論文 - 志和地 弘信
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Replacement of Water Yam (Dioscorea alata L.) Indigenous Root Endophytes and Rhizosphere Bacterial Communities via Inoculation with a Synthetic Bacterial Community of Dominant Nitrogen-Fixing Bacteria 査読あり
Sumetee Liswadiratanakul, Kosuke Yamamoto, Minenosuke Mastutani, Vatanee Wattanadatsaree, Shunta Kihara, Yuh Shiwa and Hironobu Shiwachi
Frontiers in Microbiology. 2023年02月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Ryo Matsumoto, Asrat Asfaw, Haruki Ishikawa, Kanako Takada, Hironobu Shiwachi, Robert Asiedu
Frontiers in Plant Science. 2022年10月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：https://www.frontiersin.org
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Bacterial community of water yam (Dioscorea alata L.) cv. A-19 査読あり 国際誌
Kihara, Shunta, Yamamoto Kosuke, Hisatomi Atsushi, Shiwa Yuh, Chu Chia-Cheng, Takada Kanako, Ouyabe Michel, Pachakkil Babil, Kikuno Hidehiko, Tanaka Naoto, Shiwachi Hironobu
Microbes and Environments 37 ( 2 ) ME21062 2022年03月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Growth of Water Yam (Dioscorea alata L.) as Affected by Bacterial Inoculation under Growth Chamber Conditions. 査読あり
Liswadiratanakul Sumetee, Michel Ouyabe, Hideki Hinomizu, Kanako Takada, Hidehiko Kikuno, Babil Pachakkil and Hironobu Shiwachi
Tropical Agriculture and Development 65 ( 1 ) 41 - 48 2021年03月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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<sup>15</sup>N希釈法によるホワイトギニアヤムの窒素固定量の推定
髙田 花奈子, 松本 亮, 石川 春樹, Pachakkil Babil, 菊野 日出彦, 志和地 弘信
日本作物学会講演会要旨集 249 ( 0 ) 60 - 60 2020年03月
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Infection Route of Endophytic Nitrogen-fixing Bacteria in Water Yam (Dioscorea alata L.) 査読あり
Hironobu Shiwachi, Natsumi Miwa, Kanako Takada, Michel Ouyabe, Babil Pachakkil, Hidehiko Kikuno, and Naoto Tanaka
Tropical Agriculture and Development 64 ( 1 ) 1 - 6 2020年03月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.11248/jsta.64.1
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Response of upland rice (Oryza sativa L.) inoculated with non-native plant growth-promoting bacteria 査読あり
Michel Ouyabe, Irie Kenji, Tanaka Naoto, Hidehiko Kikuno, Babil Pachakkil and Hironobu Shiwachi
Agronomy 10 2020年
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Michel Ouyabe, Naoto Tanaka, Yuh Shiwa, Nobuyuki Fujita, Hidehiko Kikuno, Pachakkil Babil and Hironobu Shiwachi
International Journal of Systematic and Evolutionary Microbiology 70 ( 9 ) 5054 - 5062 2020年
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Microbiology Society
<jats:p>This study investigated endophytic nitrogen-fixing bacteria isolated from two species of yam (water yam, <jats:italic>Dioscorea alata</jats:italic> L.; lesser yam, <jats:italic>Dioscorea esculenta</jats:italic> L.) grown in nutrient-poor alkaline soil conditions on Miyako Island, Okinawa, Japan. Two bacterial strains of the genus <jats:italic>
<jats:named-content content-type="genus">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1279" xlink:type="simple">Rhizobium</jats:ext-link>
</jats:named-content>
</jats:italic>, S-93<jats:sup>T</jats:sup> and S-62, were isolated. The phylogenetic tree, based on the almost-complete 16S rRNA gene sequences (1476 bp for each strain), placed them in a distinct clade, with <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13566" xlink:type="simple">Rhizobium miluonense</jats:ext-link>
</jats:named-content>
</jats:italic> CCBAU 41251<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1287" xlink:type="simple">Rhizobium hainanense</jats:ext-link>
</jats:named-content>
</jats:italic> I66<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13567" xlink:type="simple">Rhizobium multihospitium</jats:ext-link>
</jats:named-content>
</jats:italic> HAMBI 2975<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.24765" xlink:type="simple">Rhizobium freirei</jats:ext-link>
</jats:named-content>
</jats:italic> PRF 81<jats:sup>T</jats:sup> and <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1306" xlink:type="simple">Rhizobium tropici</jats:ext-link>
</jats:named-content>
</jats:italic> CIAT 899<jats:sup>T</jats:sup> being their closest species. Their bacterial fatty acid profile, with major components of C<jats:sub>19 : 0</jats:sub> cyclo <jats:italic>ω</jats:italic>8<jats:italic>c</jats:italic> and summed feature 8, as well as other phenotypic characteristics and DNA G+C content (59.65 mol%) indicated that the novel strains belong to the genus <jats:italic>
<jats:named-content content-type="genus">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1279" xlink:type="simple">Rhizobium</jats:ext-link>
</jats:named-content>
</jats:italic>. Pairwise average nucleotide identity analyses separated the novel strains from their most closely related species with similarity values of 90.5, 88.9, 88.5, 84.5 and 84.4 % for <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13567" xlink:type="simple">R. multihospitium</jats:ext-link>
</jats:named-content>
</jats:italic> HAMBI 2975<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1306" xlink:type="simple">R. tropici</jats:ext-link>
</jats:named-content>
</jats:italic> CIAT 899<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1287" xlink:type="simple">R. hainanense</jats:ext-link>
</jats:named-content>
</jats:italic> CCBAU 57015<jats:sup>T</jats:sup>, <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13566" xlink:type="simple">R. miluonense</jats:ext-link>
</jats:named-content>
</jats:italic> HAMBI 2971<jats:sup>T</jats:sup> and <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.24765" xlink:type="simple">R. freirei</jats:ext-link>
</jats:named-content>
</jats:italic> PRF 81<jats:sup>T</jats:sup>, respectively; digital DNA–DNA hybridization values were in the range of 26–42 %. Considering the phenotypic characteristics as well as the genomic data, it is suggested that strains S-93<jats:sup>T</jats:sup> and S-62 represent a new species, for which the name <jats:italic>Rhizobium dioscoreae</jats:italic> is proposed. The type strain is S-93<jats:sup>T</jats:sup> (=NRIC 0988<jats:sup>T</jats:sup>=NBRC 114257<jats:sup>T</jats:sup>=DSM 110498<jats:sup>T</jats:sup>).</jats:p> -
Isolation of Nitrogen-Fixing Bacteria from Water Yam (Dioscorea alata L.) 査読あり
Kanako TAKADA, Naoto TANAKA, Hidehiko KIKUNO, Pachakkil BABIL, Michio ONJO, Byoung-Jae PARK, and Hironobu SHIWACHI
Tropical Agriculture and Development. 63 63 ( 4 ) 198 - 203 2019年12月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Contribution of Biological Nitrogen Fixation in Lesser Yam (Dioscorea esculenta L.) Associated with Endophytic Diazotrophic Bacteria 査読あり
Michel OUYABE, Hidehiko KIKUNO, Naoto TANAKA, Pachakkil BABIL, Hironobu SHIWACH
Tropical Agriculture and Development 63 ( 3 ) 131 - 139 2019年09月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Endophytic Nitrogen-Fixing Bacteria of Water Yam (Dioscorea alata L.) in Relation with Fertilization Practices 査読あり
Michel OUYABE, Hidehiko KIKUNO, Naoto TANAKA, Pachakkil BABIL, Hironobu SHIWACH
Tropical Agriculture and Development 63 ( 3 ) 122 - 130 2019年09月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Isolation and identification of nitrogen-fixing bacteria associated with Dioscorea alata L. and Dioscorea esculenta L 査読あり
Michel OUYABE, Hidehiko KIKUNO, Naoto TANAKA, Pachakkil BABIL, Hironobu SHIWACH
Microbial Resources and Systematics 35 ( 1 ) 3 - 11 2019年06月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Bulbil dormancy and formation in water yam (Dioscorea alata L.) 査読あり
Atsushi Sanada, Chuwen Cheng, Hidehiko Kikuno and Hironobu Shiwachi
Tropical Agriculture and Development 62 ( 3 ) 109 - 114 2018年09月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Analysis of the source of nitrogen during water yam (Dioscorea alata L.) growth using δ15N observations 査読あり
Kanako Takada, Hidehiko Kikuno, Pachakkil Babil and Hironobu Shiwachi
Tropical Agriculture and Development 62 ( 3 ) 124 - 131 2018年09月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Intra –Specific Genetic Diversity in Dioscorea alata accessions collected from Myanmar, Papua New Guinea and Japan. 査読あり
Favour OLU-OLUSEGUN, Pachakkil BABIL, Hidehiko KIKUNO, Kenji IRIE, Hidekazu TOYOHARA, and Hironobu SHIWACHI
Journal of International Society for Southeast Asian Agricultural Sciences 24 ( 1 ) 107 - 117 2018年06月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Water yam (Dioscorea alata L.) is able to grow in low fertile soil conditions 査読あり
Kanako TAKADA, Hidehiko KIKUNO, Pachakkil BABIL, Kenji IRIE and Hironobu SHIWACHI
Tropical Agriculture and Development 61 ( 1 ) 8 - 14 2017年03月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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The nitrogen-fixing endophytes bacterium associated with the lesser yam (Dioscorea esculenta L.) 査読あり
Abdul Qayom Rezaei, Hidehiko Kikuno, Pachakkil Babil, Naoto Tanaka, Byoung-Jae Parak, Michio Onjo and Hironobu Shiwachi
Tropical Agriculture and Development 61 ( 1 ) 40 - 47 2017年03月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Phylogenetic Relationship of Indonesian Water Yam (Dioscorea alata L.) Cultivars Based on DNA Marker Using ITS-rDNA Analysis 査読あり
Purnomo, Budi Setiadi Daryono and Hironobu Shiwachi
Journal of Agricultural Science 9 ( 2 ) 154 - 161 2017年01月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Somatic polyploidization and characterization of induced polyploids of Dioscorea rotundata and Dioscorea cayenensis 査読あり
Pachakkil Babil1, Midori Iino, Yukiko Kashihara, Ryo Matsumoto, Hidehiko Kikuno,Antonio Lopez-Montes and Hironobu Shiwachi
African Journal of Biotechnology 15 ( 38 ) 2098 - 2105 2016年09月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effect of Nitrogen Fertilizer on Growth of the Lesser Yam (Dioscorea esculenta L.) 査読あり
Abdul Qayom Rezaei, Hidehiko Kikuno, Kaoru Sugiyama, Pachakkil Babil, Byoung-Jae Parak, Michio Onjo and Hironobu Shiwachi
Tropical Agriculture and Development 60 ( 3 ) 185 - 190 2016年09月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
The effect of fertilization on lesser yam (Dioscorea esculenta L.) growth is not clearly understood. Increased lesser
yam production in Okinawa prefecture, Japan, is desired due to increasing demand for use in commercial health supplements.
However, fertilization guidelines for production are not yet available in Japan. This study aimed to reveal the effects of nitrogen application on growth of lesser yam in Okinawa from April 2014 to January 2015. Growth with urea application (N treatment)and without urea (control) were compared. N treatment had a positive effect on growth and tuber yield in soil with poor organic matter. Yellowish leaves were observed in control plants from 120 days after planting (DAP), but plants grew until 240 DAP. It was possible to grow lesser yams under poor fertile soil conditions. The δ15N values of the N treated plants suggested that nitrogen was absorbed from the urea; however, the source of nitrogen in the control plants was not identified. The quantity of nitrogen required for a stable yield of lesser yam is thought to be less than that required for water yam (D. alata).