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Title |
Associate Professor |
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Laboratory Address |
北海道網走市八坂196 |
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Laboratory Phone number |
0152-48-3906 |
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Contact information |
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External Link |
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KURODA Masamichi Associate Professor |
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Bio-Industry Department of Ocean and Fisheries Sciences Associate Professor
2025.04
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Tokyo University of Agriculture Faculty of Bio-Industry Department of Ocean and Fisheries Sciences Assistant Professor
2020.04 - 2025.03
External Career 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Bio-Industry Department of Ocean and Fisheries Sciences Assistant Professor
2020.04
Professional Memberships 【 display / non-display 】
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染色体学会
2018.05
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日本水産学会
2017.12
Papers 【 display / non-display 】
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Development of a repetitive DNA marker for identification of the exotic large-scale loach introduced to Japan Reviewed International journal
Masamichi Kuroda, Noriko Azuma, Takafumi Fujimoto, Katsutoshi Arai
Fisheries Science 2023.11
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Masamichi Kuroda, Noriko Azuma, Takaaki Shimizu, Takafumi Fujimoto, Katsutoshi Arai
Fisheries Science 2026.06
Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
Abstract
The large-scale loach Paramisgurnus dabryanus , introduced from continental Asia, coexists with the native dojo loach Misgurnus anguillicaudatus in Japan, likely causing genetic contamination of native populations through hybridization. Previous mitochondrial DNA (mtDNA) analyses have identified two divergent lineages of large-scale loach (groups 1 and 2), whereas nuclear DNA analysis of the RAG1 region revealed no differences between these two groups. In this study, we developed a novel mtDNA polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method targeting the cytochrome b region using Avr II and Nco I for rapid identification of groups 1 and 2 based on distinct fragment profiles. Consistent with previous research findings, nuclear DNA marker analyses revealed no differences between groups 1 and 2. This new marker enables efficient screening of large-scale loach lineages, facilitates distribution surveys, and clarifies the introduction pathways of this species.DOI： 10.1007/s12562-026-01990-x
Other Link： https://link.springer.com/article/10.1007/s12562-026-01990-x
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Nucleotide Sequence and Chromosome mapping of 5S Ribosomal DNA from the Dojo Loach, Misgurnus anguillicaudatus Reviewed International journal
Kiko Shibata, Masamichi Kuroda,· Etsuro Yamaha, · Katsutoshi Arai, Takafumi Fujimoto
Cytogenetic and Genome Research 2023.01
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Genetic differentiation in Palaemon septemtrionalis between populations of two rivers examined by newly developed short tandem repeat markers Reviewed
Noriko Azuma, Masamichi Kuroda, Yuichi Katogi, Susumu Chiba
Crustacean Research 51 123 - 127 2022.11
Language：English Publishing type：Research paper (scientific journal)
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Improvement in group identification of dojo loach, Misgurnus anguillicaudatus, using PCR‑restriction fragment length polymorphism Reviewed
Masamichi Kuroda,· Takafumi Fujimoto, · Etsuro Yamaha, · Katsutoshi Arai
Conservation Genetics Resources 2021.08
Language：English Publishing type：Research paper (scientific journal)
Honours, Awards and Prizes 【 display / non-display 】
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最優秀学生講演賞
2017.12 日本水産学会北海道支部
Award type：International academic award (Japan or overseas) Country：Japan
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佐々茂雄賞
2013.03 北海道大学
Award type：International academic award (Japan or overseas) Country：Japan
Scientific Research Funds Acquisition Results 【 display / non-display 】
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魚類の雑種化は配偶子形成にどのような影響を及ぼすのか？外来ドジョウ問題から解く
Grant number：2 3 K 1 4 0 1 2023 - 2025
日本学術振興会 科学研究費助成事業（学術研究助成基金助成金） 若手研究
Authorship：Principal investigator
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二倍性配偶子形成メカニズムの解明とその育種展開
2021.04 - 2024.03
科学研究費補助金 基盤研究(B)
藤本貴史
Authorship：Coinvestigator(s)
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三倍体ドジョウの配偶子形成を可能にする特殊なゲノム挙動の解明
2020 - 2021
科学研究費補助金 若手研究(スタートアップ)
黒田真道
Authorship：Principal investigator
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三倍体ドジョウの配偶子形成過程における特殊な染色体挙動と妊性に関する研究
2017.04 - 2020.03
科学研究費補助金 特別研究員奨励費
Other External Funds 【 display / non-display 】
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不妊魚のゲノム構成と倍数性がシナプトネマ複合体形成に与える影響の解明
2021 - 2022
民間財団等
黒田真道
Authorship：Principal investigator Grant type：Competitive
Presentations 【 display / non-display 】
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遺伝マーカーを用いた長野県における外来ドジョウの分布実態の解明
黒田真道・森重千秋・東典子・熊川真二
令和8年度日本水産学会春季大会 2026.03
Event date： 2026.03
Language：Japanese Presentation type：Oral presentation (general)
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サテライトDNAを用いた外来ドジョウ判別DNAマーカーの開発
森重千秋・黒田真道
令和７年度日本水産学会北海道支部大会 2025.12
Event date： 2025.12
Language：Japanese Presentation type：Oral presentation (general)
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Defective Meiotic Configurations Lead to Male Sterility in Clone-Origin Triploid and Hybrid Dojo Loach (Misgurnus anguillicaudatus) Invited
International Symposium on Loach Science 2025.12
Event date： 2025.12
Language：English
Country：China
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Gynogenesis and Polyploidy in Misgurnus anguillicaudatus: Cytogenetic Mechanisms Underlying Atypical Reproduction Invited
黒田真道
2024.12
Event date： 2024.12
Language：English Presentation type：Symposium, workshop panel (nominated)
Country：China
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ドジョウで見られる倍数性変異と特殊な生殖機構
第26回日本進化学会神奈川大会 2024.08
Event date： 2024.08
Presentation type：Symposium, workshop panel (nominated)
Teaching Experience 【 display / non-display 】
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水産動物発生学
Institution：Tokyo University of Agriculture
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基礎分子生物学
Institution：Tokyo University of Agriculture
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基礎遺伝学
Institution：Tokyo University of Agriculture