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Title |
Professor |
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Laboratory Phone number |
0152-48-3855 |
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External Link |
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HIRAYAMA Hiroki Professor |
From School 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Bioindustry Graduated
1991.04 - 1995.03
Country：Japan
From Graduate School 【 display / non-display 】
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Tokyo University of Agriculture Graduate School, Division of Bioindustry Doctoral program (first term) Completed
- 1997.03
Country：Japan
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Bio-Industry Department of Bio-production Associate Professor
2015.04 - 2016.09
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Tokyo University of Agriculture Faculty of Bio-Industry Department of Bio-production Professor
2016.10 - 2018.03
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Tokyo University of Agriculture Faculty of Bio-Industry Department of Northern Biosphere Agriculture Professor
2018.04
External Career 【 display / non-display 】
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北海道立総合研究機構畜産試験場 基盤研究部 主査
2012.04 - 2015.03
Country：Japan
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北海道立総合研究機構畜産試験場 基盤研究部 研究主任
2010.04 - 2012.03
Country：Japan
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北海道立畜産試験場 畜産工学部 研究員
2000.04 - 2010.03
Country：Japan
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株式会社新薬開発研究所 薬理研究部 研究員
1997.04 - 2000.03
Country：Japan
Professional Memberships 【 display / non-display 】
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The International Embryo Technology Society
2019.09
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Society for the Study of Reproduction
2004.06
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日本繁殖生物学会
2002.06
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北海道草地畜産学会
2000.09
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日本畜産学会
1998.01
Research Areas 【 display / non-display 】
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Life Science / Veterinary medical science
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Life Science / Animal production science
Papers 【 display / non-display 】
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Calving prediction with continuous measurement of subcutaneous tissue glucose concentration in pregnant cows Reviewed International journal
Wakatsuki, Takuji Nakamura, Tsukasa Ishii, Ayumi Konishi, Kanta Okubo, Michiko Souma, Kousaku Hirayama, Hiroki
Theriogenology 202 119 - 124 2023
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
To reduce losses of dams and calves due to unfortunate events, such as dystocia and freezing to death, identifying the onset of calving and providing necessary assistance are crucial. Prepartum increase in blood glucose concentration is a known indicator to detect labor in pregnant cows. However, some issues, including the need for frequent blood sampling and stress on cows, must be resolved before establishing a method for anticipating calving using changes in blood glucose concentrations. Herein, instead of measuring the blood glucose concentrations, subcutaneous tissue glucose concentration (tGLU) was measured in peripartum primiparous (n = 6) and multiparous (n = 8) cows at 15 min intervals using a wearable sensor. A transient increase in tGLU was observed in the peripartum period, with peak individual concentrations occurring between 2.8 h before and 3.5 h after calving. tGLU in primiparous cows was significantly higher than that in multiparous cows. To account for individual variations in basal tGLU, the maximum relative increase in the 3-h moving average of tGLU (Max MA) was used to predict calving. Cutoff points for Max MA were established by parity, with receiver operating characteristic analysis predicting calving within 24, 18, 12, and 6 h. Except for one multiparous cow that showed an increase in tGLU just before calving, all cows reached at least two cutoff points and calving was predicted successfully. The time interval between reaching the tGLU cutoff points that predicted calving within 12 h and actual calving was 12.3 ± 5.6 h. In conclusion, this study demonstrated the potential role of tGLU as a predictive indicator of calving in cows. Advancements in machine learning-based prediction algorithms and bovine-optimized sensors will help in increasing the accuracy of calving prediction using tGLU.
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Impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cows Reviewed International journal
INABA Ryo, KAWAHARA-MIKI Ryouka, SHINOZAWA Akihisa, YASUHARA Taichi, FUJII Takashi, KOYAMA Keisuke, MURATA-OKUBO Michiko, SOUMA Kousaku, HIRAYAMA Hiroki
Journal of Reproduction and Development 68 ( 1 ) 30 - 37 2022
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：The Society for Reproduction and Development
<p> Although hormonal induction of parturition in cattle results in the successful delivery of healthy calves, the risk of retained fetal membrane is significantly increased. In a previous study, a combination of the long-acting glucocorticoid, triamcinolone acetonide, with a high dose of betamethasone partially normalized the placentomal gene expression during parturition; however, the incidence of retained fetal membrane remained high. This study further explored placentomal dysfunction and aimed to elucidate the mechanism of retained fetal membrane in parturition-induced cows. In this study, transcriptome analysis revealed that enhanced glucocorticoid exposure normalized the expression of a substantial fraction of genes in the cotyledons. In contrast, a significant reduction in the multiple signaling pathway activities, including interferon signaling, was found in the caruncles during induced parturition. Real-time PCR showed that the expression of interferon-tau in the caruncles, but not interferon-alpha or interferon-gamma, was significantly lower in induced parturition than spontaneous parturition. Interferon-stimulated gene expression was also significantly decreased in the caruncles during induced parturition. These results indicate that interferon signaling could be important for immunological control in placentomes during parturition. Additionally, this suggests that interferon-tau might be a pivotal ligand for interferon receptors in the caruncles. This study revealed that peripheral blood leukocytes in prepartum cows transcribed interferon-tau. Macrophage infiltration in the placentome is known to participate in the detachment of the fetal membrane from the caruncle. Thus, this study raised the possibility that immune cells migrating into the caruncles at parturition may act as a source of ligands that activate interferon signaling.</p>
DOI： 10.1262/jrd.2021-094
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Expression of C-C motif chemokines and their receptors in bovine placentomes at spontaneous and induced parturition. Reviewed International journal
Hirayama, Hiroki Sakumoto, Ryosuke Koyama, Keisuke Yasuhara, Taichi Hasegawa, Taito Inaba, Ryo Fujii, Takashi Naito, Akira Moriyasu, Satoru Kageyama, Soichi
The Journal of reproduction and development 2020
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Mitochondrial DNA variations in Japanese farmed emu populations Reviewed International journal
KOSHIISHI Yuichi, UKITA Misuzu, MURATA-OKUBO Michiko, FUJISAWA Shin-ichiro, SHIMOI Gaku, HIRAYAMA Hiroki, KAMEYAMA Yuichi, SOUMA Kousaku, WADA Kenta
Journal of Veterinary Medical Science 82 ( 6 ) 731 - 734 2020
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
<p>The emu (<i>Dromaius novaehollandiae</i>) is a new poultry. In this study, we investigated the haplotype composition of mitochondrial DNA among emu populations farmed in Japan. We sequenced the D-loop region in 109 individuals, and detected four substitution sites and three haplotypes (Hap-a, -b, and -c). Hap-a was the most frequently observed haplotype in the Japanese populations. Although Hap-c was a rare haplotype in not only Japanese but also Australian populations, it was detected with high frequency in the Japanese farmed population. The AMOVA indicated that 9% of total variance was “among population”. The <i>F</i><sub>ST</sub> value was 0.087 and genetic differentiation was significant (<i>P</i><0.01). These results may contribute to conserving the genetic resources available for the Japanese emu industry.</p>
DOI： 10.1292/jvms.19-0693
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Development and characterization of ten novel microsatellite loci for the emu (Dromaius novaehollandiae) and genetic diversity of Japanese farm populations. Reviewed International journal
Koshiishi, Yuichi Murata-Okubo, Michiko Fujisawa, Shin-Ichiro Shimoi, Gaku Hirayama, Hiroki Kameyama, Yuichi Souma, Kousaku Wada, Kenta
Molecular biology reports 2020
Language：English Publishing type：Research paper (scientific journal)
Misc 【 display / non-display 】
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Embryo sexing and sex chromosomal chimerism analysis by loop-mediated isothermal amplification in cattle and water buffaloes Invited
Hirayama H, Kageyama S, Moriyasu S, Sawai K, Minamihashi A
J Reprod Dev 59 321 - 326 2013.08
Authorship：Lead author Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Sexing of bovine preimplantation embryos using loop-mediated isothermal amplification (LAMP) Invited
Kageyama S, Hirayama H
J Mamm Ova Res 29 113 - 118 2012.11
Authorship：Lead author Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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新しい遺伝子増幅法（LAMP法）の応用と展開 Invited
平山博樹
日本胚移植学雑誌 27 21 - 22 2005.01
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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新しい遺伝子増幅法（LAMP法）による牛胚の性判別
陰山聡一, 平山博樹
日本胚移植学雑誌 25 2003.09
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)
Honours, Awards and Prizes 【 display / non-display 】
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Studies on rapid preimplantation genomic diagnosis of bovine embryos
2012.09
Hiroki Hirayama
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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新規遺伝子増幅法（LAMP法）による牛受精卵性判別キットの研究開発
2004.01 中央畜産会
北海道立畜産試験場 遺伝子診断応用研究グループ
Award type：Award from publisher, newspaper, foundation, etc. Country：Japan
Scientific Research Funds Acquisition Results 【 display / non-display 】
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ウシ抗ミューラー管ホルモンの繁殖生理制御分子としての役割
2020.04 - 2023.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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牛の精漿蛋白質による子宮機能調節機序の解明と受胎性改善技術への応用
2019.04 - 2023.03
科学研究費補助金 基盤研究(A)
Authorship：Coinvestigator(s)
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牛の分娩時胎盤節における炎症誘導機構の解明と胎盤成熟誘導型分娩誘起技術開発
2016.04 - 2020.03
科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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牛における胎盤成熟メカニズムの解明と分娩誘起法の改良
2013.04 - 2016.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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X染色体不活化機構を介した核リプログラミングの機構解明とクローンウシ作出の効率化
2011.04 - 2013.03
科学研究費補助金 特定領域研究
Authorship：Coinvestigator(s)
Other External Funds 【 display / non-display 】
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ウシ二次卵胞の発育制御におけるTNFα受容体の役割
2021.06 - 2022.03
民間財団等
Authorship：Principal investigator Grant type：Competitive
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牛の小型ピロプラズマ病に対する感染抵抗性遺伝子の同定
2019.06 - 2020.03
民間財団等
Authorship：Principal investigator Grant type：Competitive
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牛伸長胚を用いた受精卵ゲノム選抜による高効率種雄牛造成技術
2015.08 - 2016.02
民間財団等 ２１世紀ＣＯＥプログラム
Authorship：Principal investigator Grant type：Competitive
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牛伸長胚を利用した現場でできる性判別技術の開発
2014.08 - 2015.02
民間財団等
Authorship：Coinvestigator(s) Grant type：Competitive
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牛の生理機能を活かした安定的過剰排卵技術の開発
2008.04 - 2009.03
民間財団等 ２１世紀ＣＯＥプログラム
Authorship：Principal investigator Grant type：Competitive