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YAMAMOTO Kosuke Associate Professor |
From School 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Bioindustry Graduated
1999.04 - 2003.03
Country：Japan
From Graduate School 【 display / non-display 】
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Tokyo University of Agriculture Graduate School, Division of Bioindustry Doctor Course Completed
2005.04 - 2009.03
Country：Japan
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Tokyo University of Agriculture Graduate School, Division of Bioindustry Master Course Completed
2003.04 - 2005.03
Country：Japan
Studying abroad experiences 【 display / non-display 】
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2011.09 - 2012.06 キール大学(Christian-Albrechts-Universität zu Kiel) 医学部 生化学科 ポストドクター
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2009.09 - 2011.08 キール大学(Christian-Albrechts-Universität zu Kiel) 医学部 生化学科 リサーチフェロー ドイツ学術交流会の奨学生としてドイツからの助成金を取得し、二年間国費留学
Degree 【 display / non-display 】
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博士(生物産業学) ( 2009.03 東京農業大学 )
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修士(生物産業学) ( 2005.03 東京農業大学 )
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学士(農学) ( 2003.03 東京農業大学 )
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Life Sciences Department of Molecular Microbiology Assistant Professor
2017.04 - 2020.03
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Tokyo University of Agriculture Faculty of Life Sciences Department of Molecular Microbiology Associate Professor
2020.04
External Career 【 display / non-display 】
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株式会社 先端生命科学研究所 研究開発部 研究員
2015.01
Country：Japan
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株式会社 医学生物学研究所 研究員
2012.07 - 2014.12
Country：Japan
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キール大学(Christian-Albrechts-Universität zu Kiel) 医学部 生化学科 リサーチフェロー
2011.09 - 2012.06
Country：Germany
Research Areas 【 display / non-display 】
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Others / Others / 基礎生物学
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Environmental Science/Agriculture Science / Landscape science / 環境農学
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Environmental Science/Agriculture Science / Environmental agriculture / 環境農学
Papers 【 display / non-display 】
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Noxious chemical discrimination by Tribolium castaneum TRPA1 channel in the HEK293 cell expression system Reviewed
Shimomura K., Oikawa H., Yamamoto K., Terajima T., Yajima S., Tomizawa M.
Current Research in Insect Science 4 100066 2023.07
Language：English Publishing type：Research paper (scientific journal)
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Hyphal Growth in Trichosporon asahii Is Accelerated by the Addition of Magnesium Reviewed
Keita Aoki, Kosuke Yamamoto, Moriya Ohkuma, Takashi Sugita, Naoto Tanaka, Masako Takashima
Microbiology Spectrum 2023.04
Language：English Publishing type：Research paper (scientific journal)
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Replacement of water yam (Dioscorea alata L.) indigenous root endophytes and rhizosphere bacterial communities via inoculation with a synthetic bacterial community of dominant nitrogen-fixing bacteria Reviewed International journal
Liswadiratanakul S, Yamamoto K, Matsutani M, Wattanadatsaree V, Kihara S, Shiwa Y and Shiwachi H
Front. Microbiol. 14 1060239 2023.03
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
Biofertilizers containing high-density plant growth-promoting bacteria are gaining interest as a sustainable solution to environmental problems caused by eutrophication. However, owing to the limitations of current investigative techniques, the selected microorganisms are not always preferred by the host plant, preventing recruitment into the native microbiota or failing to induce plant growth-promoting effects. To address this, five nitrogen-fixing bacteria previously isolated from water yam (Dioscorea alata L.) plants and showing dominant abundance of 1% or more in the water yam microbiota were selected for analysis of their plant growth-promoting activities when used as a synthetic bacterial inoculant. Water yam cv. A-19 plants were inoculated twice at 10 and 12 weeks after planting under greenhouse conditions. Bacterial communities in root, rhizosphere, and bulk soil samples were characterized using high-throughput 16S rRNA amplicon sequencing. Compared with non-inoculated plants, all bacterial communities were significantly altered by inoculation, mainly at the genus level. The inoculation effects were apparently found in the root communities at 16 weeks after planting, with all inoculated genera showing dominance (in the top 35 genera) compared with the control samples. However, no significant differences in any of the growth parameters or nitrogen contents were observed between treatments. At 20 weeks after planting, the dominance of Stenotrophomonas in the inoculated roots decreased, indicating a decline in the inoculation effects. Interestingly, only the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade was dominant (>1% relative abundance) across all samples, suggesting that bacteria related to this clade are essential core bacteria for water yam growth. This is the first report on addition of a synthetic nitrogen-fixing bacterial community in water yam plants showing that native bacterial communities can be replaced by a synthetic bacterial community, with declining in the effects of Stenotrophomonas on the modified communities several weeks after inoculation.
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Effects of Two Different Inoculation Methods Using a Synthetic Essential Core Bacterial Community on Water Yam (<i>Dioscorea alata</i> L.)
LISWADIRATANAKUL Sumetee, YAMAMOTO Kosuke, WATTANADATSAREE Vatanee, SHIWA Yuh, SHIWACHI Hironobu
Tropical Agriculture and Development 67 ( 2 ) 31 - 37 2023
Language：English Publisher：Japanese Society for Tropical Agriculture
<p>Biofertilizers containing plant growth–promoting bacteria are gaining interest as a sustainable solution to environmental problems caused by the inappropriate use of chemical compounds. However, the colonization and plant growth–promoting effects of inoculants can be affected by the method of inoculation. Therefore, this study aimed to compare the effects of two inoculation techniques—soil application and root dipping—using a synthetic bacterial community on water yam cv. A-19 growth under growth chamber conditions. Five plant growth–promoting bacteria in the <i>Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium</i> clade that were previously identified as essential core bacteria for water yam growth were used for synthetic bacterial inoculation. Compared with the root dipping technique, inoculation using the soil application technique significantly improved plant growth in terms of leaf number, area, and dry weight as well as SPAD value, suggesting that the soil application technique is an efficient method for bacterial inoculation in yam plants.</p>
DOI： 10.11248/jsta.67.31
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Diversity of endophytic bacterial microbiota in grapevine shoot xylems varies depending on wine grape-growing region, cultivar, and shoot growth stage Reviewed
Kazuhiro Hamaoka , Yoshinao Aoki , Sayuri Takahashi , Shinichi Enoki , Kosuke Yamamoto , Keisuke Tanaka , Shunji Suzuki
Scientific Reports 12 ( 1 ) 15772 2022.09
Language：English Publishing type：Research paper (scientific journal)
Books and Other Publications 【 display / non-display 】
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次世代シークエンサーDRY解析教本 改訂第2版, 応用編14 メタ16Sシークエンスの各サンプルから得られた細菌叢組成の差を主座標分析・クラスター分析により可視化する
山本紘輔 志波優（ Role： Joint author）
学研メディカル秀潤社 2019.12
Language：Japanese Book type：Scholarly book
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Plant acetylcholinesterase plays an important role in the response to environmental stimuli. In Neurotransmitters in plants: perspectives and applications, edited by Akula Ramakrishna, Victoria Vladimirovna Roshchina
Yamamoto K* and Momonoki YS（ Role： Joint author , 全頁 (全実験データ取得および論文執筆を担当)。筆頭著者及びコレスポンディングオーサーとして寄与。 ）
CRC press 2018.11
Language：English Book type：Scholarly book
Misc 【 display / non-display 】
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ドイツでの研究生活について Invited
山本 紘輔
ECHO (ドイツ学術交流協会DAAD友の会会報) 39 11 - 14 2023.11
Authorship：Lead author, Corresponding author Language：Japanese
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植物のアセチルコリンを介するシグナル伝達系の解明 Invited
山本 紘輔
アグリバイオ 7 ( 9 ) 65 - 68 2023.08
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Industrial Property Rights 【 display / non-display 】
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TUMOR MARKER MEASUREMENT METHOD AND MEASUREMENT REAGENT
YAMAMOTO, Kosuke; KITAMURA, Yoshiyuki; YAGI, Shintaro; AOYAGI, Katsumi
Application no：PCT/JP2017/031874 Date applied：2017.09
Publication no：WO/2018/047793 Date published：2018.03
Country of applicant：Domestic
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METHOD AND REAGENT FOR MEASURING THYROGLOBULIN
YAMAMOTO, Kosuke; KITAMURA, Yoshiyuki; YAGI, Shintaro; AOYAGI, Katsumi
Application no：PCT/JP2017/031869 Date applied：2017.09
Publication no：WO/2018/047792 Date published：2018.03
Country of applicant：Domestic
Scientific Research Funds Acquisition Results 【 display / non-display 】
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植物における新規アセチルコリン受容体遺伝子の同定と分子機能の解析
2022.04
科学研究費補助金 基盤研究(C)
山本 紘輔
Authorship：Principal investigator
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新しい植物神経伝達様システムの解明
2017.09
科学研究費補助金 研究活動スタート支援
山本紘輔
Authorship：Principal investigator
Other External Funds 【 display / non-display 】
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The Role of Interleukin-6 Classical- and Trans-Signaling in Inflammation and Cancer
2009.09 - 2011.08
Foreign Countries
Grant type：Competitive
Presentations 【 display / non-display 】
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Bacterial diversity associated with the rhizosphere and endosphere of two halophytes: Glaux maritima and Salicornia europaea International conference
Kosuke Yamamoto*, Yuh Shiwa, Taichiro Ishige, Hikaru Sakamoto, Keisuke Tanaka, Masataka Uchino, Naoto Tanaka, Suguru Oguri, Hiromasa Saitoh, Seiya Tsushima
2019 IS-MPMI (International Society for Molecular Plant-Microbe Interactions) XVIII Congress 2019.07
Event date： 2019.07
Language：English Presentation type：Oral presentation (general)
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Illumina-based analysis of Rhizosphere and Endosphere Bacterial Communities related to Halophytes Glaux maritima and Salicornia europaea International conference
Kosuke Yamamoto, Yuh Shiwa, Taichiro Ishige, Hikaru Sakamoto, Masataka Uchino, Naoto Tanaka, Suguru Oguri, Hiromasa Saitoh, Seiya Tsushima
32 nd ANNUAL MEETING of JAPANESE SOCIETY for MICROBIAL ECOLOGY & 10 th ASIAN SYMPOSIUM on MICROBIAL ECOLOGY 2018.07
Event date： 2018.07
Language：Japanese Presentation type：Oral presentation (general)
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アセチルコリンとアセチルコリンエステラーゼの気孔運動に対する影響
斎藤 生、菅原 拓登、山本 紘輔、桃木 芳枝、小栗 秀、坂本 光
日本植物生理学会学会第54回大会 2013.03
Event date： 2013.03
Language：Japanese Presentation type：Poster presentation
Venue：岡山大学
野生型タバコの気孔開閉へのAChの影響と、アッケシソウAChE過剰発現タバコ(SeAChEタバコ)の表現型を調べた。野生型タバコにAChのみを与えても気孔開度は変化しないが、気孔閉鎖誘導物質アブシジン酸(ABA)とAChを同時に与えた場合、ABAによる気孔閉鎖が緩和された。対照的に、SeAChEタバコでは気孔開度が常に低下していた。AChはABAシグナル系を抑制する効果を持ち、SeAChEタバコでは内在性AChが分解されることで、ABA感受性が高まり気孔閉鎖が促進されていることが考えられた。
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植物アセチルコリンエステラーゼの細胞内局在性
小林智也、山本紘輔、小栗秀、桃木 芳枝
日本植物生理学会学会第51回大会 2010.03
Event date： 2010.03
Language：Japanese Presentation type：Poster presentation
Venue：熊本大学
トウモロコシAChEのアミノ酸配列と77%および66％の相同性を有する2種類のイネAChEホモログ(rice AChE1およびAChE2)を発見した。Rice AChE1プロモーター下流にGFP遺伝子を導入し、プロモーター活性の組織局在性を検討した。その結果、表皮組織でGFPシグナルが観察された。
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イネにおけるアセチルコリンエステラーゼの同定および機能解析
山本紘輔、志田智史、小林智也、小栗秀、桃木 芳枝
日本植物生理学会学会第50回大会 2009.03
Event date： 2009.03
Language：Japanese Presentation type：Oral presentation (general)
Venue：名古屋大学
トウモロコシAChEのアミノ酸配列と77%および66％の相同性を有する2種類のイネAChEホモログ(rice AChE1およびAChE2)を発見し，イネにおいて各ホモログ遺伝子を植物バイオテクノロジー技術で過剰発現およびノックダウンした．Rice AChE1およびAChE2を過剰発現した形質転換イネは，ベクターコントロールと比較して極めて高いAChE活性を示した．そのため，両rice AChEホモログ遺伝子はイネにおけるAChE遺伝子であり，イネには2種類のAChEアイソザイムが存在することが示唆された．また，rice AChE1およびAChE2を過剰発現およびノックダウンした形質転換イネを用いて，熱および重力などの環境ストレスに対する両AChEの応答を比較検討した．