Updated on 2023/05/15

写真b

Mamoru TOTSUKA

Professor

Title

Professor

Contact information

Contact information

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From School 【 display / non-display

  • The University of Tokyo   Faculty of Agriculture   Graduated

    1984.04 - 1988.03

From Graduate School 【 display / non-display

  • The University of Tokyo   Graduate School, Division of Agricultural Science   Doctor Course   Unfinished Course

    1990.04 - 1990.05

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    Country:Japan

  • The University of Tokyo   Graduate School of Agriculture   Master Course   Completed

    1988.04 - 1990.03

Degree 【 display / non-display

  • 博士(農学) ( 1998.02   東京大学 )

  • 農学修士 ( 1990.03   東京大学 )

Employment Record in Research 【 display / non-display

  • Tokyo University of Agriculture   Faculty of Life Sciences   Department of Molecular Microbiology   Professor

    2022.04

External Career 【 display / non-display

  • Nippon Veterinary and Life Science University   Faculty of Applied Life Science   Professor

    2017.10 - 2022.03

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    Country:Japan

  • The University of Tokyo   Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences   Associate Professor

    2006.04 - 2017.09

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    Country:Japan

  • Ministry of Education, Culture, Sports, Science and Technology (MEXT)   Research Promotion Bureau   Senior Scientific Research Specialist

    2009.08 - 2011.07

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    Country:Japan

  • The University of Tokyo   Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences   Associate member associate professor

    1998.06 - 2006.03

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    Country:Japan

  • The University of Tokyo   Department of Agricultural Chemistry, Faculty of Agiculture   Research Assistant

    1990.06 - 1998.05

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    Country:Japan

Professional Memberships 【 display / non-display

  • 日本農芸化学会

    1988.12

  • 腸内細菌学会

    2006.04

  • 日本食品免疫学会

    2004.04

  • 日本動物細胞工学会

    1990.04

  • 日本免疫学会

    1992.04

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Research Areas 【 display / non-display

  • Life Science / Food sciences

  • Life Science / Immunology

Research Interests 【 display / non-display

  • Probiotics

  • Intestinal Microbiota

  • Gut Immunity

  • Food Immunology

  • Intestinal Epithelial Cell

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Papers 【 display / non-display

  • Site-specific glycosylation and single amino acid substitution dramatically reduced the immunogenicity of β-lactoglobulin

    Michio Endo, Tadashi Yoshida, Keisatoi Ishii, Taku Iwamoto, Mamoru Totsuka, Makoto Hattori

    Bioscience, Biotechnology, and Biochemistry   2022.12

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    Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    ABSTRACT

    To reduce the immunogenicity of β-lactoglobulin (BLG), we prepared recombinant BLG which has both site-specific glycosylation and single amino acid substitution (D28N/P126A), and expressed it in the methylotrophic yeast Pichia pastoris by fusion of the cDNA to the sequence coding for the α-factor signal peptide from Saccharomyces cerevisiae. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) analysis indicated that the D28N/P126A was conjugated with a ∼4 kDa high-mannose chain. D28N/P126A retained ∼61% of the retinol-binding activity of BLG. Structural analyses by circular dichroism (CD) spectra, intrinsic fluorescence, and Enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies indicated that the surface structure of BLG was slightly changed by using protein engineering techniques, but D28N/P126A was covered by high-mannose chains and substituted amino acid without substantial disruption of native conformation. Antibody responses to the D28N/P126A considerably reduced in C57BL/6 mice. We conclude that inducing both site-specific glycosylation and single amino acid substitution simultaneously is an effective method to reduce the immunogenicity of BLG.

    DOI: 10.1093/bbb/zbac210

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  • Reduced immunogenicity of β-lactoglobulin by single amino acid substitution Reviewed

    Tadashi Yoshida, Chisato Kume, Asako Sachi, Fumiko Yuyama, Naoko Tomiyama, Rina Kodama, Kiyoshi Yamada, Mamoru Totsuka, Makoto Hattori

    Cytotechnology   74 ( 6 )   623 - 634   2022.09

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10616-022-00549-9

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    Other Link: https://link.springer.com/article/10.1007/s10616-022-00549-9/fulltext.html

  • A FRET biosensor for necroptosis uncovers two different modes of the release of DAMPs Reviewed

    Shin Murai, Yoshifumi Yamaguchi, Yoshitaka Shirasaki, Mai Yamagishi, Ryodai Shindo, Joanne M. Hildebrand, Ryosuke Miura, Osamu Nakabayashi, Mamoru Totsuka, Taichiro Tomida, Satomi Adachi-Akahane, Sotaro Uemura, John Silke, Hideo Yagita, Masayuki Miura, Hiroyasu Nakano

    Nature Communications   9 ( 1 )   4457   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1038/s41467-018-06985-6

    Other Link: http://www.nature.com/articles/s41467-018-06985-6

  • Anserine/carnosine supplementation preserves blood flow in the prefrontal brain of elderly people carrying APOE e4 Reviewed

    Qiong Ding, Kitora Tanigawa, Jun Kaneko, Mamoru Totsuka, Yoshinori Katakura, Etsuko Imabayashi, Hiroshi Matsuda, Tatsuhiro Hisatsune

    Aging and Disease   9 ( 3 )   334 - 345   2018.06

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:International Society on Aging and Disease  

    In a previously reported double-blind, randomized controlled trial (RCT), we demonstrated that daily supplementation with anserine (750 mg) and carnosine (250 mg) improves brain blood flow and memory function in elderly people. Here, we conducted a sub-analysis of MRI data and test scores from the same RCT to determine whether anserine/carnosine supplementation specifically benefits elderly people carrying the APOE e4 allele, which is a risk gene for accelerated brain aging and for the onset of Alzheimer's Disease. We collected data from 68 participants aged 65 years or older who received anserine/carnosine supplementation (ACS) or placebo for 12 months. Subjects were assessed at the start and end of the trial using several neuropsychological tests, including the Wechsler Memory Scale-Logical Memory (WMS-LM). We also collected two types of MRI data, arterial spin labeling (ASL) and diffusion tensor imaging (DTI) at the start and end of the trial. We found that ACS significantly preserved verbal memory (WMS-LM, F[1,65] = 4.2003, p = 0.0445) and blood flow at frontal areas of the brain (FWEcluster level, p &lt
    0.001). Sub-analysis based on the APOE4 genotype showed a significant preservation of blood flow (p = 0.002, by ASL analysis) and white-matter microstructure (p = 0.003, by DTI analysis) at prefrontal areas in APOE4+ subjects in the active group, while there was no significant difference between APOE4- subjects in the active and placebo groups. The effect of ACS in preserving brain structure and function in elderly people carrying APOE4 should be verified by further studies.

    DOI: 10.14336/AD.2017.0809

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  • Immunomodulation by food: Impact on gut immunity and immune cell function Invited Reviewed

    Hachimura S, Totsuka M, Hosono A

    Bioscience, Biotechnology and Biochemistry   82 ( 4 )   584 - 599   2018.04

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/09168451.2018.1433017

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Books and Other Publications 【 display / non-display

  • 食品免疫学事典

    日本食品免疫学会編集, 編集委員長:上野川修一, 編集副委員長:南野昌信, 八村敏志, 編集アドバイザー:伊藤裕之, 清野宏, 河野陽一, 清水誠, 高垣洋太郎, 田之倉優, 山本佳弘, 編集委員:市川寛, 北澤春樹, 國澤純, 後藤真生, 志田寛, 下條直樹, 園山慶, 高橋恭子, 高橋毅, 高橋宜聖, 立花宏文, 辻典子, 戸塚護, 永渕真也, 広瀬義隆, 細野朗, 八巻幸二( Role: Joint editor)

    朝倉書店  2021.11  ( ISBN:9784254431261

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    Total pages:xviii, 474p   Language:Japanese  

  • The Best Diet for Healthy Immune System to Prevent Viral Infections

    Mamoru Totsuka( Role: Sole author)

    2021.02  ( ISBN:9784594087081

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    Total pages:191p   Language:Japanese  

  • 牛乳と健康ーわが国における研究の軌跡と将来展望「牛乳・乳製品と免疫調節」

    戸塚 護( Role: Contributor)

    ライフサイエンス出版  2015.02 

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    Language:Japanese  

  • 21世紀のバイオサイエンス 実験農芸化学

    戸塚護( Role: Contributor)

    朝倉書店  2013.03 

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    Language:Japanese   Book type:Scholarly book

  • 食品と免疫・アレルギー事典

    戸塚 護( Role: Contributor)

    朝倉書店  2011.05 

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    Language:Japanese  

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Misc 【 display / non-display

  • 免疫力を高める栄養と食事について Invited

    戸塚 護

    めいいく(㈱明治の食育情報誌)   10   2 - 3   2021.08

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

  • 免疫を調節する食品の役割

    戸塚護

    メディアミルクセミナーニュースレター   51   1 - 4   2021.02

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    Language:Japanese   Publishing type:Lecture material (seminar, tutorial, course, lecture, etc.)  

  • 免疫機能を整えるための 食生活と牛乳などの役割

    戸塚護

    (一社)Jミルク ウェブサイト   2020.11

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (other)  

  • 食による免疫機能、腸管機能の制御を目指して

    戸塚護

    腸内細菌学雑誌   34 ( 1 )   19 - 22   2020.01

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    Authorship:Lead author   Language:Japanese  

Industrial Property Rights 【 display / non-display

  • イミダゾールジペプチドを含む剤

    久恒 辰博, 戸塚 護, 薩 秀夫, 金子 順, 片倉 喜範, 佐藤 三佳子, 松本 貴之, 森松 文毅, 今林 悦子, 松田 博史

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    Application no:特願2019-019712  Date applied:2019.02

    Announcement no:特開2019-070048  Date announced:2019.05

    Country of applicant:Domestic  

  • イミダゾールジペプチドを含む剤

    久恒 辰博, 戸塚 護, 薩 秀夫, 金子 順, 片倉 喜範, 佐藤 三佳子, 松本 貴之, 森松 文毅, 今林 悦子, 松田 博史

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    Applicant:国立大学法人 東京大学, 国立大学法人九州大学, 日本ハム株式会社, 国立研究開発法人国立精神・神経医療研究センター

    Application no:特願2014-142910  Date applied:2014.07

    Announcement no:特開2015-193582  Date announced:2015.11

    Country of applicant:Domestic  

    J-GLOBAL

  • 遅延型過敏症軽減剤

    指原 紀宏, 池上 秀二, 戸塚 護, 吉田 綾子, 清水 誠

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    Applicant:株式会社明治

    Application no:特願2012-548826  Date applied:2011.12

    Patent/Registration no:特許第5937015号  Date registered:2016.05  Date issued:2016.05

    Country of applicant:Domestic   Country of acquisition:Domestic

    J-GLOBAL

  • 遅延型過敏症軽減剤

    指原 紀宏, 池上 秀二, 戸塚 護, 吉田 綾子, 清水 誠

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    Applicant:株式会社明治

    Application no:JP2011078986  Date applied:2011.12

    Announcement no:WO2012-081650  Date announced:2012.06

    Country of applicant:Domestic  

    J-GLOBAL

  • 免疫賦活用組成物

    茂呂 周, 岩瀬 孝志, 落合 邦康, 矢島 昌子, 寺原 正樹, 中村 吉孝, 戸塚 護, 山田 潔

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    Applicant:株式会社明治

    Application no:特願2007-503610  Date applied:2006.02

    Patent/Registration no:特許第5001830号  Date registered:2012.05  Date issued:2012.05

    Country of applicant:Domestic   Country of acquisition:Domestic

    J-GLOBAL

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Honours, Awards and Prizes 【 display / non-display

  • 2020 BBB Most-Cited Review Award

    2021.03   Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    Satoshi Hachimura, Mamoru Totsuka, Akira Hosono

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  • Japanese Association for Food Immunology Award

    2015.10   Japanese Association for Food Immunology  

    TOTSUKA Mamoru

  • The Excellent Paper Award Published in Bioscience, Biotechnology, & Biochemistry

    2010.03  

    Kiyoshi Yamada, Kanako Sato, Satoru Morishita, Shuichi Kaminogawa, Mamoru Totsuka

Scientific Research Funds Acquisition Results 【 display / non-display

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Presentations 【 display / non-display

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Teaching Experience 【 display / non-display

  • 免疫学

    Institution:Tokyo University of Agriculture

  • 食品免疫学

    Institution:The University of Tokyo

  • 食品化学

    Institution:The University of Tokyo

  • 食品機能化学

    Institution:The University of Tokyo

  • フードクリエーションサイエンス

    Institution:The University of Tokyo

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Committee Memberships 【 display / non-display

  • 腸内細菌学会   理事  

    2021.06   

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    Committee type:Academic society

  • 日本食品免疫学会   幹事  

    2004.04   

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    Committee type:Academic society

  • 日本食品免疫学会   広報編集委員長  

    2021.07   

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    Committee type:Academic society

  • 日本農芸化学会   代議員  

    2016.04   

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    Committee type:Academic society

  • 日本動物細胞工学会   評議員  

    2003.04   

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    Committee type:Academic society

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Social Activities 【 display / non-display

  • Bioscience of Microbiota, Food and Health(BMFH)出版会 Associated Editor

    Role(s): Edit

    2019.11

  • 日本乳業協会 理事

    2018.05

  • 牛乳乳製品健康科学会議 委員

    2012.04

  • 茨城県立並木中等教育学校 スーパーサイエンスハイスクール運営指導委員

    Role(s): Consultant

    2012.04 - 2017.03