi, Fumiko Yuyama, Naoko Tomiyama, Rina Kodama, Kiyoshi Yamada, Mamoru Totsuka, Makoto Hattori
Cytotechnology 74 ( 6 ) 623 - 634 2022.09
Publishing type:Research paper (scientific journal) Publisher:Springer Science and Business Media LLC
DOI: 10.1007/s10616-022-00549-9
Other Link: https://link.springer.com/article/10.1007/s10616-022-00549-9/fulltext.html
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A FRET biosensor for necroptosis uncovers two different modes of the release of DAMPs Reviewed
Shin Murai, Yoshifumi Yamaguchi, Yoshitaka Shirasaki, Mai Yamagishi, Ryodai Shindo, Joanne M. Hildebrand, Ryosuke Miura, Osamu Nakabayashi, Mamoru Totsuka, Taichiro Tomida, Satomi Adachi-Akahane, Sotaro Uemura, John Silke, Hideo Yagita, Masayuki Miura, Hiroyasu Nakano
Nature Communications 9 ( 1 ) 4457 2018.12
Language:English Publishing type:Research paper (scientific journal) Publisher:Springer Science and Business Media LLC
DOI: 10.1038/s41467-018-06985-6
Other Link: http://www.nature.com/articles/s41467-018-06985-6
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Anserine/carnosine supplementation preserves blood flow in the prefrontal brain of elderly people carrying APOE e4 Reviewed
Qiong Ding, Kitora Tanigawa, Jun Kaneko, Mamoru Totsuka, Yoshinori Katakura, Etsuko Imabayashi, Hiroshi Matsuda, Tatsuhiro Hisatsune
Aging and Disease 9 ( 3 ) 334 - 345 2018.06
Language:English Publishing type:Research paper (scientific journal) Publisher:International Society on Aging and Disease
In a previously reported double-blind, randomized controlled trial (RCT), we demonstrated that daily supplementation with anserine (750 mg) and carnosine (250 mg) improves brain blood flow and memory function in elderly people. Here, we conducted a sub-analysis of MRI data and test scores from the same RCT to determine whether anserine/carnosine supplementation specifically benefits elderly people carrying the APOE e4 allele, which is a risk gene for accelerated brain aging and for the onset of Alzheimer's Disease. We collected data from 68 participants aged 65 years or older who received anserine/carnosine supplementation (ACS) or placebo for 12 months. Subjects were assessed at the start and end of the trial using several neuropsychological tests, including the Wechsler Memory Scale-Logical Memory (WMS-LM). We also collected two types of MRI data, arterial spin labeling (ASL) and diffusion tensor imaging (DTI) at the start and end of the trial. We found that ACS significantly preserved verbal memory (WMS-LM, F[1,65] = 4.2003, p = 0.0445) and blood flow at frontal areas of the brain (FWEcluster level, p <
0.001). Sub-analysis based on the APOE4 genotype showed a significant preservation of blood flow (p = 0.002, by ASL analysis) and white-matter microstructure (p = 0.003, by DTI analysis) at prefrontal areas in APOE4+ subjects in the active group, while there was no significant difference between APOE4- subjects in the active and placebo groups. The effect of ACS in preserving brain structure and function in elderly people carrying APOE4 should be verified by further studies. -
Immunomodulation by food: Impact on gut immunity and immune cell function Invited Reviewed
Hachimura S, Totsuka M, Hosono A
Bioscience, Biotechnology and Biochemistry 82 ( 4 ) 584 - 599 2018.04
Authorship:Lead author Language:English Publishing type:Research paper (scientific journal)
Books and Other Publications 【 display / non-display 】
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日本食品免疫学会編集, 編集委員長:上野川修一, 編集副委員長:南野昌信, 八村敏志, 編集アドバイザー:伊藤裕之, 清野宏, 河野陽一, 清水誠, 高垣洋太郎, 田之倉優, 山本佳弘, 編集委員:市川寛, 北澤春樹, 國澤純, 後藤真生, 志田寛, 下條直樹, 園山慶, 高橋恭子, 高橋毅, 高橋宜聖, 立花宏文, 辻典子, 戸塚護, 永渕真也, 広瀬義隆, 細野朗, 八巻幸二( Role: Joint editor)
朝倉書店 2021.11 ( ISBN:9784254431261 )
Total pages:xviii, 474p Language:Japanese
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The Best Diet for Healthy Immune System to Prevent Viral Infections
Mamoru Totsuka( Role: Sole author)
2021.02 ( ISBN:9784594087081 )
Total pages:191p Language:Japanese
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21世紀のバイオサイエンス 実験農芸化学
戸塚護( Role: Contributor)
朝倉書店 2013.03
Language:Japanese Book type:Scholarly book
Misc 【 display / non-display 】
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免疫力を高める栄養と食事について Invited
戸塚 護
めいいく(㈱明治の食育情報誌) 10 2 - 3 2021.08
Authorship:Lead author Language:Japanese Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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戸塚護
メディアミルクセミナーニュースレター 51 1 - 4 2021.02
Language:Japanese Publishing type:Lecture material (seminar, tutorial, course, lecture, etc.)
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戸塚護
(一社)Jミルク ウェブサイト 2020.11
Language:Japanese Publishing type:Article, review, commentary, editorial, etc. (other)
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食による免疫機能、腸管機能の制御を目指して
戸塚護
腸内細菌学雑誌 34 ( 1 ) 19 - 22 2020.01
Authorship:Lead author Language:Japanese
Industrial Property Rights 【 display / non-display 】
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久恒 辰博, 戸塚 護, 薩 秀夫, 金子 順, 片倉 喜範, 佐藤 三佳子, 松本 貴之, 森松 文毅, 今林 悦子, 松田 博史
Application no:特願2019-019712 Date applied:2019.02
Announcement no:特開2019-070048 Date announced:2019.05
Country of applicant:Domestic
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久恒 辰博, 戸塚 護, 薩 秀夫, 金子 順, 片倉 喜範, 佐藤 三佳子, 松本 貴之, 森松 文毅, 今林 悦子, 松田 博史
Applicant:国立大学法人 東京大学, 国立大学法人九州大学, 日本ハム株式会社, 国立研究開発法人国立精神・神経医療研究センター
Application no:特願2014-142910 Date applied:2014.07
Announcement no:特開2015-193582 Date announced:2015.11
Country of applicant:Domestic
Honours, Awards and Prizes 【 display / non-display 】
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2020 BBB Most-Cited Review Award
2021.03 Japan Society for Bioscience, Biotechnology, and Agrochemistry
Satoshi Hachimura, Mamoru Totsuka, Akira Hosono
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Japanese Association for Food Immunology Award
2015.10 Japanese Association for Food Immunology
TOTSUKA Mamoru
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The Excellent Paper Award Published in Bioscience, Biotechnology, & Biochemistry
2010.03
Kiyoshi Yamada, Kanako Sato, Satoru Morishita, Shuichi Kaminogawa, Mamoru Totsuka
Scientific Research Funds Acquisition Results 【 display / non-display 】
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2015.04 - 2018.03
Grant-in-Aid for Scientific Research Basic Research (B)
Mamoru Totsuka
Authorship:Principal investigator
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Analysis of immunomodulatory function of probiotics and its enhancement with food factors
2012.04 - 2015.03
Grant-in-Aid for Scientific Research Basic Research (B)
Mamoru Totsuka
Authorship:Principal investigator
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Screening of food factors inducing regulatory B cells
2012.04 - 2014.03
Grant-in-Aid for Scientific Research Grant-in-Aid for challenging Exploratory Research
Mamoru Totsuka
Authorship:Principal investigator
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Molecular analysis of skin cell activation by orally administered functional food substances
2011.04 - 2014.03
Grant-in-Aid for Scientific Research Basic Research (B)
Makoto Shimizu
Authorship:Coinvestigator(s)
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2009.04 - 2012.03
Grant-in-Aid for Scientific Research Basic Research (B)
Mamoru Totsuka
Authorship:Principal investigator
Presentations 【 display / non-display 】
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食品成分による「免疫力」増強とアレルギー制御、 その基礎と最新動向 Invited
戸塚護
テックデザイン技術講習会 2021.08
Event date: 2021.08
Language:Japanese Presentation type:Public lecture, seminar, tutorial, course, or other speech
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Modulation of Immune / inflammatory reaction by food Invited
Mamoru Totsuka
2020.09
Event date: 2020.09
Language:Japanese Presentation type:Public lecture, seminar, tutorial, course, or other speech
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免疫機能を整えるための食生活と牛乳などの役割 Invited International conference
戸塚護
2020年度全国栄養士大会・オンライン 2020.08
Event date: 2020.08
Language:Japanese Presentation type:Oral presentation (general)
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Gut microflora and Health Invited
Mamoru Totsuka
2020.02
Event date: 2020.02
Language:Japanese Presentation type:Public lecture, seminar, tutorial, course, or other speech
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Basics of structure and function of the intestine and gut microbiota and evaluation of food function Invited
Mamoru Totsuka
2020.02
Event date: 2020.02
Language:Japanese Presentation type:Public lecture, seminar, tutorial, course, or other speech
Teaching Experience 【 display / non-display 】
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免疫学
Institution:Tokyo University of Agriculture
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食品免疫学
Institution:The University of Tokyo
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食品化学
Institution:The University of Tokyo
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食品機能化学
Institution:The University of Tokyo
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フードクリエーションサイエンス
Institution:The University of Tokyo
Committee Memberships 【 display / non-display 】
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腸内細菌学会 理事
2021.06
Committee type:Academic society
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日本食品免疫学会 幹事
2004.04
Committee type:Academic society
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日本食品免疫学会 広報編集委員長
2021.07
Committee type:Academic society
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日本農芸化学会 代議員
2016.04
Committee type:Academic society
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日本動物細胞工学会 評議員
2003.04
Committee type:Academic society
Social Activities 【 display / non-display 】
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Bioscience of Microbiota, Food and Health(BMFH)出版会 Associated Editor
Role(s): Edit
2019.11
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日本乳業協会 理事
2018.05
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牛乳乳製品健康科学会議 委員
2012.04
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茨城県立並木中等教育学校 スーパーサイエンスハイスクール運営指導委員
Role(s): Consultant
2012.04 - 2017.03