Papers - YOSHIKAWA Jun
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Erythritol in miso and rice koji is produced by koji mold Reviewed International journal
Jun Yoshikawa*, Sayo Matsunami, Chihiro Kubota, Momoka Fukuda, Mayumi Maeda, Kenji Maehashi
Food Science and Technology Research 31 ( 5 ) 419 - 425 2025.09
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Isomaltooligosaccharides production using α-glucosidase activity from Zalaria sp. Him3, a fructooligosaccharides-producing yeast Reviewed International journal
Haruki Matsuya, Mayumi Maeda, Kenji Maehashi, Jun Yoshikawa
Current Microbiology 82 427 2025.08
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer-Nature
DOI： 10.1007/s00284-025-04392-x
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Shiitake mushroom (Lentinula edodes) extract has chloride-activated glutaminase activity that efficiently increases glutamic acid in foods with sodium chloride Reviewed International journal
Kana Takegami, Mayumi Maeda, Jun Yoshikawa, and Kenji Maehashi
International Journal of Gastronomy and Food Science 38 10154 2024.11
Language：English Publishing type：Research paper (scientific journal)
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D-Arabitol production by a high arabitol-producing yeast, Zygosaccharomyces sp. Gz-5 isolated from miso Reviewed International journal
Kan Iwata, Rikuo Kanokozawa, Aoi Iwata, Mayumi Maeda, Kenji Maehashi, Jun Yoshikawa*
Bioscience, Biotechnology, and Biochemistry 88 ( 9 ) 1102 - 1108 2024.09
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford academic
DOI： 10.1093/bbb/zbae075
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Flavor assessment of a lactic fermented vinegar described in Japanese books from the Edo period (1603–1867) Reviewed
Naoyuki Yanagihara, Mayumi Maeda, Jun Yoshikawa, Sayuri Akuzawa, Akira Fujii, Masanobu Nagano, Yukimichi Koizumi, Kenji Maehashi*
Heliyon 10 ( 11 ) E32344 2024.06
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Isolation of Zygosaccharomyces siamensis kiy1 as a novel arabitol‑producing yeast and its arabitol production Reviewed International journal
Kan Iwata, Mayumi Maeda, Yutaka Kashiwagi, Kenji Maehashi, Jun Yoshikawa*
AMB Express 13 76 2023.07
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Nature
DOI： 10.1186/s13568-023-01581-4
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De novo genome assembly and analysis of Zalaria sp. Him3, a novel fructooligosaccharides producing yeast Reviewed International journal
Yoshikawa J.*, Matsutani M., Maeda M., Kashiwagi Y., Maehashi K.
BMC Genomic DATA 23 78 2022.11
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Decolorization of cationic dyes under alkaline conditions by Iodidimonas sp. Q-1 multicopper oxidase Reviewed International journal
Ebihara K., Yoshikawa J., Horiguchi H., Amachi S.
Journal of Bioscience and Bioengineering 133 ( 4 ) 323 - 328 2022.03
Language：English Publishing type：Research paper (scientific journal)
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Isolation and identification of Zalaria sp. Him3 as a novel fructooligosaccharides-producing yeast Reviewed International journal
Yoshikawa J.*, Honda Y., Saito Y., Sato D., Iwata K., Amachi S., Kashiwagi Y., Maehashi K.
Journal of Applied Microbiology 132 ( 2 ) 1104 - 1111 2022.02
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1111/jam.15259
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Identification and characterization of proteinase B as an unstable factor for neutral lactase in the enzyme preparation from Kluyveromyces lactis. Reviewed
Sugawara A., Horiguchi H., Yoshikawa J.*
Journal of Bioscience and Bioengineering 131 ( 1 ) 20 - 26 2021.01
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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The role of amylolytic and proteolytic enzyme activities of vegetables, fruits, and edible fungi in flavor enhancement during cooking. Reviewed
Oosone S., Kashiwaba A., Yanagihara N., Yoshikawa J., Kashiwagi Y., Maehashi K.
International Journal of Gastronomy and Food Science 22 ( 100264 ) 1 - 9 2020.09
Language：English Publishing type：Research paper (scientific journal)
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One-step removal of proteases in a commercial lactase preparation from Kluyveromyces lactis using an anion-exchange membrane. Reviewed
Yoshikawa J.*, Shiota K., Horiguchi H.
Food Science and Technology Research 26 ( 1 ) 65 - 68 2020.03
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.3136/fstr.26.65
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Enzyme induction during ethanol metabolism in a methylotrophic yeast, Candida sp. N-16 (in Japanese) Reviewed
Yoshikawa J., Koyama M., Seki K., Fujii T., and Amachi S.
HortResearch 74 1 - 5 2019.11
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
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Simple method for analyzing the purity of protease-containing samples by acid-treatment SDS-PAGE. Reviewed
Fujita T., Shiota K., Yoshikawa J., Ogawa S., Aoyagi H.
Journal of Bioscience and Bioengineering 128 ( 5 ) 630 - 635 2019.05
Language：English Publishing type：Research paper (scientific journal)
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Decolorization of recalcitrant dyes by a multicopper oxidase produced by Iodidimonas sp. Q-1 with iodide as a novel inorganic natural redox mediator. Reviewed
Taguchi T., Ebihara K., Yanagisaki C., Yoshikawa J., Horiguchi H., Amachi S.
Scientific Reports 8 ( 6717 ) 2018.04
Language：English Publishing type：Research paper (scientific journal)
Iodidomonas sp. Q-1株由来のマルチ銅オキシダ－ゼは、I-をI2に酸化する。I-をメディエーターとして本酵素を利用することで、様々な色素や染色工場の実廃液を脱色できることが示された。さらに、本脱色システムは、市販の糸状菌由来酵素よりも効果的であることが分かった。
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Synthesis of an allergy inducing tetrasaccharide “4P-X”. Reviewed
Moriya T., Nagahata N., Odaka R., Nakamura H., Yoshikawa J., Kurashima K., Saito T.
Carbohydrate Research 439 44 - 49 2017.02
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
既存のガラクトオリゴ糖（GOS）には、アレルゲン性を有する４糖（4P-X）が含まれる可能性が報告されている。GOSに関連する研究を行うに当たり、4P-Xの含有を調査するための標準品とするため、4P-Xを酵素的ならびに有機化学的な手法を組み合わせることにより合成した。
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Production of D-arabitol from raw glycerol by Candida quercitrusa. Reviewed
Yoshikawa J., Habe H., Morita T., Fukuoka T., Imura T., Iwabuchi H., Uemura S., Tamura T., Kitamoto D.
Applied Microbiology and Biotechnology 98 ( 7 ) 2947 - 2953 2014.07
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
バイオディーゼルの副産物である廃グリセロールを利用するため、有用糖質であるD-アラビトール生産菌C. quercitrusaを取得した。本菌によって、活性炭処理を行った廃グリセロールから40%の変換効率で、85.1 g/LのD-アラビトールを生産できた。
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Production of mannitol from raw glycerol by Candida azyma. Reviewed
Yoshikawa J., Habe H., Morita T., Fukuoka T., Imura T., Iwabuchi H., Uemura S., Tamura T., Kitamoto D.
Journal of Bioscience and Bioengineering 117 ( 6 ) 725 - 729 2014.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
バイオディーゼルの副産物である廃グリセロールを利用するため、有用糖質であるマンニトール生産菌C. azymaを取得した。本菌によって、活性炭処理を行った廃グリセロールから30%の変換効率で、50.8 g/Lのマンニトールを生産できた。
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Selective production of two diastereomers of disaccharide sugar alcohol, mannosylerythritol by Pseudozyma yeasts. Reviewed
Yoshikawa J., Morita T., Fukuoka T., Imura T., Konishi M., Kakugawa K., Kitamoto D.
Applied Microbiology and Biotechnology 98 ( 2 ) 823 - 830 2014.02
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
新規糖アルコールであるマンノシルエリスリトール(ME)を生産するため、Pseudozyma属酵母からスクリーニングを行った。2種類の菌を用いることでMEのジアステレオマーそれぞれを選択的に生産することができた。
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Iodide oxidation by a novel multicopper oxidase from the alphaproteobacterium strain Q-1. Reviewed
Suzuki M., Eda Y., Ohsawa S., Kanesaki Y., Yoshikawa H., Tanaka K., Muramatsu Y., Yoshikawa J., Sato I., Fujii T., Amachi S.
Applied Environmental Microbiology 78 ( 11 ) 3941 - 3949 2012.11
Language：English Publishing type：Research paper (scientific journal)
ヨウ素酸化細菌であるQ-1株が生産するヨウ素酸化酵素の一つを精製して調べたところ、本酵素は分子内に銅原子を含有し、ドラフトゲノム配列と内部アミノ酸配列からマルチ銅オキシダーゼのファミリーであることが示唆された。
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Isolation and properties of malic enzyme and its gene in Rhodopseudomona palustris No.7. Reviewed
Sato I., Yoshikawa J., Furusawa A., Chiku K., Amachi S., Fujii T.
Bioscience, Biotechnology and Biochemistry 74 ( 1 ) 75 - 81 2010.01
Language：English Publishing type：Research paper (scientific journal)
紅色非硫黄細菌であるR. palustris No.7の炭素代謝について重要であると考えられているmalic enzymeを精製、遺伝子情報を取得し、性質を調べた。
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Production of fructooligosaccharides by using crude enzyme preparation of β-fructofuranosidase from Aureobasidium pullulans DSM 2404. Reviewed
Yoshikawa J., Amachi S., Shinoyama H., Fujii T.
Biotechnology Letters 30 ( 3 ) 535 - 539 2008.03
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
A. pullulans DSM 2404のβ-フラクトフラノシダーゼⅠを多く含む粗酵素標品と市販のグルコース異性化酵素を用いてフラクトオリゴ糖生産反応を行ったところ、従来よりも高い収率が得られた。
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Purification and some properties of β-fructofuranosidase I formed by Aureobasidium pullulans DSM 2404. Reviewed
Yoshikawa J., Amachi S., Shinoyama H., Fujii T.
Journal of Bioscience and Bioengineering 103 ( 5 ) 491 - 493 2007.05
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
A. pullulans DSM 2404において、β-フラクトフラノシダーゼⅠを精製したところ、他の酵素と比較して性質が異なっていた。さらに、フラクトオリゴ糖の生産性を調べたところ、高い値を示した。
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Aureobasidium pullulans DSM 2404におけるβ-フラクトフラノシダーゼの解析とフラクトオリゴ糖の生産に関する研究
吉川潤
2007.03
Language：Japanese Publishing type：Doctoral thesis
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Multiple β- fructofuranosidases by Aureobasidium pullulans DSM2404 and their roles in fructooligosaccharide production. Reviewed
Yoshikawa J., Amachi S., Shinoyama H., Fujii T.
FEMS Microbiology Letters 265 ( 2 ) 159 - 163 2006.12
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
5種類のβ-フラクトフラノシダーゼが検出されたA. pullulans DSM 2404において、フラクトオリゴ糖の蓄積時期と分解時期には異なる酵素が生産され、それらは糖転移能に違いが見られた。
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Purification and Properties of Two Malate Dehydrogenases from Candida sp. N-16 Grown on Methanol. Reviewed
Yoshikawa J., Seki K., Shinoyama H., Fujii T.
Bioscience, Biotechnology and Biochemistry 65 ( 7 ) 1659 - 1662 2001.07
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
メタノール資化性酵母Candida sp. N-16より、構成酵素であるMDH-M1とメタノール特異的に誘導されるMDH-M2の両酵素を精製し、酵素化学的性質を比較した。
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メタノール資化性酵母におけるリンゴ酸脱水素酵素のアイソザイム
吉川潤
1999.03
Language：Japanese Publishing type：Master’s thesis