Papers - KAMEYAMA Yuichi
-
Post-ovulatory oocyte aging affects the initial differentiation into trophectderm lineage after fertilization in mice.（共著） Reviewed International journal
Shimoi G, Moronaga C, Jozawa R, Kudoh K, and Kameyama Y
Journal of Mammalian Ova Research 40 ( 2 ) 57 - 69 2023.11
Language：English Publishing type：Research paper (scientific journal)
-
Breeding of long-clawed shrews, <i>Sorex unguiculatus</i>, in captivity Reviewed
Okimoto Kohei, D. Ohdachi Satoshi, Katsuyama Yuriko, Shimoi Gaku, Wada Kenta, Kameyama Yuichi
Honyurui Kagaku (Mammalian Science) 63 ( 2 ) 143 - 155 2023.07
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Mammal Society of Japan
<p>This report describes the breeding of long-clawed shrews under laboratory conditions. During pairing, females waggled their waists, causing horizontal shaking of the tail, and walked around the males. Mounting was observed on the second day of pairing. In the two pairs with successful subsequent deliveries, mounting was observed 51 and 9 times with average (±<i>SD</i>) durations of 39 ± 59 and 56 ± 54 s, respectively. Similar multiple-mounting behavior was also observed in those pairs for which mating attempts proved unproductive. The gestational period was approximately 21–22 days. Weaning was successful in 4 of the 12 observed deliveries, and the weaning period was approximately 31–36 days. During the lactation period, the females constructed dome-like nests using straw mulch. The activity time of lactating mothers increased as the lactation period advanced. Weanlings attained almost the same body size as subadults, although their body weight did not increase until nine weeks after weaning.</p>
-
Genetic relationships among emu populations in Japanese farms based on mitochondrial and microsatellite DNA polymorphisms（共著） Reviewed
Koshiishi Y, Murata-Okubo M, Kameyama Y, Souma K, Hirayama H and Wada K
Anim. Sci. J. 93 ( e13786 ) 1 2022.11
Language：English Publishing type：Research paper (scientific journal)
-
Gender-difference in hair length as revealed by crispr-based production of long-haired mice with dysfunctional FGF5 mutations（共著） Reviewed
Takahashi R, Takahashi G, Kameyama Y, Sato M, Ohtsuka M and Wada K
Int. J. Mol. Sci. 23 ( 11855 ) 1 2022.10
Language：English Publishing type：Research paper (scientific journal)
-
Carcass traits and fat quality of breeding emu ( Dromaius novaehollandiae ) in Northern Japan（共著） Reviewed
Koshiishi Y, Murata‑Okubo M, Nakajyo H, Machida R, Yamamoto T, Myoda T, Kameyama Y, Hirayama H, Souma K, Yokohama M and Wada K
Poult. Sci. 101 ( 102050 ) 1 - 8 2022.08
Language：English Publishing type：Research paper (scientific journal)
-
Effects of post-ovulatory aging on centrometric cohesin protection in murine MII oocytes（共著） Reviewed
Shimoi G, Wakabayashi R, Ishikawa R and Kameyama Y
Reproductive Medicine and Biology 21 ( e12433 ) 1 - 10 2021.11
Language：English Publishing type：Research paper (scientific journal)
IVFやICSIに伴って生じる染色体障害の検出を目的として、DNA損傷の指標である姉妹染色体分体交換（SCE）の検出を試み、胚操作が初期胚や産仔の染色体に及ぼす細胞遺伝学的な影響について検証した。配送さによって重度のDNA損傷を誘発した胚は、発生の比較的初期の段階で淘汰され、出産には至らない可能性が示唆された。
-
Development of 49 novel microsatellite markers from Next-generation sequencing data and a robust method for parentage tests in the emu ( Dromaius novaehollandiae ) （共著） Reviewed
Koshiishi Y, Uchiyama H, Murata‑Okubo M, Tanaka K, Kameyama Y, Hirayama H and Wada K
Gene 769 ( 145238 ) 1 - 7 2020.10
Language：English Publishing type：Research paper (scientific journal)
-
Mitochondrial DNA variations in Japanese farmed emu populations Reviewed
KOSHIISHI Yuichi, UKITA Misuzu, MURATA-OKUBO Michiko, FUJISAWA Shin-ichiro, SHIMOI Gaku, HIRAYAMA Hiroki, KAMEYAMA Yuichi, SOUMA Kousaku, WADA Kenta
Journal of Veterinary Medical Science 82 ( 6 ) 731 - 734 2020.06
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
<p>The emu (<i>Dromaius novaehollandiae</i>) is a new poultry. In this study, we investigated the haplotype composition of mitochondrial DNA among emu populations farmed in Japan. We sequenced the D-loop region in 109 individuals, and detected four substitution sites and three haplotypes (Hap-a, -b, and -c). Hap-a was the most frequently observed haplotype in the Japanese populations. Although Hap-c was a rare haplotype in not only Japanese but also Australian populations, it was detected with high frequency in the Japanese farmed population. The AMOVA indicated that 9% of total variance was “among population”. The <i>F</i><sub>ST</sub> value was 0.087 and genetic differentiation was significant (<i>P</i><0.01). These results may contribute to conserving the genetic resources available for the Japanese emu industry.</p>
DOI： 10.1292/jvms.19-0693
-
Development and characterization of ten novel microsatellite loci for the emu (Dromaius novaehollandiae) and genetic diversity of Japanese farm populations（共著） Reviewed
Koshiishi Y, Murata‑Okubo M, Fujisawa S, Shimoi G, Hirayama H, Kameyama Y, Souma K and Wada K
Molecular Biology Reports 48 ( 4 ) 1 - 7 2020.02
Language：English Publishing type：Research paper (scientific journal)
To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers.
-
Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes（共著） Reviewed
Shimoi G, Tomita M, Kataoka M and Kameyama Y
Journal of Reproduction and Development 65 ( 1 ) 57 - 66 2019.02
Language：English Publishing type：Research paper (scientific journal)
IVFやICSIに伴って生じる染色体障害の検出を目的として、DNA損傷の指標である姉妹染色体分体交換（SCE）の検出を試み、胚操作が初期胚や産仔の染色体に及ぼす細胞遺伝学的な影響について検証した。配送さによって重度のDNA損傷を誘発した胚は、発生の比較的初期の段階で淘汰され、出産には至らない可能性が示唆された。
-
A 1-bp deletion in Fgf5 causes male-dominant long hair in the Syrian hamster（共著） Reviewed
Yoshizawa Y, Wada K, Shimoi G, Kameyama Y, Wakabayashi Y, Fukuta K and Hashizume R
Mammalian Genome 26 ( 11 ) 630 - 637 2015.12
Language：English Publishing type：Research paper (scientific journal)
To our knowledge, this report is the first to present the sexual dimorphism of hair length caused by the Fgf5 mutation
-
Characterization and application of newly developed polymorphic microsatellite markers in the Ezo red fox (Vulpes vulpes schrencki)（共著） Reviewed
Tada T, Seki Y, Kameyama Y, Kikkawa Y and Wada K
Genetics and Molecular Research 15 ( 4 ) 1 - 6 2015.12
Language：English Publishing type：Research paper (scientific journal)
To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers.
-
北海道網走市におけるオオアシトガリネズミ Sorex unguiculatusの寄生虫相（共著） Reviewed
亀山祐一，佐藤理恵，浅川満彦，伊東拓也，沖本康平，下井 岳，橋詰良一
東京農大農学集報 60 ( 1 ) 10 - 17 2015.06
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
北海道網走市の山林で捕獲したオオアシトガリネズミを対象に、駆虫を行う前段として寄生虫を採集、同定した。
-
体毛から抽出したDNAを用いた オオアシトガリネズミSorex unguiculatusの性判別（共著） Reviewed
亀山祐一，黒田高光，木村 慧，沖本康平，下井 岳，橋詰良一
東京農大農学集報 59 ( 2 ) 163 - 167 2014.09
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
生体から反復して簡便に採取できる体毛を材料として、PCRによるオオアシトガリネズミの性判別法を確立した。
-
L-アルギニンが体外におけるマウス精子の受精能に及ぼす影響 Reviewed
下井 岳、亀山祐一、橋詰良一、伊藤雅夫
日本受精着床学会雑誌 28 ( 2 ) 344 - 349 2011.08
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
一酸化窒素（NO）の基質となるL-アルギニンが体外で精子の受精能獲得に及ぼす影響について検証した。
-
過重力負荷培養を伴ったマウス体外受精のおける透明帯穿孔法の導入－低濃度精子における受精率向上の試み－ Reviewed
下井 岳、工藤謙一、亀山祐一、橋詰良一、伊藤雅夫
日本受精着床学会雑誌 28 ( 2 ) 372 - 377 2011.08
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
遠心重力負荷を伴ったIVF（g-IVF）法にZDを導入することで、極めて低い精子濃度においても安定した受精成績を得ることが可能であるかを検証した。
-
Asymmetrical allocation of mitochondrial DNA to blastomeres during the first two cleavages in mouse embryos （共著） Reviewed
Kameyama Y, Ohnishi H, Shimoi G, Hashizume R, Ito M and Smith LC
Reproduction, Fertility and Development 22 ( 8 ) 1247 - 1253 2010.10
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
A recent report showed higher oxygen consumption, adenosine triphosphate (ATP) production and mitochondrial localisation in trophectoderm cells than in the inner cell mass of mouse blastocysts. We hypothesised that this phenomenon was due to the asymmetrical distribution of mitochondria in the blastomeres during the earlier stages. Oocytes, 2-cell embryos and 4-cell embryos were analysed to determine the volume, ATP content and mitochondrial DNA (mtDNA) copy number in the whole egg and individual blastomeres. Significant differences were detected in the volumes of cytoplasm and ATP contents between blastomeres from the 2-cell and 4-cell embryos. Moreover, whilst remaining stable in whole embryos, mtDNA copy number differed between blastomeres, indicating that mitochondria in oocytes are unevenly delivered into the daughter blastomeres during the first two cleavages. Although their volume and ATP content were not correlated, there was a significant correlation between volume and mtDNA copy number in 2- and 4-cell blastomeres. These results indicate that the number of mitochondria delivered to blastomeres during early cleavage is not precisely equal, suggesting that the allocation of mitochondria into daughter blastomeres is affected by uneven cytoplasmic distribution during cytokinesis in the oocyte and mother blastomeres.
DOI： 10.1071/RD10076
-
Sister chromatid exchange frequency in early embryo and offspring derived from IVF or ICSI in mice（共著） Reviewed
Shimoi G, Kameyama T, Kudoh K, Hasgizume R and Ito M
Journal of Mammalian Ova Research 27 ( 3 ) 114 - 121 2010.10
Language：English Publishing type：Research paper (scientific journal)
IVFやICSIに伴って生じる染色体障害の検出を目的として、DNA損傷の指標である姉妹染色体分体交換（SCE）の検出を試み、胚操作が初期胚や産仔の染色体に及ぼす細胞遺伝学的な影響について検証した。配送さによって重度のDNA損傷を誘発した胚は、発生の比較的初期の段階で淘汰され、出産には至らない可能性が示唆された。
-
北海道オホーツク海域に生息する魚介類における重金属蓄積量の検証（共著）
下井 岳、藤田悠記、亀山祐一、鈴木淳志、橋詰良一、伊藤雅夫
東京農業大学農学集報 52 ( 3 ) 141 - 146 2007.12
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
北海道のオホーツク海域に生息する魚介類における重金属の蓄積量について、重金属の主たる蓄積臓器である肝臓と可食部について分析を行い、オホーツク海における重金属汚染状況を検証した。オホーツク海域の重金属が人体へ与える影響はきわめて低いことが示された。
全頁数：6 -
遠心で分離したマウス卵母細胞断片の特性（共著） Reviewed
亀山祐一、下井 岳、海老原克仲、後藤千恵、五十嵐愛、伊藤雅夫
哺乳動物卵子学会誌 24 ( 4 ) 167 - 174 2007.10
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
哺乳動物の卵母細胞を透明帯除去、細胞骨格阻害剤処理後に遠心すると、無核と有核の細胞断片に分離する。この卵母細胞断片を核移植や細胞質移植に使えれば、ドナー核体、レシピエント細胞質体、ドナー細胞質体の調整が容易になる。本研究ではマウス卵母細胞から遠心で細胞断片を調製する方法と得られた卵母細胞断片の細胞学的な特性について検討した。透明帯除去卵母細胞をサイトカラシンD（CD）処理後、個々にパーコール非連続密度勾配を持つキャピラリに詰めて遠心分離した。回収した卵母細胞断片について染色質、ミトコンドリア、脂肪顆粒の分布を観察した。卵母細胞を4～8℃でCD処理した後に1分間遠心すると、57％が1対の小細胞断片（平均直径27.6 um）と大細胞断片（平均直径73.5 um）に分離した。大細胞断片は90%以上が無核で、ミトコンドリアの分布に伴う強く均質な蛍光を示した。卵母細胞の脂肪顆粒は遠心の求心側に移動し、その一部が小細胞断片に取り込まれて分離したと思われる。これらの大細胞断片は核移植のレシピエント細胞質体として利用可能と思われた。
全頁数：8