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MATSUSHIMA Yoshitaka Professor |
From School 【 display / non-display 】
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The University of Tokyo Faculty of Agriculture Graduated
- 1995.03
Country：Japan
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The University of Tokyo Faculty of Agriculture Graduated
- 1992.03
Country：Japan
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The University of Tokyo Faculty of Agriculture Graduated
- 1990.03
Country：Japan
Employment Record in Research 【 display / non-display 】
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Tokyo University of Agriculture Faculty of Applied Bio-Science Department of Applied Biology and Chemistry Professor
2014.04 - 2018.03
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Tokyo University of Agriculture Faculty of Applied Bio-Science Department of Agricultural Chemistry Professor
2018.04
External Career 【 display / non-display 】
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浜松医科大学医学部医学科 准教授
2007.04 - 2014.03
Country：Japan
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浜松医科大学医学部医学科 助教授
2003.04 - 2007.03
Country：Japan
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東京工業大学理学部化学科 助手
1996.03 - 2003.03
Country：Japan
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東京大学大学院農学生命科学研究科応用生命科学専攻 日本学術振興会特別研究員
1995.04 - 1996.03
Country：Japan
Research Areas 【 display / non-display 】
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Life Science / Bioorganic chemistry / Natural Products Chemistry
Papers 【 display / non-display 】
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TAMAKI Mizuna, CHIKU Tsutomu, SUZUKI Shunya, MISAKI Aya, FUNAKUBO Asuka, MATSUSHIMA Yoshitaka, YOKOTA Kenji, IGIMI Shizunobu, KAJIKAWA Akinobu
Bioscience of Microbiota, Food and Health 44 ( 1 ) 70 - 79 2025
Language：English Publishing type：Research paper (scientific journal) Publisher：BMFH Press
<p>Although the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas system has been extensively developed since its discovery for eukaryotic and prokaryotic genome editing and other genetic manipulations, there are still areas warranting improvement, especially regarding bacteria. In this study, BRD0539, a small-molecule inhibitor of <i>Streptococcus pyogenes</i> Cas9 (SpCas9), was used to suppress the activity of the nuclease during genetic modification of <i>Lacticaseibacillus paracasei</i>, as well as to regulate CRISPR interference (CRISPRi). First, we developed and validated a CRISPR-SpCas9 system targeting the <i>sirA</i> gene of <i>L. paracasei</i>. Then BRD0539 was used for CRISPR-dependent DNA cleavage <i>in vivo</i>. Our results suggested that the inhibitor worked partially in both <i>Escherichia coli</i> and <i>L. paracasei</i>. Next, we designed a CRISPRi system in a <i>L. paracasei</i> strain by inserting an inactive SpCas9 gene into the chromosome and introducing a plasmid encoding for a single guide RNA (sgRNA) targeting the <i>sirA</i> gene. Expression of <i>sirA</i> was successfully inhibited in the recombinant strains, and CRISPRi was abolished in an inhibitor-dependent manner. Our findings may help expand the CRISPR toolbox for research on lactic acid bacteria and other microbes.</p>
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Gram-scale approach for β-costic acid via allylic oxidation of β-selinene Reviewed International journal
Yoshitaka Matsushima, Kosuke Iwata
Bioscience, Biotechnology, and Biochemistry 88 ( 7 ) 742 - 746 2024.05
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Gram-scale synthesis of cytoxazone via dihydrooxazine formation through SiO2-promoted intramolecular substitution of a trichloroacetimidate Reviewed International journal
Yoshitaka Matsushima, Kota Otsuka, Moe Ishikawa, Shu Akita
Tetrahedron Letters 131 154586 2023.10
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Simple synthesis of violaceoid A, a cytotoxic hydroquinone Reviewed International journal
Yoshitaka Matsushima, Haruka Yoshioka, Naoya Sunada
Tetrahedron Letters 122 154522 2023.04
Language：English Publishing type：Research paper (scientific journal)
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Concise synthesis of amino acid component of amicoumacins via dihydrooxazine formation through intramolecular conjugate addition Reviewed International journal
Yoshitaka Matsushima, Yukako Ogawa, Katsuya Nishi, Kyosuke Nakata
Bioscience, Biotechnology, and Biochemistry 87 ( 2 ) 131 - 137 2023.02
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
Books and Other Publications 【 display / non-display 】
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松島芳隆，渡邊総一朗，古荘義雄（ Role： Joint author）
化学同人 2022.04 （ ISBN:9784759820560 ）
Language：Japanese Book type：Scholarly book
Misc 【 display / non-display 】
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有機化学の教科書と教育にいま何が求められているか Invited
松島芳隆，渡邊総一朗，古荘義雄
化学 77 ( 6 ) 42 - 45 2022.05
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
Other External Funds 【 display / non-display 】
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DNAアダクトーム解析のためのDNA付加体およびアミダイトの合成研究
2017.04
公益財団法人 喫煙科学研究財団
松島芳隆
Authorship：Principal investigator Grant type：Competitive