Updated on 2023/09/20

写真a

TORII Yasushi

Professor

Title

Professor

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Degree 【 display / non-display

  • 博士(獣医学) ( 2010.03   大阪府立大学 )

Employment Record in Research 【 display / non-display

  • Tokyo University of Agriculture   Faculty of Agriculture   Department of Animal Science   Associate Professor

    2015.04 - 2018.03

  • Tokyo University of Agriculture   Faculty of Agriculture   Department of Animal Science   Associate Professor

    2018.04 - 2019.03

  • Tokyo University of Agriculture   Faculty of Agriculture   Department of Animal Science   Professor

    2019.04

Papers 【 display / non-display

  • Genetic Diversity of <i>BoLA-DRB3</i> in Japanese Brown Cattle

    NISHIKAKU Kohei, INENAGA Toshiaki, NISHI Akihito, YONEZAWA Takahiro, NOGUCHI Tatsuo, TORII Yasushi, IMAKAWA Kazuhiko, KOBAYASHI Tomoko

    Journal of Veterinary Epidemiology   26 ( 2 )   108 - 115   2022.12

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    Language:Japanese   Publisher:The Japan Society of Veterinary Epidemiology  

    <p>Enzootic bovine leukosis is the most common neoplastic disease in cattle caused by the bovine leukemia virus (BLV). Previous studies have shown that allele type in the <i>DRB3</i> region of the bovine major histocompatibility complex (bovine leukocyte antigen: <i>BoLA</i> in cattle) is associated with resistance or susceptibility to infectious diseases. The Japanese brown (JBR) cattle have a genetic background of small population size, and thus, may exhibit different susceptibility to diseases compared with other cattle breeds. In the present study, we analyzed the diversity of the <i>BoLA-DRB3</i> gene and attempted to clarify the genetic composition of the Kumamoto and Kochi strains belonging to the JBR.</p><p>Allele typing of the <i>BoLA-DRB3</i> gene of 184 JBR detected 16 and 13 alleles in the Kumamoto and Kochi strains, respectively. The analysis of the observed (<i>H</i>o) and expected (<i>H</i>e) heterozygosity of the JBR based on allele frequencies, as well as <i>F</i>is (using <i>H</i>o and <i>H</i>e), which indicates the inbreeding coefficient within a population, showed each population to have different <i>BoLA-DRB3</i> gene diversities. Consequently, the degree of genetic difference among the populations (<i>F</i>st) was analyzed based on the allele frequencies of the <i>BoLA-DRB3</i> gene. The results showed the JBR populations to be genetically closer to the native Philippine and Myanmar cattle populations than the Japanese black, Japanese Shorthorn, Holstein or Jersey population. Furthermore, the proportions of cattle with at least on allele, which was reported to be associated with low proviral load (PVL) on infection of BLV, were 15.7% (20/127) and 63.1% (37/57) in Kumamoto and Kochi strain, respectively, and showed a significant difference. In the future, the direct evaluation of the <i>BoLA-DRB3</i> gene allele types and PVL in BLV-infected individual cattle is warranted. In addition, the PVL of BLV-infected JBR of the Kumamoto strain is known to be lower than that of other breeds in Japan. Therefore, it is necessary to investigate the possibility of a different mechanism on PVL regulation from allele types.</p><p>The results of <i>BoLA-DRB3</i> gene diversity and allele frequency analysis indicate that the JBR has a different genetic background from the Japanese black and the Shorthorn populations and possesses low PVL-associated alleles. Further studies are needed to analyze the relationship between PVL and <i>BoLA-DRB3</i> gene in JBR to clarify the susceptibility of JBR to BLV.</p>

    DOI: 10.2743/jve.26.108

  • Molecular analysis of bovine leukemia virus in early epidemic phase in Japan using archived formalin fixed paraffin embedded histopathological specimens

    NISHIKAKU Kohei, NOGUCHI Tatsuo, MURAKAMI Satoshi, TORII Yasushi, KOBAYASHI Tomoko

    Journal of Veterinary Medical Science   84 ( 3 )   350 - 357   2022

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    Language:English   Publisher:JAPANESE SOCIETY OF VETERINARY SCIENCE  

    <p>Bovine leukemia virus (BLV) is an important pathogen associated with enzootic bovine leukosis. In this study, we performed PCR and sequencing analysis to characterize BLVgp51 sequences from formalin-fixed paraffin-embedded (FFPE) specimens made from 1974 to 2000 and successfully obtained BLV proviral genome sequences from 94% of the analyzed samples. Furthermore, from these samples, we reconstructed eight full-length and nearly full-length BLVgp51 sequences. These sequences were classified as BLV genotype 1, implying that genotype1 has already been circulating in Japan since the 1970s. In our results, the proviral DNA was detected in the 1970s, 1980s, and 1990s in the same manner, indicating that the detection of BLV proviral genome depends on storage conditions rather than storage period. The sequences obtained in this study provide direct insights into BLV sequences before 2000, which serves as a good calibrator for inferring ancient BLV diversity.</p>

    DOI: 10.1292/jvms.21-0570

    PubMed

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  • Advanced maternal age induces fetal growth restriction through decreased placental inflammatory cytokine expression and immune cell accumulation in mice

    HIRATA Yoshiki, KATSUKURA Yusuke, HENMI Yuka, OZAWA Ren, SHIMAZAKI Sayaka, KUROSAWA Akira, TORII Yasushi, TAKAHASHI Hironori, IWATA Hisataka, KUWAYAMA Takehito, SHIRASUNA Koumei

    Journal of Reproduction and Development   67 ( 4 )   257 - 264   2021

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    Language:English   Publisher:The Society for Reproduction and Development  

    <p> Advanced maternal age is a risk factor for female infertility, and placental dysfunction is considered one of the causes of pregnancy complications. We investigated the effects of advanced maternal aging on pregnancy outcomes and placental senescence. Female pregnant mice were separated into three groups: young (3 months old), middle (8–9 months old), and aged (11–13 months old). Although the body weights of young and middle dams gradually increased during pregnancy, the body weight of aged dams only increased slightly. The placental weight and resorption rate were significantly higher, and live fetal weights were reduced in a maternal age-dependent manner. Although mRNA expression of senescence regulatory factors (p16 and p21) increased in the spleen of aged dams, mRNA expression of p16 did not change and that of p21 was reduced in the placenta of aged dams. Using a cytokine array of proteins extracted from placental tissues, the expression of various types of senescence-associated secretory phenotype (SASP) factors was decreased in aged dams compared with young and middle dams. The aged maternal placenta showed reduced immune cell accumulation compared with the young placenta. Our present results suggest that models using pregnant mice older than 8 months are more suitable for verifying older human pregnancies. These findings suggest that general cellular senescence programs may not be included in the placenta and that placental functions, including SASP production and immune cell accumulation, gradually decrease in a maternal age-dependent manner, resulting in a higher rate of pregnancy complications.</p>

    DOI: 10.1262/jrd.2021-034

    PubMed

    CiNii Articles

  • Phylogenomics and Spatiotemporal Dynamics of Bovine Leukemia Virus Focusing on Asian Native Cattle: Insights Into the Early Origin and Global Dissemination International coauthorship

    Kohei Nishikaku, Takahiro Yonezawa, Masahide Nishibori, Masashi Harada, Fuki Kawaguchi, Shinji Sasazaki, Yasushi Torii, Kazuhiko Imakawa , Kuniko Kawai , Jianquan Liu , Hideyuki Mannen, Tomoko Kobayashi

    Front Microbiol   13   2020.06

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  • Actinomycosis: Recent Findings of the Disease in Livestock and Companion Animals

    MURAKAMI Satoshi, KOBAYASHI Tomoko, TORII Yasushi

    Journal of the Japan Veterinary Medical Association   73 ( 4 )   177 - 184   2020.04

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    Language:Japanese   Publisher:Japan Veterinary Medical Association  

    DOI: 10.12935/jvma.73.177

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