論文 - 吉田 沙樹
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Collaborative Survey and Collection of Vegetable Genetic Resources in Myanmar, 2019 and 2020 査読あり
Saki YOSHIDA, Hidehiko KIKUNO, Than Naing Oo, Ohm Mar Saw, Kenji IRIE
Annual Report on Exploration and Introduction of Plant Genetic Resources 27 100 - 113 2021年12月
担当区分:筆頭著者 記述言語:英語 掲載種別:研究論文(学術雑誌)
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Development of simple sequence repeat (SSR) and morphological markers to identify Jaboticaba cultivars 査読あり
Takashi SHINOHARA, Shunsuke HONDA, Saki YOSHIDA, Kiyotaka OHO, Daizo IGARASHI, Kenji WAKUI, Rumi OHTAKE and Keisuke TANAKA
Tropical Agriculture and Development 65 ( 2 ) 68 - 74 2021年06月
記述言語:英語 掲載種別:研究論文(学術雑誌)
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Development of Simple Sequence Repeat (SSR) and Morphological Markers to Identify Jaboticaba Cultivars
SHINOHARA Takashi, HONDA Shunsuke, YOSHIDA Saki, OHO Kiyotaka, IGARASHI Daizo, WAKUI Kenji, OHTAKE Rumi, TANAKA Keisuke
Tropical Agriculture and Development 65 ( 2 ) 68 - 74 2021年
記述言語:英語 出版者・発行元:Japanese Society for Tropical Agriculture
<p>Jaboticaba, belonging to Myrtaceae family, has been a minor tropical fruit tree in Japan and three cultivars, ‘Sabará’, ‘Açu’ and ‘Miúda’, have been distributed. The cultivars are clearly distinguished by their bearing habits and traits of fruits. As Jaboticaba has polyembryony, it is possible to propagate the cultivars in large amounts if techniques selecting seedlings that generated from apomictic embryos exist. This research aims to develop simple sequence repeat (SSR) and morphological markers. Over 50,000 SSRs in the genome extracted from ‘Sabará’ were cataloged using the microsatellite capture sequencing technique, and one SSR primer set was developed for the identification of the three cultivars. Each unique fragment for ‘Açu’ and ‘Miúda’ in the PCR products may be non-specific fragment such as due to ascribed by different genotypes or homologues in flanking SSR region, but then the primer set indicated high robustness because of profiling consistently within and among cultivars. The three jaboticaba cultivars can be also classified by morphological traits of the leaf. ‘Sabará’ has significantly larger leaflets, which have uneven surface and come out from the rachis downwards, than other two cultivars. The size of leaflet is not different significantly between ‘Açu’ and ‘Miúda’, but their leaflet color at young stage differs clearly. The former is green, and the latter cultivar is red. These morphological traits would be influenced by ambient environments but these are practical to classify the three jaboticaba cultivars at the juvenile phase.</p>
DOI: 10.11248/jsta.65.68
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Flowering response of Myanmar mustard (Brassica juncea L.) genetic resources under different temperature and day-length conditions 査読あり
Saki YOSHIDA, Chigusa TANI, Min San Thein, Ohm Mar Saw, Kenji WAKUI, Hidehiko KIKUNO, and Kenji IRIE
Tropical Agriculture and Development 64 ( 3 ) 107 - 112 2020年10月
担当区分:筆頭著者 記述言語:英語 掲載種別:研究論文(学術雑誌)
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Collaborative Survey and Collection of Brassica Vegetable Genetic Resources in Myanmar, 2018 and 2019 査読あり
Saki YOSHIDA, Kenji WAKUI, Zin Thu Zar Maung, Than Naing Oo, Ohm Mar Saw, Kenji IRIE
Annual Report on Exploration and Introduction of Plant Genetic Resources 35 205 - 217 2020年03月
担当区分:筆頭著者 記述言語:英語 掲載種別:研究論文(学術雑誌)
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Flowering Response of Myanmar Mustard (<i>Brassica juncea</i> L.) Genetic Resources under Different Temperature and Day-length Conditions
YOSHIDA Saki, TANI Chigusa, Min San Thein, Ohm Mar Saw, WAKUI Kenji, KIKUNO Hidehiko, IRIE Kenji
Tropical Agriculture and Development 64 ( 3 ) 107 - 112 2020年
記述言語:英語 出版者・発行元:Japanese Society for Tropical Agriculture
<p>Mustard (<i>Brassica juncea</i> L.) is an important vegetable worldwide. Its floral initiation has been reported to be related to the basic vegetative growth phase, a low-temperature, and a long-day requirements. Late flowering is required for year-round production when mustard is cultivated as a leaf vegetable, but many aspects of the flowering response are unclear. Herein, we investigated the responses of mustard genetic resources, collected from a tropical and/or sub tropical area of Myanmar under natural conditions, grown in an experimental field and under different temperature and day-length conditions, controlled by a phytotron. A total of 22 accessions from Myanmar were grown in a field in Tokyo, and the earliest accessions, M39 and M109, bolted at 38 days, M116 bolted at 71 days. The number of days from sowing to bolting varied widely among the accessions, up to 33 days. Regarding low-temperature requirements, we classified the accessions into three types: low requirement for low temperature (M44), high requirement for low temperature (M15), and neutral requirement for low temperatures (M36, M42, M96, P1). We observed that accessions J1, P2, M15, M36, M42, M44, and M96 could bolt without low temperature under certain long-day conditions. Conversely, we observed that J2 was a very late-maturing cultivar that did not bolt without low temperature even under long-day conditions.The critical day length of M15, M36, M42, M44, M96, J1 and P2 was estimated to be approx. 12 hr.</p><p></p>
DOI: 10.11248/jsta.64.107
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Field Survey and Collection of “chinbaoˮ, Hibiscus spp. in Chin State of Myanmar (20th of December 2017 - 1st of January) 査読あり
Mami NAGASHIMA, Saki YOSHIDA, Hidehiko KIKUNO, Kenji WAKUI, Yoshiaki NISHIKAWA, Ohm Mar Saw, Sander Moe, Kenji IRIE
Annual Report on Exploration and Introduction of Plant Genetic Resources 34 147 - 158 2019年03月
記述言語:英語 掲載種別:研究論文(学術雑誌)
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Collaborative Survey and Collection of Brassica Vegetable Genetic Resources in Myanmar 査読あり
Saki YOSHIDA, Hidehiko KIKUNO, Kenji WAKUI, Mami NAGASHIMA, Sander Moe, Ohm Mar Saw, Kenji IRIE
Annual Report on Exploration and Introduction of Plant Genetic Resources 34 147 - 158 2019年03月
担当区分:筆頭著者 記述言語:英語 掲載種別:研究論文(学術雑誌)
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Exploration and Collection of Vegetable Genetic Resources within Brassicaceae in and around Kachin State of Myanmar 査読あり
Saki YOSHIDA, Hidehiko KIKUNO, Mami NAGASHIMA, Than Naing OO, Ohm Mar SAW, Kenji IRIE
Saki YOSHIDA 33 223 - 237 2018年03月
担当区分:筆頭著者 記述言語:英語 掲載種別:研究論文(学術雑誌)
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Effective DNA fragmentation technique for simple sequence repeat detection with a microsatellite-enriched library and high-throughput sequencing 査読あり
Keisuke TANAKA, Rumi OHTAKE, Saki YOSHIDA, Takashi SHINOHARA
Future Science Group 発行, BioTechniques 62 ( 4 ) 180 - 182 2017年04月
記述言語:英語 掲載種別:研究論文(学術雑誌)
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Complete nucleotide sequence of a monopartite Begomovirus and associated satellites infecting Carica papaya in Nepal 査読あり
Muhammad Shafiq Shahid, Saki YOSHIDA, G. B. Khatri-Chhetri, Rob W. Briddon, Keiko T. NATSUAKI
Virus Genes 46 ( 3 ) 581 - 584 2013年02月
記述言語:英語 掲載種別:研究論文(学術雑誌)